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    DNA sequencing process using stable isotopes
    1.
    发明授权
    DNA sequencing process using stable isotopes 失效
    使用稳定同位素进行DNA测序

    公开(公告)号：US5002868A

    公开(公告)日：1991-03-26

    申请号：US221734

    申请日：1988-07-20

    申请人： K. Bruce Jacobson ,  Harold W. Schmitt

    发明人： K. Bruce Jacobson ,  Harold W. Schmitt

    IPC分类号： C07H21/00 ,  C12Q1/68

    CPC分类号： C12Q1/6869 ,  C07H21/00 ,  C12Q1/6816 ,  Y10S435/81 ,  Y10S436/80 ,  Y10S436/803 ,  Y10S436/805 ,  Y10T436/143333

    摘要： A DNA sequencing process using specific stable isotopes associated with specific terminators for labels. The process includes a step of incorporating a stable isotope in at least one of the deoxynucleoside triphosphates and/or the dideoxynucleoside triphosphates such that a terminated strand has included within it or at the end a stable isotope such as an isotope of sulphur. Replicated strands are then separated by performing gel electrophoresis thereon. The location of the DNA strand with the stable isotope assigned to a terminator is analyzed preferably by resonance ionization spectroscopy. The stable isotopes can be chosen such that specific labels are assigned to at least one, and preferably to each base, in the dideoxynucleoside triphosphates. In the preferred embodiment, each of the bases (A, T, G and C) are associated with a specific stable isotopic label, and can be analyzed in a single track which enhances the accuracy of the sequencing process.

    摘要翻译： 使用与特定终止子相关的特定稳定同位素进行标记的DNA测序过程。 该方法包括在至少一种脱氧核苷三磷酸和/或双脱氧核苷三磷酸中加入稳定同位素的步骤，使得封端的链在其内或末端包含稳定同位素如硫的同位素。 然后通过在其上进行凝胶电泳分离复制的链。 优选通过共振电离光谱法分析具有分配给终止子的稳定同位素的DNA链的位置。 可以选择稳定的同位素，使得特定的标记分配给双脱氧核苷三磷酸中的至少一个，优选分配给每个碱基。 在优选实施方案中，每个碱基（A，T，G和C）与特定的稳定同位素标记相关联，并且可以在增强测序过程的准确性的单个轨道中进行分析。

    Micromechanical antibody sensor
    3.
    发明授权
    Micromechanical antibody sensor 失效
    微机械抗体传感器

    公开(公告)号：US06289717B1

    公开(公告)日：2001-09-18

    申请号：US09281032

    申请日：1999-03-30

    申请人： Thomas G. Thundat ,  K. Bruce Jacobson ,  Mitchel J. Doktycz ,  Stephen J. Kennel ,  Robert J. Warmack

    发明人： Thomas G. Thundat ,  K. Bruce Jacobson ,  Mitchel J. Doktycz ,  Stephen J. Kennel ,  Robert J. Warmack

    IPC分类号： G01N1910

    CPC分类号： G01N33/54373

    摘要： A sensor apparatus is provided using a microcantilevered spring element having a coating of a detector molecule such as an antibody or antigen. A sample containing a target molecule or substrate is provided to the coating. The spring element bends in response to the stress induced by the binding which occurs between the detector and target molecules. Deflections of the cantilever are detected by a variety of detection techniques. The microcantilever may be approximately 1 to 200 &mgr;m long, approximately 1 to 50 &mgr;m wide, and approximately 0.3 to 3.0 &mgr;m thick. A sensitivity for detection of deflections is in the range of 0.01 nanometers.

