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公开(公告)号：US06875607B1
公开(公告)日：2005-04-05
申请号：US09436164
申请日：1999-11-09
申请人： Benjamin Eithan Reubinoff , Martin Frederick Pera , Chui-Yee Fong , Alan Osborne Trounson , Ariffeen Bongso
发明人： Benjamin Eithan Reubinoff , Martin Frederick Pera , Chui-Yee Fong , Alan Osborne Trounson , Ariffeen Bongso
IPC分类号： C12N15/09 , A61K35/12 , C12N5/02 , C12N5/0735 , C12N5/077 , C12N5/10 , C12R1/91 , A01N1/00 , C12N5/00
CPC分类号： C12N5/0652 , A61K35/12 , C12N5/0606 , C12N2502/13 , C12N2506/02
摘要： The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation, production of differentiated cells and in particular the production of human embryonic stem cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells capable of giving rise to mature somatic cells and uses thereof. The present invention also provides a purified preparation of undifferentiated human embryonic stem cells capable of proliferation in vitro. Furthermore, the present invention provides a somatic cell differentiated in vitro from an undifferentiated embryonic stem cell. There is also provided a committed progenitor cell capable of giving rise to mature somatic cells.
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公开(公告)号：US20090075373A1
公开(公告)日：2009-03-19
申请号：US12157864
申请日：2008-06-13
IPC分类号： C12N5/08
CPC分类号： C12N5/0623 , A61K35/12 , C12N5/0606 , C12N5/0619 , C12N5/0622 , C12N2500/32 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/91 , C12N2502/13 , C12N2506/02
摘要： The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof.This invention provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and cells that are generated along the pathway of neural differentiation may be used for: the study of the cellular and molecular biology of human neural development, discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, drug discovery and the development of screening assays for teratogenic, toxic and neuroprotective effects.
摘要翻译： 本发明涉及未分化的人类胚胎干细胞，培养和繁殖方法以及分化细胞的产生。 特别地，它涉及能够在体外产生体细胞分化细胞的人ES细胞的产生，以及确定的祖细胞，例如能够产生包括神经细胞和/或胶质细胞的成熟体细胞的神经祖细胞及其用途 。 本发明提供了产生ES细胞向神经谱系的受控分化的体外和体内模型的方法。 沿着神经分化途径产生的模型和细胞可用于：人类神经发育的细胞和分子生物学的研究，发现基因，生长因子和在神经分化中发挥作用的分化因子， 再生，药物发现和开发用于致畸，毒性和神经保护作用的筛选试验。
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公开(公告)号：US08137969B2
公开(公告)日：2012-03-20
申请号：US12157864
申请日：2008-06-13
CPC分类号： C12N5/0623 , A61K35/12 , C12N5/0606 , C12N5/0619 , C12N5/0622 , C12N2500/32 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/91 , C12N2502/13 , C12N2506/02
摘要： The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof.
摘要翻译： 本发明涉及未分化的人类胚胎干细胞，培养和繁殖方法以及分化细胞的产生。 特别地，它涉及能够在体外产生体细胞分化细胞的人ES细胞的产生，以及确定的祖细胞，例如能够产生包括神经细胞和/或胶质细胞的成熟体细胞的神经祖细胞及其用途 。
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4.发明授权公开(公告)号：US07011828B2
公开(公告)日：2006-03-14
申请号：US09970543
申请日：2001-10-04
CPC分类号： C12N5/0623 , A61K35/12 , C12N5/0606 , C12N5/0619 , C12N5/0622 , C12N2500/32 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/395 , C12N2501/91 , C12N2502/13 , C12N2506/02
摘要： The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof.This invention provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and cells that are generated along the pathway of neural differentiation may be used for: the study of the cellular and molecular biology of human neural development, discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, drug discovery and the development of screening assays for teratogenic, toxic and neuroprotective effects.
