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公开(公告)号：US09828617B2
公开(公告)日：2017-11-28
申请号：US14421872
申请日：2013-08-13
发明人： Michael Naesby , Christophe Folly , Curt Aimé Friis Nielsen , Anaelle Hatsch , Markus Schwab , Oskar Zelder , Stefan Haefner , Hartwig Schroeder , Birgit Hoff , Andrea Molt , Klaus Ditrich , Michael Breuer , Holger Hartmann , Karsten Koerber
IPC分类号： C12N15/53 , C12P17/12 , C12N9/02 , C12N9/90 , C12N15/52 , C12P17/10 , C12P17/18 , C07D209/90 , C07D487/06 , C07K16/40 , C12N9/10
CPC分类号： C12P17/12 , C07D209/90 , C07D487/06 , C07K16/40 , C12N9/0004 , C12N9/001 , C12N9/1007 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P17/10 , C12P17/182 , C12Y205/01
摘要： Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.
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公开(公告)号：US20150211036A1
公开(公告)日：2015-07-30
申请号：US14421872
申请日：2013-08-13
发明人： Michael Naesby , Christophe Folly , Curt Aimé Friis Nielsen , Anaelle Hatsch , Markus Schwab , Oskar Zelder , Stefan Haefner , Hartwig Schroeder , Birgit Hoff , Andrea Molt , Klaus Ditrich , Michael Breuer , Holger Hartmann , Karsten Koerber
IPC分类号： C12P17/12 , C12N9/02 , C07D209/90 , C12P17/10 , C07K16/40 , C07D487/06 , C12N9/10 , C12N9/90
CPC分类号： C12P17/12 , C07D209/90 , C07D487/06 , C07K16/40 , C12N9/0004 , C12N9/001 , C12N9/1007 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P17/10 , C12P17/182 , C12Y205/01
摘要： Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.
摘要翻译： 用于重组生产角鲨烯型生物碱如环圈子，festuclavine，agroclavine，chanoclavine和chanoclavine醛的微生物和方法，以及多肽，多核苷酸和包含这样的多核苷酸的载体，其可以用于制造棒状的方法 提供生物碱。
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公开(公告)号：US10301588B2
公开(公告)日：2019-05-28
申请号：US15533432
申请日：2015-12-09
申请人： BASF SE
发明人： Birgit Hoff , Stefan Haefner , Weol Kyu Jeong , Edzard Scholten
摘要： The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or biotransformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45° C. to 55° C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA.
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公开(公告)号：US10273485B2
公开(公告)日：2019-04-30
申请号：US15116600
申请日：2015-02-06
申请人： BASF SE
发明人： Joanna Martyna Krawczyk , Stefan Haefner , Hartwig Schröder , Esther Dantas Costa , Oskar Zelder , Gregory Von Abendroth , Christoph Wittmann , René Stellmacher , Judith Becker
摘要： The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.
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5.发明授权Genetically modified microorganisms capable of producing beta-glucans and methods for producing beta-glucans 有权能够产生β-葡聚糖的转基因微生物和生产β-葡聚糖的方法公开(公告)号：US09322041B2
公开(公告)日：2016-04-26
申请号：US13935043
申请日：2013-07-03
申请人： BASF SE , Wintershall Holding GmbH
发明人： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC分类号： C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
摘要翻译： 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于，遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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公开(公告)号：US20160304917A1
公开(公告)日：2016-10-20
申请号：US14914855
申请日：2014-08-18
申请人： BASF SE
发明人： Joanna Martyna Krawczyk , Stefan Haefner , Hartwig Schröder , Oskar Zelder , Jonathan Thomas Fabarius
CPC分类号： C12P13/06 , C12N9/0016 , C12R1/01 , C12Y104/01001
摘要： The present invention relates to a modified microorganism having, compared to its wildtype, an increased activity of the enzyme that is encoded by the alaD-gene. The present invention also relates to a method for producing an alanine and to the use of modified microorganisms.
摘要翻译： 本发明涉及一种经修饰的微生物，其与野生型相比具有增加由alaD基因编码的酶的活性。 本发明还涉及丙氨酸的生产方法和改性微生物的使用。
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7.发明授权Genetically modified microorganisms capable of producing β-glucans and methods for producing β-glucans 有权能够生产葡聚糖的转基因微生物和生产葡聚糖的方法公开(公告)号：US09388441B1
公开(公告)日：2016-07-12
申请号：US15074330
申请日：2016-03-18
申请人： BASF SE , Wintershall Holding GmbH
发明人： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC分类号： C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
摘要翻译： 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于，遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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公开(公告)号：US11299522B2
公开(公告)日：2022-04-12
申请号：US15779595
申请日：2016-12-02
申请人： BASF SE
摘要： The present invention relates to a method of producing a recombinant polypeptide a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 2 (CLR2) and to express said recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR2 and the use of this filamentous fungus in the production of a recombinant polypeptide.
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公开(公告)号：US11279955B2
公开(公告)日：2022-03-22
申请号：US16614772
申请日：2018-05-18
申请人： BASF SE
发明人： Hartwig Schroeder , Stefan Haefner , Oskar Zelder , Christoph Wittmann , Anna Christine Schroer , Birgit Hoff , Nico Boehmer
摘要： Described herein is a process of producing an organic compound, the process including: I) cultivating a genetically modified microorganism in a culture medium including sucrose as an assimilable carbon source to allow the genetically modified microorganism to produce the organic compound, and II) recovering the organic compound from the fermentation broth obtained in process step I) The genetically modified microorganism includes A) at least one genetic modification that leads to an increased activity of the enzyme encoded by the rbsK-gene, compared to the original microorganism that has not been genetically modified, and the original microorganism belongs to the family Pasteurellaceae. Also described herein are a genetically modified microorganism and the use thereof for the fermentative production of an organic compound from sucrose as an assimilable carbon source.
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公开(公告)号：US10287571B2
公开(公告)日：2019-05-14
申请号：US15308874
申请日：2015-05-07
申请人： BASF SE
IPC分类号： A61K38/00 , C12N15/10 , C12P7/46 , C07K14/195 , C12N15/74
摘要： The present invention relates to a modified microorganism having, compared to its wildtype, —a reduced activity of an enzyme encoded by the ptsA-gene, —a reduced activity of an enzyme encoded by the ptsH-gene or —a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.
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