公开(公告)号：US20090023589A1

公开(公告)日：2009-01-22

申请号：US12109189

申请日：2008-04-24

申请人： Douglas Levinson ,  Christine Kitsos ,  Irena MeInikova ,  Christopher McNulty

发明人： Douglas Levinson ,  Christine Kitsos ,  Irena MeInikova ,  Christopher McNulty

IPC分类号： C40B20/04

CPC分类号： G01N33/5023 ,  G01N33/502 ,  G01N33/56966

摘要： The present invention is directed to methods for identifying agents which affect cell state. The instant invention provides rapid and efficient methods for identifying agents which affect cell state. Methods are directed toward the screening of complex combinations of agents for their ability to affect cell state. In one embodiment, cells are incubated under suitable conditions and subjected to different agents. After an appropriate amount of time, the cells are assayed to determine what, if any, characteristics they possess. Cell characteristics can be organized in a manner such that different and novel cell states can be identified.

摘要翻译： 本发明涉及用于鉴定影响细胞状态的药剂的方法。 本发明提供用于鉴定影响细胞状态的试剂的快速和有效的方法。 方法的目的是筛选复合物的组合，使其能够影响细胞状态。 在一个实施方案中，将细胞在合适的条件下培养并进行不同的试剂。 在适当的时间后，测定细胞以确定它们具有的特征（如果有的话）。 可以以能够识别不同和新颖的细胞状态的方式来组织细胞特征。

公开(公告)号：US20070021929A1

公开(公告)日：2007-01-25

申请号：US11467096

申请日：2006-08-24

申请人： Anthony Lemmo ,  Javier Gonzalez-Zugasti ,  Michael Cima ,  Douglas Levinson ,  Alasdair Johnson ,  Orn Almarsson ,  Hongming Chen ,  Christopher McNulty

发明人： Anthony Lemmo ,  Javier Gonzalez-Zugasti ,  Michael Cima ,  Douglas Levinson ,  Alasdair Johnson ,  Orn Almarsson ,  Hongming Chen ,  Christopher McNulty

IPC分类号： G06F19/00

CPC分类号： G01N35/0092 ,  G01N35/00613 ,  G01N35/00712 ,  G01N2015/1493

摘要： Computer-controlled automated high-throughput systems can be used to design, prepare, process, screen, and analyze a large number of samples in removable sample vials each containing a compound of interest formulated with differing component combinations and varying concentrations. The computer-controlled methods of the present invention allow for a determination of the effects of additional or inactive components, such as excipients, carriers, enhancers, adhesives, additives, and the like, on the compound of interest, such as a pharmaceutical. The invention thus encompasses the computer systems, computer methods, and computer-program products for computer-controlled automated high-throughput testing of experimental formulations in order to identify experimental formulations that can be further processed. Identified experimental formulations from multiple arrays can be removed and re-arrayed together to form a new array for further processing.

摘要翻译： 计算机控制的自动化高通量系统可用于设计，制备，处理，筛选和分析大量可拆卸样品瓶中的样品，每个样品瓶均含有不同成分组合和不同浓度配制的感兴趣的化合物。 本发明的计算机控制的方法允许确定附加或非活性组分如赋形剂，载体，增强剂，粘合剂，添加剂等对目的化合物如药物的作用。 本发明因此包括计算机系统，计算机方法和计算机程序产品，用于实验制剂的计算机控制的自动高通量测试，以便鉴定可进一步处理的实验制剂。 来自多个阵列的识别的实验制剂可以被去除并重新排列在一起以形成用于进一步处理的新阵列。

公开(公告)号：US20060141533A1

公开(公告)日：2006-06-29

申请号：US11350213

申请日：2006-02-08

申请人： Douglas Levinson

发明人： Douglas Levinson

IPC分类号： C40B40/02 ,  C40B40/10

CPC分类号： C12Q1/18 ,  B01J2219/00315 ,  B01J2219/00351 ,  B01J2219/00484 ,  B01J2219/00486 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00698 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00756 ,  G01N33/543

摘要： The invention concerns arrays comprising hundreds, thousands, to hundreds of thousands of samples and methods for screening thereof. These methods are useful to optimize, select, and discover compounds, compositions, or conditions that prevent, inhibit, induce, modify, or reverse physical-state transitions, particularly in-vivo physical-state transitions relating to disease causing processes. Such compounds, compositions, or conditions can be exploited to treat (e.g., reverse) or prevent the disease itself, the cause of the disease, or the symptoms of the disease.

