公开(公告)号：US20140329307A1

公开(公告)日：2014-11-06

申请号：US14290057

申请日：2014-05-29

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Alexander Shenderov ,  Michael G. Pollack

IPC: B01L7/00

CPC classification number: B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

Abstract translation: 描述了需要多个反应温度的用于进行化学或生化反应的方法和装置。 所述方法包括在微流体装置上移动一个或多个反应液滴或反应体积通过具有不同温度的各种反应区。 这些装置包括微流体装置，其包括能够通过各种反应区移动反应液滴或反应体积的适当致动器。

公开(公告)号：US09861986B2

公开(公告)日：2018-01-09

申请号：US14506265

申请日：2014-10-03

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Prasanna Thwar ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Alexander Shenderov ,  Dwayne J. Allen

IPC: B01L3/00 ,  G01N27/447 ,  B01L7/00 ,  G01N35/10 ,  G01N35/00

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0683 ,  G01N27/44704 ,  G01N35/0098 ,  G01N2035/1046 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.

公开(公告)号：US09511369B2

公开(公告)日：2016-12-06

申请号：US14248884

申请日：2014-04-09

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Alexander Shenderov ,  Zhishan Hua ,  Arjun Sudarsan

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0427 ,  Y10T29/49826

Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.

Abstract translation: 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。

公开(公告)号：US20150027889A1

公开(公告)日：2015-01-29

申请号：US14506265

申请日：2014-10-03

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Prasanna Thwar ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Alexander Shenderov ,  Dwayne J. Allen

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0683 ,  G01N27/44704 ,  G01N35/0098 ,  G01N2035/1046 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.

Abstract translation: 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒，以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。

公开(公告)号：US20140216932A1

公开(公告)日：2014-08-07

申请号：US14248884

申请日：2014-04-09

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Alexander Shenderov ,  Zhishan Hua ,  Arjun Sudarsan

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0427 ,  Y10T29/49826

Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.

Abstract translation: 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。

公开(公告)号：US09452433B2

公开(公告)日：2016-09-27

申请号：US14290057

申请日：2014-05-29

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Alexander Shenderov ,  Michael G. Pollack

IPC: B01L7/00 ,  B01L3/00

CPC classification number: B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

公开(公告)号：US20140251807A1

公开(公告)日：2014-09-11

申请号：US14281084

申请日：2014-05-19

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Alexander Shenderov

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2005/0017 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0427 ,  F04B17/00 ,  F04B19/006 ,  F16K99/0001 ,  F16K99/0017 ,  G02B26/005

Abstract: A series of microactuators for manipulating small quantities of liquids, and methods of using these for manipulating liquids, are disclosed. The microactuators are based on the phenomenon of electrowetting and contain no moving parts. The force acting on the liquid is a potential-dependent gradient of adhesion energy between the liquid and a solid insulating surface.

Abstract translation: 公开了一系列用于操作少量液体的微致动器以及使用它们来操纵液体的方法。 微致动器基于电润湿现象，不含运动部件。 作用在液体上的力是液体和固体绝缘表面之间的粘附能的电势梯度。