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    Generation and use of isotopic patterns in mass spectral phenotypic comparison of organisms
    74.
    发明授权
    Generation and use of isotopic patterns in mass spectral phenotypic comparison of organisms 有权
    生物体的质谱表型比较中同位素模式的生成与利用

    公开(公告)号：US08969251B2

    公开(公告)日：2015-03-03

    申请号：US12244555

    申请日：2008-10-02

    申请人： Christopher William Ward Beecher

    发明人： Christopher William Ward Beecher

    IPC分类号： B01D59/44 ,  C40B20/08 ,  H01J49/00 ,  G01N33/58 ,  G01N33/50 ,  G01N33/68

    CPC分类号： H01J49/0009 ,  B01D59/44 ,  B01J2219/00527 ,  B01J2219/00581 ,  B01J2219/00659 ,  B01J2219/0072 ,  C40B20/08 ,  G01N33/5038 ,  G01N33/5082 ,  G01N33/58 ,  G01N33/6848

    摘要： A method for assaying phenotypic similarity or dissimilarity between organisms is disclosed in which a composite sample of admixed first and second samples is provided. The first, standard sample contains average concentrations of compounds of molecular mass less than about 1000 AMU present in the organism species. The second, assay sample contains compounds of having a similar molecular mass present in the organism whose phenotype is to be assayed. The constituents of both samples are (i) in a liquid medium and (ii) each compound of a sample has the same, first and second respective amounts of first and second stable isotopes of a first atom. The composite sample is mass spectroscopically analyzed for analytes, with the ratio of first to second isotope being determined for each analyte, along with a composite sample median ratio. The ratios for each analyte are compared to the median, with outlying ratios indicating dissimilarity.

    摘要翻译： 公开了一种用于测定生物之间的表型相似性或不相似性的方法，其中提供了混合的第一和第二样品的复合样品。 第一个标准样品含有生物体中存在的分子量小于约1000AMU的化合物的平均浓度。 第二个测定样品含有在其表型要被测定的生物体中存在相似分子量的化合物。 两个样品的成分是（i）在液体介质中，（ii）样品的每种化合物具有与第一原子相同的第一和第二相应量的第一和第二稳定同位素。 复合样品对分析物进行质谱分析，每个分析物确定第一至第二同位素的比例，以及复合样品中值比。 将每个分析物的比例与中位数进行比较，其中离散比率表示不相似性。

    RANTES Multiplexed Assay, RANTES Variants Related to Disease, and RANTES Variants Related to Enzymatice Activity
    76.
    发明申请
    RANTES Multiplexed Assay, RANTES Variants Related to Disease, and RANTES Variants Related to Enzymatice Activity 审中-公开
    RANTES多重测定，与疾病相关的RANTES变体和与酶活性相关的RANTES变体

    公开(公告)号：US20130053257A1

    公开(公告)日：2013-02-28

    申请号：US13577638

    申请日：2011-02-07

    申请人： Paul E. Oran ,  Randall W. Nelson

    发明人： Paul E. Oran ,  Randall W. Nelson

    IPC分类号： C40B20/08

    CPC分类号： G01N33/6863 ,  G01N2333/523 ,  G01N2800/00

    摘要： The present invention relates to multiplexed assays for the diagnosis of RANTES-based disorders. Essentially, a single, high information content RANTES assay is used to simultaneously determine an individual's disposition towards a disease as well as the onset and progression of the disease (or response to treatment). As such, the (single) analysis has the particular advantage of always producing data useful in the longitudinal monitoring (of individuals) for a disease. In specific example, the discovery relates RANTES isoforms with the predisposition, onset and progression of T2D, CHF, MI, and cancer. All isoforms of particular blood and urine borne proteins—containing protein phenotype data—are monitored in a single, high throughput analysis able to acquire data relevant to the stages of the disease (or treatment).

    摘要翻译： 本发明涉及用于诊断基于RANTES的疾病的多重测定。 本质上，使用单一的高信息含量的RANTES测定来同时确定个体对疾病的处置以及疾病的发作和进展（或对治疗的反应）。 因此，（单一）分析具有总是产生用于疾病纵向监测（个体）的数据的特殊优点。 在具体实例中，该发现将RANTES同种型与T2D，CHF，MI和癌症的倾向，发病和进展相关联。 特异性血液和尿载蛋白质蛋白质表型数据的所有同种型 - 在能够获得与疾病（或治疗）阶段相关的数据的单个高通量分析中进行监测。

    BIOMARKER DISCOVERY IN COMPLEX BIOLOGICAL FLUID USING BEAD OR PARTICLE BASED LIBRARIES AND DIAGNOSTIC KITS AND THERAPEUTICS
    77.
    发明申请
    BIOMARKER DISCOVERY IN COMPLEX BIOLOGICAL FLUID USING BEAD OR PARTICLE BASED LIBRARIES AND DIAGNOSTIC KITS AND THERAPEUTICS 有权
    生物识别在复杂生物流体中的应用基于珠粒或基于颗粒的图书馆和诊断套件及治疗方法

