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    SCREENING METHODS FOR COMPOUNDS THAT MODULATE THE ACTIVITY OF G-PROTEIN COUPLED RECEPTORS
    1.
    发明申请
    SCREENING METHODS FOR COMPOUNDS THAT MODULATE THE ACTIVITY OF G-PROTEIN COUPLED RECEPTORS 审中-公开
    调节G蛋白偶联受体活性的化合物的筛选方法

    公开(公告)号：US20130210046A1

    公开(公告)日：2013-08-15

    申请号：US13829318

    申请日：2013-03-14

    申请人： Nutrinova Nutrition Specialties & Food Ingredients GmbH

    发明人： Michael KROHN ,  Holger Zinke

    IPC分类号： C12Q1/02

    CPC分类号： C12Q1/025 ,  C12N15/79 ,  G01N33/566 ,  G01N2333/705 ,  G01N2500/10

    摘要： The present invention relates to a screening system for modulators of GPCRs. Further it relates to recombinant vector systems for the heterologous expression of heterodimeric G-protein coupled receptors (GPCRs) in eukaryotic host cells. Preferably the functional expression of engineered GPCRs for the perception of sweet and L-amino acid taste or more preferably the use of these receptors for the identification of functional ligands is also encompassed.

    摘要翻译： 本发明涉及一种用于GPCR调制器的筛选系统。 此外，它涉及异源二聚体G蛋白偶联受体（GPCR）在真核宿主细胞中异源表达的重组载体系统。 优选地，还包括用于感知甜和L-氨基酸味道的工程化GPCR的功能性表达或更优选地使用这些受体用于鉴定功能性配体。

    Process for producing acesulfame potassium
    2.
    发明授权
    Process for producing acesulfame potassium 有权
    生产乙酰磺胺酸钾的方法

    公开(公告)号：US09024016B2

    公开(公告)日：2015-05-05

    申请号：US13901735

    申请日：2013-05-24

    申请人： Nutrinova Nutrition Specialties & Food Ingredients GmbH

    发明人： Michael J. Bayer ,  Stephan Brietzke ,  Peter Groer ,  Christoph Mollenkopf

    IPC分类号： C07D291/06 ,  C07D307/02 ,  A23L1/236 ,  C07C307/02

    CPC分类号： A23L1/236 ,  A23L27/30 ,  C07C307/02 ,  C07D291/06

    摘要： In one embodiment, the invention relates to processes for producing acesulfame potassium. In one embodiment, the process comprises the step of reacting a first reaction mixture to form an amidosulfamic acid salt such as a trialkyl ammonium amidosulfamic acid salt. The first reaction mixture comprises sulfamic acid, an amine, and smaller amounts, if any, acetic acid, e.g., less than 1 wt % (10000 wppm). In terms of ranges, the first reaction mixture may comprise from 1 wppm to 1 wt % acetic acid. The process further comprises the step of reacting the amidosulfamic acid salt with diketene to form an acetoacetamide salt. In preferred embodiments, the amidosulfamic acid salt formation reaction is conducted at pH levels from 5.5 to 7.0. The process further comprises the step of deriving the acesulfame-K from the acetoacetamide salt.

    摘要翻译： 在一个实施方案中，本发明涉及生产乙酰磺胺酸钾的方法。 在一个实施方案中，该方法包括使第一反应混合物反应形成氨基磺酰胺酸盐如氨基磺酰胺酸三烷基铵盐的步骤。 第一反应混合物包含氨基磺酸，胺和少量（如果有的话）乙酸，例如小于1重量％（10000wppm））。 就范围而言，第一反应混合物可以包含1wppm至1wt％的乙酸。 该方法还包括使氨基磺酰胺酸盐与双烯酮反应形成乙酰乙酰胺盐的步骤。 在优选的实施方案中，氨基磺酰胺酸盐形成反应在5.5至7.0的pH水平下进行。 该方法还包括从乙酰乙酰胺盐衍生乙酰磺胺酸K的步骤。

