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    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from gluconobacter oxydans T-100
    1.
    发明授权
    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from gluconobacter oxydans T-100 失效
    L-山梨糖脱氢酶和由氧化葡萄糖葡萄球菌T-100获得的新型L-山梨糖酮脱氢酶

    公开(公告)号：US06197562B1

    公开(公告)日：2001-03-06

    申请号：US09118317

    申请日：1998-07-17

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    IPC分类号： C12N904

    CPC分类号： C12N9/0006 ,  Y10S435/822

    摘要： A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase both derived from Gluconobacter oxydans T-100, a DNA which encodes the SDH and/or SNDH, an expression vector which contains the DNA, a host cell transformed by the expression vector and a process for producing the SDH and/or SNDH, which comprises culturing the host cell in a medium and recovering the SDH and/or SNDH from the resulting culture. The SDH and SNDH of the present invention are useful enzymes having preferable properties for the production of 2-keto-L-gulonic acid, as well as L-ascorbic acid. According to the production method of the present invention, the SDH and SNDH having such preferable properties can be produced in large amounts by genetic engineering.

    摘要翻译： 一种新型的L-山梨糖脱氢酶（SDH）和一种新型的L-山梨糖酮脱氢酶，它们都衍生自氧化葡糖杆菌T-100，一种编码SDH和/或SNDH的DNA，含有该DNA的表达载体，由 表达载体和产生SDH和/或SNDH的方法，其包括在培养基中培养宿主细胞并从所得培养物中回收SDH和/或SNDH。 本发明的SDH和SNDH是生产2-酮-L-古洛糖酸以及L-抗坏血酸的优选性质的有用的酶。 根据本发明的制造方法，可以通过遗传工程大量生产具有优选性能的SDH和SNDH。

    Method for producing 2-keto-L-gulonic acid
    4.
    发明授权
    Method for producing 2-keto-L-gulonic acid 失效
    2-酮-L-古洛糖酸的制备方法

    公开(公告)号：US5888786A

    公开(公告)日：1999-03-30

    申请号：US943285

    申请日：1997-10-03

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Hayashi

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Hayashi

    IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12P7/60 ,  C12N1/00 ,  C12N15/00

    CPC分类号： C12N9/0008 ,  C12N9/0006 ,  C12P7/60 ,  Y10S435/822

    摘要： An expression vector containing both a DNA encoding an L-sorbose dehydrogenase and a DNA encoding an L-sorbosone dehydrogenase; a transformant having an ability to produce 2-keto-L-gulonic acid (hereinafter 2KLGA) at high yields from D-sorbitol, which is prepared by transforming, with said expression vector, a microorganism capable of producing L-sorbose at high yields from D-sorbitol, which has no or low 2KLGA-decomposing activity or a host microorganism having, in addition to the above-mentioned properties, no or low L-idonic acid-producing activity; and a process for producing 2KLGA, which comprises culturing said transformant in a medium containing D-sorbitol. According to the present invention, 2KLGA useful for the production of L-ascorbic acid can be produced with ease and in larger amounts by a single operation of culture.

    摘要翻译： 含有编码L-山梨糖脱氢酶的DNA和编码L-山梨糖酮脱氢酶的DNA的表达载体; 具有从D-山梨醇以高产率生产2-酮-L-古洛糖酸（以下称为2KLGA）的能力的转化体，其通过用所述表达载体转化能以高产率生产L-山梨糖的微生物制备 没有或低的2KLGA分解活性的D-山梨糖醇或除了上述性质之外，没有或低的L-天冬糖酸生产活性的宿主微生物; 以及生产2KLGA的方法，其包括在含有D-山梨糖醇的培养基中培养所述转化体。 根据本发明，用于生产L-抗坏血酸的2KLGA可以通过单次的培养操作容易地和大量地生产。

    Method for producing 2-keto-L-gulonic acid
    10.
    发明授权
    Method for producing 2-keto-L-gulonic acid 失效
    2-酮-L-古洛糖酸的制备方法

    公开(公告)号：US5834263A

    公开(公告)日：1998-11-10

    申请号：US696834

    申请日：1996-09-24

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Hayashi

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Hayashi

    IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12P7/60 ,  C12N1/00 ,  C12N15/00

    CPC分类号： C12N9/0008 ,  C12N9/0006 ,  C12P7/60 ,  Y10S435/822

    摘要： An expression vector containing both a DNA encoding an L-sorbose dehydrogenase and a DNA encoding an L-sorbosone dehydrogenase; a transformant having an ability to produce 2-keto-L-gulonic acid (hereinafter 2KLGA) at high yields from D-sorbitol, which is prepared by transforming, with said expression vector, a microorganism capable of producing L-sorbose at high yields from D-sorbitol, which has no or low 2KLGA-decomposing activity or a host microorganism having, in addition to the above-mentioned properties, no or low L-idonic acid-producing activity; and a process for producing 2KLGA, which comprises culturing said transformant in a medium containing D-sorbitol. According to the present invention, 2KLGA useful for the production of L-ascorbic acid can be produced with ease and in larger amounts by a single operation of culture.

    摘要翻译： PCT No.PCT / JP95 / 00285 Sec。 371日期：1996年9月24日 102（e）1996年9月24日PCT提交1995年2月24日PCT公布。 WO95 / 23220 PCT出版物 日期1995年8月31日含有编码L-山梨糖脱氢酶的DNA和编码L-山梨糖酮脱氢酶的DNA的表达载体; 具有从D-山梨醇以高产率生产2-酮-L-古洛糖酸（以下称为2KLGA）的能力的转化体，其通过用所述表达载体转化能以高产率生产L-山梨糖的微生物制备 没有或低的2KLGA分解活性的D-山梨糖醇或除了上述性质之外，没有或低的L-天冬糖酸生产活性的宿主微生物; 以及生产2KLGA的方法，其包括在含有D-山梨糖醇的培养基中培养所述转化体。 根据本发明，用于生产L-抗坏血酸的2KLGA可以通过单次的培养操作容易地和大量地生产。