公开(公告)号：US06359125B1

公开(公告)日：2002-03-19

申请号：US09762611

申请日：2001-02-07

申请人： Min-Hwan Kim ,  Do-Yun Kim ,  Bong-Seok Moon ,  Jae-Chan Park ,  Young-Hee Kim ,  Seung-Joo Seo

发明人： Min-Hwan Kim ,  Do-Yun Kim ,  Bong-Seok Moon ,  Jae-Chan Park ,  Young-Hee Kim ,  Seung-Joo Seo

IPC分类号： C12Q168

CPC分类号： B82Y30/00 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00711 ,  B01J2219/00729 ,  C07B2200/11 ,  C07K14/003 ,  C40B50/14 ,  C40B60/14 ,  Y02P20/55

摘要： The present invention relates to a process for preparing arrays of oligopeptide nucleic acid probes immobilized on a solid matrix by employing polymeric photoacid generator. Arrays of peptide nucleic acid probes of the invention are prepared by the steps of: (i) derivatizing the surface of a solid matrix with aminoalkyloxysilane in alcohol and attaching a linker with acid-labile protecting group on the solid matrix; (ii) coating the solid matrix with polymeric photoacid generator (PAG); (iii) exposing the solid matrix thus coated to light to generate acid for eliminating acid-labile protecting group; (iv) washing the solid matrix with alkaline solution or organic solvent and removing residual polymeric photoacid generator; and, (v) attaching a monomeric peptide nucleic acid with acid-labile protecting group to the solid matrix, and repeating the previous Steps of (ii) to (v). In accordance with the present invention, neutral peptide nucleic acid probes, as the promising substitute for conventional negatively-charged oligonucleotide probes, can be prepared by employing polymeric photoacid generator in a simple and efficient manner, while overcoming the problems confronted in the prior art DNA chip fabrication using PR system and PPA system.

摘要翻译： 本发明涉及通过使用聚合光致酸发生剂制备固定在固体基质上的寡肽核酸探针阵列的方法。 通过以下步骤制备本发明的肽核酸探针的阵列：（i）用醇中的氨基烷氧基硅烷将固体基质的表面衍生化，并在固体基质上连接具有酸不稳定保护基的连接体; （ii）用聚合物光酸发生剂（PAG）涂覆固体基质; （iii）将由此涂覆的固体基质曝光以产生酸以消除酸不稳定保护基团; （iv）用碱性溶液或有机溶剂洗涤固体基质，除去残留的聚合光致酸发生剂; 和（v）将具有酸不稳定保护基团的单体肽核酸连接到固体基质上，并重复（ii）至（v）的先前步骤。 根据本发明，作为常规带负电荷的寡核苷酸探针的前景替代物的中性肽核酸探针可以通过以简单有效的方式使用聚合光致酸发生剂来制备，同时克服现有技术DNA中所面临的问题 使用PR系统和PPA系统的芯片制造。

公开(公告)号：US06960425B2

公开(公告)日：2005-11-01

申请号：US10693647

申请日：2003-10-27

申请人： Myung Sup Jung ,  Jong Jin Park ,  Sung Ouk Jung ,  Seung Joo Seo ,  Bon Won Koo

发明人： Myung Sup Jung ,  Jong Jin Park ,  Sung Ouk Jung ,  Seung Joo Seo ,  Bon Won Koo

IPC分类号： B82B3/00 ,  B82Y30/00 ,  B82Y40/00 ,  C01B31/02 ,  G03F1/00 ,  G03F7/039 ,  G03F7/16 ,  G03F7/40 ,  H01B13/00 ,  G03F7/30

CPC分类号： B82Y30/00 ,  B82Y10/00 ,  G03F7/0392 ,  G03F7/165 ,  G03F7/168 ,  G03F7/40 ,  Y10S977/842

摘要： A method for forming a pattern of carbon nanotubes includes forming a pattern on a surface-treated substrate using a photolithographic process, and laminating carbon nanotubes thereon using a chemical self-assembly process so as to form the carbon nanotubes in a monolayer or multilayer structure. A monolayer or multilayer carbon nanotube pattern may be easily formed on the substrate, e.g., glass, a silicon wafer and a plastic. Accordingly, the method can be applied to form patterned carbon nanotube layers having a high conductivity, and thus will be usefully utilized in the manufacturing processes of energy storages, for example, solar cells and batteries, flat panel displays, transistors, chemical and biological sensors, semiconductor devices and the like.