    公开(公告)号：US20120270741A1

    公开(公告)日：2012-10-25

    申请号：US13428313

    申请日：2012-03-23

    申请人： Muralidhar Reddy MOOLA ,  Jessica SCHILKE

    发明人： Muralidhar Reddy MOOLA ,  Jessica SCHILKE

    IPC分类号： C40B20/08 ,  C07K7/06 ,  C40B30/04 ,  G01N33/574 ,  C40B40/10 ,  G01N33/543

    CPC分类号： G01N33/6845 ,  C07K1/047 ,  C07K7/06 ,  C40B40/04 ,  C40B40/10 ,  C40B60/12 ,  G01N33/566 ,  G01N33/57438 ,  G01N2800/104 ,  G01N2800/2821

    摘要： The present invention is useful in screening for biomarkers associated with any other disease or condition. Such diseases and conditions range from the neurological diseases, autoimmune diseases and cancers identified above as well as any other disease or condition that has a biomarker such as an antibody or other characterizing protein or biomolecule associated with the disease or progression of the disease. The large ligand libraries of the invention can be used directly in biological fluid, under the appropriate experimental conditions and according to the processes recited herein, to screen for such markers and without the need to use fewer support members (e.g. about 100,000 or less) or without the need to transfer such peptoids or ligands to a microarray before screening the biological fluid. In addition, the ligand libraries may also be used to screen for cell based receptors that specifically relate to a particular cell surface marker.

    摘要翻译： 本发明可用于筛选与任何其它疾病或病症相关的生物标志物。 这些疾病和病症范围从神经系统疾病，自身免疫性疾病和上述癌症以及具有生物标志物如抗体或其它特征性蛋白质或与该疾病或疾病进展相关的生物分子的任何其它疾病或病症。 本发明的大配体文库可以在适当的实验条件下，并根据本文所述的方法直接用于生物流体，以筛选这些标记物，而不需要使用较少的支持成员（例如约100,000或更少）或 而不需要在筛选生物流体之前将这些类似物或配体转移到微阵列。 此外，配体文库也可用于筛选特异性涉及特定细胞表面标志物的基于细胞的受体。

    METHOD FOR THE IDENTIFICATION BY MOLECULAR TECHNIQUES OF GENETIC VARIANTS THAT ENCODE NO D ANTIGEN (D-) AND ALTERED C ANTIGEN (C+W)
    80.
    发明申请
    METHOD FOR THE IDENTIFICATION BY MOLECULAR TECHNIQUES OF GENETIC VARIANTS THAT ENCODE NO D ANTIGEN (D-) AND ALTERED C ANTIGEN (C+W) 审中-公开
    通过编码无抗原（D-）和改变的抗原（C + W）的遗传变异体的分子技术鉴定方法

    公开(公告)号：US20120172239A1

    公开(公告)日：2012-07-05

    申请号：US13339058

    申请日：2011-12-28

    申请人： Jorge Ochoa ,  Monica Lopez ,  Diego Tejedor ,  Antonio Martinez ,  Laureano Simon

    发明人： Jorge Ochoa ,  Monica Lopez ,  Diego Tejedor ,  Antonio Martinez ,  Laureano Simon

    IPC分类号： C40B20/00 ,  C40B20/08 ,  C07H21/04 ,  C40B40/06 ,  C12Q1/68 ,  G01N21/64

    CPC分类号： C12Q1/6881 ,  A61K35/14 ,  C12Q2600/156

    摘要： The invention relates to genotyping and blood cell antigen determination. In particular, the invention addresses discriminating the RHD*DIIIa-CE(4-7)-D or RHD*DIIIa-CE(4-7)-D)-like blood type variants, from RHD*DIIIa, RHD*DIVa-2 and other blood type variants. The invention provides methods for genotyping a subject, comprising: a) determining at least 4 markers in a sample that has been obtained from the subject, wherein the markers comprise: (i) the presence or absence of an RHCE*C allele; (ii) the presence or absence of an RHD/RHCE hybrid exon 3 (RHD/CE Hex03) allele; (iii) the absence of, or a single nucleotide polymorphism (SNP) variant within, any one of position 602 of exon 4, position 667 of exon 5, or position 819 of exon 6 of RHD; and (iv) the absence of, or SNP variant within, position 1048 of RHD exon 7. The invention also provides probes, primers and kits for use in such methods.

    摘要翻译： 本发明涉及基因分型和血细胞抗原测定。 特别地，本发明涉及从RHD * DIIIa，RHD * DIVa-2区分RHD * DIIIa-CE（4-7）-D或RHD * DIIIa-CE（4-7）-D）样血型变异体 和其他血型变体。 本发明提供了用于对受试者进行基因分型的方法，其包括：a）确定已经从受试者获得的样品中至少4个标记，其中所述标记包括：（i）存在或不存在RHCE * C等位基因; （ii）存在或不存在RHD / RHCE混合型外显子3（RHD / CE Hex03）等位基因; （iii）在外显子4的位置602，外显子5的位置667或RHD的外显子6的位置819中的任何一个中不存在或单个核苷酸多态性（SNP）变体; 和（iv）在RHD外显子7的位置1048内不存在或SNP变体。本发明还提供用于这些方法的探针，引物和试剂盒。