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12 条记录 (耗时 0.041 秒)

    Manufacture of arrays with varying deposition parameters
    1.
    发明授权
    Manufacture of arrays with varying deposition parameters 失效
    制造具有不同沉积参数的阵列

    公开(公告)号：US07078505B2

    公开(公告)日：2006-07-18

    申请号：US10165783

    申请日：2002-06-06

    申请人： Jay K. Bass ,  Maryam Mobed-Miremadi ,  Michelle M. Maranowski ,  Roy H. Kanemoto ,  Bill J. Peck ,  Eric M. Leproust ,  Michael P. Caren

    发明人： Jay K. Bass ,  Maryam Mobed-Miremadi ,  Michelle M. Maranowski ,  Roy H. Kanemoto ,  Bill J. Peck ,  Eric M. Leproust ,  Michael P. Caren

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12M1/36

    CPC分类号： C03C17/32 ,  B01J2219/00286 ,  B01J2219/00378 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00698 ,  B01J2219/00722 ,  B01L3/0241 ,  C03C17/002 ,  C40B40/06 ,  C40B60/14

    摘要： Methods are disclosed for synthesizing a plurality of compounds on the surface of supports. The synthesis comprises a series of cycles of steps in which reagents for conducting the synthesis are deposited on the surface of the support to form the chemical compounds. At least one physical parameter of the deposition varies between cycles The method comprises conducting the synthesis in at least two sets of cycles, arbitrarily designated as a first set and a second set. Each cycle comprises at least one step of depositing reagents on a surface by means of droplet dispensing elements that traverse the surface of the support. At least one, and desirably all, of the following deposition parameters are employed as indicated: The number of times of repetition for a step of depositing reagents in at least one cycle of the second set is less than the number of times of repetition for a corresponding step in at least one cycle of the first set. The rate of deposition in at least one cycle of the second set is slower than or equal to the rate of deposition in at least one cycle of the first set. The rate of traversal of the dispensing elements over the surface of the support in at least one cycle of the second set is greater than such rate of traversal in at least one cycle of the first set.

    摘要翻译： 公开了在载体表面上合成多种化合物的方法。 该合成包括一系列循环的步骤，其中用于进行合成的试剂沉积在载体的表面上以形成化合物。 沉积物的至少一个物理参数在循环之间变化。该方法包括以任意指定为第一组和第二组的至少两组循环进行合成。 每个循环包括至少一个步骤，其通过穿过载体表面的液滴分配元件在表面上沉积试剂。 如下所示使用以下沉积参数中的至少一个，期望地全部以下沉积参数：在第二组的至少一个循环中沉积试剂的步骤的重复次数小于第二组的重复次数 在第一组的至少一个循环中的对应步骤。 在第二组的至少一个循环中的沉积速率慢于或等于第一组的至少一个循环中的沉积速率。 在第二组的至少一个循环中，分配元件在支撑体表面上的遍历速率大于在第一组的至少一个循环中的穿过速率。

    Preparation of biopolymer arrays
    3.
    发明申请
    Preparation of biopolymer arrays 审中-公开
    生物聚合物阵列的制备

    公开(公告)号：US20080085511A1

    公开(公告)日：2008-04-10

    申请号：US11543369

    申请日：2006-10-05

    申请人： Bill J. Peck ,  Eric M. Leproust

    发明人： Bill J. Peck ,  Eric M. Leproust

    IPC分类号： C12Q1/68 ,  B41J2/01 ,  G06F19/00 ,  C12M3/00

    CPC分类号： C07K1/047 ,  B01J19/0046 ,  B01J2219/0036 ,  B01J2219/00378 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B82Y30/00

    摘要： Methods and apparatus are disclosed for preparing an array of biopolymer features on a substrate by initiating multiple cycles of drop deposition of biopolymer forming reagents to feature locations on the surface of the substrate. Each cycle comprises at least one step of deposition of biopolymer monomers or biopolymer subunits. For at least one of the biopolymer forming reagents and for at least a portion of the method, a dispensing protocol is employed wherein the biopolymer forming reagent is dispensed from a separate drop dispenser of a drop dispensing device and each drop dispenser comprises multiple nozzles. Multiple passes of the drop dispensing devices are employed for each cycle. In each cycle of the dispensing protocol drops of reagents are dispensed to feature locations from at least two different nozzles for each reagent in at least two different passes of a drop dispensing device. The cycles are repeated a sufficient number of times to prepare the array of biopolymer features.

