Chemical arrays
    1.
    发明授权
    Chemical arrays 失效
    化学阵列

    公开(公告)号:US07300798B2

    公开(公告)日:2007-11-27

    申请号:US10167662

    申请日:2001-10-18

    IPC分类号: G01N30/00

    摘要: A method and apparatus for using arrays of polymers each having a pattern of features over a corresponding array region on a surface of a flexible elongated web. In this method each array region may be exposed to a corresponding continuous volume of a sample fluid. A method and apparatus are also provided for reading arrays disposed along a surface of a flexible elongated web. In one such method the web with the arrays thereon may be transported in a lengthwise direction past a reading location at which a characteristic of the features is read, while restraining the web on both surfaces on either side of the reading location to assist in maintaining the reading location flat.

    摘要翻译: 一种使用聚合物阵列的方法和装置,每个聚合物阵列具有在柔性细长网的表面上的相应阵列区域上的特征图案。 在该方法中,每个阵列区域可以暴露于相应的连续体积的样品流体。 还提供了一种用于读取沿着柔性细长网的表面设置的阵列的方法和装置。 在一种这样的方法中,其上具有阵列的幅材可以沿长度方向传送通过读取特征的特征的读取位置,同时在读取位置的任一侧的两个表面上限制纸幅以帮助维持 阅读位置平坦。

    Manufacture of arrays with varying deposition parameters
    3.
    发明授权
    Manufacture of arrays with varying deposition parameters 失效
    制造具有不同沉积参数的阵列

    公开(公告)号:US07078505B2

    公开(公告)日:2006-07-18

    申请号:US10165783

    申请日:2002-06-06

    IPC分类号: C07H21/04 C12Q1/68 C12M1/36

    摘要: Methods are disclosed for synthesizing a plurality of compounds on the surface of supports. The synthesis comprises a series of cycles of steps in which reagents for conducting the synthesis are deposited on the surface of the support to form the chemical compounds. At least one physical parameter of the deposition varies between cycles The method comprises conducting the synthesis in at least two sets of cycles, arbitrarily designated as a first set and a second set. Each cycle comprises at least one step of depositing reagents on a surface by means of droplet dispensing elements that traverse the surface of the support. At least one, and desirably all, of the following deposition parameters are employed as indicated: The number of times of repetition for a step of depositing reagents in at least one cycle of the second set is less than the number of times of repetition for a corresponding step in at least one cycle of the first set. The rate of deposition in at least one cycle of the second set is slower than or equal to the rate of deposition in at least one cycle of the first set. The rate of traversal of the dispensing elements over the surface of the support in at least one cycle of the second set is greater than such rate of traversal in at least one cycle of the first set.

    摘要翻译: 公开了在载体表面上合成多种化合物的方法。 该合成包括一系列循环的步骤,其中用于进行合成的试剂沉积在载体的表面上以形成化合物。 沉积物的至少一个物理参数在循环之间变化。该方法包括以任意指定为第一组和第二组的至少两组循环进行合成。 每个循环包括至少一个步骤,其通过穿过载体表面的液滴分配元件在表面上沉积试剂。 如下所示使用以下沉积参数中的至少一个,期望地全部以下沉积参数:在第二组的至少一个循环中沉积试剂的步骤的重复次数小于第二组的重复次数 在第一组的至少一个循环中的对应步骤。 在第二组的至少一个循环中的沉积速率慢于或等于第一组的至少一个循环中的沉积速率。 在第二组的至少一个循环中,分配元件在支撑体表面上的遍历速率大于在第一组的至少一个循环中的穿过速率。

    Oligonucleotide probes for detection of periodontal pathogens
    6.
    发明授权
    Oligonucleotide probes for detection of periodontal pathogens 失效
    用于检测牙周病原体的寡核苷酸探针

    公开(公告)号:US5378604A

    公开(公告)日:1995-01-03

    申请号:US3367

    申请日:1993-01-12

    CPC分类号: C12Q1/689

    摘要: This invention relates to compositions of oligonucleotide probes for use in the detection of bacteria associated with medical disorders of the human mouth, wherein said probes consist essentially of a segment of nucleic acid capable of selectively hybridizing under hybridizing conditions, to the 16S or 23S ribosomal RNA [rRNA] of said bacteria. Methods for detection, as well as diagnostic kits for the assay of these bacterium, are also disclosed.

    摘要翻译: 本发明涉及用于检测与人口的医学病症相关的细菌的寡核苷酸探针的组合物,其中所述探针基本上由能够在杂交条件下选择性杂交的核酸片段与16S或23S核糖体RNA 所述细菌的[rRNA]。 还公开了检测方法以及用于这些细菌测定的诊断试剂盒。

    Oligonucleotide probes for the detection of periodontal pathogens
    8.
    发明授权
    Oligonucleotide probes for the detection of periodontal pathogens 失效
    用于检测牙周病原体的寡核苷酸探针

    公开(公告)号:US5212059A

    公开(公告)日:1993-05-18

    申请号:US571563

    申请日:1990-08-29

    IPC分类号: C12Q1/68

    CPC分类号: C12Q1/689

    摘要: This invention relates to compositions of oligonucleotide probes for use in the detection of bacteria associated with medical disorders of the human mouth, wherein said probes consist essentially of a segment of nucleic acid capable of selectively hybridizing under hybridizing conditions, to the 16S or 23S ribosomal RNA [rRNA] of said bacteria. Methods for detection, as well as diagnostic kits for the assay of these bacterium, are also disclosed.

    摘要翻译: PCT No.PCT / US89 / 00072 Sec。 371日期1990年8月29日第 102(e)1990年8月29日的PCT日期1989年1月9日提交PCT本发明涉及用于检测与人口的医学病症相关的细菌的寡核苷酸探针的组合物,其中所述探针基本上由 的能够在杂交条件下选择性杂交的核酸与所述细菌的16S或23S核糖体RNA [rRNA]。 还公开了检测方法以及用于这些细菌测定的诊断试剂盒。

    Minimization of blooming in high-density arrays by using reactive wash reagents
    9.
    发明授权
    Minimization of blooming in high-density arrays by using reactive wash reagents 失效
    通过使用反应性洗涤试剂在高密度阵列中最小化起霜

    公开(公告)号:US06184347B2

    公开(公告)日:2001-02-06

    申请号:US09195869

    申请日:1998-11-19

    IPC分类号: A61K3804

    摘要: A wash reagent employed for the bulk washing of the surface of a high-density array to remove unreacted reactants from cells of the array while, at the same time, reacting with the unreacted monomer in order to prevent reaction of the reacted monomer with functional groups on the surface of the HDA outside of the region of the surface to which the reactive monomer is applied. The wash reagent is chosen for a particular solid-state synthesis so that the unreacted reactants and catalyzing agents are soluble in the wash reagent, so that the wash reagent does not react with, or catalyze, reactions of the substrate or the biopolymers bound to the substrate, and so that the wash reagent reacts with unreacted reactive monomer in order to prevent subsequent reactions of the unreacted reactive monomer.

    摘要翻译: 用于大量洗涤高密度阵列表面的洗涤剂,以从阵列的细胞中除去未反应的反应物,同时与未反应的单体反应,以防止反应的单体与官能团的反应 在HDA的表面上,与表面反应性单体所应用的区域之外。 选择洗涤试剂用于特定的固态合成,使得未反应的反应物和催化剂可溶于洗涤试剂中,使得洗涤试剂不与底物或与生物聚合物结合的生物聚合物反应或催化反应 底物,并且使得洗涤剂与未反应的反应性单体反应,以防止未反应的反应性单体随后的反应。