公开(公告)号：US20220111389A1

公开(公告)日：2022-04-14

申请号：US17515959

申请日：2021-11-01

申请人： ALERE SAN DIEGO, INC. ,  ALERE SWITZERLAND GMBH

发明人： Daniel Nicholas HAWORTH ,  John Paul PALMER-FELGATE

IPC分类号： B01L3/00 ,  F04B9/14 ,  G01N30/20 ,  B01J20/281 ,  F04B53/10 ,  B01L3/02 ,  F04B13/00 ,  G01N1/14 ,  G01N1/40 ,  F04B9/12 ,  G01N30/14 ,  B01D15/12 ,  B01D15/34 ,  C12Q1/6844 ,  G01N30/02 ,  G01N30/00

摘要： A manually actuated chromatography device comprising a chamber for receiving a liquid sample, a pump with a metering valve, and a chromatography element, wherein the pump moves a predetermined volume of liquid from the sample chamber to the chromatography element.

公开(公告)号：US10329603B2

公开(公告)日：2019-06-25

申请号：US15496756

申请日：2017-04-25

申请人： Alere San Diego Inc.

发明人： Olaf Piepenburg ,  Colin H. Williams ,  Niall A. Armes ,  Derek L. Stemple

IPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12N9/12 ,  B01L7/00 ,  C12Q1/6818 ,  G01N33/53

摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.

公开(公告)号：US10329602B2

公开(公告)日：2019-06-25

申请号：US15395446

申请日：2016-12-30

申请人： Alere San Diego, Inc.

发明人： Olaf Piepenburg ,  Colin H. Williams ,  Niall A. Armes ,  Derek L. Stemple

IPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/70

摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundres of megabases in length.

公开(公告)号：US20180353597A1

公开(公告)日：2018-12-13

申请号：US16105299

申请日：2018-08-20

申请人： ALERE SAN DIEGO, INC.

发明人： Mariusz BANASZCZYK ,  Normand HÉBERT ,  Neil STOWE

IPC分类号： A61K39/385 ,  A61K39/39 ,  C08L25/06 ,  A61K39/00 ,  G01N33/94 ,  C07D489/02 ,  C07K16/06 ,  C07K16/44 ,  C07D489/04 ,  C07D489/00 ,  C07F9/6561

CPC分类号： A61K39/385 ,  A61K39/0013 ,  A61K39/39 ,  A61K2039/6081 ,  A61K2039/627 ,  C07D489/00 ,  C07D489/02 ,  C07D489/04 ,  C07F9/6561 ,  C07K16/06 ,  C07K16/44 ,  C07K2317/33 ,  C08L25/06 ,  G01N33/9486

摘要： The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.

公开(公告)号：US10052379B2

公开(公告)日：2018-08-21

申请号：US15640781

申请日：2017-07-03

申请人： ALERE SAN DIEGO, INC.

发明人： Mariusz Banaszczyk ,  Normand Hébert ,  Neil Stowe

IPC分类号： A61K39/385 ,  C07D489/02 ,  C07F9/6561 ,  C07D489/00 ,  C07D489/04 ,  C07K16/44 ,  C07K16/06 ,  A61K39/39 ,  G01N33/94 ,  A61K39/00 ,  C08L25/06

CPC分类号： A61K39/385 ,  A61K39/0013 ,  A61K39/39 ,  A61K2039/6081 ,  A61K2039/627 ,  C07D489/00 ,  C07D489/02 ,  C07D489/04 ,  C07F9/6561 ,  C07K16/06 ,  C07K16/44 ,  C07K2317/33 ,  C08L25/06 ,  G01N33/9486

摘要： The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.

公开(公告)号：US09694069B2

公开(公告)日：2017-07-04

申请号：US14775644

申请日：2014-03-14

申请人： ALERE SAN DIEGO, INC.

发明人： Mariusz Banaszczyk ,  Normand Hébert ,  Neil Stowe

IPC分类号： A61K39/385 ,  C07D489/02 ,  C07D489/00 ,  C07D489/04 ,  C07F9/6561 ,  A61K39/00 ,  A61K39/39 ,  C07K16/06 ,  C07K16/44 ,  C08L25/06 ,  G01N33/94

CPC分类号： A61K39/385 ,  A61K39/0013 ,  A61K39/39 ,  A61K2039/6081 ,  A61K2039/627 ,  C07D489/00 ,  C07D489/02 ,  C07D489/04 ,  C07F9/6561 ,  C07K16/06 ,  C07K16/44 ,  C07K2317/33 ,  C08L25/06 ,  G01N33/9486

摘要： The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.

