-
1.发明授权Integrated crystal mounting and alignment system for high-throughput biological crystallography 失效用于高通量生物晶体学的集成晶体安装和对准系统公开(公告)号：US06918698B2
公开(公告)日：2005-07-19
申请号：US10319282
申请日：2002-12-12
申请人： Robert A. Nordmeyer , Gyorgy P. Snell , Earl W. Cornell , William Kolbe , Derek Yegian , Thomas N. Earnest , Joseph M. Jaklevic , Carl W. Cork , Bernard D. Santarsiero , Raymond C. Stevens
发明人： Robert A. Nordmeyer , Gyorgy P. Snell , Earl W. Cornell , William Kolbe , Derek Yegian , Thomas N. Earnest , Joseph M. Jaklevic , Carl W. Cork , Bernard D. Santarsiero , Raymond C. Stevens
CPC分类号： G01N23/20025 , B01L9/065 , B01L2200/18 , B01L2300/1883 , C30B29/58 , C30B33/00 , C30B35/005 , G01N1/42 , G01N23/207
摘要： A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.
摘要翻译： 用于运输，远程和无人值守安装的方法和装置，以及用于同步加速器产生的x射线衍射分析的蛋白质晶体的视觉对准和监测。 蛋白质样品始终保持在液氮温度：装运期间，安装，安装，对准，数据采集和拆卸之后。 样品必须在空间的某一点处另外稳定地对准至几微米。 远程和自动地准确执行这些任务的能力导致大量蛋白质表征工作的样品通量和可靠性的显着增加。 由于将蛋白质样品放置在每个容纳多个样品的样品盒的运输兼容层叠堆叠中，因此大量样品可以在单个低温运输容器中运输。
-
2.发明授权Integrated crystal mounting and alignment system for high-throughput biological crystallography 失效用于高通量生物晶体学的集成晶体安装和对准系统公开(公告)号：US07274769B2
公开(公告)日：2007-09-25
申请号：US11064357
申请日：2005-02-22
申请人： Robert A. Nordmeyer , Gyorgy P. Snell , Earl W. Cornell , William F. Kolbe , Derek T. Yegian , Thomas N. Earnest , Joseph M. Jaklevich , Carl W. Cork , Bernard D. Santarsiero , Raymond C. Stevens
发明人： Robert A. Nordmeyer , Gyorgy P. Snell , Earl W. Cornell , William F. Kolbe , Derek T. Yegian , Thomas N. Earnest , Joseph M. Jaklevich , Carl W. Cork , Bernard D. Santarsiero , Raymond C. Stevens
IPC分类号： G01N23/20
CPC分类号： G01N23/20025 , B01L9/065 , B01L2200/18 , B01L2300/1883 , C30B29/58 , C30B33/00 , C30B35/005 , G01N1/42 , G01N23/207
摘要： A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.
摘要翻译： 用于运输，远程和无人值守安装的方法和装置，以及用于同步加速器产生的x射线衍射分析的蛋白质晶体的视觉对准和监测。 蛋白质样品始终保持在液氮温度：装运期间，安装，安装，对准，数据采集和拆卸之后。 样品必须在空间的某一点处另外稳定地对准至几微米。 远程和自动地准确执行这些任务的能力导致大量蛋白质表征工作的样品通量和可靠性的显着增加。 由于将蛋白质样品放置在每个容纳多个样品的样品盒的运输兼容层叠堆叠中，因此大量样品可以在单个低温运输容器中运输。
-
3.发明授权公开(公告)号：US07001438B2
公开(公告)日：2006-02-21
申请号：US10119332
申请日：2002-04-08
申请人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
发明人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00328 , B01J2219/00376 , B01J2219/00378 , B01J2219/00412 , B01J2219/00547 , B01J2219/00585 , B01J2219/0065 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00725 , B01J2219/00756 , B01L3/021 , B01L3/5085 , B01L3/50851 , B01L3/5088 , B01L2300/021 , B01L2300/04 , B01L2300/0609 , B01L2300/0829 , B01L2300/10 , B01L2400/0478 , B01L2400/0633 , C07K2299/00 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N33/68 , G01N2001/4027 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A method is provided for forming crystallization experiments for a molecule, the method comprising forming a plurality of crystallization experiments that comprise a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments, the crystallization experiments having a volume less than 1 microliter, the volumes of the plurality of crystallization experiments varying among the experiments by less than 25 nL.