摘要翻译： 形成碳纳米管图案的方法包括使用光刻工艺在表面处理的基板上形成图案，并使用化学自组装工艺在其上层压碳纳米管，以便以单层或多层结构形成碳纳米管。 单层或多层碳纳米管图案可以容易地形成在基底上，例如玻璃，硅晶片和塑料。 因此，该方法可以应用于形成具有高导电性的图案化碳纳米管层，因此将有用地用于能量存储器的制造过程中，例如太阳能电池和电池，平板显示器，晶体管，化学和生物传感器 ，半导体器件等。

公开(公告)号：US06660479B2

公开(公告)日：2003-12-09

申请号：US10073071

申请日：2002-02-07

申请人： Min-Hwan Kim ,  Do-Yun Kim ,  Bong-Seok Moon ,  Jae-Chan Park ,  Young-Hee Kim ,  Seung-Joo Seo

发明人： Min-Hwan Kim ,  Do-Yun Kim ,  Bong-Seok Moon ,  Jae-Chan Park ,  Young-Hee Kim ,  Seung-Joo Seo

IPC分类号： C12Q168

CPC分类号： B82Y30/00 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00711 ,  B01J2219/00729 ,  C07B2200/11 ,  C07K14/003 ,  C40B50/14 ,  C40B60/14 ,  Y02P20/55

摘要： Disclosed is a process for preparing arrays of oligopeptide nucleic acid probes immobilized on a solid matrix by employing polymeric photoacid generator. Arrays of peptide nucleic acid probes of the invention are prepared by the steps of: (i) derivatizing the surface of a solid matrix with aminoalkyloxysilane in alcohol and attaching a linker with acid-labile protecting group on the solid matrix; (ii) coating the solid matrix with polymeric photoacid generator(PAG); (iii) exposing the solid matrix thus coated to light to generate acid for eliminating acid-labile protecting group; (iv) washing the solid matrix with alkaline solution or organic solvent and removing residual polymeric photoacid generator; and, (v) attaching a monomeric peptide nucleic acid with acid-labile protecting group to the solid matrix, and repeating the previous Steps of (ii) to (v). In accordance with the present invention, neutral peptide nucleic acid probes, as the promising substitute for conventional negatively-charged oligonucleotide probes, can be prepared by employing polymeric photoacid generator in a simple and efficient manner, while overcoming the problems confronted in the prior art DNA chip fabrication using PR system and PPA system.

公开(公告)号：US5889067A

公开(公告)日：1999-03-30

申请号：US65693

申请日：1998-04-24

申请人： Mi-seon Jang ,  Jin-taek Hwang ,  Seung-joo Seo

发明人： Mi-seon Jang ,  Jin-taek Hwang ,  Seung-joo Seo

IPC分类号： F25D23/06 ,  C08G18/00 ,  C08G18/28 ,  C08G101/00 ,  C08J9/04 ,  C08J9/14 ,  F25D23/08 ,  C08G18/30 ,  C08G18/32

CPC分类号： C08J9/148 ,  C08G18/282 ,  C08J9/146 ,  C08G2101/0025 ,  C08G2101/0058 ,  C08G2105/02 ,  C08G2330/50 ,  C08J2375/04

摘要： Disclosed is a method for producing an open cell rigid polyurethane foam which comprises reacting the mixture of a polyol for producing rigid polyurethane foam, a volatile blowing agent which is free from environmental pollution and selected from the group consisting of hydrocarbons, hydrofluoroalkanes and perfluoroalkanes or a mixture of the volatile blowing agent with water, a monohydric fatty alcohol having a good solubility to hydrocarbon as a cell opening agent, a foam stabilizer forming a very small cell in the presence of a isocyanate trimer catalyst and an organic isocyanate. The resultant open cell rigid polyurethane foam has a cell size of about less than 95 um, and is suitable for use, for example, as a core material in a vacuum heat insulating material. The foam may be enclosed in a container under a vacuum of 0.1.about.0.01 torr and has a thermal conductivity of 0.0045.about.0.0050 kcal/mhr.degree.C.

摘要翻译： 本发明公开了一种开孔硬质聚氨酯泡沫的制造方法，其特征在于，使硬质聚氨酯泡沫塑料用多元醇的混合物，不含环境污染的挥发性发泡剂，选自碳氢化合物，氢氟烷烃，全氟烷烃，或 挥发性发泡剂与水的混合物，对烃类作为开孔剂具有良好溶解性的一元脂肪醇，在异氰酸酯三聚体催化剂和有机异氰酸酯的存在下形成非常小的电池的泡沫稳定剂。 所得的开孔硬质聚氨酯泡沫具有小于95um的电池尺寸，并且适用于例如真空绝热材料中的芯材料。 泡沫可以在0.1分差为0.01托的真空下封闭在容器中，并具有0.0045分辨率0.0050千卡/米尔℃的热导率。