    摘要翻译： 使用具有检测器分子如抗体或抗原的涂层的微悬臂弹簧元件提供传感器装置。 将包含靶分子或基底的样品提供给涂层。 弹簧元件响应于由检测器和靶分子之间发生的结合引起的应力而弯曲。 通过各种检测技术检测悬臂的偏转。 该微悬臂梁的长度可以为约1至200μm，宽度大约为1至50μm，厚度为0.3至3.0μm。 检测偏转的灵敏度在0.01纳米的范围内。

    Method and apparatus for retaining polycrystalline diamond cutters in a drill bit body of matrix or steel
    4.
    发明申请
    Method and apparatus for retaining polycrystalline diamond cutters in a drill bit body of matrix or steel 审中-公开
    用于将多晶金刚石切割器保持在基体或钢的钻头体中的方法和装置

    公开(公告)号：US20090038442A1

    公开(公告)日：2009-02-12

    申请号：US12221836

    申请日：2008-08-07

    申请人： James Shamburger ,  Bruce Jacobson

    发明人： James Shamburger ,  Bruce Jacobson

    IPC分类号： B21K5/04

    CPC分类号： B23P5/00 ,  B23B2226/315 ,  B23B2226/36 ,  B23B2240/21 ,  B23B2250/125

    摘要： As a more friendly, environmentally sounder replacement process for retaining PDC cutters in drill bits, compared to the known high temperature metallic brazing processes, a process is described for using a low temperature resin which cures in the range of 350° F. to 450° F., while having a melting point below 1250° F., to prevent degradation to the PDC cutters usually occurring at 1250° F. in the drill bit repair cycle.

    摘要翻译： 与已知的高温金属钎焊工艺相比，作为一种用于保持钻头中PDC刀具的更加友好，更环保的更换方法，描述了使用在350°F至450°范围内固化的低温树脂的方法 F.同时具有低于1250°F的熔点，以防止在钻头修复周期中通常发生在1250°F的PDC切割机的劣化。

    DNA sequencing, mapping, and diagnostic processes using hybridization chips and unlabeled DNA
    6.
    发明授权
    DNA sequencing, mapping, and diagnostic processes using hybridization chips and unlabeled DNA 失效
    使用杂交芯片和未标记的DNA进行DNA测序，绘图和诊断过程

    公开(公告)号：US5821060A

    公开(公告)日：1998-10-13

    申请号：US691614

    申请日：1996-08-02

    申请人： Heinrich F. Arlinghaus ,  K. Bruce Jacobson

    发明人： Heinrich F. Arlinghaus ,  K. Bruce Jacobson

    IPC分类号： C12Q1/68 ,  C40B60/14 ,  B01D59/44 ,  G01N33/48

    CPC分类号： C12Q1/6872 ,  C12Q1/6874 ,  B01J2219/00378 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  C40B60/14 ,  Y10T436/24

    摘要： A process for deoxyribonucleic acid (DNA) sequencing, mapping, and diagnostics which utilizes the differences between the chemical composition of DNA and that of peptide nucleic acids (PNAs) to provide DNA sequencing, mapping, or diagnostics using natural DNA fragments, rather than using radioisotopes, stable isotopes or fluorescent substances to label the DNAs. The process includes the steps of hybridizing PNA segments to complementary DNA segments which are fixed to a hybridization surface, or hybridizing DNA segments to complementary PNA segments which are fixed to a hybridization surface, and using mass spectrometric or non-mass spectrometric techniques to analyze the extent of hybridization at each potential hybridization site.

    摘要翻译： 使用脱氧核糖核酸（DNA）测序，绘图和诊断的方法，其利用DNA与肽核酸（PNA）的化学成分之间的差异来提供使用天然DNA片段的DNA测序，测绘或诊断，而不是使用 放射性同位素，稳定同位素或荧光物质来标记DNA。 该方法包括以下步骤：将PNA片段与固定于杂交表面的互补DNA片段杂交，或将DNA片段与固定于杂交表面的互补PNA片段杂交，并使用质谱或非质谱技术分析 每个潜在杂交位点的杂交程度。

    DNA sequencing, mapping, and diagnostic processes using hybridization and stable isotope labels of DNA
    7.
    发明授权
    DNA sequencing, mapping, and diagnostic processes using hybridization and stable isotope labels of DNA 失效
    使用DNA的杂交和稳定同位素标记进行DNA测序，测绘和诊断过程

