公开(公告)号：US20120231499A1

公开(公告)日：2012-09-13

申请号：US13124818

申请日：2011-03-11

申请人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jeong Wook Lee ,  Young Hwan Park

发明人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jeong Wook Lee ,  Young Hwan Park

IPC分类号： C12P21/02 ,  D01D5/06 ,  C07K14/00

CPC分类号： C07K14/43518 ,  D01D5/0007 ,  D01D5/06 ,  D01F4/02

摘要： A high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications.

摘要翻译： 具有与天然丝蛋白基本相似的分子量的高分子量重组丝或丝状蛋白质，以及由其制备的具有改善的物理性质的微米或纳米尺寸的蜘蛛丝或丝状纤维 。 根据本发明的重组丝或丝状蛋白具有高分子量，如来自蜘蛛的拉丝丝蛋白，而由其制备的纤维与由天然丝蛋白产生的纤维相比具有优异的物理性能。 因此，重组丝或丝状蛋白质以及由其生产的蜘蛛丝或丝状纤维在各种工业应用中非常有用，包括生物工程应用和医疗应用。

公开(公告)号：US20120190088A1

公开(公告)日：2012-07-26

申请号：US13384576

申请日：2010-07-19

申请人： Sang Yup Lee ,  Yong Jun Choi

发明人： Sang Yup Lee ,  Yong Jun Choi

IPC分类号： C12P7/64

CPC分类号： C12N9/20 ,  C12P7/649 ,  C12Y301/01003 ,  Y02E50/13 ,  Y02P20/52

摘要： The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel.

摘要翻译： 本发明涉及使用具有生产油的能力的微生物生产脂肪酸烷基酯的方法，更具体地说，涉及生产脂肪酸烷基酯的方法，该方法包括培养具有生产油的微生物，从而 在微生物中积聚大量的油，引起微生物中产生的油的自溶以产生游离脂肪酸，并将游离脂肪酸转化为烷基酯。 根据本发明的方法，可以使用代谢工程方法将积累在微生物中的微生物油（例如由微生物产生的典型油的三酰基甘油）的油转化为高效率的脂肪酸烷基酯。 因此，本发明的方法可用于最近被发现作为生物柴油有效的脂肪酸烷基酯的工业生产。

公开(公告)号：US20120171737A1

公开(公告)日：2012-07-05

申请号：US13381340

申请日：2010-06-30

申请人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Hye Ok Kang

发明人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Hye Ok Kang

IPC分类号： C12P7/62 ,  C07H21/04 ,  C12N15/63 ,  C12N9/00 ,  C12N1/21

CPC分类号： C12P7/625 ,  C12N9/13 ,  C12P7/56 ,  C12Y208/03001

摘要： Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase.

摘要翻译： 提供能够合成乳酸聚合物（PLA）和乳酸共聚物（PLA共聚物）的各种聚羟基链烷酸酯（PHA）合成酶的突变体，以及使用其制备乳酸盐聚合物和乳酸共聚物的方法。 更具体地，提供了SEQ ID NO：2,4,6或8所示的聚羟基链烷酸酯合酶的突变体，以及使用合成酶突变体制备乳酸盐聚合物和乳酸共聚物的方法。 SEQ ID NO：2,4,6或8所示的聚羟基链烷酸酯合酶可以通过影响乳酸聚合物合成活性的氨基酸序列突变而具有乳酸聚合物和乳酸共聚物的合成活性， 乳酸聚合物和具有不同特征的共聚物，分别通过使用合成酶的突变体。

公开(公告)号：US07951558B2

公开(公告)日：2011-05-31

申请号：US11995689

申请日：2005-07-15

申请人： Sang Yup Lee ,  Jong Hyun Choi

发明人： Sang Yup Lee ,  Jong Hyun Choi

IPC分类号： C12P21/06 ,  C12N15/00 ,  C12N1/20

CPC分类号： C07K14/245 ,  C12N15/70

摘要： The present invention relates to a method for secreting and producing a target protein into cell culture broth. More particularly, the invention relates to a microorganism co-transformed with a recombinant expression vector containing E. coli outer membrane protein F (OmpF) and a recombinant expression vector containing a target protein to be secreted into cell culture broth, as well as a method of secreting and producing the target protein into cell culture broth by culturing the microorganism. According to the invention, the target protein can be secreted into cell culture broth in a pure form without fusion with other proteins so that the efficient isolation and purification of the target protein is possible.

