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公开(公告)号：US08772017B2
公开(公告)日：2014-07-08
申请号：US13492612
申请日：2012-06-08
申请人： C. Frederick Battrell , John Gerdes , John R. Williford , Denise Maxine Hoekstra , Wayne L. Breidford , Stephen Mordue , John Clemmens , Melud Nabavi , Mark Kokoris
发明人： C. Frederick Battrell , John Gerdes , John R. Williford , Denise Maxine Hoekstra , Wayne L. Breidford , Stephen Mordue , John Clemmens , Melud Nabavi , Mark Kokoris
IPC分类号： C12M1/34
CPC分类号： C12Q1/6834 , B01F11/0071 , B01F13/0059 , B01L3/5027 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/082 , B01L2200/10 , B01L2200/16 , B01L2300/0663 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , C12Q2563/131 , C12Q2525/197 , C12Q2563/143 , C12Q2565/629
摘要： Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.
摘要翻译： 公开了用于从临床样品进行核酸提取，扩增和检测的集成微流控柱。 这些设备是单进入，卫生和一次性的。 该装置能够进行单核或多重核酸靶标检测，例如：用于多种感染剂的测定板或癌细胞类型的测定板。 还公开了在全自动气动控制装置中使用微流控盒的方法。
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公开(公告)号：US20120329142A1
公开(公告)日：2012-12-27
申请号：US13492612
申请日：2012-06-08
申请人： C. Frederick BATTRELL , John GERDES , John R. WILLIFORD , Denise Maxine HOEKSTRA , Wayne L. BREIDFORD , Stephen MORDUE , John CLEMMENS , Melud NABAVI , Mark KOKORIS
发明人： C. Frederick BATTRELL , John GERDES , John R. WILLIFORD , Denise Maxine HOEKSTRA , Wayne L. BREIDFORD , Stephen MORDUE , John CLEMMENS , Melud NABAVI , Mark KOKORIS
IPC分类号： C12M1/34
CPC分类号： C12Q1/6834 , B01F11/0071 , B01F13/0059 , B01L3/5027 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/082 , B01L2200/10 , B01L2200/16 , B01L2300/0663 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , C12Q2563/131 , C12Q2525/197 , C12Q2563/143 , C12Q2565/629
摘要： Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.
摘要翻译： 公开了用于从临床样品进行核酸提取，扩增和检测的集成微流控柱。 这些设备是单进入，卫生和一次性的。 该装置能够进行单核或多重核酸靶标检测，例如：用于多种感染剂的测定板或癌细胞类型的测定板。 还公开了在全自动气动控制装置中使用微流控盒的方法。
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公开(公告)号：US20120164627A1
公开(公告)日：2012-06-28
申请号：US13342859
申请日：2012-01-03
申请人： C. Frederick Battrell , John Gerdes , Stephen Mordue , Jason Capodanno , Denise Maxine Hoekstra , John R. Williford , John Clemmens
发明人： C. Frederick Battrell , John Gerdes , Stephen Mordue , Jason Capodanno , Denise Maxine Hoekstra , John R. Williford , John Clemmens
IPC分类号： G01N33/53 , G01N33/569 , G01N21/75 , B01L3/00 , C12M1/40
CPC分类号： B01L3/50273 , B01F11/0071 , B01F11/0074 , B01F13/0059 , B01L3/502738 , B01L2300/0636 , B01L2300/0681 , B01L2300/069 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B01L2400/086 , F04B43/043 , F04B43/06 , G01N33/54366 , Y10T436/255
摘要： Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples.
摘要翻译： 公开了用于异质结合和凝集测定的微流体方法和装置，其中涉及在为临床测试样品的安全处理设计的系统中的混合和试剂和样品操作方面的改进。
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4.发明申请MICROFLUIDIC APPARATUS AND METHODS FOR PERFORMING BLOOD TYPING AND CROSSMATCHING 有权微血管装置及其方法，用于进行血液分型和交叉测量公开(公告)号：US20100112723A1
公开(公告)日：2010-05-06
申请号：US12572968
申请日：2009-10-02
申请人： C. Frederick Battrell , Diane Wierzbicki , John Clemmens , Jason Capodanno , John R. Williford , Carolina Elmufdi , Isaac Sprague
发明人： C. Frederick Battrell , Diane Wierzbicki , John Clemmens , Jason Capodanno , John R. Williford , Carolina Elmufdi , Isaac Sprague
IPC分类号： G01N33/566 , G01N33/48 , G01N33/50 , B81B7/00
CPC分类号： G01N33/53 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2300/0627 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B01L2300/161 , G01N33/5304 , G01N33/80
摘要： Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.
