公开(公告)号：US6124126A

公开(公告)日：2000-09-26

申请号：US178610

申请日：1998-10-26

申请人： Yoshifumi Ito ,  Seung-Moon Park ,  Truong Nam Hai

发明人： Yoshifumi Ito ,  Seung-Moon Park ,  Truong Nam Hai

IPC分类号： A01H5/00 ,  C12N1/19 ,  C12N1/21 ,  C12N9/24 ,  C12N9/42 ,  C12N15/09 ,  C12R1/19 ,  C12R1/84

CPC分类号： C12Y302/01014 ,  C12N9/2442

摘要： The present invention provides a complementary DNA for a rice chitinase having a lytic activity against moulds and a bacteria, a complementary DNA for a rice chitinase encoding a protein comprising an amino acid sequence described in SEQ ID NO: 1 in SEQUENCE LISTING, a plasmid vector containing said complementary DNA and a transformant having said plasmid vector. The present invention enables realization of a possibility of developing a recombinant crop plant having an acquired resistance against a wide range of pathogenic microorganisms by identifying a complementary DNA encoding a novel chitinase capable of lysing cell walls of both of moulds and bacteria and introducing the sequence thereof into a plant.

摘要翻译： 本发明提供了具有针对霉菌和细菌的裂解活性的水稻几丁质酶的互补DNA，用于编码包含序列表中SEQ ID NO：1所示氨基酸序列的蛋白质的水稻几丁质酶的互补DNA，质粒载体 含有所述互补DNA和具有所述质粒载体的转化体。 本发明可以实现通过鉴定编码能够裂解霉菌和细菌的细胞壁的新型几丁质酶的互补DNA并引入其序列来开发具有获得的抗广泛病原微生物抗性的重组作物的可能性 进入植物。

公开(公告)号：US5302699A

公开(公告)日：1994-04-12

申请号：US7754

申请日：1993-01-22

申请人： Yukio Kawamura ,  Akihiro Morita ,  Makoto Tomatsu ,  Masaru Ishikawa

发明人： Yukio Kawamura ,  Akihiro Morita ,  Makoto Tomatsu ,  Masaru Ishikawa

IPC分类号： A61K36/07 ,  A61K38/00 ,  A61P35/00 ,  C07K1/14 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K14/37 ,  C07K14/375 ,  C07K14/41 ,  C07K14/415 ,  A61K35/80 ,  C07K3/22

CPC分类号： C07K14/37 ,  A61K38/00

摘要： Disclosed is an antitumorigenic protein derived from fruit bodies of matsutake mushrooms (Tricholoma matsutake). Also disclosed is a method of preparing the antitumorigenic protein comprising extracting fruit bodies of matsutake mushrooms with water followed by subjecting the resulting extract to purification composed of combination of the following three purifying steps in any desired order:(1) a step of purifying the protein by molecular sieve chromatography in a gel permeation column;(2) a step of bringing the extract in contact with an anion exchange resin so that the protein is adsorbed to the resin followed by eluting the protein from the column of the resin with an eluent; and(3) a step of bringing the extract in contact with a hydrophobic chromatographic resin so that the protein is adsorbed to the resin followed by eluting the protein from the column of the resin with an eluent.Further disclosed is an antitumorigenic composition to human and animal epithelial cancer, which contains the protein as an active component.

摘要翻译： 本发明公开了一种衍生自松果子（Tricholoma matsutake）果实的抗发霉蛋白质。 还公开了制备抗发霉蛋白质的方法，其包括用水提取松果子的果实，然后以任何所需的顺序对得到的提取物进行以下三个纯化步骤的组合的纯化：（1）纯化蛋白质的步骤 通过分子筛色谱法在凝胶渗透柱中; （2）使提取物与阴离子交换树脂接触以使蛋白质吸附到树脂上的步骤，然后用洗脱液从树脂柱中洗脱出蛋白质; 和（3）使提取物与疏水性色谱树脂接触以使蛋白质吸附到树脂上的步骤，然后用洗脱液从树脂柱洗脱蛋白质。 进一步公开的是含有作为活性成分的蛋白质的人和动物上皮癌的抗致癌组合物。

