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公开(公告)号：US20150253284A1
公开(公告)日：2015-09-10
申请号：US14493729
申请日：2014-09-23
发明人： Arjun Sudarsan , Zhishan Hua
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L2200/0668 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/686 , G01N33/54326 , G01N2035/00425 , G01N2035/1058 , G01N2035/1062 , C12Q2549/101 , C12Q2527/15 , C12Q2527/107 , C12Q2561/113
摘要： The invention is directed to certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuator methods for detection of single nucleotide polymorphisms (SNPs) in a DNA sequence using digital microfluidics, including droplet actuator-based sample preparation and SNP analysis. In another embodiment, the invention provides droplet actuator devices and methods for providing integrated sample preparation and multiplexed detection of an infectious agent, such as HIV. In yet another embodiment, the invention provides droplet actuator devices and techniques for PCR amplification and detection of specific nucleic acid sequences using digital microfluidics, including droplet actuator-based sample preparation and target nucleic acid analysis. In yet another embodiment the invention provides methods for performing hot-start PCR on a droplet actuator. In yet another embodiment, the method of the invention combines PCR amplification with pyrosequencing to investigate specific sequences.
摘要翻译： 本发明涉及某些液滴致动分子技术。 在一个实施方案中，本发明提供用于使用数字微流体（包括基于液滴致动器的样品制备和SNP分析）检测DNA序列中的单核苷酸多态性（SNP）的液滴致动器方法。 在另一个实施方案中，本发明提供了液滴致动器装置和用于提供综合样品制备和多重检测感染因子如HIV的方法。 在另一个实施方案中，本发明提供了使用数字微流体（包括基于液滴致动器的样品制备和靶核酸分析）的PCR扩增和检测特异性核酸序列的液滴致动器装置和技术。 在另一个实施方案中，本发明提供了在液滴致动器上进行热启动PCR的方法。 在另一个实施方案中，本发明的方法将PCR扩增与焦磷酸测序结合以研究特异性序列。
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公开(公告)号：US09630180B2
公开(公告)日：2017-04-25
申请号：US14590470
申请日：2015-01-06
发明人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Y. Paik
CPC分类号： B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration may include one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
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公开(公告)号：US20160108432A1
公开(公告)日：2016-04-21
申请号：US14890451
申请日：2014-05-16
发明人： Srikoundinya Punnamaraju , Melissa A. Sandahl , John J. Harrington , Allen E. Eckhardt , Zhishan Hua , Michael G. Pollack
摘要： The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.
摘要翻译： 本发明提供了一种设计用于在液滴上对细胞进行电穿孔的液滴致动器。 本发明还提供了用于在液滴致动器上的液滴中对细胞进行电穿孔的方法和系统。
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公开(公告)号：US20140008222A1
公开(公告)日：2014-01-09
申请号：US14027960
申请日：2013-09-16
IPC分类号： B01L3/02
CPC分类号： G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要： A method of loading a droplet actuator is provided. In one embodiment, the method may include, providing: a droplet actuator loaded with a filler fluid; a reservoir comprising a droplet fluid; and a fluid path extending from the reservoir into the droplet actuator; and forcing filler fluid from one locus in the droplet actuator to another locus in the droplet actuator; or out of the droplet actuator; thereby causing droplet fluid to flow through the fluid path and into the droplet actuator.
摘要翻译： 提供了一种装载液滴致动器的方法。 在一个实施例中，该方法可以包括：提供：负载有填充流体的液滴致动器; 包括液滴流体的储存器; 以及从储存器延伸到液滴致动器中的流体路径; 并且迫使填充液从液滴致动器中的一个轨迹到液滴致动器中的另一个轨迹; 或离开液滴致动器; 从而使液滴流体流过流体路径并进入液滴致动器。
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公开(公告)号：US09952177B2
公开(公告)日：2018-04-24
申请号：US14795304
申请日：2015-07-09
发明人： Michael G. Pollack , Vijay Srinivasan , Zhishan Hua , Hon Lung Chu , Michael Hauser , Allen Eckhardt
IPC分类号： G01N27/447 , G01N27/453 , B01L3/00 , B01L7/00
CPC分类号： G01N27/44743 , B01L3/502715 , B01L3/502792 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2300/088 , B01L2300/0887 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/043 , B01L2400/0457 , B01L2400/046 , G01N27/44791
摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.
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公开(公告)号：US09511369B2
公开(公告)日：2016-12-06
申请号：US14248884
申请日：2014-04-09
IPC分类号： B01L3/00
CPC分类号： B01L3/502792 , B01L2200/12 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0421 , B01L2400/0427 , Y10T29/49826
摘要： The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.
摘要翻译： 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。
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7.发明申请公开(公告)号：US20150323496A1
公开(公告)日：2015-11-12
申请号：US14795304
申请日：2015-07-09
发明人： Michael G. Pollack , Vijay Srinivasan , Zhishan Hua , Hon Lung Chu , Michael Hauser , Allen Eckhardt
IPC分类号： G01N27/447 , B01L3/00
CPC分类号： G01N27/44743 , B01L3/502715 , B01L3/502792 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2300/088 , B01L2300/0887 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/043 , B01L2400/0457 , B01L2400/046 , G01N27/44791
摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.
摘要翻译： 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。
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公开(公告)号：US20140216932A1
公开(公告)日：2014-08-07
申请号：US14248884
申请日：2014-04-09
IPC分类号： B01L3/00
CPC分类号： B01L3/502792 , B01L2200/12 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0421 , B01L2400/0427 , Y10T29/49826
摘要： The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.
摘要翻译： 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。
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公开(公告)号：US20140014517A1
公开(公告)日：2014-01-16
申请号：US14027941
申请日：2013-09-16
IPC分类号： G01N27/447
CPC分类号： G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要： Droplet actuator for conducting droplet operations, such as droplet transport and droplet dispensing, is provided. In one embodiment, the droplet actuator may include an electrode that is rotationally but not reflectively symmetrical.
摘要翻译： 提供用于进行液滴操作的液滴致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括旋转但不反射对称的电极。
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