Method for biochip detecting limited cells
    85.
    发明申请
    Method for biochip detecting limited cells 审中-公开
    生物芯片检测有限细胞的方法

    公开(公告)号:US20060068396A1

    公开(公告)日:2006-03-30

    申请号:US10948224

    申请日:2004-09-24

    CPC classification number: C12Q1/6837 C12Q2565/507

    Abstract: The present invention is a method for a biochip detecting limited cells. The present invention comprises steps of: obtaining a nylon membrane chip having required nucleotide fragments arranged in a dot matrix way by using a manual spotter; naturally drying by heat and fixing the nucleotide fragments on the nylon membrane chip by a rapid nucleic acid cross-linker when preparing a chip; collecting some normal whole blood to be linearly amplified; synthesizing required amount of cDNA by a reverse transcription to obtain a marker as a probe; processing labeling, hybridization and post-hybridization to the chip and the marker; processing chemical color reaction; and automatically analyzing the result image after the chemical color reaction. Accordingly, a gene biochip operation technology platform with low cost, easy operation and high efficiency is obtained. And so the functions and the applications of the gene biochip can be effectively worked out and the practical applications of the gene biochip on related fields can be conclusively popularized.

    Abstract translation: 本发明是一种检测有限细胞的生物芯片的方法。 本发明包括以下步骤:通过使用手动打斑机获得具有以点阵方式布置的所需核苷酸片段的尼龙膜芯片; 通过加热自然干燥,并在制备芯片时通过快速核酸交联剂固定尼龙膜芯片上的核苷酸片段; 收集一些正常全血要线性扩增; 通过逆转录合成所需量的cDNA以获得作为探针的标记; 加工标记,杂交和后期杂交到芯片和标记物; 加工化学色素反应; 并在化学反应后自动分析结果图像。 因此,获得了低成本,易于操作和高效率的基因生物芯片操作技术平台。 因此可以有效地制定基因生物芯片的功能和应用,基因生物芯片在相关领域的实际应用可以得到最终推广。

    Ceramic-metal composite and method to form said composite
    86.
    发明授权
    Ceramic-metal composite and method to form said composite 有权
    陶瓷 - 金属复合材料和形成所述复合材料的方法

    公开(公告)号:US06296045B1

    公开(公告)日:2001-10-02

    申请号:US09133109

    申请日:1998-08-12

    Abstract: A ceramic-metal composite that is tough and stiff has been prepared and is comprised of an inert ceramic (e.g., alumina) embedded and dispersed in a matrix comprised of a metal (e.g., aluminum), a reactive ceramic (e.g., boron carbide) and a reactive ceramic-metal reaction product (e.g., AlB2, Al4BC, Al3B48C2, AlB12, Al4C3, AlB24C4 or mixtures thereof) wherein grains of the inert ceramic have an average grain size greater than or equal to the average grain size of grains of the reactive ceramic. The ceramic-metal composite may be prepared by forming a mixture comprised of an inert ceramic powder (e.g., alumina) and a reactive ceramic powder (e.g., boron carbide), the inert ceramic powder having an average particle size equal to or greater than the average particle size of the reactive ceramic powder, forming the mixture into a porous body and consolidating the porous body in the presence of a metal (e.g., aluminum) to form the ceramic-metal composite.

    Abstract translation: 已经制备了韧性和刚性的陶瓷 - 金属复合材料,其包括嵌入和分散在由金属(例如铝),反应性陶瓷(例如碳化硼)组成的基体中的惰性陶瓷(例如氧化铝) 和反应性陶瓷 - 金属反应产物(例如,AlB2,Al4BC,Al3B48C2,AlB12,Al4C3,AlB24C4或其混合物),其中惰性陶瓷的晶粒的平均晶粒尺寸大于或等于晶粒的平均晶粒尺寸 活性陶瓷。 陶瓷 - 金属复合材料可以通过形成由惰性陶瓷粉末(例如氧化铝)和反应性陶瓷粉末(例如碳化硼)组成的混合物来制备,惰性陶瓷粉末的平均粒度等于或大于 反应性陶瓷粉末的平均粒度,将混合物形成多孔体,并在金属(例如铝)的存在下固化多孔体以形成陶瓷 - 金属复合材料。

    Method for loading sub-processor in system having a plurality of
sub-processors with respect to main processor
    87.
    发明授权
    Method for loading sub-processor in system having a plurality of sub-processors with respect to main processor 失效
    在具有相对于主处理器的多个子处理器的系统中加载子处理器的方法

    公开(公告)号:US6079017A

    公开(公告)日:2000-06-20

    申请号:US982572

    申请日:1997-12-02

    CPC classification number: H04L69/329

    Abstract: A method for loading sub-processors in system having a plurality of sub-processors connected with main processor via a repeater and utilizing broadcasting function is disclosed. The method comprises the Steps of inspecting a transfer path communicating the main processor and the plural sub-processors; collecting a loading request signal transmitted from the sub-processors at predetermined time period; and loading simultaneously the sub-processors using broadcasting function after interconnecting the sub-processors which request the collected loading during the collecting Step with the main processor via the repeater in multipoint base. The sub-processors having the collected signal are simultaneously loaded using broadcasting function, it has the effect for reducing loading time.

    Abstract translation: 公开了一种用于在具有通过中继器连接主处理器并利用广播功能的多个子处理器的系统中加载子处理器的方法。 该方法包括检查与主处理器和多个子处理器通信的传送路径的步骤; 收集在预定时间段从子处理器发送的加载请求信号; 并且在将在收集步骤期间请求收集的负载的子处理器与多个基站之间的中继器与主处理器互连之后,同时使用广播功能加载子处理器。 具有收集信号的子处理器使用广播功能同时加载,具有减少加载时间的效果。

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