公开(公告)号：US20150376573A1

公开(公告)日：2015-12-31

申请号：US14850679

申请日：2015-09-10

申请人： ANTEROGEN CO., LTD.

发明人： Sung-Koo LEE ,  Mi-Hyung KIM

IPC分类号： C12N5/0775 ,  A61K35/28

CPC分类号： C12N5/0667 ,  A61K35/12 ,  A61K35/28 ,  C12N2501/115

摘要： The present invention relates to a method for producing clinically effective quantities of human adipose tissue-derived stromal cells for treating fistulas and a composition made with the same. The method of the present invention can efficiently produce clinically effective number of adipose tissue-derived stromal cells within a short period by improving conventional standard culturing methods. The adipose stem cells composition containing the adipose tissue-derived stromal cells obtained by the method of the present invention exhibit superior multipotency and immunomodulatory activity over those of a cell composition produced by conventional methods, and thus is more suitable for treating fistulas. The cell composition of the present invention has excellent clinical usages especially since immune response is suppressed in allogenic adipose-derived stem cells.

摘要翻译： 本发明涉及一种临床有效量的用于治疗瘘的人体脂肪组织来源的基质细胞的方法及其制备的组合物。 本发明的方法可以通过改进常规的标准培养方法在短时间内有效地产生临床有效数量的脂肪组织来源的基质细胞。 含有通过本发明方法获得的脂肪组织来源的基质细胞的脂肪干细胞组合物与通过常规方法制备的细胞组合物相比具有优异的多能性和免疫调节活性，因此更适合于治疗瘘。 本发明的细胞组合物具有优异的临床用途，特别是因为在同种异体脂肪干细胞中免疫应答被抑制。

公开(公告)号：US20230405180A1

公开(公告)日：2023-12-21

申请号：US18158215

申请日：2023-01-23

申请人： ANTEROGEN CO., LTD.

发明人： Sung-Koo LEE ,  Mihyung KIM ,  Inok KIM

IPC分类号： A61L26/00 ,  A61L15/40 ,  A61L27/52 ,  A61L27/38 ,  A61L27/60 ,  A61K35/28 ,  C12N5/0775

CPC分类号： A61L26/008 ,  C12N2533/56 ,  A61L27/52 ,  A61L27/3834 ,  A61L27/60 ,  A61K35/28 ,  A61L26/0052 ,  A61L26/0057 ,  C12N5/0667 ,  C12N2533/54 ,  C12N2533/78 ,  C12N2533/80 ,  A61L2300/412 ,  A61L2300/64 ,  C12N2533/30 ,  A61L15/40

摘要： The present invention relates to a composition comprising mesenchymal stem cell-hydrogel-biodegradable support or mesenchymal stem cell-hydrogel-undegradable support, a sheet comprising the composition and a method for the preparation thereof. By using the sheet comprising the adipose-derived mesenchymal stem cell-hydrogel biodegradable or undegradable support, stem cells of high activity may be applied directly to the wound without isolation process using protease. The sheet has extracellular matrices such as collagen, laminin, fibronectin and elastin secreted by stem cells in the culture stage and included completely in the hydrogel, and therefore it has superior skin regeneration and wound healing effects compared with conventional pharmaceutical preparations and shortens therapeutic period.

公开(公告)号：US20220175666A1

公开(公告)日：2022-06-09

申请号：US17437306

申请日：2020-03-20

申请人： ANTEROGEN CO., LTD.

发明人： Sung-Koo LEE ,  Mi-Hyung KIM

IPC分类号： A61K9/00 ,  A61K35/28 ,  A61K9/16 ,  A61P19/02 ,  A61P29/00 ,  C12N11/04

摘要： The present invention relates to a composition containing injectable mesenchymal stem cell-hydrogel and a method of preparing the same. Specifically, in a mesenchymal stem cell-hydrogel composition for injection prepared by a method of the present invention, since stem cells are attached to scaffolds in hydrogel beads, the stem cells are not easily lost or killed after the injection, and thus there is an advantage that an engraftment rate increases since the paracrine effect of the stem cells is continuously exhibited, and the stem cells are gradually released as hydrogel is degraded. In addition, the present invention has an advantage that healthy cells can be used without damages in cell membranes since injection formulation can be prepared without a treatment with proteolytic enzymes, and also a cryopreservation solution is easily removed from the mesenchymal stem cell-hydrogel beads even after freezing and thawing.

公开(公告)号：US20220378982A1

公开(公告)日：2022-12-01

申请号：US17819220

申请日：2022-08-11

申请人： ANTEROGEN CO., LTD.

发明人： Sung-Koo LEE ,  Mihyung KIM

IPC分类号： A61L27/38 ,  A61K9/70 ,  A61K9/00 ,  A61P17/00 ,  A61K38/39 ,  A61K47/30 ,  A61L27/52 ,  A61L27/54 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0775

摘要： Provided are a composition and a sheet, including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support and a preparing method thereof. More specifically, in the sheet including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support according to the present invention, the high-active mesenchymal stem cells may be applied to a wounded part of a patient with epidermolysis bullosa as it is without isolation using proteases, and in the culturing, an extracellular matrix such as collagen, laminin, fibronectin, and elastin secreted from the mesenchymal stem cells is wholly present on the hydrogel to have an advantageous effect that skin reproduction and re-epithelization abilities are significantly excellent as compared with conventional dressing agents used for epidermolysis bullosa.