    摘要翻译： 公开了用于通过引发多个循环的生物聚合物形成试剂的滴加沉积在基底表面上的特征位置来制备基底上生物聚合物特征阵列的方法和装置。 每个循环包括沉积生物聚合物单体或生物聚合物亚单元的至少一个步骤。 对于生物聚合物形成试剂和至少一部分方法中的至少一种，使用分配方案，其中生物聚合物形成试剂从滴剂分配装置的单独的液滴分配器分配，并且每个滴液分配器包括多个喷嘴。 每个循环采用多次滴液分配装置。 在分配方案的每个循环中，在液滴分配装置的至少两个不同通道中，每个试剂滴从多个不同喷嘴的至少两个不同喷嘴分配到特征位置。 循环重复足够次数以制备生物聚合物特征阵列。

    In Situ Nucleic Acid Array Synthesis Compositions
    7.
    发明申请
    In Situ Nucleic Acid Array Synthesis Compositions 审中-公开
    原位核酸阵列合成组合物

    公开(公告)号：US20090325821A1

    公开(公告)日：2009-12-31

    申请号：US12340796

    申请日：2008-12-22

    申请人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    发明人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    IPC分类号： C40B50/18 ,  C40B60/14

    CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00527

    摘要： In situ nucleic acid synthesis compositions are provided. The compositions include a solvent; and at least one of: a viscosity modifier; and a surface tension modifier. During use, the compositions further include one of a phosphoramidite and an activator. Also provide are methods of using the compositions, e.g., in the synthesis of nucleic acid arrays, and systems kits for practicing the methods.

    摘要翻译： 提供原位核酸合成组合物。 组合物包括溶剂; 和至少一种：粘度调节剂; 和表面张力调节剂。 在使用期间，组合物还包括亚磷酰胺和活化剂之一。 还提供使用组合物的方法，例如在核酸阵列的合成中以及用于实施该方法的系统试剂盒。

    In situ nucleic acid array synthesis compositions
    8.
    发明申请
    In situ nucleic acid array synthesis compositions 审中-公开
    原位核酸阵列合成组合物

    公开(公告)号：US20080085513A1

    公开(公告)日：2008-04-10

    申请号：US11545931

    申请日：2006-10-10

    申请人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    发明人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00527

    摘要： In situ nucleic acid synthesis compositions are provided. The compositions include a solvent; and at least one of: a viscosity modifier; and a surface tension modifier. During use, the compositions further include one of a phosphoramidite and an activator. Also provide are methods of using the compositions, e.g., in the synthesis of nucleic acid arrays, and systems kits for practicing the methods.

    摘要翻译： 提供原位核酸合成组合物。 组合物包括溶剂; 和至少一种：粘度调节剂; 和表面张力调节剂。 在使用期间，组合物还包括亚磷酰胺和活化剂之一。 还提供使用组合物的方法，例如在核酸阵列的合成中以及用于实施该方法的系统试剂盒。

    Flow cell humidity sensor system
    9.
    发明授权
    Flow cell humidity sensor system 失效
    流量计湿度传感器系统

    公开(公告)号：US06838888B2

    公开(公告)日：2005-01-04

    申请号：US10017336

    申请日：2001-12-13

    申请人： Bill J. Peck

    发明人： Bill J. Peck

    IPC分类号： G01N27/22 ,  G01R27/26 ,  G01N33/543

    CPC分类号： G01N27/225

    摘要： A system for monitoring water concentration in gaseous sample is disclosed. It is readily applicable in the synthesis of biopolymer arrays/microarrays involving the use of water-sensitive reagents. A flow cell is provided in which a capacitance sensor is placed separate from a production or synthesis environment. Sample and dry gas may be provided to the flow cell via conduits and valves or a manifold system. Dry gas, such as N2, is used to dry the sensor or simply to maintain it in a dried condition. The same gas may be used to drive an optional venturi pump to draw sample for measurement into the cell from the synthesis environment.