公开(公告)号：US20060035211A1

公开(公告)日：2006-02-16

申请号：US11149863

申请日：2005-06-10

申请人： Douglas Levinson ,  Christine Kitsos ,  Irena Melnikova ,  Christopher McNulty

发明人： Douglas Levinson ,  Christine Kitsos ,  Irena Melnikova ,  Christopher McNulty

IPC分类号： C12Q1/00 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

CPC分类号： G01N33/5023 ,  G01N33/502 ,  G01N33/56966

摘要： The present invention is directed to methods for identifying agents which affect cell state. The instant invention provides rapid and efficient methods for identifying agents which affect cell state. Methods are directed toward the screening of complex combinations of agents for their ability to affect cell state. In one embodiment, cells are incubated under suitable conditions and subjected to different agents. After an appropriate amount of time, the cells are assayed to determine what, if any, characteristics they possess. Cell characteristics can be organized in a manner such that different and novel cell states can be identified.

摘要翻译： 本发明涉及用于鉴定影响细胞状态的药剂的方法。 本发明提供用于鉴定影响细胞状态的试剂的快速和有效的方法。 方法的目的是筛选复合物的组合，使其能够影响细胞状态。 在一个实施方案中，将细胞在合适的条件下培养并进行不同的试剂。 在适当的时间后，测定细胞以确定它们具有的特征（如果有的话）。 可以以能够识别不同和新颖的细胞状态的方式来组织细胞特征。

公开(公告)号：US20050119209A1

公开(公告)日：2005-06-02

申请号：US10838852

申请日：2004-05-03

申请人： Douglas Levinson ,  Clare Lloyd ,  Sean McCarthy

发明人： Douglas Levinson ,  Clare Lloyd ,  Sean McCarthy

IPC分类号： A01K67/027 ,  A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C07K19/00 ,  C12N9/64 ,  C12N15/85 ,  C12Q1/68 ,  A61K48/00 ,  C07H21/04

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0325 ,  A01K2267/0368 ,  A01K2267/0375 ,  A01K2267/0381 ,  A01K2267/0387 ,  A01K2267/0393 ,  A61K38/00 ,  C07K14/47 ,  C07K14/70503 ,  C07K19/00 ,  C07K2319/00 ,  C12N9/6467 ,  C12N9/6472 ,  C12Q1/6809

摘要： The present invention relates to methods and compositions for the treatment and diagnosis of immune disorders, especially T helper lymphocyte-related disorders, and also for the treatment of mast cell-related processes and disorders, ischemic disorders and injuries, including ischemic renal disorders and injuries. For example, genes which are differentially expressed within and among T helper (TH) cells and TH cell subpopulations, which include, but are not limited to TH0, TH1 and TH2 cell subpopulations are identified. Genes are also identified via the ability of their gene products to interact with gene products involved in the differentiation, maintenance and effector function of such TH cells and TH cell subpopulations. The genes identified can be used diagnostically or as targets for therapeutic intervention. In this regard, the present invention provides methods for the identification and therapeutic use of compounds as treatments of immune disorders, especially TH cell subpopulation-related disorders. Additionally, methods are provided for the diagnostic evaluation and prognosis of TH cell subpopulation-related disorders, for the identification of subjects exhibiting a predisposition to such conditions, for monitoring patients undergoing clinical evaluation for the treatment of such disorders, and for monitoring the efficacy of compounds used in clinical trials. Methods are also provided for the treatment of symptoms associated with mast cell-related processes or disorders and ischemic disorders and injuries using the genes, gene products and antibodies of the invention.

摘要翻译： 本发明涉及用于治疗和诊断免疫疾病，特别是T辅助淋巴细胞相关病症的方法和组合物，还涉及治疗肥大细胞相关过程和病症，缺血性疾病和损伤，包括缺血性肾病和损伤 。 例如，识别在T辅助细胞（TH）细胞和TH细胞亚群内部和之间差异表达的基因，其包括但不限于TH0，TH1和TH2细胞亚群。 基因也通过其基因产物与涉及TH细胞和TH细胞亚群的分化，维持和效应子功能的基因产物相互作用的能力进行鉴定。 所鉴定的基因可以诊断使用或作为治疗干预的靶标。 在这方面，本发明提供了用于鉴定和治疗性使用化合物作为免疫疾病，特别是TH细胞亚群相关病症治疗的方法。 另外，提供了用于TH细胞亚群相关病症的诊断评估和预后的方法，用于鉴定表现出对这些病症倾向的受试者，用于监测接受临床评估治疗这种病症的患者，以及用于监测 化合物用于临床试验。 还提供了使用本发明的基因，基因产物和抗体治疗与肥大细胞相关过程或病症相关的症状以及缺血性疾病和损伤的方法。