公开(公告)号：US20180117217A1

公开(公告)日：2018-05-03

申请号：US15803132

申请日：2017-11-03

申请人： ANTEROGEN CO., LTD

发明人： Sung-Koo LEE ,  Mihyung KIM

IPC分类号： A61L27/38 ,  C12N5/0775 ,  A61K38/39 ,  A61K47/30 ,  A61L27/52 ,  C12N5/071 ,  C12N5/00 ,  A61P17/00 ,  A61L27/54

CPC分类号： A61L27/3813 ,  A61K9/0014 ,  A61K9/70 ,  A61K38/39 ,  A61K47/30 ,  A61L27/3834 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/20 ,  A61L2300/64 ,  A61P17/00 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0625 ,  C12N5/0665

摘要： Provided are a composition and a sheet, including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support and a preparing method thereof. More specifically, in the sheet including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support according to the present invention, the high-active mesenchymal stem cells may be applied to a wounded part of a patient with epidermolysis bullosa as it is without isolation using proteases, and in the culturing, an extracellular matrix such as collagen, laminin, fibronectin, and elastin secreted from the mesenchymal stem cells is wholly present on the hydrogel to have an advantageous effect that skin reproduction and re-epithelization abilities are significantly excellent as compared with conventional dressing agents used for epidermolysis bullosa.

公开(公告)号：US20170304366A1

公开(公告)日：2017-10-26

申请号：US15513247

申请日：2014-11-03

申请人： ANTEROGEN CO., LTD.

发明人： Sung-Koo LEE ,  Mihyung KIM ,  Jung In YOON

IPC分类号： A61K35/28 ,  A61K9/06 ,  C12N5/0775 ,  A61K47/42 ,  C08J3/075

CPC分类号： A61K35/28 ,  A61K9/06 ,  A61K35/12 ,  A61K47/42 ,  C08J3/075 ,  C08J2389/00 ,  C12N5/0667 ,  C12N2533/56 ,  C12N2539/00

摘要： The present invention relates to a stable and readily usable administration type including a composition including mesenchymal stem cells cultured in hydrogel, more specifically, a adipose tissue-derived mesenchymal stem cell-hydrogel composition and a method of preparing the same. More specifically, the present invention relates to a composition including mesenchymal stem cells cultured in hydrogel, washed, and filled into a syringe, wherein the composition of the present invention may be readily administered without modification and may not need an enzyme treatment in a final process of preparing transplanted cells, almost no cell is lost because the composition is washed as hydrogel without an washing process using centrifugation or other methods, and the therapeutic effect is shown immediately after administration to an individual because bioactive substances are patched in pores of the hydrogel.

公开(公告)号：US20160051722A1

公开(公告)日：2016-02-25

申请号：US14779065

申请日：2014-07-07

申请人： ANTEROGEN CO., LTD.

发明人： Sung-Koo LEE ,  Mihyung KIM ,  Inok KIM

IPC分类号： A61L26/00 ,  C12N5/0775

CPC分类号： A61L26/008 ,  A61K35/28 ,  A61L15/40 ,  A61L26/0052 ,  A61L26/0057 ,  A61L27/3834 ,  A61L27/52 ,  A61L27/60 ,  A61L2300/412 ,  A61L2300/64 ,  C12N5/0667 ,  C12N2533/30 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2533/78 ,  C12N2533/80

摘要： The present invention relates to a composition comprising mesenchymal stem cell-hydrogel-biodegradable support or mesenchymal stem cell-hydrogel-undegradable support, a sheet comprising the composition and a method for the preparation thereof. By using the sheet comprising the adipose-derived mesenchymal stem cell-hydrogel biodegradable or undegradable support, stem cells of high activity may be applied directly to the wound without isolation process using protease. The sheet has extracellular matrices such as collagen, laminin, fibronectin and elastin secreted by stem cells in the culture stage and included completely in the hydrogel, and therefore it has superior skin regeneration and wound healing effects compared with conventional pharmaceutical preparations and shortens therapeutic period.

摘要翻译： 本发明涉及包含间充质干细胞 - 水凝胶可生物降解支持物或间充质干细胞 - 水凝胶 - 不可降解支持物的组合物，包含该组合物的片剂及其制备方法。 通过使用包含脂肪来源的间充质干细胞 - 水凝胶可生物降解或不可降解支持物的片材，可以使用蛋白酶将高活性的干细胞直接施用于伤口而不进行分离。 该片材在培养阶段具有细胞外基质如干细胞分泌的胶原蛋白，层粘连蛋白，纤连蛋白和弹性蛋白，并完全包含在水凝胶中，因此与常规药物制剂相比具有优异的皮肤再生和伤口愈合效果，缩短了治疗期。