摘要翻译： 本发明涉及未分化的人类胚胎干细胞，培养和繁殖方法以及分化细胞的产生。 特别地，它涉及能够在体外产生体细胞分化细胞的人ES细胞的产生，以及确定的祖细胞，例如能够产生包括神经细胞和/或胶质细胞的成熟体细胞的神经祖细胞及其用途 。 本发明提供了产生ES细胞向神经谱系的受控分化的体外和体内模型的方法。 沿着神经分化途径产生的模型和细胞可用于：人类神经发育的细胞和分子生物学的研究，发现基因，生长因子和在神经分化中发挥作用的分化因子， 再生，药物发现和开发用于致畸，毒性和神经保护作用的筛选试验。
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公开(公告)号：US07947498B2
公开(公告)日：2011-05-24
申请号：US12367075
申请日：2009-02-06
CPC分类号： C12N5/0623 , A61K35/12 , C12N5/0606 , C12N5/0619 , C12N5/0622 , C12N2500/32 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/91 , C12N2502/13 , C12N2506/02
摘要： The present invention provides undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, and committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. The invention also provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and the cells that are generated along the pathway of neural differentiation may be used for the study of the cellular and molecular biology of human neural development, for the discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, for drug discovery and for the development of screening assays for teratogenic, toxic and neuroprotective effects.
摘要翻译： 本发明提供未分化的人胚胎干细胞，培养和繁殖方法以及分化细胞的产生。 特别涉及能够在体外产生体细胞分化细胞的人类ES细胞的产生，以及定向的祖细胞，例如能够产生包括神经细胞和/或胶质细胞的成熟体细胞的神经祖细胞及其用途。 本发明还提供了产生ES细胞向神经谱系的受控分化的体外和体内模型的方法。 该模型和沿着神经分化途径产生的细胞可用于研究人神经发育的细胞和分子生物学，用于发现在神经中发挥作用的基因，生长因子和分化因子 分化和再生，用于药物发现和开发用于致畸，毒性和神经保护作用的筛选测定。
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公开(公告)号：US07504257B2
公开(公告)日：2009-03-17
申请号：US09808382
申请日：2001-03-14
CPC分类号： C12N5/0623 , A61K35/12 , C12N5/0606 , C12N5/0619 , C12N5/0622 , C12N2500/32 , C12N2501/11 , C12N2501/115 , C12N2501/135 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/91 , C12N2502/13 , C12N2506/02
摘要： The present invention provides undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, and committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. The invention also provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and the cells that are generated along the pathway of neural differentiation may be used for the study of the cellular and molecular biology of human neural development, for the discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, for drug discovery and for the development of screening assays for teratogenic, toxic and neuroprotective effects.
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公开(公告)号：US07413903B2
公开(公告)日：2008-08-19
申请号：US10657703
申请日：2003-09-09
CPC分类号： G01N33/6872 , C12N5/0606 , C12N2500/25 , C12N2500/36 , C12N2500/90 , C12N2501/13 , C12N2501/135 , C12N2501/25 , C12N2506/00 , G01N2333/49 , G01N2500/04
摘要： The present invention provides methods, media and compositions capable of modulating the differentiation of stem cells. Applicants have discovered that agonists of lysophospholipid receptors and ligands of class III tyrosine kinase receptors are useful in preventing the spontaneous differentiation of stem cells. The ligands and agonists may be used alone, or in combination where they have a synergistic effect. Also provided are cells produced using the methods and media, and methods of treating stem cell related diseases using the compositions described herein. Methods of identifying compounds useful in finding other agents useful in the modulation of stem cell differentiation are also disclosed.
