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    Protein isolate and process for the production thereof
    1.
    发明授权
    Protein isolate and process for the production thereof 有权

    公开(公告)号：US11832633B2

    公开(公告)日：2023-12-05

    申请号：US16764739

    申请日：2018-11-14

    申请人： AVRIL ,  UNIVERSITE DE LORRAINE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

    发明人： Claire Defaix ,  Romain Kapel ,  Olivier Galet

    IPC分类号： A23J1/14 ,  C07K1/34 ,  C07K14/76

    CPC分类号： A23J1/142 ,  A23J1/148 ,  C07K1/34 ,  C07K14/76 ,  C12Y301/03

    摘要： A process for producing a protein isolate from an oilseed meal, and the isolate thus obtained, said isolate comprising proteins and an amount of 4 wt. % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate. The process may comprise the following steps:



    a) providing an oilseed meal;
    b) mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a solid phase;
    c) separating said solid phase from said slurry,
    d) mixing said separated solid phase with a second aqueous solvent at a pH ranging from 1 to 3.5, preferably from 2 to 3, to form a mixture said mixture having a liquid phase;
    e) separating said liquid phase from said mixture formed in step d);
    f) f1) mixing the separated liquid phase to a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase;
    and/or
    f2) mixing the separated liquid to a salt, to obtain a resulting liquid composition having a molar concentration of said salt ranging from 0.05M to 0.5M, at a temperature ranging from 40° C. to 70° C., to obtain a mixture having a liquid phase and a solid phase;
    g) precipitating a solid phase from the liquid of step f) for example by a cooling down step of the mixture to a temperature of 30° C. or less;
    h) separating said solid precipitate from the liquid of step g) said liquid comprising a water-rich liquid phase and an oil-rich liquid phase;
    i) separating said water-rich liquid phase from said oil-rich liquid phase,
    j) subjecting said water-rich liquid phase obtained in step i) to one or several membrane filtration(s) to obtain a protein isolate; and
    k) optionally, drying said protein isolate to obtain a dry protein isolate.