    公开(公告)号：US5780232A

    公开(公告)日：1998-07-14

    申请号：US654181

    申请日：1996-05-28

    申请人： Heinrich F. Arlinghaus ,  K. Bruce Jacobson

    发明人： Heinrich F. Arlinghaus ,  K. Bruce Jacobson

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q1/6869 ,  Y10S435/81

    摘要： A DNA sequencing, mapping, and diagnostic process which includes the steps of labeling nucleotide segments or peptide nucleic acids (PNAs) with one or more atoms of specific stable or long-lived radioactive isotopes of a selected element that do not normally occur in DNAs, ODNs or PNAs such that the nucleotide segment or PNA has specific stable or long-lived radioactive isotope of a specified selected element at a terminal or an interior position; hybridizing the labeled nucleotide segment or PNA to complementary nucleic acid segments or PNAs which are fixed on a hybridization surface; and, using mass spectrometric techniques, including RIS, to analyze the presence and position of the labeled hybridized nucleotide segments or PNAs which are bound to the fixed nucleotide segments or PNAs.

    摘要翻译： DNA测序，测绘和诊断过程，其包括将核苷酸片段或肽核酸（PNA）与一个或多个特定稳定或长寿命的放射性同位素原子进行标记的步骤，所述放射性同位素通常不存在于DNA中， ODN或PNA，使得核苷酸片段或PNA在终端或内部位置具有特定的选定元素的特定稳定或长寿命的放射性同位素; 将标记的核苷酸片段或PNA与固定在杂交表面上的互补核酸片段或PNA杂交; 并且使用包括RIS的质谱技术来分析与固定的核苷酸区段或PNA结合的标记的杂交核苷酸区段或PNA的存在和位置。

    Mold used in manufacture of drill bits and method of forming same
    9.
    发明申请
    Mold used in manufacture of drill bits and method of forming same 审中-公开
    用于制造钻头的模具及其形成方法

    公开(公告)号：US20100101747A1

    公开(公告)日：2010-04-29

    申请号：US12590561

    申请日：2009-11-10

    申请人： Michael Tomczak ,  Vincente Salvo ,  Juan Rios ,  Bruce Jacobson

    发明人： Michael Tomczak ,  Vincente Salvo ,  Juan Rios ,  Bruce Jacobson

    IPC分类号： B22D19/04 ,  B22C9/02 ,  B22C9/22 ,  B22C9/10

    CPC分类号： B22D19/06 ,  B22C9/02 ,  B22C9/10 ,  B22C9/22 ,  B22D19/04 ,  B22D23/00 ,  E21B10/42

    摘要： A mold usable to form a drill bit body and a method for forming such a mold are disclosed. A shell is provided, the shell having a cavity. Fusible material, such as foundry sand, is provided into the cavity, then fused to form a solid insert within the shell. The solid insert is then modified, such as by machining, to form a mold having features usable to form a drill bit body having desired characteristics. Materials for forming the drill bit body can then be provided into the mold, for heating and cooling. Then, the drill bit body and mold can be removed from the shell. The shell is thereby reusable, while the mold, being formed from inexpensive fusible materials, can be removed or destroyed, if necessary, to free the drill bit body.

    摘要翻译： 公开了一种可用于形成钻头体的模具和用于形成这种模具的方法。 提供壳体，壳体具有空腔。 将可熔材料（例如铸造砂）提供到空腔中，然后熔化以在壳体内形成固体插入物。 然后，通过机加工将固体镶嵌体改进，以形成具有可用于形成具有期望特性的钻头体的特征的模具。 然后可以将用于形成钻头体的材料提供到模具中用于加热和冷却。 然后，钻头体和模具可以从壳体上移除。 因此，壳可以重复使用，而由便宜的可熔材料形成的模具可以被去除或破坏，如果需要的话，以释放钻头体。