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    PROTEIN CAPABLE OF BINDING SPECIFICALLY TO IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND
    2.
    发明申请
    PROTEIN CAPABLE OF BINDING SPECIFICALLY TO IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND 审中-公开
    蛋白质能够特异性结合免疫球蛋白和免疫调节蛋白结合亲和力配体

    公开(公告)号：US20130096276A1

    公开(公告)日：2013-04-18

    申请号：US13636584

    申请日：2011-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C07K14/195

    CPC分类号： C07K14/195 ,  C07K1/22 ,  C07K14/31 ,  C07K16/065 ,  C07K16/32 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/92

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.

    摘要翻译： 本发明的目的是与已知的工程改造的蛋白A配体相比，在酸性条件下产生具有更好抗体解离性能的新型工程蛋白A配体。 本发明提供对免疫球蛋白具有亲和性的蛋白质，包括通过将衍生自蛋白A​​的E，D，A，B和C结构域的任何氨基酸序列导入的氨基酸序列，至少一个氨基酸 在对应于A，B和C结构域（E结构域的29至35位，D结构域的34至40位）的31至37位的氨基酸残基的任何一个或多个取代，其在所有 结构域，对于免疫球蛋白Fab区域的亲和力低于在引入取代之前具有氨基酸序列的蛋白质的蛋白质。

    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand
    3.
    发明授权
    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand 有权
    对免疫球蛋白具有亲和力的蛋白质和免疫球蛋白结合亲和配体

    公开(公告)号：US09403883B2

    公开(公告)日：2016-08-02

    申请号：US13258655

    申请日：2010-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C07K14/31 ,  C07K14/47 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38

    CPC分类号： C07K14/47 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3244 ,  B01J20/3274 ,  C07K14/31

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.

    摘要翻译： 本发明的一个目的是在酸存在下产生具有比常规工程改造的蛋白A配体更好的抗体解离性能的新型工程改造的蛋白A配体，本发明的另一个目的是产生一种新的工程改造的蛋白A配体，其具有 耐碱性较高。 本发明提供一种对免疫球蛋白具有亲和性的蛋白质，其包括衍生自蛋白A​​的E，D，A，B和C结构域中的任一个的氨基酸序列，其中氨基酸序列中至少一个Gly残基为 被除Ala以外的氨基酸所取代，并且该蛋白质对包含氨基酸序列的蛋白质的免疫球蛋白Fab区域的亲和力低于其中Gly残基被Ala置换的蛋白质，本发明还提供 对于免疫球蛋白具有亲和力的蛋白质，与相应的结构域相比，其在碱性条件下具有改善的化学稳定性。

    PROTEIN HAVING AFFINITY FOR IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND
    4.
    发明申请
    PROTEIN HAVING AFFINITY FOR IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND 有权
    具有免疫球蛋白的免疫原性蛋白质和免疫球蛋白结合亲和力配体

    公开(公告)号：US20120208234A1

    公开(公告)日：2012-08-16

    申请号：US13258655

    申请日：2010-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C12P21/02 ,  C07K19/00 ,  C12N15/31 ,  C12N15/62 ,  C12N1/21 ,  C07K16/12 ,  C07K1/14 ,  C07K14/31 ,  C12N15/74

    CPC分类号： C07K14/47 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3244 ,  B01J20/3274 ,  C07K14/31

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.

    摘要翻译： 本发明的一个目的是在酸存在下产生具有比常规工程改造的蛋白A配体更好的抗体解离性能的新型工程改造的蛋白A配体，本发明的另一个目的是产生一种新的工程改造的蛋白A配体，其具有 耐碱性较高。 本发明提供一种对免疫球蛋白具有亲和性的蛋白质，其包括衍生自蛋白A​​的E，D，A，B和C结构域中的任一个的氨基酸序列，其中氨基酸序列中至少一个Gly残基为 被除Ala以外的氨基酸所取代，并且该蛋白质对包含氨基酸序列的蛋白质的免疫球蛋白Fab区域的亲和力低于其中Gly残基被Ala置换的蛋白质，本发明还提供 对于免疫球蛋白具有亲和力的蛋白质，与相应的结构域相比，其在碱性条件下具有改善的化学稳定性。

    Decarbamylase isolated from comamonas or blastobacter
    7.
    发明授权
    Decarbamylase isolated from comamonas or blastobacter 失效
    从comamonas或blastobacter分离的脱氨基甲酰酶

    公开(公告)号：US5863785A

    公开(公告)日：1999-01-26

    申请号：US479639

    申请日：1995-06-07

    申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Yasuhiro Ikenaka ,  Kazuyoshi Yajima ,  Yukio Yamada ,  Hirokazu Nanba ,  Masayuki Takano ,  Satomi Takahashi

    发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Yasuhiro Ikenaka ,  Kazuyoshi Yajima ,  Yukio Yamada ,  Hirokazu Nanba ,  Masayuki Takano ,  Satomi Takahashi

    IPC分类号： C12P13/04 ,  C12P41/00 ,  C12N9/78 ,  C12N9/80

    CPC分类号： C12P13/04 ,  C12P41/009 ,  Y10S435/874 ,  Y10S435/911

    摘要： In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.

