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1.发明授权
公开(公告)号：US5264345A
公开(公告)日：1993-11-23
申请号：US577480
申请日：1990-09-04
IPC分类号： C07H15/203 , C07H17/00 , C12Q1/40 , G01N33/573 , G01N33/577 , G01N33/53
CPC分类号： C07H15/203 , C07H17/00 , C12Q1/40 , G01N33/573 , C12Q2334/00 , Y10S435/962 , Y10S436/824
摘要： The present invention provides a process for the specific determination of pancreatic .alpha.-amylase in the presence of salivary .alpha.-amylase in body fluids by reaction with a system for the detection of .alpha.-amylase with the use of an inhibitor for salivary .alpha.-amylase, wherein, as substrate, there is used a compound of the general formula: ##STR1## in which R.sub.1 is a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms, a cycloalkyl or cycloalkoxyl radical containing 3 to 6 carbon atoms or a benzoyl, benzyl or phenyl radical which is optionally hydrophilically substituted, R.sub.2 is a hydrogen atom or in which R.sub.1 and R.sub.2 together form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, Gluc is a glucose molecule, n is 1, 2 or 3 and X is an optically determinable residue.The present invention also provides a reagent for the specific determination of pancreatic .alpha.-amylase, as well as new compounds for use in the above process and reagent.
摘要翻译： 本发明提供了一种通过与用于唾液α-淀粉酶抑制剂的α-淀粉酶检测系统的反应来在体液中存在唾液中的α-淀粉酶来特异性测定胰腺α-淀粉酶的方法， 其中，作为底物，使用通式如下的化合物：其中R 1是含有至多6个碳原子的直链或支链烷基或烷酰基，环烷基或环烷氧基，其含有3〜 6个碳原子或任选被亲水取代的苯甲酰基，苄基或苯基，R2是氢原子，或其中R1和R2一起形成亚甲基桥，其氢原子彼此独立地各自被 含有至多5个碳原子的烷基或苯基，Gluc是葡萄糖分子，n是1,2或3，X是光学上可确定的残基。 本发明还提供了用于胰腺α-淀粉酶的特异性测定的试剂，以及用于上述方法和试剂的新化合物。
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2.发明授权Process and reagent for the specific determination of pancreatic .alpha.-amylase 失效用于胰腺α-淀粉酶特异性测定的方法和试剂
公开(公告)号：US5473058A
公开(公告)日：1995-12-05
申请号：US109678
申请日：1993-08-20
IPC分类号： C07H15/203 , C07H17/00 , C12Q1/40 , G01N33/573 , G01N33/577 , C07H15/00
CPC分类号： C07H15/203 , C07H17/00 , C12Q1/40 , G01N33/573 , C12Q2334/00 , Y10S435/962 , Y10S436/824
摘要： The present invention provides a process for the specific determination of pancreatic .alpha.-amylase in the presence of salivary .alpha.-amylase in body fluids by reaction with a system for the detection of .alpha.-amylase with the use of an inhibitor for salivary .alpha.-amylase, wherein, as substrate, there is used a compound of the general formula:-- ##STR1## in which R.sub.1 is a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms, a cycloalkyl or cycloalkoxyl radical containing 3 to 6 carbon atoms or a benzoyl, benzyl or phenyl radical which is optionally hydrophilically substituted, R.sub.2 is a hydrogen atom or in which R.sub.1 and R.sub.2 together form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, n is 1, 2 or 3 and X is an optically determinable residue. The present invention also provides a reagent for the specific determination of pancreatic .alpha.-amylase, as well as new compounds for use in the above process and reagent.
摘要翻译： 本发明提供了一种通过与用于唾液α-淀粉酶抑制剂的α-淀粉酶检测系统的反应来在体液中存在唾液中的α-淀粉酶来特异性测定胰腺α-淀粉酶的方法， 其中，作为底物，使用以下通式的化合物：其中R 1是含有至多6个碳原子的直链或支链烷基或烷酰基，含有3个或更多个碳原子的环烷基或环烷氧基 至6个碳原子或任选被亲水取代的苯甲酰基，苄基或苯基，R 2是氢原子或其中R 1和R 2一起形成亚甲基桥，其氢原子彼此独立地可以被取代 通过含有至多5个碳原子的烷基或苯基，n是1,2或3，X是光学上可确定的残基。 本发明还提供了用于胰腺α-淀粉酶的特异性测定的试剂，以及用于上述方法和试剂的新化合物。
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公开(公告)号：US5506216A
公开(公告)日：1996-04-09
申请号：US231170
申请日：1994-04-22
申请人： Axel Schmidt , Herbert von der Eltz , Klaus Kaluza
发明人： Axel Schmidt , Herbert von der Eltz , Klaus Kaluza
IPC分类号： A61K9/08 , A01N25/10 , A01N25/22 , A01N31/08 , A01N47/48 , A61K47/40 , C08B37/00 , G01N33/52 , C08B37/16 , C09K15/04 , C12Q1/00 , C07K15/06
CPC分类号： A01N25/10 , C08B37/0015 , G01N33/52
摘要： The invention concerns a complex of cyclodextrin and an organic biocide, wherein the organic biocide has a maximum water-solubility of 0.15% (w/v) at a temperature of 25.degree. C., a process for the production of the complex as well as the use of the complex for preserving aqueous solutions. In addition the invention concerns a diagnostic test solution which contains at least one complex according to the invention and a preserved diagnostic test kit which comprises test reagents and at least one complex according to the invention.