摘要翻译： 提供了一种用于形成分子的结晶实验的方法，该方法包括形成包含待结晶分子的多个结晶实验和在多个结晶实验中变化的组合物，结晶实验具有小于1微升的体积 ，多个结晶实验的体积在实验之间变化小于25nL。
-
公开(公告)号：US06932845B2
公开(公告)日：2005-08-23
申请号：US10119187
申请日：2002-04-08
申请人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
发明人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
IPC分类号： G01N33/68 , B01D9/02 , B01J19/00 , B01L3/00 , B01L3/02 , C07K1/00 , C12M1/34 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N1/40 , B01D9/00 , G01N33/00
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00328 , B01J2219/00376 , B01J2219/00378 , B01J2219/00412 , B01J2219/00547 , B01J2219/00585 , B01J2219/0065 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00725 , B01J2219/00756 , B01L3/021 , B01L3/5085 , B01L3/50851 , B01L3/5088 , B01L2300/021 , B01L2300/04 , B01L2300/0609 , B01L2300/0829 , B01L2300/10 , B01L2400/0478 , B01L2400/0633 , C07K2299/00 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N33/68 , G01N2001/4027 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A method is provided for performing crystallization experiments for a molecule, the method comprising performing a plurality of crystallization experiments where the crystallization experiments have volumes of less than 1 microliter, the crystallization experiments comprising a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments detecting crystal formation in the crystallization experiments.
摘要翻译： 提供了一种用于对分子进行结晶实验的方法，所述方法包括进行多个结晶实验，其中结晶实验的体积小于1微升，结晶实验包括待结晶的分子和在多个结晶中变化的组合物 的结晶实验检测结晶实验中的晶体形成。
-
5.发明授权Automated method for setting up multiple crystallization experiments in submicroliter volumes 有权用于在亚微米体积中设置多个结晶实验的自动化方法公开(公告)号：US07015041B2
公开(公告)日：2006-03-21
申请号：US10334336
申请日：2002-12-31
申请人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
发明人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
IPC分类号： G01N31/00
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00328 , B01J2219/00376 , B01J2219/00378 , B01J2219/00412 , B01J2219/00547 , B01J2219/00585 , B01J2219/0065 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00725 , B01J2219/00756 , B01L3/021 , B01L3/5085 , B01L3/50851 , B01L3/5088 , B01L2300/021 , B01L2300/04 , B01L2300/0609 , B01L2300/0829 , B01L2300/10 , B01L2400/0478 , B01L2400/0633 , C07K2299/00 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N33/68 , G01N2001/4027 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.
摘要翻译： 提供了一种用于进行阵列微结晶以确定分子的合适结晶条件的方法，所述方法包括：形成微结晶阵列，每个微晶化包括包含其阵列内组成变化的母液溶液和待结晶的分子的液滴， 该液滴体积小于1微升; 在适合于分子晶体的条件下存储微结晶阵列以在阵列中的液滴中形成; 并通过拍摄液滴的图像来检测液滴中的分子晶体形成。
-
公开(公告)号：US06630006B2
公开(公告)日：2003-10-07
申请号：US09851397
申请日：2001-05-07
申请人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
发明人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
IPC分类号： B01D900
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00328 , B01J2219/00376 , B01J2219/00378 , B01J2219/00412 , B01J2219/00547 , B01J2219/00585 , B01J2219/0065 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00725 , B01J2219/00756 , B01L3/021 , B01L3/5085 , B01L3/50851 , B01L3/5088 , B01L2300/021 , B01L2300/04 , B01L2300/0609 , B01L2300/0829 , B01L2300/10 , B01L2400/0478 , B01L2400/0633 , C07K2299/00 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N33/68 , G01N2001/4027 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.
-
公开(公告)号：US20080181834A1
公开(公告)日：2008-07-31
申请号：US12003375
申请日：2007-12-21
申请人： Bernard D. Santarsiero , Derek T. Yegian , Robert A. Nordmeyer , Earl W. Cornell , Joseph M. Jaklevic , Peter G. Schultz , Raymond C. Stevens
发明人： Bernard D. Santarsiero , Derek T. Yegian , Robert A. Nordmeyer , Earl W. Cornell , Joseph M. Jaklevic , Peter G. Schultz , Raymond C. Stevens
IPC分类号： B01D9/00
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00328 , B01J2219/00376 , B01J2219/00378 , B01J2219/00412 , B01J2219/00547 , B01J2219/00585 , B01J2219/0065 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00725 , B01J2219/00756 , B01L3/5085 , B01L3/50851 , B01L3/5088 , B01L2300/021 , B01L2300/04 , B01L2300/0609 , B01L2300/0829 , B01L2300/10 , B01L2400/0478 , B01L2400/0633 , C07K2299/00 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N2001/4027 , Y10T436/25
摘要： A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.
