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    MICROORGANISMS AND METHODS FOR REDUCING BY-PRODUCTS
    83.
    发明公开
    MICROORGANISMS AND METHODS FOR REDUCING BY-PRODUCTS 审中-公开

    公开(公告)号：US20230287435A1

    公开(公告)日：2023-09-14

    申请号：US17772990

    申请日：2020-10-28

    Applicant: Genomatica, Inc.

    Inventor： Harish Nagarajan ,  Gionata Scalcinati ,  Tae Hoon Yang

    IPC: C12N15/52 ,  C12P7/18

    CPC classification number: C12N15/52 ,  C12P7/18 ,  C12Y202/01006 ,  C12Y104/99001 ,  C12Y501/01001 ,  C12Y102/01003 ,  C12Y602/01001 ,  C12Y203/03001

    Abstract: The present disclosure provides microbial organisms having decreased production of unwanted by-products (e.g, pyruvate-, CO2—, TCA-derived by-products; acetate; ethanol; and/or, alanine) to enhance carbon flux through acetyl-CoA, which can increase production of acetyl-CoA derived compounds (e.g, 1,3-BDO, MMA, and (3R)-hydroxybutyl (3R)-hydroxybutyrate, or any other acetyl-CoA derived compounds), and products made from any of these compounds. Also provided are one or more exogenous nucleic acids encoding enzymes that can decrease production of unwanted by-products (e.g, aldehyde dehydrogenase, acetyl-CoA synthase, amino acid dehydrogenase, alanine racemase, and/or citrate synthase), and/or one or more gene attenuations occurring in genes (e.g., acetolactate synthase) that result in decreased production of unwanted by-products. Various combinations of the exogenous nucleic acids and gene deletions are also provided in the present disclosure. Methods of making and using the same, including methods for culturing cells, and for the production of the various products are also provided.

    CANNABINOID SYNTHASE VARIANTS AND METHODS FOR THEIR USE
    84.
    发明公开
    CANNABINOID SYNTHASE VARIANTS AND METHODS FOR THEIR USE 审中-公开

    公开(公告)号：US20230167468A1

    公开(公告)日：2023-06-01

    申请号：US17995778

    申请日：2021-04-13

    Applicant: GENOMATICA, INC.

    Inventor： Deqiang ZHANG ,  Jamison Parker HUDDLESTON ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN

    IPC: C12P7/42 ,  C12N9/02 ,  C12P17/06 ,  C12P7/22

    CPC classification number: C12P7/42 ,  C12N9/0004 ,  C12Y121/03008 ,  C12Y121/03007 ,  C12P17/06 ,  C12P7/22

    Abstract: The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase Δ9-tetrahydrocannabinolic acid synthase (THCAS), comprising three alpha helices (αA, αB and αC) where a disulfide bond is not formed between alpha helix αA and alpha helix αC, wherein the non-natural cannabinoid synthase catalyzes the oxidative cyclization of cannabigerolic acid (CBGA) into a cannabinoid. The invention further relates to a non-natural Δ9-tetrahydrocannabinolic acid synthase (THCAS), a non-natural cannabidiolic acid synthase (CBDAS), and a non-natural cannabichromenic acid synthase (CBCAS) comprising at least one amino acid variation as compared to a wild type THCAS, CBDAS, or CBCAS, respectively, comprising three alpha helices (αA, αB and αC) and wherein a disulfide bond is not formed between alpha helix αA and alpha helix αC. The invention also relates to a nucleic acid, expression construct, and engineered cell for making the non-natural THCAS, CBDAS, and/or CBCAS. Also provided are compositions comprising the non-natural THCAS, CBDAS, and/or CBCAS; isolated non-natural THCAS, CBDAS, and/or CBCAS enzymes; methods of making the isolated enzymes; cell extracts comprising cannabinoids; and methods of making cannabinoids.

    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS
    86.
    发明申请
    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 有权

    公开(公告)号：US20230134936A1

    公开(公告)日：2023-05-04

    申请号：US17749015

    申请日：2022-05-19

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    IPC: C12N15/52 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12P7/52 ,  C12P17/04 ,  B01D3/00 ,  C12P7/18 ,  C12P7/42 ,  C12N15/70 ,  C12N15/81

    Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

    Aldehyde dehydrogenase variants and methods of using same
    87.
    发明授权
    Aldehyde dehydrogenase variants and methods of using same 有权

    公开(公告)号：US11634692B2

    公开(公告)日：2023-04-25

    申请号：US17280181

    申请日：2019-09-25

    Applicant: GENOMATICA, INC.

    Inventor： Amit Shah ,  Joseph Warner

    IPC: C12N9/02 ,  C12N15/90 ,  C12P7/04 ,  C12P7/62 ,  C12P13/02

    Abstract: The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID
    88.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID 有权

    公开(公告)号：US20230022727A1

    公开(公告)日：2023-01-26

    申请号：US17527902

    申请日：2021-11-16

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

    IPC: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/12

    Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

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