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    PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS
    1.
    发明申请
    PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS 审中-公开
    使用聚羟基烷基酯合成酶的乳酸聚合物和乳酸共聚物的制备方法

    公开(公告)号：US20120171737A1

    公开(公告)日：2012-07-05

    申请号：US13381340

    申请日：2010-06-30

    申请人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Hye Ok Kang

    发明人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Hye Ok Kang

    IPC分类号： C12P7/62 ,  C07H21/04 ,  C12N15/63 ,  C12N9/00 ,  C12N1/21

    CPC分类号： C12P7/625 ,  C12N9/13 ,  C12P7/56 ,  C12Y208/03001

    摘要： Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase.

    摘要翻译： 提供能够合成乳酸聚合物（PLA）和乳酸共聚物（PLA共聚物）的各种聚羟基链烷酸酯（PHA）合成酶的突变体，以及使用其制备乳酸盐聚合物和乳酸共聚物的方法。 更具体地，提供了SEQ ID NO：2,4,6或8所示的聚羟基链烷酸酯合酶的突变体，以及使用合成酶突变体制备乳酸盐聚合物和乳酸共聚物的方法。 SEQ ID NO：2,4,6或8所示的聚羟基链烷酸酯合酶可以通过影响乳酸聚合物合成活性的氨基酸序列突变而具有乳酸聚合物和乳酸共聚物的合成活性， 乳酸聚合物和具有不同特征的共聚物，分别通过使用合成酶的突变体。

    Multi-spot metal-capped nanostructure array nucleic acid chip for diagnosis of corneal dystrophy and preparation method thereof
    6.
    发明授权
    Multi-spot metal-capped nanostructure array nucleic acid chip for diagnosis of corneal dystrophy and preparation method thereof 有权
    用于诊断角膜营养不良的多点金属封端纳米结构阵列核酸芯片及其制备方法

    公开(公告)号：US09145583B2

    公开(公告)日：2015-09-29

    申请号：US13391167

    申请日：2010-08-18

    申请人： Sang Yup Lee ,  So Young Yoo ,  Do Kyun Kim ,  Tae Jung Park ,  Jung Kuk Yun ,  Gene Lee

    发明人： Sang Yup Lee ,  So Young Yoo ,  Do Kyun Kim ,  Tae Jung Park ,  Jung Kuk Yun ,  Gene Lee

    IPC分类号： C12Q1/68 ,  C12M1/36 ,  B82Y30/00

    CPC分类号： C12Q1/6837 ,  B01J2219/00509 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00614 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6883 ,  C12Q2600/158

    摘要： A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.

    摘要翻译： 一种用于诊断角膜营养不良的多点金属帽纳米结构阵列核酸芯片，更具体地涉及能够使用LSPR（局部表面等离子共振）光学性质的多点金属封端的纳米结构阵列核酸芯片，其制备方法 ，以及用于诊断BIGH3基因突变的多点金属帽纳米结构阵列核酸芯片，其可以诊断各种角膜营养不良。 金属封端的纳米结构阵列核酸芯片可以与包括光源，检测器，分光光度计和计算机的分析装置组合，以提供基于LSPR光学性质的光学生物传感器，并且使用多点金属 用于诊断BIGH3基因突变的封闭的纳米结构阵列核酸芯片允许同时诊断作为遗传性眼部疾病的各种角膜营养不良。

    Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same
    7.
    发明授权
    Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same 有权
    具有烃生产能力的微生物变体及使用其生产烃的方法

    公开(公告)号：US08895276B2

    公开(公告)日：2014-11-25

    申请号：US13702920

    申请日：2011-06-10

    申请人： Sang Yup Lee ,  Yong Jun Choi

    发明人： Sang Yup Lee ,  Yong Jun Choi

    IPC分类号： C12P5/00 ,  C12N9/02 ,  C12N9/18 ,  C12N9/88 ,  C12N9/00 ,  C12P5/02 ,  C12N15/52 ,  C12N15/70 ,  C07K14/245

    CPC分类号： C12P5/00 ,  C07K14/245 ,  C12N9/001 ,  C12N9/18 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12P5/005 ,  C12P5/02 ,  C12P5/026

    摘要： The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.

    摘要翻译： 本发明涉及具有生产碳氢化合物（包括烷烃）的能力的微生物变异体，以及使用该方法生产烃类的方法，包括使用烷烃的烷烃，更具体地说，涉及通过引入编码转化脂肪酶的基因而获得的微生物变体 对游离脂肪酸的酰基-Acp，编码将游离脂肪酸转化为脂肪酰辅酶A的酶的基因，编码将脂肪酰基-CoA转化为脂肪醛的酶的基因和编码将脂肪醛转化为烷烃的酶的基因转化为微生物 改进以适于生产包括烷烃在内的烃类，以及使用其制备包括烷烃在内的烃类的方法。 本发明的微生物变体具有很高的潜力用于通过附加的代谢通量工程来改善菌株，因此可用于包括烷烃在内的烃的工业生产。

    MULTI-SPOT METAL-CAPPED NANOSTRUCTURE ARRAY NUCLEIC ACID CHIP FOR DIAGNOSING OF CORNEAL DYSTROPHY AND PREPARATION METHOD THEREOF PRODUCING SAME
    9.
    发明申请
    MULTI-SPOT METAL-CAPPED NANOSTRUCTURE ARRAY NUCLEIC ACID CHIP FOR DIAGNOSING OF CORNEAL DYSTROPHY AND PREPARATION METHOD THEREOF PRODUCING SAME 有权
    多点金属填充纳米结构阵列核苷酸芯片，用于诊断角膜晶状体及其制备方法

    公开(公告)号：US20140243222A1

    公开(公告)日：2014-08-28

    申请号：US13391167

    申请日：2010-08-18

    申请人： Sang Yup Lee ,  So Young Yoo ,  Do Kyun Kim ,  Tae Jung Park ,  Jung Kuk Yun ,  Gene Lee

    发明人： Sang Yup Lee ,  So Young Yoo ,  Do Kyun Kim ,  Tae Jung Park ,  Jung Kuk Yun ,  Gene Lee

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  B01J2219/00509 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00614 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6883 ,  C12Q2600/158

    摘要： A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.

    摘要翻译： 一种用于诊断角膜营养不良的多点金属帽纳米结构阵列核酸芯片，更具体地涉及能够使用LSPR（局部表面等离子共振）光学性质的多点金属封端的纳米结构阵列核酸芯片，其制备方法 ，以及用于诊断BIGH3基因突变的多点金属帽纳米结构阵列核酸芯片，其可以诊断各种角膜营养不良。 金属封端的纳米结构阵列核酸芯片可以与包括光源，检测器，分光光度计和计算机的分析装置组合，以提供基于LSPR光学性质的光学生物传感器，并且使用多点金属 用于诊断BIGH3基因突变的封闭的纳米结构阵列核酸芯片允许同时诊断作为遗传性眼部疾病的各种角膜营养不良。

    RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME
    10.
    发明申请
    RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME 有权
    具有生产丁醇的能力提高的重组微生物和使用其的丁醇的生产方法

    公开(公告)号：US20130017588A1

    公开(公告)日：2013-01-17

    申请号：US13497743

    申请日：2010-09-21

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    IPC分类号： C12N1/21 ,  C12P7/16 ,  C12N15/01

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

    摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

    摘要翻译： 本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。 更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。 在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。 通过操作微生物的代谢通过获得的重组微生物能够高效地选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。