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1.发明申请公开(公告)号：US20120009640A1
公开(公告)日：2012-01-12
申请号：US13171138
申请日：2011-06-28
申请人： Behnaz Behrouzian , Louis Clark , Yihui Zhu , Michael Clay , Kristian Karlshoej
发明人： Behnaz Behrouzian , Louis Clark , Yihui Zhu , Michael Clay , Kristian Karlshoej
IPC分类号： C12P7/04 , C12N1/19 , C12N1/21 , C11D3/386 , C07C1/22 , C12P5/02 , C07C31/125 , C07C69/003 , C11D3/22 , C11D3/60 , C12N9/06 , C07C67/00
CPC分类号： C12N9/0008 , C07C1/22 , C07K2319/00 , C07K2319/036 , C10G3/00 , C10G3/40 , C10G2300/1011 , C10G2400/04 , C11D3/2003 , C11D3/2013 , C11D3/2031 , C12N9/0006 , C12P7/04 , Y02P30/20
摘要： The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.
摘要翻译： 本公开内容提供了用于从重组宿主细胞产生脂肪醇组合物的方法。 本公开进一步提供变体脂肪酰基-CoA还原酶（FAR）酶，编码变体FAR酶的多核苷酸，以及载体和包含其的宿主细胞。
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公开(公告)号：US08574878B2
公开(公告)日：2013-11-05
申请号：US13171138
申请日：2011-06-28
申请人： Behnaz Behrouzian , Louis Clark , Yihui Zhu , Michael Clay , Kristian Karlshoej
发明人： Behnaz Behrouzian , Louis Clark , Yihui Zhu , Michael Clay , Kristian Karlshoej
IPC分类号： C12P7/04 , C12P5/02 , C12P7/42 , C12P21/06 , C12N9/02 , C12N1/20 , C12N1/00 , C11D3/00 , C07C51/00 , C07C53/00 , C07C1/00 , C07C29/00
CPC分类号： C12N9/0008 , C07C1/22 , C07K2319/00 , C07K2319/036 , C10G3/00 , C10G3/40 , C10G2300/1011 , C10G2400/04 , C11D3/2003 , C11D3/2013 , C11D3/2031 , C12N9/0006 , C12P7/04 , Y02P30/20
摘要： The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.
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3.发明授权Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR) 有权用脂肪醇形成酰基辅酶A还原酶（FAR）生产脂肪醇公开(公告)号：US08216815B2
公开(公告)日：2012-07-10
申请号：US12825939
申请日：2010-06-29
IPC分类号： C12P7/42 , C12P21/06 , C12P21/04 , C12Q1/68 , C12Q1/26 , C12N9/02 , C12N1/20 , C12N15/00 , C07H21/04
摘要： The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
摘要翻译： 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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4.发明申请PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR) 有权生产脂肪醇与脂肪醇形成ACYA-COA还原剂（FAR）公开(公告)号：US20110000125A1
公开(公告)日：2011-01-06
申请号：US12825939
申请日：2010-06-29
IPC分类号： C10L1/19 , C12P7/64 , C07H21/00 , C12N15/63 , C12N1/21 , C12N1/19 , C12N9/02 , C12P5/00 , C07C9/00 , C09K3/00
摘要： The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
摘要翻译： 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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公开(公告)号：US20100298612A1
公开(公告)日：2010-11-25
申请号：US12784770
申请日：2010-05-21
申请人： Behnaz Behrouzian , Robert McDaniel , Xiyun Zhang , Louis Clark
发明人： Behnaz Behrouzian , Robert McDaniel , Xiyun Zhang , Louis Clark
CPC分类号： C12P7/04 , C12N9/0008 , C12N9/1288
摘要： The present disclosure provides a process for the production of long chain fatty alcohols by recombinant host cells expressing one or more heterologous carboxylic acid reductase enzymes useful for the conversion of fatty acids, and derivatives thereof, to long chain fatty alcohols.
摘要翻译： 本公开提供了通过表达用于将脂肪酸及其衍生物转化成长链脂肪醇的一种或多种异源羧酸还原酶的重组宿主细胞生产长链脂肪醇的方法。
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6.发明授权Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR) 有权用脂肪醇形成酰基辅酶A还原酶（FAR）生产脂肪醇公开(公告)号：US08574877B2
公开(公告)日：2013-11-05
申请号：US13494709
申请日：2012-06-12
IPC分类号： C12P7/42 , C12P21/06 , C12P21/04 , C12Q1/68 , C12Q1/26 , C12N9/02 , C12N1/20 , C12N15/00 , C07H21/04
摘要： The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
摘要翻译： 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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7.发明申请PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR) 有权生产脂肪醇与脂肪醇形成ACYA-COA还原剂（FAR）公开(公告)号：US20130040352A1
公开(公告)日：2013-02-14
申请号：US13494709
申请日：2012-06-12
摘要： The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
摘要翻译： 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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8.发明授权Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose 有权使用具有改善热稳定性的糖水解酶61蛋白质变体来加工纤维素公开(公告)号：US09493802B2
公开(公告)日：2016-11-15
申请号：US13640471
申请日：2011-08-22
申请人： Jie Yang , Louis Clark , Onorato Campopiano , Kripa Rao , Lorand Szabo , Janos Torok , Dipnath Baidyaroy
发明人： Jie Yang , Louis Clark , Onorato Campopiano , Kripa Rao , Lorand Szabo , Janos Torok , Dipnath Baidyaroy
CPC分类号： C12P19/02 , C12N9/242 , C12N9/2434 , C12P7/10 , C12P7/14 , C12P19/14 , Y02E50/16 , Y02P20/52
摘要： The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.
摘要翻译： 本发明提供了从嗜热丝霉菌（Myceliophtora thermophila）获得的重组GH61蛋白质和编码这种蛋白质的核酸。 本发明还提供从具有GH61蛋白活性的嗜热绵羊嗜热上清液分离的蛋白质级分。 可以使用这些制剂从含有纤维素的底物通过一种或多种纤维素酶如内切葡聚糖酶，β-葡糖苷酶或纤维二糖水解酶进行糖化的反应来提高产品的产量。 GH61蛋白和纤维素酶的组合可用于在生产乙醇中将纤维素生物质分解成可发酵糖。
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公开(公告)号：US08652813B2
公开(公告)日：2014-02-18
申请号：US12950150
申请日：2010-11-19
申请人： Dipnath Baidyaroy , Louis Clark , Lisa M. Newman
发明人： Dipnath Baidyaroy , Louis Clark , Lisa M. Newman
IPC分类号： C12P19/02
CPC分类号： C12N9/2445 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present invention provides compositions and methods for the expression of recombinant β-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
摘要翻译： 本发明提供了用于表达重组β-葡糖苷酶变体的组合物和方法，以及它们在从纤维素生物质生产可发酵糖中的用途。
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公开(公告)号：US08357523B2
公开(公告)日：2013-01-22
申请号：US12816989
申请日：2010-06-16
IPC分类号： C12N9/26
CPC分类号： C12N9/42 , C12N9/2445 , C12P7/06 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The invention provides variants of the Azospirillum irakense CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
摘要翻译： 本发明提供了与野生型酶相比具有改善的β-葡糖苷酶活性，特别是改进的热活性的Azospirillum irakense CelA和葡糖苷酶的变体。 本发明还提供了相关的多核苷酸，载体，宿主细胞，以及用于制备和使用变体的方法。
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