公开(公告)号：US20050130220A1

公开(公告)日：2005-06-16

申请号：US11051517

申请日：2005-01-31

申请人： Anthony Lemmo ,  Javier Gonzalez-Zugasti ,  Michael Cima ,  Douglas Levinson ,  Alasdair Johnson ,  Orn Almarsson ,  Christopher McNulty

发明人： Anthony Lemmo ,  Javier Gonzalez-Zugasti ,  Michael Cima ,  Douglas Levinson ,  Alasdair Johnson ,  Orn Almarsson ,  Christopher McNulty

IPC分类号： B01J19/00 ,  B01L3/14 ,  B01L7/00 ,  C12M1/34 ,  C12Q1/68 ,  C30B7/00 ,  C40B60/14 ,  C40B70/00 ,  G01J3/28 ,  G01J3/44 ,  G01N21/25 ,  G01N21/35 ,  G01N21/65 ,  G01N33/48 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G06F19/00

CPC分类号： G01N35/028 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00283 ,  B01J2219/0031 ,  B01J2219/00335 ,  B01J2219/00344 ,  B01J2219/00495 ,  B01J2219/00547 ,  B01J2219/00587 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00756 ,  B01L3/5082 ,  B01L7/52 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/1805 ,  C30B7/00 ,  C30B29/58 ,  C40B60/14 ,  C40B70/00 ,  G01J3/28 ,  G01N21/253 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/65 ,  G01N35/0099 ,  G01N2021/651 ,  G01N2035/041 ,  G01N2035/042 ,  G01N2035/0425

摘要： Systems and methods are described that allow the high-throughput preparation, processing, and study of arrays of samples, each of which comprises at least one compound. Particular embodiments of the invention allow a large number of experiments to be performed in parallel on samples that comprised of one or more compounds on the milligram or microgram quantities of compounds. Other embodiments of the invention encompass methods and devices for the rapid screening of the results of such experiments, as well as methods and devices for rapidly determining whether or not similarities exist among groups of samples in an array. Particular embodiments of the invention encompass methods and devices for the high-throughput preparation of different forms of compounds (e.g., different crystalline forms), for the discovery of new forms of old compounds, and for the discovery of new methods of producing such forms. Embodiments of the invention also allow for the high-throughput determination of how specific compounds or forms of compounds behave when exposed to other chemicals or environmental conditions.

摘要翻译： 描述了允许高通量制备，处理和研究样品阵列的系统和方法，每个样品包含至少一种化合物。 本发明的具体实施方案允许在由毫克或微克数量的化合物上的一种或多种化合物组成的样品上并行进行大量实验。 本发明的其它实施例包括用于快速筛选这些实验的结果的方法和装置，以及用于快速确定阵列中的样品组之间是否存在相似性的方法和装置。 本发明的具体实施方案包括用于高通量制备不同形式的化合物（例如不同结晶形式）的方法和装置，用于发现新形式的旧化合物，以及发现生产此类形式的新方法。 本发明的实施方案还允许高通量测定当化合物或形式的化合物在暴露于其它化学物质或环境条件时的表现。

公开(公告)号：US20050118636A9

公开(公告)日：2005-06-02

申请号：US09994585

申请日：2001-11-27

申请人： Douglas Levinson

发明人： Douglas Levinson

IPC分类号： G01N33/543 ,  G01N33/53 ,  C12M1/34

CPC分类号： C12Q1/18 ,  B01J2219/00315 ,  B01J2219/00351 ,  B01J2219/00484 ,  B01J2219/00486 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00698 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00756 ,  G01N33/543

摘要： The invention concerns arrays comprising hundreds, thousands, to hundreds of thousands of samples and methods for screening thereof. These methods are useful to optimize, select, and discover compounds, compositions, or conditions that prevent, inhibit, induce, modify, or reverse physical-state transitions, particularly in-vivo physical-state transitions relating to disease causing processes. Such compounds, compositions, or conditions can be exploited to treat (e.g., reverse) or prevent the disease itself, the cause of the disease, or the symptoms of the disease.

