公开(公告)号：US09370774B2

公开(公告)日：2016-06-21

申请号：US12682555

申请日：2008-10-13

申请人： Kishore Krishna Kumar ,  Raviprakash Jayaraman ,  Sankaranand Kaipa Narasimha ,  Renjith Mahiladevi Radhakrishnan ,  Sathyadeep Viswanathan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Manjula Jagannath ,  Shilpa Chennakrishnaiah ,  Sudip Mondal ,  Venkatakrishnan Venkataraman

发明人： Kishore Krishna Kumar ,  Raviprakash Jayaraman ,  Sankaranand Kaipa Narasimha ,  Renjith Mahiladevi Radhakrishnan ,  Sathyadeep Viswanathan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Manjula Jagannath ,  Shilpa Chennakrishnaiah ,  Sudip Mondal ,  Venkatakrishnan Venkataraman

IPC分类号： C12M1/38 ,  B01L7/00 ,  B01L3/00

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L2300/0627 ,  B01L2300/0851 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/1805

摘要： Instant invention is about a hand held micro PCR device comprising a LTCC micro PCR chip comprising a heater, a reaction chamber to load a sample. It also comprises a heater control to regulate the heater on basis of input received from a temperature sensor. It further has an optical system having an optical fiber to detect a fluorescence signal from the sample, and at least one communication interface to interact with other device(s).

摘要翻译： 本发明涉及一种手持式微PCR装置，其包含LTCC微PCR芯片，其包括加热器，负载样品的反应室。 它还包括加热器控制，以根据从温度传感器接收的输入来调节加热器。 它还具有光学系统，其具有用于检测来自样品的荧光信号的光纤，以及至少一个通信接口以与其他设备相互作用。

公开(公告)号：US09238674B2

公开(公告)日：2016-01-19

申请号：US13973102

申请日：2013-08-22

申请人： Bigtec Private Limited

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07K1/18 ,  C07K1/22 ,  C12N15/10 ,  C12N15/81 ,  C12N1/18 ,  C12N1/19 ,  C07K14/62 ,  C07K1/00

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus Pichia. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.

摘要翻译： 在一个方面，方法涉及重组DNA治疗领域。 方法可涉及生物信息学设计，人前体肽编码序列的人胰岛素前体的合成，将人工基因克隆到表达载体中，并在生物体中表达，例如选自毕赤酵母属（Pichia）的生物。 在另一方面，方法可以包括用于获得蛋白质前体分子和随后将蛋白质前体分子转化为功能性蛋白质的下游处理。

公开(公告)号：US10227633B2

公开(公告)日：2019-03-12

申请号：US15288781

申请日：2016-10-07

申请人： BIGTEC PRIVATE LIMITED

发明人： Manoj Mulakkapurath Narayanan ,  Chandrasekhar Bhaskaran Nair

IPC分类号： C12Q1/686 ,  C12Q1/6806 ,  B01L7/00 ,  B01L3/00

摘要： The present disclosure provides a portable device for purifying biological sample. The device comprises a housing and a cartridge holder accommodated in the housing for holding at least one cartridge configured to purify the biological sample. At least one pump is positioned in the housing which is fluidly connectable to the cartridge to circulate the sample and one or more fluids through the cartridge. Further, at least one heating element is configured to heat a matrix chamber in the cartridge. At least one actuator is disposed in the housing to actuate one or more valves to selectively route the biological sample and the one or more fluids in the cartridge. A control unit is interfaced with the actuator, the heating element and the pump and is configured to regulate operation of the actuator, the pump and the heating element during purification of the biological sample.

公开(公告)号：US08986976B2

公开(公告)日：2015-03-24

申请号：US13520685

申请日：2011-01-06

申请人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12N1/08 ,  C07H1/08 ,  C12N15/10

CPC分类号： C07H1/08 ,  C12N15/10 ,  C12N15/1006

摘要： The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.

摘要翻译： 本公开提供了使用棉花从固体或液体样品分离天然和人造核酸如脱氧核糖核酸（DNA），核糖核酸（RNA）和肽核酸（PNA）的方法。 包装的棉花使得含有核酸的溶液通过它，并且溶液中的核酸在最适于结合的培养基中与棉花结合。 核酸以这样的方式结合棉花，使得结合的核酸可以经受含有水的液体的多次洗涤，并在水性缓冲液中洗脱，其中洗脱的核酸可以直接用于使用PCR或任何其它的扩增 生物化学或分子生物学的需要。

公开(公告)号：US20130130235A1

公开(公告)日：2013-05-23

申请号：US13812863

申请日：2011-07-15

申请人： Manjula Jagannath ,  Manoj Mulakkapurath Narayanan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Manjula Jagannath ,  Manoj Mulakkapurath Narayanan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12Q1/70

CPC分类号： C12Q1/701

摘要： The present disclosure gives description of a method used for the detection and quantification of dengue viral infection caused by dengue virus using nucleic acids isolated from blood, plasma or serum samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR. The instant disclosure also provides for primers, probes, PCR Reaction mixture and kit thereof.

