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公开(公告)号：US10266563B2
公开(公告)日：2019-04-23
申请号：US15308327
申请日：2015-06-04
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Jean-Luc Maloisel , Ola Lind , Bjorn Noren , Ronnie Palmgren
Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.
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公开(公告)号：US10208091B2
公开(公告)日：2019-02-19
申请号：US15526849
申请日：2015-12-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC: C07K14/195 , C07K1/22 , C07K16/00
Abstract: A kappa light chain-binding polypeptide comprising or consisting essentially of one or more binding domains of Peptostreptococcus Protein L, each of said domains being selected from the group consisting of domain 2, domain 3 and domain 4.
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公开(公告)号：US20180327512A1
公开(公告)日：2018-11-15
申请号：US15525688
申请日：2015-11-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
IPC: C07K16/46 , B01J20/289 , B01J20/32 , B01D15/38
CPC classification number: C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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公开(公告)号：US20180243665A1
公开(公告)日：2018-08-30
申请号：US15753341
申请日：2016-08-18
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Karol Maciej Lacki , Hans Blom , Helena Skoglar , Linus Laurin
CPC classification number: B01D15/3809 , B01J20/24 , B01J20/28004 , B01J20/28011 , B01J20/28019 , B01J20/286 , B01J20/3212 , B01J20/3274 , B01J20/3278 , B01J20/3293 , B01J2220/603 , C07K1/22 , C07K16/065
Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm. The invention further discloses a method of separation of antibodies by affinity chromatography which employs the said separation matrix within a chromatography column.
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公开(公告)号：US20180094024A1
公开(公告)日：2018-04-05
申请号：US15348699
申请日：2016-11-10
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Annika Kristina FORSS , Gustav Jose RODRIGO , Tomas BJORKMAN , Jesper Ulf Hansson , Mats Ander
IPC: C07K1/22 , C07K16/12 , C07K17/10 , B01J20/286
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of:a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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Patent Agency Ranking
公开(公告)号：US20170334954A1
公开(公告)日：2017-11-23
申请号：US15526941
申请日：2015-11-16
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
CPC classification number: C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20170247407A1
公开(公告)日：2017-08-31
申请号：US15596040
申请日：2017-05-16
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Hans J. Johansson , Ronnie Palmgren
IPC: C07K1/22 , B01D15/38 , B01J20/285 , C07K14/31 , B01J20/289
CPC classification number: C07K1/22 , B01D15/34 , B01D15/3809 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3274 , C07K14/245 , C07K14/31 , C07K2319/00
Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.
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公开(公告)号：US20170182432A1
公开(公告)日：2017-06-29
申请号：US15458667
申请日：2017-03-14
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Klaus Gebauer
CPC classification number: B01D15/206 , B01D15/14 , B01D15/22 , G01N30/56 , G01N30/6021 , G01N30/603 , G01N30/606 , G01N2030/565
Abstract: The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.
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公开(公告)号：US09597611B2
公开(公告)日：2017-03-21
申请号：US13672832
申请日：2012-11-09
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Klaus Gebauer
CPC classification number: B01D15/206 , B01D15/14 , B01D15/22 , G01N30/56 , G01N30/6021 , G01N30/603 , G01N30/606 , G01N2030/565
Abstract: A method for providing an aseptic chromatography column includes the steps of pre-sterilizing an empty chromatography column, pre-sterilizing a chromatography medium, introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thus providing an aseptic chromatography column comprising chromatography medium.
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公开(公告)号：US20170051010A1
公开(公告)日：2017-02-23
申请号：US15308327
申请日：2015-06-04
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Jean-Luc Maloisel , Ola Lind , Bjorn Noren , Ronnie Palmgren
CPC classification number: C07K1/22 , C07K1/145 , C07K16/00 , C07K16/065 , C07K2317/55
Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.
Abstract translation: 本发明涉及在用于纯化目标分子的色谱方法（优选单克隆抗体mAb）中通过使用具有薄的外层和核心功能化的专门设计的色谱珠来去除大污染物如病毒的方法 具有吸附mAb或其部分的配体。
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