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公开(公告)号：US07425437B2
公开(公告)日：2008-09-16
申请号：US11110517
申请日：2005-04-20
CPC分类号： A61K39/12 , A61K2039/5252 , A61K2039/552 , A61K2039/55505 , A61K2039/55566 , C12N7/00 , C12N2770/24134 , C12N2770/24163
摘要： The present invention relates to novel vaccines containing (whole-inactivated) West Nile Viruses and/or West Nile viral proteins derived therefrom, produced on human cells, wherein the human cells comprise a sequence encoding at least one early region-1 (E1) gene product of an adenovirus. The cells are preferably cultured in suspension to very high densities and under serum-free conditions. Herein, it is disclosed that use of such cells results in high titers of West Nile Virus produced.
摘要翻译： 本发明涉及在人类细胞上产生的含有（全失活的）西尼罗病毒和/或由其衍生的西尼罗病毒蛋白质的新型疫苗，其中人细胞包含编码至少一个早期区域-1（E1）基因的序列 腺病毒的产物。 细胞优选在悬浮液中以非常高的密度和无血清条件培养。 在本文中，公开了使用这种细胞导致西尼罗病毒产生的高滴度。
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公开(公告)号：US20090017068A1
公开(公告)日：2009-01-15
申请号：US12220971
申请日：2008-07-29
CPC分类号： A61K39/12 , A61K2039/5252 , A61K2039/552 , A61K2039/55505 , A61K2039/55566 , C12N7/00 , C12N2770/24134 , C12N2770/24163
摘要： Described are vaccines containing (whole-inactivated) West Nile Viruses and/or West Nile viral proteins derived therefrom, produced on human cells, wherein the human cells comprise a sequence encoding at least one early region-1 (E1) gene product of an adenovirus. The cells are preferably cultured in suspension to very high densities and under serum-free conditions. Herein, it is disclosed that use of such cells results in high titers of West Nile Virus produced.
摘要翻译： 描述了在人细胞上产生的含有（全失活的）西尼罗病毒和/或由其衍生的西尼罗病毒蛋白质的疫苗，其中人细胞包含编码腺病毒的至少一个早期区域-1（E1）基因产物的序列 。 细胞优选在悬浮液中以非常高的密度和无血清条件培养。 在本文中，公开了使用这种细胞导致西尼罗病毒产生的高滴度。
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公开(公告)号：US20100172917A1
公开(公告)日：2010-07-08
申请号：US12590973
申请日：2009-11-16
IPC分类号： A61K39/42 , C07K16/08 , C07H21/00 , A61P37/02 , A61P11/00 , C12N15/63 , C12N5/07 , C12P21/00 , C40B30/04 , C40B40/10 , C12Q1/70
CPC分类号： G01N33/56983 , A61K2039/505 , C07K14/005 , C07K16/10 , C07K2317/21 , C07K2317/622 , C07K2317/76 , C12N7/00 , C12N2770/20022 , C12N2770/20061 , G01N2333/165
摘要： Described are binding molecules that specifically bind to SARS-CoV, nucleic acid molecules encoding the binding molecules, compositions comprising the binding molecules, and methods of identifying or producing the binding molecules. The binding molecules are capable of specifically binding to SARS-CoV, and can be used in the diagnosis, prophylaxis, and/or treatment of a condition resulting from SAR.
摘要翻译： 描述了特异性结合SARS-CoV的结合分子，编码结合分子的核酸分子，包含结合分子的组合物，以及鉴定或产生结合分子的方法。 结合分子能够特异性结合SARS-CoV，可用于诊断，预防和/或治疗由SAR产生的病症。
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公开(公告)号：US07696330B2
公开(公告)日：2010-04-13
申请号：US11337300
申请日：2006-01-20
IPC分类号： C07K16/00 , C12Q1/00 , G01N33/563
CPC分类号： G01N33/56983 , A61K2039/505 , C07K14/005 , C07K16/10 , C07K2317/21 , C07K2317/622 , C07K2317/76 , C12N7/00 , C12N2770/20022 , C12N2770/20061 , G01N2333/165
摘要： The present invention provides binding molecules that specifically bind to SARS-CoV, nucleic acid molecules encoding the binding molecules, compositions comprising the binding molecules and methods of identifying or producing the binding molecules. The binding molecules are capable of specifically binding to SARS-CoV and can be used in the diagnosis, prophylaxis and/or treatment of a condition resulting from SARS-CoV.