摘要翻译： 本发明提供能够调节干细胞分化的方法，介质和组合物。 申请人已经发现溶血磷脂受体的激动剂和III类酪氨酸激酶受体的配体可用于预防干细胞的自发分化。 配体和激动剂可以单独使用，也可以组合使用，因为它们具有协同效应。 还提供了使用方法和培养基产生的细胞，以及使用本文所述的组合物治疗干细胞相关疾病的方法。 还公开了鉴定可用于发现可用于调节干细胞分化的其它试剂的化合物的方法。
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公开(公告)号：US07604990B2
公开(公告)日：2009-10-20
申请号：US11006300
申请日：2004-12-07
CPC分类号： G01N33/6872 , C12N5/0606 , C12N2500/25 , C12N2500/36 , C12N2500/90 , C12N2501/13 , C12N2501/135 , C12N2501/25 , C12N2506/00 , G01N2333/49 , G01N2500/04
摘要： The present invention provides methods, media and compositions capable of modulating the differentiation of stem cells. Applicants have discovered that agonists of lysophospholipid receptors and ligands of class III tyrosine kinase receptors are useful in preventing the spontaneous differentiation of stem cells. The ligands and agonists may be used alone, or in combination where they have a synergistic effect. Also provided are cells produced using the methods and media, and methods of treating stem cell related diseases using the compositions described herein. Methods of identifying compounds useful in finding other agents useful in the modulation of stem cell differentiation are also disclosed.
摘要翻译： 本发明提供能够调节干细胞分化的方法，介质和组合物。 申请人已经发现溶血磷脂受体的激动剂和III类酪氨酸激酶受体的配体可用于预防干细胞的自发分化。 配体和激动剂可以单独使用，也可以组合使用，因为它们具有协同效应。 还提供了使用方法和培养基产生的细胞，以及使用本文所述的组合物治疗干细胞相关疾病的方法。 还公开了鉴定可用于发现可用于调节干细胞分化的其它试剂的化合物的方法。
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公开(公告)号：US20090197334A1
公开(公告)日：2009-08-06
申请号：US12363194
申请日：2009-01-30
IPC分类号： C12N5/08
CPC分类号： C12N5/0606 , C12N5/0603 , C12N2501/13 , C12N2501/155 , C12N2501/33 , C12N2502/088 , C12N2506/02
摘要： The present invention provides a preparation of undifferentiated embryonic stem (ES) cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Preferably the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extraembryonic lineage. The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention.
摘要翻译： 本发明提供了未分化胚胎干细胞（ES）细胞在未分化状态下持续延长的制剂，其将经历干细胞更新或体细胞分化。 优选地，细胞在体外能够体细胞分化，并倾向于远离胚外谱系分化。 本发明还提供培养胚胎干（ES）细胞以改善培养中的干细胞维持和持久性的方法。 该方法还提供了通过该方法制备的ES细胞的培养物，以及源自由本发明提供的定向分化程序产生的胚胎细胞的分化细胞。
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10.发明授权Culturing human embryonic stem cells with a noggin to generate cells lacking Pax-6 expression 有权用头蛋白培养人胚胎干细胞以产生缺乏Pax-6表达的细胞公开(公告)号：US09080147B2
公开(公告)日：2015-07-14
申请号：US12363194
申请日：2009-01-30
IPC分类号： C12N5/00 , C12N5/0735 , C12N5/073
CPC分类号： C12N5/0606 , C12N5/0603 , C12N2501/13 , C12N2501/155 , C12N2501/33 , C12N2502/088 , C12N2506/02
摘要： The present invention provides a preparation of undifferentiated embryonic stem (ES) cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Preferably the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extraembryonic lineage. The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention.
摘要翻译： 本发明提供了未分化胚胎干细胞（ES）细胞在未分化状态下持续延长的制剂，其将经历干细胞更新或体细胞分化。 优选地，细胞在体外能够体细胞分化，并倾向于远离胚外谱系分化。 本发明还提供培养胚胎干（ES）细胞以改善培养中的干细胞维持和持久性的方法。 该方法还提供了通过该方法制备的ES细胞的培养物，以及源自由本发明提供的定向分化程序产生的胚胎细胞的分化细胞。
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