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1.发明授权Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs 失效用于在种植和培养三维组织构建体时模拟体内条件的装置和方法
公开(公告)号：US6121042A
公开(公告)日：2000-09-19
申请号：US1609
申请日：1997-12-31
申请人： Alvin Peterson , Lee K. Landeen , John Bennett , Jason Gee , Scott Chesla , Joan Zeltinger , James H. Flatt , Mark A. Applegate , Noushin Dunkelman , Stephen V. Kemmerrer
发明人： Alvin Peterson , Lee K. Landeen , John Bennett , Jason Gee , Scott Chesla , Joan Zeltinger , James H. Flatt , Mark A. Applegate , Noushin Dunkelman , Stephen V. Kemmerrer
CPC分类号： C12M35/04 , C12M41/00 , A61F2/30756
摘要： An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing three-dimensional tissue constructs is disclosed. The apparatus includes a fluid reservoir, a pump, at least one treatment chamber, and a means for controlling media flow characteristics around a tissue construct disposed within the treatment chamber, and for controlling movement of the construct itself, so as to simulate a variety of physiologic conditions. One exemplary embodiment of the invention includes a means for applying an axial stress to the construct. Applying an axial stress to the construct during seeding and culturing results in a tissue-engineered construct with cells and their fibers oriented in a manner which is more likely to possess long term dimensional stability and the patency of native vessels with normal physiologic function.
摘要翻译： 公开了用于灭菌，播种，培养，储存，运送和测试三维组织结构的装置和方法。 该装置包括流体储存器，泵，至少一个处理室，以及用于控制设置在处理室内的组织结构周围的介质流动特性的装置，并且用于控制构造体本身的移动，以便模拟各种 生理条件。 本发明的一个示例性实施例包括用于向结构施加轴向应力的装置。 在播种和培养过程中对构建体施加轴向应力导致组织工程构建体中细胞及其纤维以更可能具有长期尺寸稳定性和具有正常生理功能的天然血管通畅的方式取向。
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2.发明授权Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing tissue, synthetic, or mechanical heart valves orvalve segments 失效组织，合成或机械心脏瓣膜或瓣膜段的消毒，播种，培养，储存，运输和测试的装置和方法
公开(公告)号：US5846828A
公开(公告)日：1998-12-08
申请号：US478309
申请日：1995-06-07
申请人： Alvin Peterson , Lee K. Landeen , John Bennett , Jason Gee , Scott Chesla , Joan Zeltinger
发明人： Alvin Peterson , Lee K. Landeen , John Bennett , Jason Gee , Scott Chesla , Joan Zeltinger
CPC分类号： C12M29/12 , A61F2/062 , A61F2/2415 , Y10S623/922
摘要： An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing heart valves is disclosed. Specifically, the present invention relates to an apparatus and method for dynamically seeding and culturing heart valves with human cells, resulting in a heart valve populated with viable human cells. In a preferred embodiment, the apparatus includes a fluid reservoir, a pneumatic pressure chamber, a pressure source for providing a varying pressure to the pressure chamber, and a heart valve holder. In an alternative exemplary embodiment, the apparatus includes a fluid reservoir, a pump, a venturi tube, a heart valve chamber, a heart valve holder, and a valve connected to a timer. In each embodiment, the components are combined in a fluid circuit to create a variable cycling and pulsatile fluid flow for seeding and culturing which can closely resemble the physiological conditions found in the human heart.
摘要翻译： 公开了一种用于灭菌，播种，培养，储存，运输和测试心脏瓣膜的装置和方法。 具体而言，本发明涉及用人体细胞动态播种和培养心脏瓣膜的装置和方法，导致心脏瓣膜内含有活细胞。 在优选实施例中，该装置包括流体储存器，气动压力室，用于向压力室提供变化的压力的压力源和心脏瓣膜保持器。 在替代的示例性实施例中，该装置包括流体储存器，泵，文氏管，心脏瓣膜腔，心脏瓣膜保持器和连接到计时器的阀。 在每个实施例中，组件被组合在流体回路中以产生用于播种和培养的可变循环和脉动流体流，其可以非常类似于在人心脏中发现的生理条件。
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公开(公告)号：US20120230940A1
公开(公告)日：2012-09-13
申请号：US13417017
申请日：2012-03-09
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
IPC分类号： A61K35/12 , A61K38/18 , A61K31/506 , C12N5/073
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
摘要翻译： 描述了包含调节细胞培养基组合物和使用方法的新产品。 本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。 一旦本发明的细胞培养基被调节，它可以在任何状态下使用。 条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。 另外，介质配制有药学上可接受的载体作为用于内部施用的载体，直接施用于食品或产品，或用药膏或软膏配制用于局部应用。 此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。
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公开(公告)号：US20090123503A1
公开(公告)日：2009-05-14
申请号：US12349451
申请日：2009-01-06
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
摘要翻译： 描述了包含调节细胞培养基组合物和使用方法的新产品。 本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。 介质可以由基质细胞，实质细胞，间充质干细胞，肝储备细胞，神经干细胞，胰干细胞和/或胚胎干细胞调节。 另外，细胞可以被遗传修饰。 优选三维组织构建体。 一旦本发明的细胞培养基被调节，它可以在任何状态下使用。 条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。 另外，介质配制有药学上可接受的载体作为内部给药载体，直接施用于用药膏或软膏配制用于局部应用的食品或产品，或者例如制成或添加到手术胶中 通过侵入性手术加快缝合线愈合。 此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。