摘要翻译： 本发明提供了特异性结合SARS-CoV的结合分子，编码结合分子的核酸分子，包含结合分子的组合物以及鉴定或产生结合分子的方法。 结合分子能够特异性结合SARS-CoV，可用于诊断，预防和/或治疗由SARS-CoV引起的病症。
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公开(公告)号：US20080131461A1
公开(公告)日：2008-06-05
申请号：US11665393
申请日：2005-10-13
申请人： Maria Grazia Pau , Jaap Goudsmit , Joseph D. Cohen , Patrice Dubois , V.Ann Stewart , Donald Heppner
发明人： Maria Grazia Pau , Jaap Goudsmit , Joseph D. Cohen , Patrice Dubois , V.Ann Stewart , Donald Heppner
CPC分类号： A61K39/015 , A61K2039/5256 , A61K2039/55572 , A61K2039/55577 , A61K2039/6075 , Y02A50/412
摘要： The invention relates to novel vaccine regimens in which specific prime/boost regimens are applied using low-neutralized recombinant adenoviral vectors harboring nucleic acids encoding antigens from Plasmodium falciparum and purified recombinant protein vaccines such as RTS,S, in the context of appropriate adjuvants.
摘要翻译： 本发明涉及新颖的疫苗方案，其中使用含有编码来自恶性疟原虫的抗原的核酸的低中和重组腺病毒载体和在合适佐剂的情况下使用纯化的重组蛋白疫苗如RTS，S应用特异性初始/加强方案。
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6.发明申请公开(公告)号：US20060223056A1
公开(公告)日：2006-10-05
申请号：US11372577
申请日：2006-03-10
CPC分类号： C12Q1/703 , C12Q1/6848 , Y10S435/81
摘要： The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
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公开(公告)号：US20060121580A1
公开(公告)日：2006-06-08
申请号：US11337300
申请日：2006-01-20
CPC分类号： G01N33/56983 , A61K2039/505 , C07K14/005 , C07K16/10 , C07K2317/21 , C07K2317/622 , C07K2317/76 , C12N7/00 , C12N2770/20022 , C12N2770/20061 , G01N2333/165
摘要： The present invention provides binding molecules that specifically bind to SARS-CoV, nucleic acid molecules encoding the binding molecules, compositions comprising the binding molecules and methods of identifying or producing the binding molecules. The binding molecules are capable of specifically binding to SARS-CoV and can be used in the diagnosis, prophylaxis and/or treatment of a condition resulting from SAR
摘要翻译： 本发明提供了特异性结合SARS-CoV的结合分子，编码结合分子的核酸分子，包含结合分子的组合物以及鉴定或产生结合分子的方法。 结合分子能够特异性结合SARS-CoV，可用于诊断，预防和/或治疗由SAR产生的病症
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8.发明申请ADENOVIRUS SEROTYPE 26 AND SEROTYPE 35 FILOVIRUS VACCINES 有权ADENOVIRUS SEROTYPE 26和SEROTYPE 35 FILOVIRUS VACCINES公开(公告)号：US20140017278A1
公开(公告)日：2014-01-16
申请号：US13994532
申请日：2011-12-14
申请人： Nancy J. Sullivan , Gary J. Nabel , Clement Asiedu , Cheng Cheng , Maria Grazia Pau , Jaap Goudsmit
发明人： Nancy J. Sullivan , Gary J. Nabel , Clement Asiedu , Cheng Cheng , Maria Grazia Pau , Jaap Goudsmit
IPC分类号： C07K14/005
CPC分类号： C07K14/005 , A61K39/12 , A61K2039/5254 , A61K2039/5256 , A61K2039/70 , C12N2710/10034 , C12N2710/10043 , C12N2760/14034 , C12N2760/14134
摘要： Provided are recombinant adenovirus vectors (serotype 26 and serotype 35) encoding filovirus antigens. The adenovirus vectors can be used to induce protective immune responses against filovirus infection.
