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    METHODS FOR DETECTING AND ANALYSIS OF PROTEINS
    1.
    发明公开
    METHODS FOR DETECTING AND ANALYSIS OF PROTEINS 审中-公开

    公开(公告)号：US20240327902A1

    公开(公告)日：2024-10-03

    申请号：US18616941

    申请日：2024-03-26

    申请人： Shifeng LI

    发明人： Shifeng LI

    IPC分类号： C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876

    CPC分类号： C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876

    摘要： A method for detection and analysis of proteins includes the steps of: (a) providing a 1st capture molecule on 1st solid surfaces through a cleavable linker; (b) capturing a target protein with the 1st capture molecule on the 1st solid surfaces; (c) generating an attaching group on the captured protein without disrupting the captured protein complex; (d) cleaving the captured protein complex and immobilized on the 2nd solid surfaces through the attaching group on the captured protein; (e) releasing the 1 st capture molecule; (f) contacting the immobilized protein with 2nd capture molecule with a coding DNA tag; (g) transferring the DNA barcode in the coding DNA tag to a primer on the 2nd solid surfaces; (h) optionally amplifying the extended coding DNA strand into a cluster in situ on the 2nd solid surfaces; and (i) decoding DNA strands on the 2nd solid surfaces.

    HIGH CAPACITANCE MICROWELL
    3.
    发明申请
    HIGH CAPACITANCE MICROWELL 有权
    高电容MICROWELL

    公开(公告)号：US20130189158A1

    公开(公告)日：2013-07-25

    申请号：US13354108

    申请日：2012-01-19

    申请人： Shifeng LI ,  James Bustillo ,  Wolfgang Hinz

    发明人： Shifeng LI ,  James Bustillo ,  Wolfgang Hinz

    IPC分类号： G01N27/00 ,  B05D3/00 ,  B05D5/12

    CPC分类号： G01N27/4145 ,  C12Q1/6869 ,  G01N27/414 ,  Y10T436/143333

    摘要： A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. The well wall structure defines an upper surface and defines a wall surface extending between the upper surface and the lower surface. The system further includes a conductive layer disposed over the lower surface and the wall surface.

    摘要翻译： 一种系统包括传感器，其包括传感器垫和井壁结构，其限定了良好地操作地耦合到传感器垫的结构。 该井由设置在传感器垫上的下表面进一步限定。 井壁结构限定了上表面并且限定了在上表面和下表面之间延伸的壁表面。 该系统还包括设置在下表面和壁表面上的导电层。

    Chemical sensor with conductive cup-shaped sensor surface
    4.
    发明授权
    Chemical sensor with conductive cup-shaped sensor surface 有权
    化学传感器带导电杯形传感器表面

    公开(公告)号：US08747748B2

    公开(公告)日：2014-06-10

    申请号：US13354108

    申请日：2012-01-19

    申请人： Shifeng Li ,  James Bustillo ,  Wolfgang Hinz

    发明人： Shifeng Li ,  James Bustillo ,  Wolfgang Hinz

    IPC分类号： G01N27/414

    CPC分类号： G01N27/4145 ,  C12Q1/6869 ,  G01N27/414 ,  Y10T436/143333

    摘要： A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. The well wall structure defines an upper surface and defines a wall surface extending between the upper surface and the lower surface. The system further includes a conductive layer disposed over the lower surface and the wall surface.

    摘要翻译： 一种系统包括传感器，其包括传感器垫和井壁结构，其限定了良好地操作地耦合到传感器垫的结构。 该井由设置在传感器垫上的下表面进一步限定。 井壁结构限定了上表面并且限定了在上表面和下表面之间延伸的壁表面。 该系统还包括设置在下表面和壁表面上的导电层。

    METHODS AND APPRATUS FOR PROTEIN AND PEPTIDE SEQUENCING
    5.
    发明公开
    METHODS AND APPRATUS FOR PROTEIN AND PEPTIDE SEQUENCING 审中-公开

