METHODS AND DEVICES FOR THE SELECTIVE DETECTION OF MICROORGANISMS
    4.
    发明申请
    METHODS AND DEVICES FOR THE SELECTIVE DETECTION OF MICROORGANISMS 审中-公开
    用于选择性检测微生物的方法和装置

    公开(公告)号:US20120003661A1

    公开(公告)日:2012-01-05

    申请号:US13170045

    申请日:2011-06-27

    CPC分类号: C12Q1/04 C12Q1/24

    摘要: Methods and devices are provided for the rapid and specific detection of target microorganisms, cells, and the like. In one embodiment, the methods involve contacting a target microorganism (e.g., in a sample) with a permeabilization reagent that selectively permeabilizes or lyses the microorganism; contacting the selectively permeabilized microorganism with a detection reagent that is taken into the selectively permeabilized organism or that contacts metabolites or enzymes released by the selectively permeabilized microorganism, where the detection reagent produces a signal in the presence of said metabolites or enzymes; and detecting a signal produced by the detection reagent in the presence of the metabolites or enzymes wherein the strength of the signal indicates the presence and/or amount of the target microorganism in the sample.

    摘要翻译: 提供了用于快速和具体检测靶微生物,细胞等的方法和装置。 在一个实施方案中,所述方法包括使靶微生物(例如,样品)与选择性渗透或裂解微生物的透化试剂接触; 使选择性透过的微生物与被选入透化生物体中的检测试剂接触或接触由选择性透化的微生物释放的代谢物或酶,其中所述检测试剂在所述代谢物或酶存在下产生信号; 以及在所述代谢物或酶的存在下检测由所述检测试剂产生的信号,其中所述信号的强度表示样品中目标微生物的存在和/或量。