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    Host organism capture
    6.
    发明授权
    Host organism capture 失效
    宿主有机体捕获

    公开(公告)号：US5750346A

    公开(公告)日：1998-05-12

    申请号：US695834

    申请日：1996-08-07

    申请人： John A. Bridgham ,  John Brandis ,  John Leong ,  Paul D. Hoeprich, Jr. ,  Charles L. Sloan ,  Roger A. O'Neill ,  Charles Andre

    发明人： John A. Bridgham ,  John Brandis ,  John Leong ,  Paul D. Hoeprich, Jr. ,  Charles L. Sloan ,  Roger A. O'Neill ,  Charles Andre

    IPC分类号： C12N15/10 ,  C12N15/63 ,  C12N15/70 ,  C12P21/04 ,  C40B40/02 ,  C12Q1/68 ,  G01N33/483 ,  G01N33/53 ,  C07N21/04

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12N15/635 ,  C12N15/70

    摘要： Cloning systems useful for the isolation of recombinant nucleic acid are disclosed in which the recombination of cloning-system nucleic acid and foreign nucleic acid is linked to the expression of a moiety on the surface of a host organism, the moiety being a first member of a binding pair. When recombination occurs between the nucleic acid and the foreign nucleic acid, the moiety is expressed on the surface of the host organism. The isolation of recombinant nucleic acid is then performed by attaching a second member of the binding pair to a solid support and contacting the host organism with the support. When the first member of the binding pair is expressed on the surface of the host organism, the host organism binds to the second member of the binding pair attached to the solid support, thereby selectively isolating those organisms. Other aspects of the invention include devices for the isolation of individual cells that differentially express binding moieties on their surface. The devices may be adapted for polynucleotide sequencing and analysis of polynucleotides in the isolated cells. Additional aspects of the invention include methods of using the devices for single cell isolation and analysis.

    摘要翻译： 公开了用于分离重组核酸的克隆系统，其中克隆系统核酸和外来核酸的重组与宿主生物表面上的部分的表达相关，该部分是第一成员 绑定对。 当在核酸和外来核酸之间发生重组时，该部分在宿主生物体的表面上表达。 然后通过将结合对的第二个成员连接到固体支持物并使宿主生物与载体接触来进行重组核酸的分离。 当结合对的第一个成员在宿主生物的表面上表达时，宿主生物体结合到与固体支持物连接的结合对的第二个成员，从而选择性分离那些生物。 本发明的其它方面包括用于分离在其表面上差异表达结合部分的各个细胞的装置。 所述装置可以适用于分离的细胞中的多核苷酸的多核苷酸测序和分析。 本发明的另外的方面包括使用该装置进行单细胞隔离和分析的方法。