摘要翻译： 本公开描述了使用通过使用寡核苷酸探针从血液，血浆或血清样品分离的核酸来检测和定量由登革热病毒引起的登革热病毒感染的方法的描述。 这里用于检测的方法是通过实时PCR。 本公开还提供了引物，探针，PCR反应混合物及其试剂盒。

公开(公告)号：US20100240044A1

公开(公告)日：2010-09-23

申请号：US12682581

申请日：2008-10-13

申请人： Kishore Krishna Kumar ,  Raviprakash Jayaraman ,  Sankaranand Kaipa Narasimha ,  Renjith Mahiladevi Radhakrishnan ,  Sathyadeep Viswanathan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Manjula Jagannath ,  Shilpa Chennakrishnaiah

发明人： Kishore Krishna Kumar ,  Raviprakash Jayaraman ,  Sankaranand Kaipa Narasimha ,  Renjith Mahiladevi Radhakrishnan ,  Sathyadeep Viswanathan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Manjula Jagannath ,  Shilpa Chennakrishnaiah

IPC分类号： C12Q1/68 ,  C12M1/34 ,  H05K3/46

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L2300/0627 ,  B01L2300/0851 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/1805

摘要： Instant invention is about a micro chip comprising plurality of layers of LTCC wherein a reaction chamber is formed in plurality of top layers to load samples. A heater embedded in at least one of the layers below the reaction chamber and a temperature sensor is embedded in at least one of the layers between the heater and the reaction chamber for analyzing the sample. The temperature sensor can be placed outside the chip to measure the chip temperature.

摘要翻译： 即时发明是关于包括多层LTCC的微芯片，其中反应室形成在多个顶层中以加载样品。 嵌入在反应室下面的至少一个层的加热器和温度传感器被嵌入在加热器和反应室之间的至少一个层中，用于分析样品。 温度传感器可以放置在芯片外部以测量芯片温度。

公开(公告)号：US09309281B2

公开(公告)日：2016-04-12

申请号：US13973130

申请日：2013-08-22

申请人： Bigtec Private Limited

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： A61K38/28 ,  C07K14/62 ,  C07K1/18

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus Pichia. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.

摘要翻译： 在一个方面，方法涉及重组DNA治疗领域。 方法可涉及生物信息学设计，人前体肽编码序列的人胰岛素前体的合成，将人工基因克隆到表达载体中，并在生物体中表达，例如选自毕赤酵母属（Pichia）的生物。 在另一方面，方法可以包括用于获得蛋白质前体分子和随后将蛋白质前体分子转化为功能性蛋白质的下游处理。

公开(公告)号：US09044754B2

公开(公告)日：2015-06-02

申请号：US12682581

申请日：2008-10-13

申请人： Kishore Krishna Kumar ,  Raviprakash Jayaraman ,  Sankaranand Kaipa Narasimha ,  Renjith Mahiladevi Radhakrishnan ,  Sathyadeep Viswanathan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Manjula Jagannath ,  Shilpa Chennakrishnaiah

发明人： Kishore Krishna Kumar ,  Raviprakash Jayaraman ,  Sankaranand Kaipa Narasimha ,  Renjith Mahiladevi Radhakrishnan ,  Sathyadeep Viswanathan ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Manjula Jagannath ,  Shilpa Chennakrishnaiah

IPC分类号： C12Q1/68 ,  B01L7/00 ,  C04B35/00 ,  B01L3/00

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L2300/0627 ,  B01L2300/0851 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/1805

摘要： Instant invention is about a micro chip comprising plurality of layers of LTCC wherein a reaction chamber is formed in plurality of top layers to load samples. A heater embedded in at least one of the layers below the reaction chamber and a temperature sensor is embedded in at least one of the layers between the heater and the reaction chamber for analyzing the sample. The temperature sensor can be placed outside the chip to measure the chip temperature.

摘要翻译： 即时发明是关于包括多层LTCC的微芯片，其中反应室形成在多个顶层中以加载样品。 嵌入在反应室下面的至少一个层的加热器和温度传感器被嵌入在加热器和反应室之间的至少一个层中，用于分析样品。 温度传感器可以放置在芯片外部以测量芯片温度。

公开(公告)号：US08575322B2

公开(公告)日：2013-11-05

申请号：US13256028

申请日：2010-03-12

申请人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07H21/04 ,  C12N15/17

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

摘要翻译： 本公开涉及重组DNA治疗领域。 它包括生物信息学设计，人胰岛素前体的人造基因的合成，包括前导肽编码序列，表达载体中的克隆和生物体中优选巴斯德毕赤酵母表达。 本公开还涉及用于获得蛋白质前体分子和随后将前体分子转化成功能性蛋白质的下游处理方法。

公开(公告)号：US20110306037A1

公开(公告)日：2011-12-15

申请号：US13201215

申请日：2010-01-27

申请人： Manjula Jaggannath ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Manjula Jaggannath ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12Q1/70 ,  C07H21/04

CPC分类号： C12Q1/706 ,  C12Q2561/113

摘要： The present disclosure provides a method for the detection and quantification of Hepatitis B Virus. It discloses oligonucleotide probes set forth in SEQ ID Nos. 1 and 2 for detection of Hepatitis B Virus along with respective primers [sense and antisense] set forth in SEQ ID Nos. 3, 4, 5 and 6. It also provides a PCR reaction mixture for detection of Hepatitis B Virus and a kit for detection of HBV comprising said mixture along with an instruction package.

摘要翻译： 本公开提供了用于检测和定量乙型肝炎病毒的方法。 它公开了用于检测乙型肝炎病毒的SEQ ID No.1和2所示的寡核苷酸探针以及SEQ ID No.3,4,5和6中列出的相应引物[有义和反义]。还提供PCR反应 用于检测乙型肝炎病毒的混合物和用于检测HBV的试剂盒，包括所述混合物以及指令包。