    摘要翻译： 在制备D-α-氨基酸的方法中，其中相应于通式为：苯基，取代或未取代的烷基或噻吩基的N-氨基甲酰基-D-α-氨基酸被转化 通过微生物酶在水性介质中与对应于以下通式的D-α-氨基酸反应：其中R与上述定义相同，由Comamonas属，Blastobacter，Alcaligenes，Sporosarcina属的微生物产生的脱氨基甲酰酶， 使用根瘤菌，Bradyrhizobium或节杆菌作为将N-氨基甲酰基-D-α-氨基酸转化为D-α-氨基酸的酶。 N-氨基甲酰基-D-α-氨基酸向D-α-氨基酸的转化在中性至碱性pH范围内进行。

    Blower
    8.
    发明授权
    Blower 有权
    鼓风机

    公开(公告)号：US08745815B2

    公开(公告)日：2014-06-10

    申请号：US13372665

    申请日：2012-02-14

    申请人： Masayuki Takano ,  Takeshi Maeno

    发明人： Masayuki Takano ,  Takeshi Maeno

    IPC分类号： A47L5/24 ,  F04D25/08

    CPC分类号： F04D25/084 ,  A01G20/43

    摘要： A handheld blower has a body including a combustion engine and a blower section driven by the combustion engine, and a handle coupled to the body. The blower section has an air outlet directed forward with respect to an operator. The handle has a grip portion extending in a front-back direction with respect to the operator, a front arm extending from a front portion of the grip portion to the body, and a rear arm extending from a rear portion of the grip portion to the body. The front arm and the rear arm are more flexible in the front-back direction than in directions perpendicular to the front-back direction.

    摘要翻译： 手持式鼓风机具有主体，其包括内燃机和由内燃机驱动的鼓风机部分，以及与主体相连的手柄。 鼓风机部分具有相对于操作者向前指示的出气口。 手柄具有相对于操作者在前后方向上延伸的抓握部分，从把手部分的前部延伸到主体的前臂以及从把手部分的后部延伸到主体的后臂 身体。 前臂和后臂在前后方向上比在垂直于前后方向的方向上更柔性。

    WORK MACHINE
    9.
    发明申请
    WORK MACHINE 失效
    工作机器

    公开(公告)号：US20090048743A1

    公开(公告)日：2009-02-19

    申请号：US12096829

    申请日：2006-08-29

    申请人： Eiji Akahane ,  Christopher Michael Burton ,  Nobumi Toyoura ,  Shigeru Kojima ,  Masayuki Takano

    发明人： Eiji Akahane ,  Christopher Michael Burton ,  Nobumi Toyoura ,  Shigeru Kojima ,  Masayuki Takano

    IPC分类号： G08B21/02 ,  G05G1/01 ,  G05G1/30 ,  B60Q1/00

    CPC分类号： E02F9/2004 ,  E02F9/2025 ,  Y10T74/20012 ,  Y10T74/20396 ,  Y10T74/20528 ,  Y10T74/2054

    摘要： To provide a work machine in which traveling devices and an implement device can be operated by common operating levers, wherein switching operation is easy and a changeover is easily confirmed. Inside a cab of the work machine, there are provided travel pedals, with which the traveling devices can be operated, and operating levers, with which the traveling devices and the implement device can be operated. A changeover switch is provided on a console positioned in the vicinity of one side of an operating sheet. The changeover switch switches over between a traveling mode, in which traveling operation is possible by the operating levers, and a work mode, in which implement operation is possible by the operating levers. The work machine has a controller which makes it possible to work by the operating levers when the travel pedal is not stepped on in the case where the work mode or traveling mode is selected by the changeover switch, and makes it possible to travel by the operating levers only when the travel pedal is stepped on in the case where the traveling mode is selected by the changeover switch.

    摘要翻译： 为了提供一种工作机械，其中行驶装置和工具装置可由普通操作杆操作，其中切换操作容易并且容易确认切换。 在作业机的驾驶室内设有可以操作行走装置的行驶踏板，以及可以操作行驶装置和工具装置的操作杆。 在位于操作片一侧附近的控制台上设置有切换开关。 切换开关在通过操作杆进行行驶操作的行驶模式和通过操作杆进行实施操作的作业模式之间切换。 作业机具有控制器，当通过切换开关选择工作模式或行驶模式的情况下，当行走踏板不踩踏时，能够通过操作杆进行操作，并且可以通过操作 只有在通过切换开关选择了行驶模式的情况下踏步踏板时，才能够进行操作。