摘要翻译： 提供用于凝集反应的微流控盒。 药筒包括具有至少两个进气通道的微流体反应通道，一个用于含抗原的流体，另一个用于含抗体流体，结合到通过并入下游流动控制通道而修改的反应通道。 在低雷诺数下，两个输入流在反应通道中层叠在另一个之上，并形成流动的，未混合的水平分层的层状流体扩散（HLFD）界面，持续延长的反应时间。 令人惊奇的是，本发明的微流体回路的设计，表面性质和流动状态增强了在分层界面处的抗体介导的凝集的检测。 抗原：涉及通过这些装置增强的凝集的抗体反应可用于血型，输血交配和免疫诊断凝集试验。
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公开(公告)号：US20090325276A1
公开(公告)日：2009-12-31
申请号：US12411020
申请日：2009-03-25
申请人： C. Frederick Battrell , John Gerdes , Wayne L. Breidford , Jason Capodanno , Stephen Mordue , John Clemmens , Denise Maxine Hoekstra , Christy A. Lancaster , John R. Williford , Patrick Maloney , Joan Haab
发明人： C. Frederick Battrell , John Gerdes , Wayne L. Breidford , Jason Capodanno , Stephen Mordue , John Clemmens , Denise Maxine Hoekstra , Christy A. Lancaster , John R. Williford , Patrick Maloney , Joan Haab
IPC分类号： C12M1/00
CPC分类号： G01N33/5302 , B01F11/0071 , B01F13/0059 , B01L3/502715 , B01L7/52 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0864 , B01L2400/0481 , B01L2400/0487 , B01L2400/0638 , Y02A50/58
摘要： Combinations of microfluidic diagnostic testing modules for simultaneous evaluations of serological and molecular biological targets are provided, and include panel testing for both antibodies (or antigens) and nucleic acid targets in one single-use device. These improvements are directed to evaluating the overall progress and activity of a pathogenic process in real time, at the point of care, not merely the presence or absence of a particular diagnostic marker, which can often be incomplete or misleading.
摘要翻译： 提供了用于同时评估血清学和分子生物学靶标的微流体诊断测试模块的组合，并且包括在一次性使用的装置中对抗体（或抗原）和核酸靶标进行面板测试。 这些改进旨在评估致病过程的总体进展和活动，而不仅仅是特定诊断标记的存在或不存在，这通常是不完整的或具有误导性的。
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6.发明授权Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices 有权微流体装置中载体试剂脱水储存的组合物和方法公开(公告)号：US08921085B2
公开(公告)日：2014-12-30
申请号：US13323684
申请日：2011-12-12
申请人： C. Frederick Battrell , Denise Maxine Hoekstra , Joan Haab , John R. Williford , Sabrina N. Gates , Elizabeth R. Leber , Isaac Sprague
发明人： C. Frederick Battrell , Denise Maxine Hoekstra , Joan Haab , John R. Williford , Sabrina N. Gates , Elizabeth R. Leber , Isaac Sprague
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q2527/125
摘要： Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.
摘要翻译： 描述制造方法和组合物用于生产具有用于分子生物学测试的载体试剂的自含微流控盒装置。 敏感试剂以干燥形式储存而不进行冻干或冷冻，并在使用时与生物样品或样品洗脱液在使用时重构。 制造方法包括片和卷制造方法，其中将试剂印刷在适当位置并在凝胶玻璃化之前在单个微流体盒内密封。
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7.发明授权Rehydratable matrices for dry storage of TAQ polymerase in a microfluidic device 有权用于在微流体装置中干燥储存TAQ聚合酶的可再水化基质公开(公告)号：US08835146B2
公开(公告)日：2014-09-16
申请号：US13323675
申请日：2011-12-12
CPC分类号： C12N9/96 , B01L3/5027 , B01L2200/16 , C12N9/1252
摘要： Formulations for dry storage of PCR reagents are described. These formulations find use in manufacture of self-contained microfluidic card devices for PCR clinical testing in which the reagents are reconstituted at the point of testing. In these cards, TAQ polymerase is stored “on-board” in vitrified dry form without lyophilization or freezing, and is reconstituted by either the sample or a sample eluate during the assay.
