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91 条记录 (耗时 0.034 秒)

    Retainer ring having air deflector and airbag mounting structure using the same
    82.
    发明授权
    Retainer ring having air deflector and airbag mounting structure using the same 有权
    具有导风板的保持环和使用其的安全气囊安装结构

    公开(公告)号：US06364342B1

    公开(公告)日：2002-04-02

    申请号：US09478178

    申请日：2000-01-05

    申请人： Joon-ho Kim

    发明人： Joon-ho Kim

    IPC分类号： B60R2126

    CPC分类号： B60R21/217

    摘要： A retainer ring having an air deflector, for use with an airbag mounting structure, includes a flange in which an inflater opening for receiving an inflater is formed; and a wall, a base of which is connected to the flange, extending upward and outward from the inflater opening at a predetermined angle to diffuse and deflect exploding gas upward. The wall, which realizes a simplified configuration for the retainer ring, may include convex portions projecting inwardly towards the inflater opening, being regularly spaced, and rib formations integrally formed on an inner side of the wall between the convex portions. The rib formations are evenly spaced, as a pair of rows covering the height of the inner side of the wall, and slope downward on either side of the convex portions. The airbag mounting structure clamps the inflater and the airbag between the retainer ring and a baseplate, which is mounted to a support within an automobile. To determine a mounting position and orientation, a tabbed cut-out is formed in the retainer ring, juxtaposed to a coupling hole thereof, and is bent at a right angle to pass through an alignment notch formed in a flange of the inflater and a series of alignment holes formed in the other components.

    摘要翻译： 具有用于气囊安装结构的导风板的保持环包括其中形成有用于接收充气器的充气器开口的凸缘; 并且其底部连接到凸缘的壁，其以预定角度从充气器开口向上和向外延伸，以将爆炸气体向上扩散和偏转。 实现保持环的简化构造的壁可以包括向内朝向充气器开口向内突出的凸起部分，其规则地间隔开，并且肋构形一体地形成在凸起部分之间的壁的内侧上。 肋骨形成均匀的间隔，作为覆盖壁的内侧的高度的一对排，并且在凸部的任一侧向下倾斜。 安全气囊安装结构将充气器和气囊夹在保持环和安装到汽车内的支撑件的基板之间。 为了确定安装位置和方向，在保持环中形成有与其联接孔并列的标签切口，并且以直角弯曲以穿过形成在充气器的凸缘中的对准凹口和一系列 的对准孔形成在其他部件中。

    Fluorescence detecting optical system and multi-channel fluorescence detection apparatus having the same
    83.
    发明授权
    Fluorescence detecting optical system and multi-channel fluorescence detection apparatus having the same 有权
    荧光检测光学系统和具有该荧光检测光学系统的多通道荧光检测装置

    公开(公告)号：US08624201B2

    公开(公告)日：2014-01-07

    申请号：US13421119

    申请日：2012-03-15

    申请人： Kyung-ho Kim ,  Joon-ho Kim ,  Kak Namkoong ,  Won-seok Chung

    发明人： Kyung-ho Kim ,  Joon-ho Kim ,  Kak Namkoong ,  Won-seok Chung

    IPC分类号： G01N21/64

    CPC分类号： G01N21/645 ,  G01N21/6445

    摘要： A fluorescence detecting optical system, and a multi-channel fluorescence detection apparatus comprising same, comprising a light source that emits excitation light, a polarizer that transmits light having a predetermined polarization component, a polarizing beam splitter that transmits light having a predetermined polarization component and reflects light having a different polarization component than the predetermined polarization component, and a quarter-wave plate converting linearly polarized to circularly polarized light or vice versa.

    摘要翻译： 一种荧光检测光学系统和包括其的多通道荧光检测装置，包括发射激发光的光源，透射具有预定偏振分量的光的偏振器，透射具有预定偏振分量的光的偏振分束器和 反射具有与预定偏振分量不同的偏振分量的光，以及将线偏振成圆偏振光的四分之一波片反射。

    Method and apparatus for amplifying nucleic acids
    84.
    发明授权
    Method and apparatus for amplifying nucleic acids 有权
    扩增核酸的方法和装置

    公开(公告)号：US07943348B2

    公开(公告)日：2011-05-17

    申请号：US12504271

    申请日：2009-07-16

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

    Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus
    86.
    发明授权
    Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
    使用电渗析法调节盐浓度的装置，包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法

    公开(公告)号：US07722751B2

    公开(公告)日：2010-05-25

    申请号：US11440872

    申请日：2006-05-25

    申请人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

    发明人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

    IPC分类号： B01D61/48

    CPC分类号： B01D61/422 ,  B01D61/46 ,  B01L3/5027 ,  G01N2001/4011

    摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.

    摘要翻译： 提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。 该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。 即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

    METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS
    87.
    发明申请
    METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS 有权
    用于放大核酸的方法和装置

    公开(公告)号：US20100021972A1

    公开(公告)日：2010-01-28

    申请号：US12504271

    申请日：2009-07-16

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

    METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT
    89.
    发明申请
    METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT 有权
    使用KOSMOTROPIC盐净化RNA的方法

    公开(公告)号：US20090048437A1

    公开(公告)日：2009-02-19

    申请号：US12110391

    申请日：2008-04-28

    申请人： Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

    发明人： Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

    IPC分类号： C07H21/02

    CPC分类号： C07H21/02 ,  C12N15/1003 ,  C12N15/1006

    摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

    摘要翻译： 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。

    Sensitivity enhanced biomolecule field effect transistor
    90.
    发明授权
    Sensitivity enhanced biomolecule field effect transistor 有权
    灵敏度增强的生物分子场效应晶体管

    公开(公告)号：US07151301B2

    公开(公告)日：2006-12-19

    申请号：US11057642

    申请日：2005-02-14

    申请人： Kyu-tae Yoo ,  Geun-bae Lim ,  Joon-ho Kim ,  Kak Namkoong

    发明人： Kyu-tae Yoo ,  Geun-bae Lim ,  Joon-ho Kim ,  Kak Namkoong

    IPC分类号： H01L29/76

    CPC分类号： G01N27/4145

    摘要： There is provided a biomolecule FET enhancing a sensitivity. The biomolecule FET includes a substrate, first and second impurity regions formed on both sides of the substrate, and doped with impurities of a polarity opposite to that of the substrate, a gate formed on the substrate and being in contact with the first and second impurity regions, and a probe biomolecule attached to the gate. A region of the gate adjacent to the first impurity region is wider than a region thereof adjacent to the second impurity region. A density of the probe biomolecule attached to the surface of the gate is increased, and when detecting a level of hybridization of the probe biomolecule and the target biomolecule, its sensitivity is improved.

    摘要翻译： 提供了提高灵敏度的生物分子FET。 生物分子FET包括基板，形成在基板两侧的第一和第二杂质区，并且掺杂极性与基板极性相反的杂质;形成在基板上并与第一和第二杂质接触的栅极 区域，以及连接到门的探针生物分子。 与第一杂质区相邻的栅极的区域比与第二杂质区相邻的区域宽。 附着在栅极表面的探针生物分子的密度增加，并且当检测到探针生物分子和靶生物分子的杂交水平时，其灵敏度得到改善。