摘要翻译： 本发明涉及包括数百个，数千个到数十万个样品的阵列和用于筛选的样品。 这些方法可用于优化，选择和发现预防，抑制，诱导，改变或逆转物理状态转换，特别是与疾病引发过程相关的体内物理状态转换的化合物，组合物或病症。 可以利用这些化合物，组合物或病症来治疗（例如逆转）或预防疾病本身，疾病的病因或疾病的症状。

公开(公告)号：US20070020662A1

公开(公告)日：2007-01-25

申请号：US11467061

申请日：2006-08-24

申请人： Michael Cima ,  Douglas Levinson ,  Donovan Chin ,  Christopher McNulty ,  Christopher Moore ,  Anthony Lemmo ,  Javier Gonzalez-Zugasti ,  Alasdair Johnson ,  Hongming Chen ,  Orn Almarsson

发明人： Michael Cima ,  Douglas Levinson ,  Donovan Chin ,  Christopher McNulty ,  Christopher Moore ,  Anthony Lemmo ,  Javier Gonzalez-Zugasti ,  Alasdair Johnson ,  Hongming Chen ,  Orn Almarsson

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G06F19/00

CPC分类号： G16C20/60 ,  B01J2219/007 ,  B01J2219/00756 ,  G16B15/00 ,  G16B35/00 ,  G16B40/00

摘要： The present invention relates to computer-controlled automated high-throughput systems and/or computer-program products to design, prepare, process, and analyze a large number of samples having experimental formulations each containing a compound of interest formulated with differing component combinations and varying concentrations and component identities. The computer-controlled methods of the present invention allow determination of the effects of additional or inactive components, such as excipients, carriers, enhancers, adhesives, additives, and the like, on the compound of interest, such as pharmaceuticals. The invention thus encompasses the computer systems, computer methods, and computer-program products for computer-controlled automated high-throughput testing of pharmaceutical compositions or formulations in order to determine the overall optimal composition or formulation for an intended use or purpose.

摘要翻译： 本发明涉及计算机控制的自动化高通量系统和/或计算机程序产品，用于设计，制备，处理和分析大量具有实验制剂的样品，每个试样含有不同成分组合配制的感兴趣的化合物， 浓度和组分特征。 本发明的计算机控制方法允许确定附加或不活泼组分如赋形剂，载体，增强剂，粘合剂，添加剂等对目标化合物如药物的作用。 因此，本发明包括计算机系统，计算机方法和用于药物组合物或制剂的计算机控制的自动化高通量测试的计算机程序产品，以便为预期用途或目的确定总体最佳组成或配方。

公开(公告)号：US07108970B2

公开(公告)日：2006-09-19

申请号：US09994585

申请日：2001-11-27

申请人： Douglas Levinson

发明人： Douglas Levinson

IPC分类号： C12Q1/68

CPC分类号： C12Q1/18 ,  B01J2219/00315 ,  B01J2219/00351 ,  B01J2219/00484 ,  B01J2219/00486 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00698 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01J2219/00756 ,  G01N33/543

摘要： The invention concerns arrays comprising hundreds, thousands, to hundreds of thousands of samples and methods for screening thereof. These methods are useful to optimize, select, and discover compounds, compositions, or conditions that prevent, inhibit, induce, modify, or reverse physical-state transitions, particularly in-vivo physical-state transitions relating to disease causing processes. Such compounds, compositions, or conditions can be exploited to treat (e.g., reverse) or prevent the disease itself, the cause of the disease, or the symptoms of the disease.

公开(公告)号：US20050233461A1

公开(公告)日：2005-10-20

申请号：US10517044

申请日：2003-06-04

申请人： Douglas Levinson ,  Kurt Amsler ,  Matthew Peterson

发明人： Douglas Levinson ,  Kurt Amsler ,  Matthew Peterson

IPC分类号： G01N25/14 ,  G01N33/15 ,  G01N33/20

CPC分类号： G01N33/15 ,  G01N25/147

摘要： The invention concerns arrays, systems and methods for screening compounds, compositions or conditions useful to treat or prevent kidney diseases. These methods are useful to discover, optimize and select compounds, compositions, or conditions that prevent, inhibit, induce, modify, or reverse physical-state transitions, particularly in-vivo physical-state transitions relating to disease causing processes such as development and growth of renal calculi. Such compounds, compositions, or conditions can be exploited to treat, prevent or manage the disease itself, the cause of the disease, or the symptoms of the disease.

摘要翻译： 本发明涉及筛选用于治疗或预防肾脏疾病的化合物，组合物或病症的阵列，系统和方法。 这些方法可用于发现，优化和选择预防，抑制，诱导，修饰或逆转物理状态转换的化合物，组合物或病症，特别是与疾病引起过程如发育和生长有关的体内物理状态转化 的肾结石。 可以利用这些化合物，组合物或病症来治疗，预防或治疗疾病本身，疾病的病因或疾病的症状。