摘要翻译： 提供了一种用于进行阵列微结晶以确定分子的合适结晶条件的方法，所述方法包括：形成微结晶阵列，每个微晶化包括包含其阵列内组成变化的母液溶液和待结晶的分子的液滴， 该液滴体积小于1微升; 在适合于分子晶体的条件下存储微结晶阵列以在阵列中的液滴中形成; 并通过拍摄液滴的图像来检测液滴中的分子晶体形成。
-
8.发明授权公开(公告)号：US06951575B2
公开(公告)日：2005-10-04
申请号：US10324022
申请日：2002-12-18
申请人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
发明人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
IPC分类号： G01N33/68 , B01D9/02 , B01J19/00 , B01L3/00 , B01L3/02 , C07K1/00 , C12M1/34 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N1/40 , B01D9/00 , G01N33/00
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00328 , B01J2219/00376 , B01J2219/00378 , B01J2219/00412 , B01J2219/00547 , B01J2219/00585 , B01J2219/0065 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00725 , B01J2219/00756 , B01L3/021 , B01L3/5085 , B01L3/50851 , B01L3/5088 , B01L2300/021 , B01L2300/04 , B01L2300/0609 , B01L2300/0829 , B01L2300/10 , B01L2400/0478 , B01L2400/0633 , C07K2299/00 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N33/68 , G01N2001/4027 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.
摘要翻译： 提供了一种用于进行阵列微结晶以确定分子的合适结晶条件的方法，所述方法包括：形成微结晶阵列，每个微晶化包括包含其阵列内组成变化的母液溶液和待结晶的分子的液滴， 该液滴体积小于1微升; 在适合于分子晶体的条件下存储微结晶阵列以在阵列中的液滴中形成; 并通过拍摄液滴的图像来检测液滴中的分子晶体形成。
-
9.发明授权Method for diffracting crystals formed by submicroliter crystallization experiments 失效衍射通过亚微米结晶实验形成的晶体的方法公开(公告)号：US06911056B2
公开(公告)日：2005-06-28
申请号：US10119237
申请日：2002-04-08
申请人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
发明人： Bernard D. Santarsiero , Raymond C. Stevens , Peter G. Schultz , Joseph M. Jaklevic , Derek T. Yegian , Earl W. Cornell , Robert A. Nordmeyer
IPC分类号： G01N33/68 , B01D9/02 , B01J19/00 , B01L3/00 , B01L3/02 , C07K1/00 , C12M1/34 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N1/40 , B01D9/00 , G01N33/00
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00313 , B01J2219/00317 , B01J2219/00328 , B01J2219/00376 , B01J2219/00378 , B01J2219/00412 , B01J2219/00547 , B01J2219/00585 , B01J2219/0065 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00725 , B01J2219/00756 , B01L3/021 , B01L3/5085 , B01L3/50851 , B01L3/5088 , B01L2300/021 , B01L2300/04 , B01L2300/0609 , B01L2300/0829 , B01L2300/10 , B01L2400/0478 , B01L2400/0633 , C07K2299/00 , C30B7/00 , C30B29/58 , C40B40/10 , C40B60/14 , C40B70/00 , G01N33/68 , G01N2001/4027 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： A method is provided for characterizing a crystal structure of a molecule, the method comprising performing a plurality of crystallization experiments where the crystallization experiments have volumes of less than 1 microliter, the crystallization experiments comprising a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments and diffracting a crystal formed in one of the crystallization experiments.
摘要翻译： 提供用于表征分子的晶体结构的方法，该方法包括进行多次结晶实验，其中结晶实验的体积小于1微升，结晶实验包括待结晶的分子和在 多个结晶实验和衍射在一个结晶实验中形成的晶体。
-
10.发明申请APPARATUS AND METHODS FOR HIGH THROUGHPUT BIOMOLECULE SEPARATION AND ANALYSIS 审中-公开用于高通量生物分子分离和分析的装置和方法公开(公告)号：US20120043208A1
公开(公告)日：2012-02-23
申请号：US13176704
申请日：2011-07-05
申请人： Jian Jin , Mark D. Biggin , Robert A. Nordmeyer , Ming Dong , Earl W. Cornell , Megan Choi , Halina Ewa Witkowski , Bong-Gyoon Han , Robert M. Glaeser
发明人： Jian Jin , Mark D. Biggin , Robert A. Nordmeyer , Ming Dong , Earl W. Cornell , Megan Choi , Halina Ewa Witkowski , Bong-Gyoon Han , Robert M. Glaeser
IPC分类号： G01N33/559 , C25B7/00 , G01N27/447
CPC分类号： G01N27/44756 , G01N27/44769
摘要： A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.
摘要翻译： 一种用于有效收集通过凝胶电泳分离的分子的多通道凝胶电泳装置，因此可以进一步分析，鉴定或用作试剂或药物。 该装置使用将内源性复合物的多维分离与其组成的质谱监测相结合的新型“无标记”策略。 在该方法中，基于通过多个正交分离步骤的多肽集合的共同迁移来鉴定推定的蛋白质复合物。 显示大部分大肠杆菌蛋白质通过三个色谱步骤在粗提取物分馏期间保持在稳定的络合物中。
-
-
-
-
-
-
-
-
-
搜索结果
10 条记录 (耗时 0.05 秒)