摘要翻译： 提供了编码病毒抗原的重组腺病毒载体（血清型26和血清型35）。 腺病毒载体可用于诱导针对病毒感染的保护性免疫应答。
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公开(公告)号：US20120014994A1
公开(公告)日：2012-01-19
申请号：US13199273
申请日：2011-08-23
申请人： Maria-Grazia Pau , Jaap Goudsmit , Joseph D. Cohen , Patrice M. Dubois , V. Ann Stewart , Donald Gray Heppner, JR.
发明人： Maria-Grazia Pau , Jaap Goudsmit , Joseph D. Cohen , Patrice M. Dubois , V. Ann Stewart , Donald Gray Heppner, JR.
IPC分类号： A61K39/015 , A61P33/06 , A61K39/002
CPC分类号： A61K39/015 , A61K2039/5256 , A61K2039/55572 , A61K2039/55577 , A61K2039/6075 , Y02A50/412
摘要： Described are vaccine regimens in which specific prime/boost regimens are applied using low-neutralized recombinant adenoviral vectors harboring nucleic acids encoding antigens from Plasmodium falciparum and purified recombinant protein vaccines such as RTS,S, in the context of appropriate adjuvants.
摘要翻译： 描述了在适当佐剂的情况下，使用含有编码来自恶性疟原虫的抗原的核酸的低中和重组腺病毒载体和诸如RTS，S的纯化的重组蛋白疫苗来应用特异性初始/加强方案的疫苗方案。
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公开(公告)号：US07238530B2
公开(公告)日：2007-07-03
申请号：US09760085
申请日：2001-01-12
申请人： Jaap Goudsmit , Cornelis Johannes Andreas Sol , Marcellinus Gualbertus Hubertus Maria Beld , Willem René Boom
发明人： Jaap Goudsmit , Cornelis Johannes Andreas Sol , Marcellinus Gualbertus Hubertus Maria Beld , Willem René Boom
CPC分类号： C12N15/1006 , C12N15/10 , C12Q1/6806 , C12Q1/6855 , C12Q2527/137 , C12Q2527/125
摘要： The invention relates to methods for easily separating single stranded nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at lease one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such a chelating agents, pH and the like, it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficent separations, the process may be repeated. Following the separation of the two kinds of nucleic acid, either kind may be amplified. Methods of amplification are provided which do not need sequence data of the material to be amplified. In these methods a primer will be provided with an amplification motif and a random hybridization motif.
摘要翻译： 本发明涉及在含有两者的样品中容易地从双链核酸材料中分离单链核酸材料的方法。 通过正确选择一种离液剂，优选胍盐，以选定的浓度和其它合适的条件如螯合剂，pH等，可以将双链材料结合到固相如二氧化硅颗粒 而单链材料在这种情况下不会结合。 通过从样品中分离二氧化硅颗粒，除去双链核酸材料。 它可以容易地从二氧化硅颗粒中洗脱出来。 在第二步中，单链材料可以通过选择不同的条件集合而与固相结合。 颗粒可以再次与样品分离，单链材料现在可以被洗脱。 为了非常有效的分离，可以重复该过程。 在分离两种核酸后，可以扩增任一种。 提供了不需要放大材料的序列数据的扩增方法。 在这些方法中，引物将具有扩增基序和随机杂交基序。
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