公开(公告)号：US4939091A

公开(公告)日：1990-07-03

申请号：US88858

申请日：1987-08-24

申请人： Takashi Sasaki ,  Takafumi Kasumi ,  Naoya Kubo ,  Keiji Kainuma ,  Katsuo Wako ,  Hiroaki Ishizuka ,  Gaku Kawaguchi ,  Tsunero Oda

发明人： Takashi Sasaki ,  Takafumi Kasumi ,  Naoya Kubo ,  Keiji Kainuma ,  Katsuo Wako ,  Hiroaki Ishizuka ,  Gaku Kawaguchi ,  Tsunero Oda

IPC分类号： C12N1/02 ,  C12P7/18

CPC分类号： C12R1/645 ,  C12P7/18 ,  Y10S435/911

摘要： Biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof according to the present invention show the properties of forming and accumulating erythritol in a culture solution, when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are useful for the preparation of erythritol by fermentation of sugars. A method for preparing erythritol by fermentation of sugars according to the present invention comprises inoculating microorganism selected from the group consisting of Aureobasidium sp. SN-124A strain (FERM BP-1429) and mutants thereof on a liquid culture medium of pH 4 to 9 containing an assimilable carbon source and an assimilable nitrogen source, and aerobically culturing them at a temperature of 30.degree. to 38.degree. C. to form and accumulate erythritol in said culture medium for collection.

摘要翻译： 生物纯培养的Aureobasidium sp。 根据本发明的SN-124A菌株（FERM BP-1429）及其人造突变体，当在含有可同化的碳源和可同化的氮源的液体培养基上进行有氧培养时，显示培养液中形成和积累赤藓糖醇的性质 ，可用于通过发酵糖制备赤藓糖醇。 通过根据本发明的糖的发酵制备赤藓糖醇的方法包括接种微生物，其选自由Aureobasidium sp。 SN-124A菌株（FERM BP-1429）及其在pH4〜9的液体培养基上的突变体，其含有可同化的碳源和可吸收的氮源，并在30℃至38℃的温度下进行有氧培养， 在所述培养基中形成并积累赤藓糖醇用于收集。

公开(公告)号：US06960458B2

公开(公告)日：2005-11-01

申请号：US10040416

申请日：2002-01-09

申请人： Tetuya Ookura ,  Takafumi Kasumi ,  Eiji Asaba

发明人： Tetuya Ookura ,  Takafumi Kasumi ,  Eiji Asaba

IPC分类号： C12N9/02 ,  C12P7/18 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P7/02

CPC分类号： C12N9/0008 ,  C12N9/0036 ,  C12P7/18

摘要： Protein having an erythrose reductase activity; a DNA encoding the protein; a cell to which a DNA has been transferred in a manner such that the DNA is capable of expressing an erythrose reductases type III, II or I the DNA encodes; and a method for producing erythritol, comprising acting the erythrose reductases type III, II or I or a cell to which erythrose reductases type III, II or I has been transferred in a manner capable of expressing the erythrose reductases type III, II or I on D-erythrose and harvesting the produced erythritol.

摘要翻译： 具有红葡萄糖还原酶活性的蛋白质; 编码蛋白质的DNA; 以使得DNA能够表达DNA编码的III型，II型或I型红血球还原酶的方式转移DNA的细胞; 以及制备赤藓糖醇的方法，其包括以III，II或I型赤藓红还原酶或以能够将III型，II型或I型赤藓糖还原酶表达的方式转移到其中的III型，II型或I型赤藓红还原酶的细胞， D-赤藓糖并收获所生产的赤藓糖醇。

公开(公告)号：US6107471A

公开(公告)日：2000-08-22

申请号：US410028

申请日：1999-10-01

申请人： Masaomi Arahira ,  Chikafusa Fukazawa

发明人： Masaomi Arahira ,  Chikafusa Fukazawa

IPC分类号： C12N15/09 ,  C12N9/50 ,  C12N9/64 ,  C12N15/00 ,  C12N15/29 ,  C12N15/57 ,  C12R1/91

CPC分类号： C12N9/6478

摘要： Disclosed are a cDNA encoding a plant-derived, asparagine residue-specific endoprotease having an amino sequence described in SEQ ID NO: 1 in the sequence list and a gene encoding a plant-derived, asparagine residue-specific endoprotease having an amino sequence described in SEQ ID NO: 2 in the sequence listing. The enzymes enable production of asparagine residue-specific endoprotease in large amounts.