公开(公告)号：US09663820B2

公开(公告)日：2017-05-30

申请号：US14989499

申请日：2016-01-06

申请人： Alere San Diego Inc.

发明人： Olaf Piepenburg ,  Colin H. Williams ,  Niall A. Armes ,  Derek L. Stemple

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12N9/12 ,  B01L7/00 ,  G01N33/53

CPC分类号： C12Q1/6844 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/18 ,  C12N9/1252 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Y207/07007 ,  G01N33/5308 ,  C12Q2531/113 ,  C12Q2522/101 ,  C12Q2521/507 ,  C12Q2527/125

摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.

公开(公告)号：US09316640B2

公开(公告)日：2016-04-19

申请号：US14355843

申请日：2012-11-09

申请人： Alere San Diego, Inc.

发明人： Ralf Ehricht ,  Stefan Monecke ,  John J. Rejman ,  Joseph Buechler

IPC分类号： G01N33/569 ,  G01N33/53 ,  C07K16/12

CPC分类号： G01N33/56938 ,  C07K16/1271 ,  G01N33/53

摘要： The present invention provides methods and devices for detecting the presence of biomolecules in a biological sample, such as PVL, PBP2a and SPA.

摘要翻译： 本发明提供用于检测生物样品如PVL，PBP2a和SPA中存在生物分子的方法和装置。

公开(公告)号：US08809021B2

公开(公告)日：2014-08-19

申请号：US13441411

申请日：2012-04-06

申请人： Niall A. Armes ,  Olaf Piepenburg ,  Catherine Jean Greenwood

发明人： Niall A. Armes ,  Olaf Piepenburg ,  Catherine Jean Greenwood

IPC分类号： C12P19/34

CPC分类号： C12Q1/6851 ,  C12Q1/6844 ,  C12Q1/689 ,  C12Q2600/158 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6432 ,  Y02A50/53 ,  C12Q2521/507 ,  C12Q2522/101 ,  C12Q2527/137 ,  C12Q2565/629 ,  C12Q2563/155 ,  C12Q2563/159

摘要： A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.

摘要翻译： 一种方法包括提供包含重组酶，单链结合蛋白和一种或多种寡核苷酸的混合物; 并检测反应混合物中的颗粒。

公开(公告)号：US08795975B2

公开(公告)日：2014-08-05

申请号：US13990393

申请日：2011-11-25

申请人： William D. Arnold ,  Christelle Jost ,  Brian Noland ,  Jonathan Gary ,  Joseph Buechler ,  Vance Wong ,  Scott Harold Rongey ,  Uday Kumar Veeramallu ,  Kelline Marie Rodems

发明人： William D. Arnold ,  Christelle Jost ,  Brian Noland ,  Jonathan Gary ,  Joseph Buechler ,  Vance Wong ,  Scott Harold Rongey ,  Uday Kumar Veeramallu ,  Kelline Marie Rodems

IPC分类号： G01N31/00 ,  G01N33/53

CPC分类号： G01N33/6893 ,  G01N2800/325 ,  G01N2800/60

摘要： The present invention relates in part to diagnosing the occurrence of heart failure, particularly in subjects who exhibit a normal body fluid level of a natriuretic peptide. The present invention further relates in part to assigning an outcome risk (e.g., worsening cardiac function or a mortality risk, a risk of rehospitalization) to a subject. The methods comprise performing one or more assays that detect one or more biomarkers selected from the group consisting of WAP4C, ESAM, LTBR, Mesothelin, and Syndecan-1 performed on a body fluid sample obtained from a subject, and assigning diagnosis or risk based, at least in part, on the result(s) obtained thereby.

摘要翻译： 本发明部分地涉及诊断心力衰竭的发生，特别是在表现出利钠肽的正常体液水平的受试者中。 本发明还部分地涉及将受试者的结局风险（例如恶化心脏功能或死亡风险，再住院风险）分配给患者。 所述方法包括进行一种或多种测定，所述测定法检测在从受试者获得的体液样品上进行的选自由WAP4C，ESAM，LTBR，间皮素和Syndecan-1组成的组中的一种或多种生物标志物，以及分配诊断或风险， 至少部分地取决于由此获得的结果。