摘要翻译： 描述了PCR试剂的干燥储存制剂。 这些配方可用于制造用于PCR临床测试的自给自足的微流控卡装置，其中试剂在测试点重构。 在这些卡中，TAQ聚合酶以玻璃化干燥形式储存在板上而不进行冻干或冷冻，并且在测定期间由样品或样品洗脱液重构。
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公开(公告)号：US08222023B2
公开(公告)日：2012-07-17
申请号：US12207627
申请日：2008-09-10
申请人： C. Frederick Battrell , John Gerdes , John R. Williford , Denise Maxine Hoekstra , Wayne L. Breidford , Stephen Mordue , John Clemmens , Melud Nabavi , Mark Kokoris
发明人： C. Frederick Battrell , John Gerdes , John R. Williford , Denise Maxine Hoekstra , Wayne L. Breidford , Stephen Mordue , John Clemmens , Melud Nabavi , Mark Kokoris
IPC分类号： C12M1/34
CPC分类号： C12Q1/6834 , B01F11/0071 , B01F13/0059 , B01L3/5027 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/082 , B01L2200/10 , B01L2200/16 , B01L2300/0663 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , C12Q2563/131 , C12Q2525/197 , C12Q2563/143 , C12Q2565/629
摘要： Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.
摘要翻译： 公开了用于从临床样品进行核酸提取，扩增和检测的集成微流控柱。 这些设备是单进入，卫生和一次性的。 该装置能够进行单核或多重核酸靶标检测，例如：用于多种感染剂的测定板或癌细胞类型的测定板。 还公开了在全自动气动控制装置中使用微流控盒的方法。
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公开(公告)号：US20090148933A1
公开(公告)日：2009-06-11
申请号：US12207627
申请日：2008-09-10
申请人： C. Frederick Battrell , John Gerdes , John R. Williford , Denise Maxine Hoekstra , Wayne L. Breidford , Stephen Mordue , John Clemmens , Melud Nabavi , Mark Kokoris
发明人： C. Frederick Battrell , John Gerdes , John R. Williford , Denise Maxine Hoekstra , Wayne L. Breidford , Stephen Mordue , John Clemmens , Melud Nabavi , Mark Kokoris
IPC分类号： C12M1/34
CPC分类号： C12Q1/6834 , B01F11/0071 , B01F13/0059 , B01L3/5027 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/082 , B01L2200/10 , B01L2200/16 , B01L2300/0663 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , C12Q2563/131 , C12Q2525/197 , C12Q2563/143 , C12Q2565/629
摘要： Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.
摘要翻译： 公开了用于从临床样品进行核酸提取，扩增和检测的集成微流控柱。 这些设备是单进入，卫生和一次性的。 该装置能够进行单核或多重核酸靶标检测，例如：用于多种感染剂的测定板或癌细胞类型的测定板。 还公开了在全自动气动控制装置中使用微流控盒的方法。
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10.发明授权Microfluidic apparatus and methods for performing blood typing and crossmatching 有权用于进行血液分型和交配的微流体装置和方法公开(公告)号：US08318439B2
公开(公告)日：2012-11-27
申请号：US12572968
申请日：2009-10-02
申请人： C. Frederick Battrell , Diane Wierzbicki , John Clemmens , Jason Capodanno , John R. Williford , Carolina Elmufdi , Isaac Sprague
发明人： C. Frederick Battrell , Diane Wierzbicki , John Clemmens , Jason Capodanno , John R. Williford , Carolina Elmufdi , Isaac Sprague
IPC分类号： G01N33/53
CPC分类号： G01N33/53 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2300/0627 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B01L2300/161 , G01N33/5304 , G01N33/80
摘要： Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.
摘要翻译： 提供用于凝集反应的微流控盒。 药筒包括具有至少两个进气通道的微流体反应通道，一个用于含抗原的流体，另一个用于含抗体流体，结合到通过并入下游流动控制通道而修改的反应通道。 在低雷诺数下，两个输入流在反应通道中层叠在另一个之上，并形成流动的，未混合的水平分层的层状流体扩散（HLFD）界面，持续延长的反应时间。 令人惊奇的是，本发明的微流体回路的设计，表面性质和流动状态增强了在分层界面处的抗体介导的凝集的检测。 抗原：涉及通过这些装置增强的凝集的抗体反应可用于血型，输血交配和免疫诊断凝集试验。
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