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    High-speed thermal cycling system and method of use
    10.
    发明授权
    High-speed thermal cycling system and method of use 失效
    高速热循环系统及其使用方法

    公开(公告)号：US5508197A

    公开(公告)日：1996-04-16

    申请号：US279412

    申请日：1994-07-25

    申请人： Anthony D. A. Hansen ,  Joseph M. Jaklevic

    发明人： Anthony D. A. Hansen ,  Joseph M. Jaklevic

    IPC分类号： B01L7/00 ,  C12M1/38 ,  C12M1/36

    CPC分类号： B01L7/52 ,  B01L2300/0893 ,  Y10S435/809

    摘要： A thermal cycling system and method of use are described. The thermal cycling system is based on the-circulation of temperature-controlled water directly to the underside of thin-walled polycarbonate microtiter plates. The water flow is selected from a manifold fed by pumps from heated reservoirs. The plate wells are loaded with typically 15-20 .mu.l of reagent mix for the PCR process. Heat transfer through the thin polycarbonate is sufficiently rapid that the contents reach thermal equilibrium with the water in less than 15 seconds. Complete PCR amplification runs of 40 three-step cycles have been performed in as little as 14.5 minutes, with the results showing substantially enhanced specificity compared to conventional technology requiring run times in excess of 100 minutes. The plate clamping station is designed to be amenable to robotic loading and unloading of the system. It includes a heated lid, thus eliminating the need for mineral oil overlay of the reactants. The present system includes three or more plate holder stations, fed from common reservoirs but operating with independent switching cycles. The system can be modularly expanded.

    摘要翻译： 描述了一种热循环系统和使用方法。 热循环系统基于将温度控制的水直接循环到薄壁聚碳酸酯微量滴定板的底部。 水流从加热储存器的泵供给的歧管中选择。 加载平板孔通常用于PCR过程的15-20μl试剂混合物。 通过薄聚碳酸酯的热传递足够快，使得内容物在少于15秒内与水达到热平衡。 在短短14.5分钟内进行了40个三步循环的完全PCR扩增，结果显示出与需要超过100分钟的运行时间的常规技术相比，显着提高了特异性。 夹板站设计为适合于系统的机器人装载和卸载。 它包括加热盖，因此不需要反应物的矿物油覆盖。 本系统包括三个或更多个板夹持器站，其由公共储存器供给，但是以独立的开关周期运行。 该系统可以模块化地扩展。