摘要翻译： 公开了编码具有序列表中SEQ ID NO：1所示的氨基序列的植物来源的天冬酰胺残基特异性内切蛋白酶的cDNA，以及编码具有氨基酸序列的植物来源的天冬酰胺残基特异性内切蛋白酶的基因 序列表中的SEQ ID NO：2。 这些酶能够大量生产天冬酰胺残基特异性内切蛋白酶。

公开(公告)号：US5955320A

公开(公告)日：1999-09-21

申请号：US900820

申请日：1997-07-25

申请人： Ken Tokuyasu ,  Hiroshi Ono ,  Mayumi Kameyama ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

发明人： Ken Tokuyasu ,  Hiroshi Ono ,  Mayumi Kameyama ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

IPC分类号： C07H13/06 ,  C07H15/12 ,  C12P19/26 ,  C07H3/04 ,  C12P19/12

CPC分类号： C07H15/12 ,  C12P19/26

摘要： Di-N-acetyl-D-chitobiose, which is easily available as a chitin decomposate or a chemical reagent, is reacted with a microorganisms-derived deacetylase to produce 2-acetylamino-4-O-(2-amino-2-deoxy-.beta.-D-glucopyranosyl)-2-deoxy-D-glucose of formula (1). ##STR1## The method is quantitative and efficiently produces the compound (1). The compound (1) and its salts are usable as substrates for measuring enzymatic activities, and also as starting substances in the production of various food materials and in the field of glyco-chain engineering.

摘要翻译： 作为几丁质分解物或化学试剂容易获得的二-N-乙酰基-D-壳寡糖与微生物衍生的脱乙酰酶反应以产生2-乙酰氨基-4-O-（2-氨基-2-脱氧 - β-D-吡喃葡萄糖基）-2-脱氧-D-葡萄糖。 该方法是定量和有效地产生化合物（1）。 化合物（1）及其盐可用作测定酶活性的底物，也可用作生产各种食品的起始物质和糖链工程领域。

公开(公告)号：US4908311A

公开(公告)日：1990-03-13

申请号：US236555

申请日：1988-08-25

申请人： Takashi Sasaki ,  Hajime Taniguchi ,  Keiji Kainuma

发明人： Takashi Sasaki ,  Hajime Taniguchi ,  Keiji Kainuma

IPC分类号： C12P19/14 ,  C08B37/00 ,  C12N9/42 ,  C12P19/04 ,  C12R1/01

CPC分类号： C12P19/04 ,  C12N9/2445 ,  C12Y302/01021

摘要： A process for enzymatic preparation of cellooligosaccharide from a cellulose-base substance by the action of cellulase produced by an microorganism belonging to the Genus Cellvibrio, wherein an ultrafiltration reactor is used in combination, so that production inhibition can be removed and cellooligosaccharide can be formed and accumulated. The present process permits preparation of cellobiose in high yield and is very advantageous from an industrial standpoint.

摘要翻译： 通过由属于细菌属属微生物的微生物的纤维素酶的作用从纤维素基物质中酶制备纤维寡糖的方法，其中组合使用超滤反应器，从而可以除去生产抑制并形成低聚果糖; 积累。 本方法允许以高产率制备纤维二糖，并且从工业观点来看是非常有利的。