    公开(公告)号：US20240200058A1

    公开(公告)日：2024-06-20

    申请号：US18529329

    申请日：2023-12-05

    申请人： Shifeng LI

    发明人： Shifeng LI

    IPC分类号： C12N15/10 ,  G01N33/53 ,  G01N33/68

    CPC分类号： C12N15/1065 ,  G01N33/5308 ,  G01N33/6824

    摘要： This disclosure is directed to a method for sequencing protein and peptide. The method specifies the steps of providing a polypeptide that is immobilized on solid surfaces and binding a N-terminal amino acid (NTAA) of the polypeptide with a modification molecule. Further, the method requires contacting the polypeptide with a NTAA binding molecule with a DNA coding tag, transferring an information from the DNA coding tag of the NTAA binding molecule to a universal primer and then forming an extended DNA tag on the solid surfaces, and cleaving the N-terminal amino acid on the polypeptide. Then, step (b) through step (e) are cyclically repeated. Finally, extended DNA strains are decoding on the solid surfaces.

    SCANNING PROBE EPITAXY
    6.
    发明申请
    SCANNING PROBE EPITAXY 审中-公开
    扫描探头外观

    公开(公告)号：US20100115672A1

    公开(公告)日：2010-05-06

    申请号：US12465615

    申请日：2009-05-13

    申请人： Chad A. Mirkin ,  Chang Liu ,  Yuhuang Wang ,  Adam B. Braunschweig ,  Xing Liao ,  Louise R. Giam ,  Byung Y. Lee ,  Shifeng Li

    发明人： Chad A. Mirkin ,  Chang Liu ,  Yuhuang Wang ,  Adam B. Braunschweig ,  Xing Liao ,  Louise R. Giam ,  Byung Y. Lee ,  Shifeng Li

    IPC分类号： G01Q60/24

    CPC分类号： G03F7/0002 ,  G01Q20/04 ,  G01Q40/00 ,  G01Q70/10 ,  G01Q70/16 ,  G01Q80/00

    摘要： A dual tip probe for scanning probe epitaxy and a method of forming the dual tip probe are disclosed. The dual tip probe includes first and second tips disposed on a cantilever arm. The first and second tips can be a reader tip and a synthesis tip, respectively. The first tip can remain in contact with a substrate during writing and provide in situ characterization of the substrate and or structures written, while the second tip can perform in non-contact mode to write and synthesis nanostructures. This feature can allow the dual tip probe to detect errors in a printed pattern using the first tip and correct the errors using the second tip.

    摘要翻译： 公开了一种用于扫描探针外延的双尖端探针和形成双尖端探针的方法。 双尖头探针包括设置在悬臂上的第一和第二尖端。 第一和第二提示可以分别是读取器尖端和合成尖端。 第一尖端可以在书写期间保持与基底接触，并且提供衬底和/或结构的原位表征，而第二尖端可以以非接触模式执行以写入和合成纳米结构。 该功能可以允许双尖端探针使用第一个尖端检测印刷图案中的错误，并使用第二个尖端校正错误。

    Chemical Coating of Microwell for Electrochemical Detection Device
    7.
    发明申请
    Chemical Coating of Microwell for Electrochemical Detection Device 审中-公开
    电化学检测装置的微孔化学涂层

    公开(公告)号：US20120045368A1

    公开(公告)日：2012-02-23

    申请号：US13212685

    申请日：2011-08-18

    申请人： Wolfgang HINZ ,  John MAURO ,  Shifeng LI ,  James BUSTILLO

    发明人： Wolfgang HINZ ,  John MAURO ,  Shifeng LI ,  James BUSTILLO

    IPC分类号： G01N30/00 ,  H01L21/02

    CPC分类号： G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

    摘要： The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

    摘要翻译： 所描述的实施例可以提供一种制造化学检测装置的方法。 该方法可以包括在CMOS器件上形成微孔。 微孔可以包括底表面和侧壁。 该方法可以进一步包括施加第一化学物质以选择性地连接到微孔的底表面，在微孔的侧壁上形成金属氧化物层，以及施加第二化学物质以选择性地连接到微孔的侧壁。 第二种化学物质可能与第一种化学物质不具有亲和力。