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    Epitope-captured antibody display
    3.
    发明授权
    Epitope-captured antibody display 失效
    表位捕获抗体显示

    公开(公告)号：US07741062B2

    公开(公告)日：2010-06-22

    申请号：US12420001

    申请日：2009-04-07

    申请人： William J. Dower ,  Steven E. Cwirla

    发明人： William J. Dower ,  Steven E. Cwirla

    IPC分类号： G01N33/53

    CPC分类号： G01N33/6842 ,  C07K16/28 ,  C07K16/2878 ,  C40B30/04 ,  G01N33/531 ,  G01N33/6845 ,  G01N33/6857 ,  G01N2035/00158 ,  G01N2600/00

    摘要： Reagents and methods for detecting target proteins in a sample are provided. The reagents include a replicable genetic package, a protein displayed on an exterior surface of the package that is expressed from a heterologous nucleic acid borne by the package, and one or more antibodies complexed with the expressed protein and which have an open binding site for a target protein. Thus, a segment of the nucleic acid encodes for an epitope that is shared by the expressed polypeptide and the target protein. The reagents can be utilized individually or as part of a library or an array to bind target proteins within protein samples to form one or more complexes. By determining the sequence of the segment of the heterologous nucleic acid of a package within a complex, one can identify the target protein since the segment encodes for an epitope that is shared by the expressed and target proteins.

    摘要翻译： 提供了用于检测样品中靶蛋白的试剂和方法。 试剂包括可复制的遗传包装，显示在由包装所携带的异源核酸表达的包装外表面上的蛋白质，以及与表达的蛋白质复合的一种或多种抗体，并且具有用于 靶蛋白。 因此，一段核酸编码由表达的多肽和靶蛋白共享的表位。 试剂可以单独使用或作为文库或阵列的一部分来结合蛋白质样品中的靶蛋白以形成一种或多种复合物。 通过确定复合物内的包装的异源核酸的片段的序列，可以鉴定靶蛋白，因为片段编码由表达的和靶蛋白共享的表位。

    Compounds displayed on icosahedral phage and methods of using same
    7.
    发明授权
    Compounds displayed on icosahedral phage and methods of using same 失效
    二十面体噬菌体上显示的化合物及其使用方法

    公开(公告)号：US07229757B2

    公开(公告)日：2007-06-12

    申请号：US10105029

    申请日：2002-03-21

    申请人： Ronald W. Barrett ,  William J. Dower ,  Mark Gallop ,  Thomas F. Woiwode ,  Steven E. Cwirla

    发明人： Ronald W. Barrett ,  William J. Dower ,  Mark Gallop ,  Thomas F. Woiwode ,  Steven E. Cwirla

    IPC分类号： C12Q1/68

    CPC分类号： C40B40/02 ,  C12N15/1037 ,  G01N33/6845

    摘要： Icosahedral phage and collections thereof that display various compounds are provided. In some instances, the icosahedral phage include nucleic acid tags that serve to record a characteristic of the compound or compounds that are attached to the phage. A number of different methods for using the icosahedral phage to screen a library of compounds for a desired biological activity are also provided, especially assays for compounds that are substrates for receptor-mediated transport processes such as endocytosis and transcytosis.

    摘要翻译： 提供显示各种化合物的二十面体噬菌体及其收集物。 在一些情况下，二十面体噬菌体包括用于记录附着于噬菌体的化合物或化合物的特征的核酸标签。 还提供了许多用于使用二十面体噬菌体筛选化合物文库以获得所需生物学活性的不同方法，特别是用于受体介导的转运过程例如内吞作用和转胞吞作用的底物的化合物的测定。