    METHOD FOR THE CULTIVATION OF MICROORGANISMS OF THE GENUS THRAUSTOCHYTRIALES BY USING AN OPTIMIZED LOW SALT MEDIUM
    4.
    发明申请
    METHOD FOR THE CULTIVATION OF MICROORGANISMS OF THE GENUS THRAUSTOCHYTRIALES BY USING AN OPTIMIZED LOW SALT MEDIUM 审中-公开
    通过使用优化的低盐培养基来培养基因组织微生物的方法

    公开(公告)号：US20150104557A1

    公开(公告)日：2015-04-16

    申请号：US14525315

    申请日：2014-10-28

    申请人： Nutrinova Nutrition Specialties & Food Ingredients GMBH

    发明人： Matthias Rusing ,  Markus Luy

    IPC分类号： C12P7/64 ,  A23K1/14 ,  A23L1/30

    CPC分类号： C12P7/6427 ,  A23K10/37 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/12 ,  C12P7/6472

    摘要： The invention relates to an optimized method for cultivating microorganisms of the genus Thraustochytriales, according to which the microorganisms are cultivated in a low salt medium without adding sodium salts and chloride salts, the total salt content being less than 3.5 g/L (corresponding to less than 10 percent of sea water salt content), whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention especially relates to novel optimized media having a substantially reduced total salt content, above all a particularly reduced NaCl content. The production of PUFAs can be substantially improved and significantly simplified by using a novel combination of different salts as a media composition in which the overall weight ratios of ions do not exceed 1.75 g/L. Furthermore, said medium preferably contains no added sodium salt and chloride salt at all, which helps prevent environmental damages caused by wastewaters containing salt.

    摘要翻译： 本发明涉及一种用于培养破囊壶菌属微生物的优化方法，根据该方法，微生物在低盐培养基中培养而不添加钠盐和氯化物盐，总盐含量小于3.5g / L（相当于少于 超过10％的海水盐含量），由此从微生物和/或介质中分离PUFA。 本发明特别涉及具有显着降低的总盐含量，特别是特别降低的NaCl含量的新型优化的介质。 通过使用不同盐的新型组合作为其中离子的总重量比不超过1.75g / L的介质组合物，可以显着改善和显着地简化PUFA的生产。 此外，所述介质优选根本不含有添加的钠盐和氯化物盐，这有助于防止由含有盐的废水引起的环境损害。

    Expression of recombinant human proteins in Tetrahymena
    5.
    发明申请
    Expression of recombinant human proteins in Tetrahymena 有权
    重组人蛋白在四膜虫中的表达

    公开(公告)号：US20030219869A1

    公开(公告)日：2003-11-27

    申请号：US10395433

    申请日：2003-03-24

    申请人： NUTRINOVA Nutrition Specialties & Food Ingredients GmbH

    发明人： Thomas Kiy ,  Matthias Rusing

    IPC分类号： C12P021/02 ,  C12N001/10 ,  C12N015/74

    CPC分类号： C07K14/765 ,  C12N15/79

    摘要： The present invention concerns a method for production of recombinant human proteins, in which Tetrahymena cells are transformed with recombinant DNA containing at least one functional gene that codes for a human protein, the recombinant Tetrahymena cells are cultured, in which the gene that codes for a human protein is expressed and the proteins are then isolated. The present invention also concerns a corresponding method, in which the gene that codes for a human protein contains a human leader sequence that leads to secretion of the expressed protein.

    摘要翻译： 本发明涉及重组人蛋白质的制备方法，其中用包含编码人蛋白质的至少一个功能基因的重组DNA转化四膜虫细胞，培养重组四膜虫细胞，其中编码 表达人蛋白质，然后分离蛋白质。 本发明还涉及一种相应的方法，其中编码人蛋白质的基因含有导致表达的蛋白质分泌的人前导序列。