摘要翻译： 本发明涉及一种在细胞培养液中分泌和产生靶蛋白的方法。 更具体地说，本发明涉及与含有大肠杆菌外膜蛋白F（OmpF）的重组表达载体和含有分泌到细胞培养液中的靶蛋白的重组表达载体共转化的微生物，以及一种方法 通过培养微生物将靶蛋白分泌并产生到细胞培养液中。 根据本发明，靶蛋白可以以纯形式分泌到细胞培养肉汤中，而不与其它蛋白质融合，因此靶蛋白的有效分离和纯化是可能的。

公开(公告)号：US20110124131A1

公开(公告)日：2011-05-26

申请号：US12301581

申请日：2007-04-17

申请人： Sang Yup Lee ,  Tae Jung Park

发明人： Sang Yup Lee ,  Tae Jung Park

IPC分类号： G01N33/553 ,  C12P3/00 ,  B32B5/16 ,  C12N1/21 ,  C12N1/16 ,  C12N1/00 ,  B05D7/14 ,  C12N1/15

CPC分类号： C07K14/825 ,  B82Y5/00 ,  B82Y15/00 ,  C12N9/104 ,  C12P3/00 ,  G01N33/531 ,  G01N33/588 ,  Y10T428/2982

摘要： The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, en the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores.

摘要翻译： 本发明涉及使用重金属结合蛋白制备重金属纳米粒子的方法。 更具体地，涉及一种制备重金属结构体的方法，包括以下步骤：在含重金属离子的培养基中培养用重金属结合蛋白的基因转化的微生物，以在微生物中产生重金属结构 ; 并收集所生产的重金属结构物，以及根据所述方法制备的重金属结构纳米颗粒。 与通过物理结合金属材料制备量子点的现有方法不同，本文公开的量子点可以通过在细胞中表达重金属结合蛋白而有效地产生。 此外，量子点是有用的，因为它们可以解决作为有机荧光团的缺点的光学稳定性问题。

公开(公告)号：US07897366B2

公开(公告)日：2011-03-01

申请号：US11740390

申请日：2007-04-26

申请人： Sang Yup Lee ,  Seung-Hwan Lee

发明人： Sang Yup Lee ,  Seung-Hwan Lee

IPC分类号： C12N15/09 ,  C12N15/70

CPC分类号： C12N15/1037 ,  C07K2319/03 ,  C07K2319/035 ,  C12N15/62

摘要： The present invention relates to a method for simultaneously surface expressing a target protein using a cofactor and an enzyme regenerating the cofactor on the cell surface. According to the present invention, it is possible to provide a microorganism capable of simultaneously surface expressing a target protein using a cofactor to transform a biochemical material at a high efficiency and an enzyme generating the cofactor without adding an expensive cofactor in a large amount.

摘要翻译： 本发明涉及使用辅因子和在细胞表面再生辅因子的酶同时表达表达靶蛋白的方法。 根据本发明，可以提供一种能够使用辅因子同时表达表达目标蛋白的微生物，以高效率地转化生化材料，并且可以提供生成辅因子的酶，而不会大量添加昂贵的辅因子。

公开(公告)号：US20110003349A1

公开(公告)日：2011-01-06

申请号：US12160126

申请日：2007-07-02

申请人： Sang Yup Lee ,  Kwang Ho Lee ,  Jin Hwan Park ,  Tae Yong Kim

发明人： Sang Yup Lee ,  Kwang Ho Lee ,  Jin Hwan Park ,  Tae Yong Kim

IPC分类号： C12P13/08 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19

CPC分类号： C12P13/08

摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.