摘要翻译： 本发明涉及环糊精和有机杀生物剂的复合物，其中有机杀生物剂在25℃的温度下具有0.15％（w / v）的最大水溶解度，生产复合物的方法以及 使用复合物保存水溶液。 此外，本发明涉及包含至少一种根据本发明的复合物的诊断测试溶液和包含测试试剂和至少一种根据本发明的复合物的保存的诊断测试试剂盒。
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公开(公告)号：US20120164643A1
公开(公告)日：2012-06-28
申请号：US13361549
申请日：2012-01-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q1/6876 , C12Q1/6886 , C12Q2565/1025 , C12Q2563/107
摘要： The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
摘要翻译： 本公开内容涉及针对感兴趣的基因组靶序列的（或互补）部分的一组至少100个单链寡核苷酸探针。 本公开还涉及使用该组寡核苷酸探针检测感兴趣的基因组靶序列的方法和产生寡核苷酸探针组的方法。 此外，本公开涉及包含该组寡核苷酸探针和至少一种另外的组分的试剂盒。
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公开(公告)号：US07501240B2
公开(公告)日：2009-03-10
申请号：US10573215
申请日：2004-12-01
CPC分类号： C07H21/00 , C12Q1/6827 , C12Q2527/125 , C12Q2523/125
摘要： The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.
摘要翻译： 本发明涉及检测核酸中的甲基化胞嘧啶，其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液以及随后的核酸修饰。 由此，将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。
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公开(公告)号：US5198537A
公开(公告)日：1993-03-30
申请号：US427249
申请日：1989-10-25
IPC分类号： A61K39/00 , C07J19/00 , C07J41/00 , C07J43/00 , C07K1/113 , C07K14/00 , C12Q1/68 , G01N33/53 , G01N33/532
CPC分类号： G01N33/532 , C07J19/00 , C07J41/0055 , C07J43/003 , C12Q1/6816 , G01N33/53
摘要： The present invention provides digoxigenin derivatives of the formula: ##STR1## wherein n is a whole number of from 1 to 4 and Z is a carboxyl group or a functional derivative derived therefrom. The present invention also provides digoxigenin conjugates of the general formula: ##STR2## wherein the carrier is an immunogenic carrier material, a nucleic acid or a labelled immunocomponent of a labelled digoxigenin immunoconjugate for use in an immunoassay, m is a number from 1 to the number of coupling positions available on the carrier and Y is the group formed by the reaction of the carboxyl function Z of the digoxigenin derivative of general formula (I) with the reacting position of the carrier material. Also described are uses of these conjugates as nucleic acid probes, immunogens, and in immunoassay.
摘要翻译： 本发明提供下式的地高辛衍生物：其中n为1至4的整数，Z为羧基或衍生自其的官能衍生物。 本发明还提供了以下通式的地高辛缀合物：其中载体是免疫原性载体材料，核酸或免疫缀合物的标记的免疫偶联物的标记的免疫组分，用于免疫测定，m是从 1与载体上可用的偶联位置的数目，Y是通过通式（I）的地高辛衍生物的羧基官能团Z与载体材料的反应位置的反应形成的基团。 还描述了这些缀合物作为核酸探针，免疫原和免疫测定中的用途。
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7.发明授权Process for the detection of compounds containing carbohydrate and a suitable reagent therefor 失效检测含有碳水化合物的化合物及其适用试剂的方法
公开(公告)号：US5179004A
公开(公告)日：1993-01-12
申请号：US454189
申请日：1989-12-21
IPC分类号： G01N33/53 , G01N33/536 , G01N33/543 , G01N33/58 , G01N33/68
CPC分类号： G01N33/68 , G01N33/5308 , G01N33/583 , Y10S435/961
摘要： For the detection of compounds containing carbohydrate the compound to be detected is reacted with a conjugate which contains a group which enables a specific binding to the substance to be detected and contains a hapten with a molecular weight from 300 to 1200, afterwards the complex formed is brought into contact with labelled antibodies directed against the hapten and the label is determined in a known way.
