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73 条记录 (耗时 0.072 秒)

    Automated process for preparing treated microparticles especially blood cell components
    1.
    发明授权
    Automated process for preparing treated microparticles especially blood cell components 失效
    用于制备经处理的微粒，特别是血细胞成分的自动化方法

    公开(公告)号：US5681709A

    公开(公告)日：1997-10-28

    申请号：US375082

    申请日：1995-01-17

    申请人： Dennis Mochnal ,  Janice Jakway ,  Gilbert J. Quinton

    发明人： Dennis Mochnal ,  Janice Jakway ,  Gilbert J. Quinton

    IPC分类号： A61K35/18 ,  C12S3/22 ,  G01N33/50 ,  G01N33/543 ,  G01N33/554 ,  G01N33/80 ,  G01N33/49

    CPC分类号： G01N33/54313 ,  G01N33/5005 ,  G01N33/554 ,  Y10T436/25

    摘要： A method for preparing treated naturally occurring or synthetic particles using a blood cell processor is provided. The method comprises adjusting the blood cell processor to allow a dispersion of such particles to come into contact with a treatment material in an amount and for a period of time sufficient to modify all or a portion of the surface of such particles. In a preferred embodiment, the method comprises washing collected red blood cells; separating a selected volume of said washed red blood cells; and treating one portion of said separated washed cells with a selected proteolytic enzyme, to prepare a red blood cell reagent.

    摘要翻译： 提供了使用血细胞处理器制备治疗的天然存在或合成颗粒的方法。 该方法包括调整血液细胞处理器以允许这样的颗粒的分散与处理材料接触，其量足以修饰这些颗粒的全部或一部分表面。 在优选的实施方案中，该方法包括洗涤收集的红细胞; 分离所选洗涤的红细胞的体积; 并用选择的蛋白水解酶处理一部分所述分离的洗涤细胞，以制备红细胞试剂。

    Method of preparing nucleotide probes using a bridging complement
    2.
    发明授权
    Method of preparing nucleotide probes using a bridging complement 失效
    使用桥连补体制备核苷酸探针的方法

    公开(公告)号：US5538872A

    公开(公告)日：1996-07-23

    申请号：US302332

    申请日：1994-09-08

    申请人： Chander Bahl ,  Leopoldo Mendoza ,  Frank J. McMahon

    发明人： Chander Bahl ,  Leopoldo Mendoza ,  Frank J. McMahon

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/6813 ,  C12Q1/6816

    摘要： The present invention provides labeled nucleotide probes and a procedure for synthesis of the labeled nucleotide probe by a coupling or joinder of two components of the probe, namely a target recognition sequence moiety and a signal generating moiety, through the use of a bridging complement that holds the two moieties in such a position as to allow formation of a sugar-phosphate chemical linkage between the 3' end of one moiety and the 5' end of the other moiety.

    摘要翻译： 本发明提供了标记的核苷酸探针和用于通过使用衔接互补物（通过使用保持蛋白质）的桥接补体，通过探针的两个组分的偶联或并入来合成标记的核苷酸探针的方法，即靶识别序列部分和信号产生部分 这两个部分处于这样的位置，以允许在一个部分的3'末端和另一个部分的5'末端之间形成糖 - 磷酸酯化学键。

    Cuvette for receiving liquid sample
    5.
    发明授权
    Cuvette for receiving liquid sample 失效
    用于接收液体样品的比色杯

    公开(公告)号：US4979821A

    公开(公告)日：1990-12-25

    申请号：US388193

    申请日：1989-08-01

    申请人： Ernest G. Schutt ,  David L. Greenwood ,  Karin L. Utberg

    发明人： Ernest G. Schutt ,  David L. Greenwood ,  Karin L. Utberg

    IPC分类号： G01N33/543 ,  A61B6/08 ,  G01J1/04 ,  G01J3/02 ,  G01N21/21 ,  G01N21/47 ,  G01N21/55 ,  G01N33/553 ,  G01N33/58 ,  G02B17/06 ,  G02B27/20

    CPC分类号： G01N21/552 ,  G01N33/553 ,  G01N33/585 ,  G01J3/0216 ,  G01J3/0243 ,  G01J3/0297 ,  G01N21/21 ,  G01N21/47 ,  Y10S436/807

    摘要： A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.