摘要翻译： 本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和 使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。 通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

公开(公告)号：US20100035362A1

公开(公告)日：2010-02-11

申请号：US12410071

申请日：2009-03-24

申请人： Sang Yup Lee ,  Tae Jung Park ,  Yang Kyu Choi ,  Bon Sang Gu ,  Jae Hyuk Ahn

发明人： Sang Yup Lee ,  Tae Jung Park ,  Yang Kyu Choi ,  Bon Sang Gu ,  Jae Hyuk Ahn

IPC分类号： G01N33/53 ,  G01N33/00 ,  C07K14/00 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12P21/00

CPC分类号： G01N33/552 ,  G01N2035/00158

摘要： The present invention relates to a bio-silica chip comprising a silica-binding protein and a fabrication method thereof, and more particularly to a bio-silica chip in which a fusion protein of a silica-binding protein and a probe protein is immobilized on a chip comprising a silica layer, a fabrication method thereof and a method of using the bio-silica chip to detect interactions with biomaterials. The bio-silica chip will be very useful in biosensors, etc., because the bio-silica chip is advantageous in that it does not cause non-specific protein binding in the detection of protein-DNA, protein-ligand, protein-antibody, protein-peptide, protein-carbohydrate, protein-protein and cell-biomaterial interactions. Also, in the method for fabricating the bio-silica chip, a probe chip can be selectively immobilized on a silica device chip, which is widely used in biosensors, without a chemical surface treatment process. Thus, a chip fabricating process is simplified and a complicated process for purifying the probe protein becomes unnecessary, thus providing great improvements in productivity and economic efficiency

摘要翻译： 本发明涉及包含二氧化硅结合蛋白的生物二氧化硅芯片及其制造方法，更具体地说，涉及将二氧化硅结合蛋白质和探针蛋白质的融合蛋白质固定在其上的生物二氧化硅芯片 包括二氧化硅层的芯片，其制造方法以及使用生物二氧化硅芯片来检测与生物材料的相互作用的方法。 生物二氧化硅芯片在生物传感器等方面非常有用，因为生物二氧化硅芯片是有利的，因为它不会在检测蛋白质-DNA，蛋白质 - 配体，蛋白质 - 抗体等过程中引起非特异性蛋白质结合， 蛋白质 - 肽，蛋白质 - 碳水化合物，蛋白质 - 蛋白质和细胞 - 生物材料相互作用。 此外，在制造生物二氧化硅芯片的方法中，探针芯片可以选择性地固定在广泛用于生物传感器的二氧化硅器件芯片上，而无需化学表面处理工艺。 因此，简化了芯片制造工艺，并且不需要用于纯化探针蛋白的复杂过程，从而提高了生产率和经济效率

公开(公告)号：US07588934B2

公开(公告)日：2009-09-15

申请号：US11228927

申请日：2005-09-16

申请人： Sang Yup Lee

发明人： Sang Yup Lee

IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/64 ,  C12N15/74

CPC分类号： C12P7/46 ,  C12N9/88

摘要： A nucleotide sequence encoding a fumarate hydratase C and a method for preparing succinic acid using the same, more particularly, a fumarate hydratase C having the activity of converting malate to fumarate, a fumC nucleotide sequence encoding the fumarate hydratase C, a recombinant vector containing the nucleotide sequence, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.

摘要翻译： 编码富马酸水合酶C的核苷酸序列和使用该琥珀酸的琥珀酸的制备方法，特别是具有将苹果酸向富马酸转化的活性的富马酸盐水合酶C，编码富马酸水合酶C的重组载体， 核苷酸序列，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

公开(公告)号：US07572615B2

公开(公告)日：2009-08-11

申请号：US11738463

申请日：2007-04-20

申请人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Sung Won Lim

发明人： Sang Yup Lee ,  Hyohak Song ,  Yu Sin Jang ,  Sung Won Lim

IPC分类号： C12N9/00 ,  C12P21/00

CPC分类号： C12P7/46 ,  C12N9/0008

摘要： Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.

摘要翻译： 编码甲酸脱氢酶D＆E的核苷酸序列和使用其编码琥珀酸的方法，更具体地说，将甲酸转化为二氧化碳和氢的甲酸脱氢酶D＆E，编码甲酸脱氢酶D＆E的fdhD和fdhE核苷酸序列，重组载体 含有核苷酸序列，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。