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    Method and apparatus for amplifying nucleic acids
    1.
    发明申请
    Method and apparatus for amplifying nucleic acids 有权
    扩增核酸的方法和装置

    公开(公告)号：US20050202489A1

    公开(公告)日：2005-09-15

    申请号：US11071383

    申请日：2005-03-03

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12N15/09 ,  B01F5/06 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/38 ,  C12N15/00 ,  C12Q1/68 ,  G01N35/00 ,  G01N35/08 ,  C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： Provided are a method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 提供了用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

    Method and apparatus for amplifying nucleic acids
    3.
    发明授权
    Method and apparatus for amplifying nucleic acids 有权
    扩增核酸的方法和装置

    公开(公告)号：US07943348B2

    公开(公告)日：2011-05-17

    申请号：US12504271

    申请日：2009-07-16

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

    METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS
    5.
    发明申请
    METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS 有权
    用于放大核酸的方法和装置

    公开(公告)号：US20100021972A1

    公开(公告)日：2010-01-28

    申请号：US12504271

    申请日：2009-07-16

    申请人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    发明人： Yoon-kyoung Cho ,  Joon-ho Kim ,  Kak Namkoong ,  Geun-bae Lim ,  Jun-hong Min

    IPC分类号： C12P19/34

    CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

    摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

    摘要翻译： 用于扩增核酸的方法和装置。 该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液 通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。 该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到 反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器， 以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

    Method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or amino group
    7.
    发明申请
    Method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or amino group 审中-公开
    使用涂覆有羧基或氨基的固相材料来扩增核酸的方法

    公开(公告)号：US20050186602A1

    公开(公告)日：2005-08-25

    申请号：US11042376

    申请日：2005-01-25

    申请人： Joon-ho Kim ,  Yoon-kyoung Cho ,  Jung-joo Hwang ,  Geun-bae Lim ,  Jeong-gun Lee

    发明人： Joon-ho Kim ,  Yoon-kyoung Cho ,  Jung-joo Hwang ,  Geun-bae Lim ,  Jeong-gun Lee

    IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/1006

    摘要： A method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or an amino group is provided. The method includes contacting a mixture of a nucleic acid containing sample and a salt solution with the solid phase material coated with a carboxyl group or an amino group to form a nucleic acid-solid phase material complex, washing the nucleic acid-solid phase material complex with a wash buffer, and adding a reaction solution for amplifying a nucleic acid to the nucleic acid-solid phase material complex to perform an amplification reaction.

    摘要翻译： 提供了使用涂覆有羧基或氨基的固相材料来扩增核酸的方法。 该方法包括将含有核酸的样品和盐溶液的混合物与涂覆有羧基或氨基的固相材料接触以形成核 - 固相物质络合物，洗涤核酸 - 固相物质络合物 用洗涤缓冲液加入用于将核酸扩增至核酸 - 固相物质络合物的反应溶液进行扩增反应。