公开(公告)号：US4871840A

公开(公告)日：1989-10-03

申请号：US151805

申请日：1988-02-03

申请人： Shoichi Kobayashi ,  Masaomi Arahira

发明人： Shoichi Kobayashi ,  Masaomi Arahira

IPC分类号： A23L1/03 ,  A23L1/308 ,  A61K8/72 ,  A61K8/73 ,  A61K47/40 ,  C08B37/16 ,  C12N1/38 ,  C12P19/16

CPC分类号： C08B37/0012 ,  C12P19/16

摘要： Different from conventional heterogeneous multiple-branched cyclodextrins having branches derived from the same kind of saccharide molecule, the inventive heterogeneous multiple-branched cyclodextrin has branches derived from different kinds of saccharides of, one, glucose and, the other, a maltooligosaccharide. The inventive heterogeneous multiple-branched cyclodextrins are more stable than conventional ones and useful as an ingredient of medical, foodstuff and cosmetic preparations. The heterogeneous multiple-branched cyclodextrin can be prepared from a mixture of a maltooligosaccharide and a glucosyl cyclodextrin by the reverse action of debranching enzyme.

公开(公告)号：US6087147A

公开(公告)日：2000-07-11

申请号：US17706

申请日：1998-02-05

申请人： Yoshifumi Ito

发明人： Yoshifumi Ito

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/28 ,  C12N15/00 ,  C12P19/14 ,  C12R1/19 ,  C12R1/38 ,  C12W9/28 ,  C07H21/04 ,  C12N1/20 ,  C12W1/20

CPC分类号： C12N9/2417 ,  C12P19/14

摘要： A modified enzyme having a reduced maltopentaose decomposing activity and improvements in practical usability was provided by a gene coding for .alpha.-amylase highly producing maltopentaose, the .alpha.-amylase comprising an amino acid sequence where an amino acid residue at 57- or 139-position has been substituted in the amino acid sequence of maltopentaose-forming amylase derived from Pseudomonas sp. KO-8940 (Shida, O. et al., Biosci. Biotech. Biochem. Vol. 56, 76-80 (1992)).

摘要翻译： 通过编码高产麦芽糖的α-淀粉酶的基因提供了具有降低的麦芽五糖分解活性和实用可用性改进的修饰酶，所述α-淀粉酶包含其中57位或139位氨基酸残基具有氨基酸残基的氨基酸序列 在衍生自假单胞菌属（Pseudomonas sp。）的麦芽五糖形成淀粉酶的氨基酸序列中被取代。 KO-8940（Shida，O.et al。，Biosci.Biotech.Biochem.Vol。56,76-80（1992））。

公开(公告)号：US6050178A

公开(公告)日：2000-04-18

申请号：US260418

申请日：1999-03-01

申请人： Kunihiko Uemura

发明人： Kunihiko Uemura

IPC分类号： A23L3/005 ,  A23L3/01 ,  A23L3/015 ,  A23L3/32 ,  A61L2/02 ,  C02F1/48

CPC分类号： A23L3/0155 ,  A23L3/005 ,  A23L3/01 ,  A23L3/32 ,  Y10S99/14

摘要： A continuous liquid pasteurizing apparatus and method for effectively pasteurizing microbes or bacteria having heat-resistance and/or pressure-resistance, such as sporangium, with low temperature or by maintaining a high temperature for a short time, wherein an electricity conducting unit (9) for pasteurizing the liquid is provided within a pressurized container (1), and an upper end portion of the electricity conducting unit(9)is connected to a supply opening (3)for liquid, while a lower end portion thereof is connected to a reservoir portion(10)which is cooled by a cooling mechanism involving heat exchange with cooling water(7). The thus-pasteurized liquid is reserved in a reservoir portion (10)and is taken out from an outlet opening(4)through a conduit (11)outside the container(1).

摘要翻译： 一种连续液体巴氏杀菌装置和方法，其具有低温或短时间内保持高温的具有耐热性和/或耐压性的微生物或细菌如孢子囊，其中导电单元（9） 用于巴氏消毒的液体设置在加压容器（1）内，并且导电单元（9）的上端部连接到用于液体的供应开口（3），而其下端部连接到储存器 部分（10）由与冷却水（7）进行热交换的冷却机构冷却。 这样巴氏灭菌的液体被保留在储存器部分（10）中，并且通过容器（1）外部的导管（11）从出口开口（4）中取出。