    摘要翻译： 提供了一种新的免疫测定系统，用于以异构方式检测溶液中的配体或配体结合配偶体。 本发明依赖于通过由免疫反应引入到界面的胶体金标记的存在而扰乱的ev逝波的背散射光的检测。 接口中存在的ev逝波依次是全反射入射光波的结果。 在高于临界角的后角处放置检测器确保了优异的信噪比。 提供了用于扫描，检测和操纵包括散射的全内反射免疫测定系统的光的装置和方法。

    Particle washing and separation method
    6.
    发明授权
    Particle washing and separation method 失效
    颗粒洗涤和分离方法

    公开(公告)号：US4698311A

    公开(公告)日：1987-10-06

    申请号：US676973

    申请日：1985-10-30

    申请人： Shelby J. Hall ,  David J. Olekna ,  Donald M. Davies, Jr.

    发明人： Shelby J. Hall ,  David J. Olekna ,  Donald M. Davies, Jr.

    IPC分类号： G01N33/49 ,  G01N1/00 ,  B01D33/02 ,  B04B5/02

    CPC分类号： G01N33/491 ,  Y10T436/101666 ,  Y10T436/106664

    摘要： A device is described for preparing a standardized concentration of washed particles suspended in a fluid. The preferred method is directed to the preparation from whole blood specimens of a standardized concentration of washed red cells in isotonic solution using a device comprising a test tube formed at the bottom end with a nipple-like receptacle having a predetermined volumetric capacity. Whole blood is mixed with isotonic solution in the device which is then centrifuged causing the red cells to be first washed and then packed into the receptacle. The liquid contents of the device are then decanted without disturbing the packed red cells. Thereafter, the red cells are reconstituted to the desired concentration by adding isotonic solution to a predetermined level in the tube and mixing the cells into the isotonic solution.

    摘要翻译： 描述了一种用于制备悬浮在流体中的洗涤颗粒的标准浓度的装置。 优选的方法是使用包含在底端形成的具有预定体积容量的乳头状容器的试管的装置在等渗溶液中使用标准浓度的洗涤红细胞的全血标本制备。 将全血与装置中的等渗溶液混合，然后离心，使红细胞首先洗涤，然后装入容器中。 然后将装置的液体内容物倾析，而不会干扰包装的红细胞。 此后，通过在管中加入等渗溶液至预定水平并将细胞混合到等渗溶液中，将红细胞重构成所需浓度。

    Immunoassay methods employing patterns for the detection of soluble and cell surface antigens
    9.
    发明授权
    Immunoassay methods employing patterns for the detection of soluble and cell surface antigens 失效
    使用模式检测可溶性和细胞表面抗原的免疫测定方法

    公开(公告)号：US4487839A

    公开(公告)日：1984-12-11

    申请号：US455765

    申请日：1983-01-05

    申请人： Louis A. Kamentsky

    发明人： Louis A. Kamentsky

    IPC分类号： C12Q1/04 ,  G01N21/86 ,  G01N33/543 ,  G01N33/569 ,  G01N33/80 ,  G01N33/52 ,  G01N33/58

    CPC分类号： G01N21/8483 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/56966 ,  G01N33/80 ,  Y10S436/80 ,  Y10S436/804 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/823

    摘要： Methods for determining the presence of antigens or antibodies in an aqueous sample or presence of antigens on the surface of cells. A preferred embodiment employs fluorescent antigens which compete with the sample antigens for antibody binding sites. The antibodies are deposited on a support surface means in alternating patterns. The surface means and fluorescence detector are translocated with respect to each other and a signal generated by the detection of the repeating pattern of fluorescence. The signal is analyzed by means of a gated integrator responsive to a gate track control means also located on the surface means. Immunoassay methods having increased sensitivity are thereby obtained.

    摘要翻译： 用于测定水样品中抗原或抗体存在或细胞表面上存在抗原的方法。 优选的实施方案使用与样品抗原竞争抗体结合位点的荧光抗原。 抗体以交替的模式沉积在支撑表面上。 表面装置和荧光检测器相对于彼此移位，并且通过检测重复荧光图案产生的信号。 通过门控积分器来响应于也位于表面装置上的门轨道控制装置来分析信号。 由此获得具有增加的灵敏度的免疫测定方法。