利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

8 条记录 (耗时 0.015 秒)

    Vaccine delivery system
    3.
    发明授权
    Vaccine delivery system 失效
    疫苗输送系统

    公开(公告)号：US06180111B2

    公开(公告)日：2001-01-30

    申请号：US09081576

    申请日：1998-05-19

    申请人： Daniel C. Stein ,  Charles K. Stover

    发明人： Daniel C. Stein ,  Charles K. Stover

    IPC分类号： A61K39095

    CPC分类号： C07K14/22 ,  A61K39/00 ,  A61K39/095 ,  A61K39/39 ,  A61K2039/55555 ,  A61K2039/55594 ,  Y02A50/401 ,  Y10S530/812

    摘要： The invention relates to a hyperblebbing strain of Neisseria gonorrhoeae which produces large amounts of blebs useful for production of blebosomes containing antigens for use as a vaccine delivery vehicle or as a diagnostic reagent. The invention also relates to a method for producing high levels of a desired protein in purified form using the hyperblebbing strain of N. gonorrhoeae, and to a vaccine delivery systems containing the blebosomes expressing the desired antigen.

    摘要翻译： 本发明涉及淋病奈瑟氏球菌的超高度吸附菌株，其产生大量可用于产生含有用作疫苗递送载体或作为诊断试剂的抗原的含有抗体的泡沫的泡沫。 本发明还涉及使用淋病奈瑟氏球菌的超耐热菌株以及包含表达所需抗原的小泡的疫苗递送系统来产生高水平的纯化形式的所需蛋白质的方法。

    QUICK DEPLOYMENT OF STORED EQUIPMENT
    6.
    发明申请
    QUICK DEPLOYMENT OF STORED EQUIPMENT 审中-公开
    快速部署存储设备

    公开(公告)号：US20120018478A1

    公开(公告)日：2012-01-26

    申请号：US13145940

    申请日：2010-01-25

    申请人： Robert J. Hanna ,  Jonathan M. Gumbert ,  Daniel C. Stein ,  Miguel T. Sotello

    发明人： Robert J. Hanna ,  Jonathan M. Gumbert ,  Daniel C. Stein ,  Miguel T. Sotello

    IPC分类号： A45F3/04 ,  B23P19/04

    CPC分类号： A45F3/04 ,  A45C7/0059 ,  Y10T29/49826

    摘要： Provided is a storage compartment allowing quick deployment of emergency equipment stored therein, where the compartment comprises a first door that is opened and the equipment deployed in response to pulling a pull cord connected to the compartment or an electronic signal. Also provided is a bag comprising emergency equipment within a compartment deployable by a user while wearing the bag, where the bag is configured to deploy the piece of equipment in response to pulling a pull cord connected to the bag or an electronic signal. Additionally, a bag configured to house a gas mask and gas mask equipment is provided, where the bag is configured to deploy the gas mask from the bag in response to an action by a user.

    摘要翻译： 提供了一种容纳快速部署存储在其中的应急设备的储藏室，其中隔室包括打开的第一门，并且响应于拉动连接到隔间的拉绳而部署设备或电子信号。 还提供了一种袋子，其包括在穿着袋子的情况下由用户可展开的隔间内的应急设备，其中袋被配置成响应于拉动连接到袋子的拉绳或电子信号来配置该设备。 另外，提供了一种构造成容纳防毒面具和防毒面具设备的袋子，其中袋被配置成响应于使用者的动作从袋子部署防毒面具。

    Mutant strain of E. coli for detection of methyltransferase clones
    8.
    发明授权
    Mutant strain of E. coli for detection of methyltransferase clones 失效
    用于检测甲基转移酶克隆的大肠杆菌的突变菌株

    公开(公告)号：US5491060A

    公开(公告)日：1996-02-13

    申请号：US956578

    申请日：1992-10-06

    申请人： Daniel C. Stein ,  Andrejez S. Piekarowicz ,  Robert T. Yuan

    发明人： Daniel C. Stein ,  Andrejez S. Piekarowicz ,  Robert T. Yuan

    IPC分类号： C12N9/10 ,  C12N9/22 ,  C12N15/54 ,  C12N15/70 ,  C12Q1/68 ,  C12N15/09 ,  C12N15/55

    CPC分类号： C12N9/22 ,  C12N15/70 ,  C12N9/1007

    摘要： E. coli bacterial strains encoding a restriction gene that degrades methylated DNA, and prevents cloning of genes expressing the methyltransferase responsible for methylation, are mutated by a chemical or physical mutagen, so as to make the restriction enzyme temperature sensitive. Mutant cells are rendered competent, and plasmids expected or known to contain genes encoding methyltransferase enzymes are introduced. The transformants grow at the permissive temperature, where the restriction enzyme system is inactivated due to the mutated gene. Successful clones, expressing a methyltransferase, can be quickly identified by those which grow at the permissive temperature, but not at the non-permissive temperature. The valuable methyltransferases, as well as restriction enzymes associated therewith, can accordingly be recovered in large quantity.

    摘要翻译： 通过化学或物理诱变剂突变编码降解甲基化DNA的限制性基因并防止克隆表达甲基化的甲基转移酶的基因的大肠杆菌细菌菌株，使得限制酶温度敏感。 引入突变体细胞，并引入预期或已知含有编码甲基转移酶基因的质粒。 转化体在允许的温度下生长，其中限制酶系统由于突变的基因而失活。 表达甲基转移酶的成功克隆可以通过在许可温度下生长而不是在非允许温度下生长的那些快速鉴定。 因此，可以大量回收有价值的甲基转移酶以及与其相关的限制酶。