摘要翻译： 为了检测含有碳水化合物的化合物，将要检测的化合物与含有能够与被检测物质特异性结合的基团的缀合物反应，并含有分子量为300〜1200的半抗原，之后形成的复合物为 与针对半抗原的标记抗体接触，并以已知的方式测定标记。
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公开(公告)号：US4900822A
公开(公告)日：1990-02-13
申请号：US135843
申请日：1987-12-21
IPC分类号： C07D265/38 , C07F9/6533 , C07H17/00 , C07H21/00 , C09B19/00 , C09B57/00 , C12Q1/34
CPC分类号： C07H21/00 , C07D265/38 , C07F9/65335 , C07H17/00 , C12Q1/34 , C12Q2304/22 , C12Q2334/00
摘要： Reagents for the determination of hydrolase comprises compounds of the formula ##STR1## wherein Z is an organic or inorganic acid residue or a sugar residue, A is an organic or inorganic acid residue, and B is an organic acid residue. Each of R.sup.2 and R.sup.5, is hydrogen, halogen or lower alkyl. Each of R.sup.1, R.sup.3, R.sup.4 and R.sup.6, is hydrogen, halogen, cyano, lower alkyl, lower alkoxy, carboxyl, lower alkoxycarbonyl, carboxy lower alkyl or lower alkoxycarbonyl lower alkyl, or carboxamido groups optional substituted once or twice, or a radical of the formula--COO--(CH.sub.2 CH.sub.2 O).sub.n --R.sup.7in which R.sup.7 is hydrogen or lower alkyl and n is a number from 1 to 4. Additionally, R.sup.6 can be sulpho or nitro.
摘要翻译： 用于测定水解酶的试剂包括下式的化合物：其中Z是有机或无机酸残基或糖残基，A是有机或无机酸残基，B是有机酸残基。 R 2和R 5各自为氢，卤素或低级烷基。 R 1，R 3，R 4和R 6中的每一个为氢，卤素，氰基，低级烷基，低级烷氧基，羧基，低级烷氧基羰基，羧基低级烷基或低级烷氧基羰基低级烷基或任选取代一次或两次的甲酰胺基， 式-COO-（CH 2 CH 2 O）n -R 7，其中R 7是氢或低级烷基，n是1至4的数。另外，R 6可以是磺基或硝基。
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公开(公告)号：US4615981A
公开(公告)日：1986-10-07
申请号：US663149
申请日：1984-10-22
CPC分类号： C12N9/18 , Y10S435/874
摘要： The present invention provides a process for obtaining cholesterol esterase from micro-organisms by culturing a micro-organism capable of forming cholesterol esterase in an appropriate nutrient medium in the presence of an inductor and obtaining the enzyme from the culture liquid and/or from the cells, therein the inductor used is a compound of the general formula: ##STR1## in which R and R.sub.1 are alkyl or alkoxy radicals containing 14 to 18 carbon atoms and R or R.sub.1 can also be a hydrogen atom and R.sub.2 is an alkylamino radical containing 2 to 8 carbon atoms, an alkyl-trimethylammonium radical containing 3 to 8 carbon atoms, an alkylpyridine radical containing up to 4 carbon atoms in the alkyl moiety or a radical of the general formula --CH.sub.2 --(CHOH).sub.n --CH.sub.2 OH, in which n is a whole number of from 1 to 4.
摘要翻译： 本发明提供从微生物获得胆固醇酯酶的方法，通过在电感器存在下培养能够在合适营养培养基中形成胆固醇酯酶的微生物，并从培养液和/或细胞中获得酶 其中所用的电感是通式为：其中R和R 1为含有14至18个碳原子的烷基或烷氧基的化合物，R或R 1也可以是氢原子，R 2是含有2个烷基氨基的化合物 至8个碳原子，含有3至8个碳原子的烷基 - 三甲基铵基团，烷基部分含有至多4个碳原子的烷基吡啶基团或通式-CH 2 - （CHOH）n -CH 2 OH基团，其中n 是从1到4的整数。
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公开(公告)号：US07951598B2
公开(公告)日：2011-05-31
申请号：US12328890
申请日：2008-12-05
IPC分类号： G01N33/48
CPC分类号： G01N30/06 , G01N30/603 , G01N30/7233 , G01N33/5002 , G01N2030/027 , Y10T436/24 , Y10T436/25
摘要： The invention relates to a method for differentially hemolyzing whole blood. It discloses methods for detecting an analyte in a liquid sample known or suspected to contain red blood cells and suspected or known to contain eukaryotic cells, the method including the steps of processing the liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may contain both erythrocytes and nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.
摘要翻译： 本发明涉及一种使全血不同溶血的方法。 它公开了用于检测已知或怀疑含有红细胞并且怀疑或已知含有真核细胞的液体样品中的分析物的方法，该方法包括在适于裂解细胞膜的条件下用膜增溶剂处理液体样品的步骤 的红细胞，同时不会引起样品成分沉淀，对经处理的样品进行色谱分离，并检测分析物。 在检测可能含有红细胞和有核细胞的液体样品中的分析物的方法中，红细胞的差异溶血是有利的。 红细胞的差异溶解可以容易地与诸如LC-MS的在线检测方法组合，并且在许多分析物的检测中是有利的，例如， 在检测叶酸或免疫抑制药物，如他克莫司或西罗莫司。
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