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公开(公告)号：US08476231B2
公开(公告)日：2013-07-02
申请号：US13417017
申请日：2012-03-09
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
摘要翻译： 描述了包含调节细胞培养基组合物和使用方法的新产品。 本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。 一旦本发明的细胞培养基被调节，它可以在任何状态下使用。 条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。 另外，介质配制有药学上可接受的载体作为用于内部施用的载体，直接施用于食品或产品，或用药膏或软膏配制用于局部应用。 此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。
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公开(公告)号：US08361485B2
公开(公告)日：2013-01-29
申请号：US11538380
申请日：2006-10-03
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
摘要翻译： 描述了包含调节细胞培养基组合物和使用方法的新产品。 本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。 介质可以由基质细胞，实质细胞，间充质干细胞，肝储备细胞，神经干细胞，胰干细胞和/或胚胎干细胞调节。 另外，细胞可以被遗传修饰。 优选三维组织构建体。 一旦本发明的细胞培养基被调节，它可以在任何状态下使用。 条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。 另外，介质配制有药学上可接受的载体作为内部给药载体，直接施用于用药膏或软膏配制用于局部应用的食品或产品，或者例如制成或添加到手术胶中 通过侵入性手术加快缝合线愈合。 此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。
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公开(公告)号：US07118746B1
公开(公告)日：2006-10-10
申请号：US09979813
申请日：2000-05-12
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
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公开(公告)号：US08138147B2
公开(公告)日：2012-03-20
申请号：US12349451
申请日：2009-01-06
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
摘要翻译： 描述了包含调节细胞培养基组合物和使用方法的新产品。 本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。 介质可以由基质细胞，实质细胞，间充质干细胞，肝储备细胞，神经干细胞，胰干细胞和/或胚胎干细胞调节。 另外，细胞可以被遗传修饰。 优选三维组织构建体。 一旦本发明的细胞培养基被调节，它可以在任何状态下使用。 条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。 另外，介质配制有药学上可接受的载体作为内部给药载体，直接施用于用药膏或软膏配制用于局部应用的食品或产品，或者例如制成或添加到手术胶中 通过侵入性手术加快缝合线愈合。 此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。
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9.发明授权公开(公告)号：US08128924B2
公开(公告)日：2012-03-06
申请号：US12420716
申请日：2009-04-08
IPC分类号： C12N5/00
CPC分类号： A61L27/3886 , A61L27/3804 , A61L27/3839 , A61L27/507
摘要： The present invention relates to a method for promoting blood vessel formation in tissues and organs. In particular, the method relates to implantation or attachment of an engineered three-dimensional stromal tissue to promote endothelialization and angiogenesis in the heart and related tissues. The three-dimensional stromal tissue of the present invention may be used in a variety of applications including, but not limited to, promoting repair of and regeneration of damaged cardiac muscle, promoting vascularization and healing during cardiac surgery, promoting blood vessel formation at anastomosis sites, and promoting vascularization and repair of damaged skeletal muscle, smooth muscle or connective tissue.
摘要翻译： 本发明涉及促进组织器官中血管形成的方法。 特别地，该方法涉及植入或附着工程化的三维基质组织以促进心脏和相关组织中的内皮细胞化和血管发生。 本发明的三维基质组织可用于多种应用，包括但不限于促进损伤的心肌的修复和再生，促进心脏手术期间的血管化和愈合，促进吻合部位的血管形成 并促进损伤的骨骼肌，平滑肌或结缔组织的血管化和修复。
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公开(公告)号：US20110213456A1
公开(公告)日：2011-09-01
申请号：US13101391
申请日：2011-05-05
IPC分类号： A61F2/82 , C08F220/18 , C08F8/14
CPC分类号： A61L31/18 , A61F2/92 , A61K49/12 , A61K49/126 , A61K49/128 , A61L31/06 , C08F220/00 , C08G63/00 , C08G63/08
摘要： Side-chain crystallizable (SCC) polymers are useful in various medical applications. In certain applications, heavy atom containing side-chain crystallizable polymers (HACSCCP's) are particularly useful. An example of a HACSCCP is a polymer that comprises a main chain, a plurality of crystallizable side chains, and a plurality of heavy atoms attached to the polymer. In certain configurations, the heavy atoms are present in an amount that is effective to render the polymer radiopaque. A polymeric material that includes an HACSCCP may be fabricated into a medical device useful for at least partially occluding a body cavity. For example, such a medical device may be an embolotherapy product. A polymeric material that includes a SCC polymer may also be fabricated into other medical devices, such as stents.
摘要翻译： 侧链可结晶（SCC）聚合物可用于各种医疗应用。 在某些应用中，含重链原子的侧链可结晶聚合物（HACSCCP）特别有用。 HACSCCP的实例是包含主链，多个可结晶侧链和连接到聚合物上的多个重原子的聚合物。 在某些构型中，重质原子以有效使聚合物不透射线的量存在。 包括HACSCCP的聚合物材料可以制造成用于至少部分地封闭体腔的医疗装置。 例如，这种医疗装置可以是电疗产品。 包括SCC聚合物的聚合物材料也可制成其他医疗装置，例如支架。
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