公开(公告)号：US06929239B1

公开(公告)日：2005-08-16

申请号：US09786617

申请日：1999-09-08

申请人： Bruno Colin ,  Cécile Paris ,  Jacques Dachaud ,  Pierre Imbaud

发明人： Bruno Colin ,  Cécile Paris ,  Jacques Dachaud ,  Pierre Imbaud

IPC分类号： G01N1/00 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/68 ,  F15C3/00 ,  F16K1/14 ,  F16K1/16 ,  F16K11/00

CPC分类号： F16K99/0001 ,  B01L3/502738 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0605 ,  B01L2400/0638 ,  F16K99/0015 ,  F16K99/0023 ,  F16K2099/0071

摘要： The invention concerns a device for performing a reaction or at least two reactions in parallel or in series, and for transferring a fluid flow, consisting of. an upper surface (2) and a lower surface (3) mutually linked by an edge (4); at least a duct (5) wherein a fluid flow (F1) can be generated by means transferring at least one sample (6) to be treated and/or analysed, and at least a valve (8) incorporated in each duct (4) for directing each sample (6) thereby controlling the transfers, reactions and analyses in the device. Each valve (8) consists of two means (9 and 10) integral with the device, the first means (9) being mobile between an inoperative position wherein the fluid transfer (6) is possible and an active position wherein said transfer cannot take place, and the second means (10) being mobile between a position wherein it is not in contact with said first means (9) and an active position wherein it deforms the first means (9) and it is activated by an actuator external to the device.

摘要翻译： 本发明涉及一种用于并行或串联地进行反应或至少两个反应的装置，并用于传送由以下组成的流体流。 由边缘（4）相互连接的上表面（2）和下表面（3）; 至少一个管道（5），其中可以通过传送至少一个待处理和/或分析的样品（6）的装置产生流体流（F 1），以及至少一个结合在每个管道（4）中的阀门 ），用于引导每个样品（6），从而控制装置中的转移，反应和分析。 每个阀（8）由与装置成一体的两个装置（9和10）组成，第一装置（9）可移动在不可操作的位置，其中流体传送（6）是可能的，并且其中所述传送不能发生的活动位置 ，并且所述第二装置（10）在其不与所述第一装置（9）接触的位置和其中使所述第一装置（9）变形并且由所述装置外部的致动器启动的活动位置之间可移动 。

公开(公告)号：US07491497B2

公开(公告)日：2009-02-17

申请号：US11251758

申请日：2005-10-18

申请人： Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cécile Paris

发明人： Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cécile Paris

IPC分类号： C12Q1/68 ,  G01N21/00

CPC分类号： G01N35/1097 ,  B01L3/5025

摘要： A device for implementing a test card inside which sequential reactions and fluid transfer operations are performed under the effect of a control integrated into the card; the card includes at least two sequential reaction systems arranged in parallel, each reaction system having at least two fluid transfer systems arranged in series. A test card and the method for implementing the device and the card are also disclosed. The device contains at least one actuator per reaction line, and all the actuators are at a regular distance from one another, with the actuators preferably being equidistant. The invention is particularly applicable for the micromanipulation of fluids in biological applications.

摘要翻译： 一种用于实现测试卡的装置，其中在集成到卡中的控制的作用下执行顺序反应和流体转移操作; 卡片包括平行布置的至少两个顺序反应系统，每个反应系统具有串联布置的至少两个流体转移系统。 还公开了一种测试卡和用于实现设备和卡的方法。 该装置每个反应管线包含至少一个致动器，并且所有致动器彼此间隔一定距离，致动器优选等距。 本发明特别适用于生物应用中流体的显微操作。

公开(公告)号：US06410275B1

公开(公告)日：2002-06-25

申请号：US09420139

申请日：1999-10-18

申请人： Bryan Kluttz ,  Lawrence Burg ,  Garry Tegeler ,  Louis Graziano ,  Christopher Cotter ,  Michel Guy ,  James Greer ,  Geoff McKinley ,  Luigi Catanzariti ,  Robert Glassfold ,  James Clement Bishop ,  Bruno Colin ,  Cécile Paris ,  Thomas Wang ,  Michael Morin

发明人： Bryan Kluttz ,  Lawrence Burg ,  Garry Tegeler ,  Louis Graziano ,  Christopher Cotter ,  Michel Guy ,  James Greer ,  Geoff McKinley ,  Luigi Catanzariti ,  Robert Glassfold ,  James Clement Bishop ,  Bruno Colin ,  Cécile Paris ,  Thomas Wang ,  Michael Morin

IPC分类号： C12P1934

CPC分类号： B01L7/52 ,  B01L3/502 ,  B01L2200/026 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2400/049 ,  B01L2400/0644 ,  B01L2400/0655 ,  G01N2035/00366

摘要： A unit dose test device for a nucleic acid amplification reaction has the form of a elongate disposable test strip. The test strip includes a dual-chamber reaction vessel pre-loaded with nucleic acid amplification reaction reagents, and a plurality of wells for processing a reaction occurring in the reaction vessel.

摘要翻译： 用于核酸扩增反应的单位剂量测试装置具有细长的一次性测试条的形式。 测试条包括预装有核酸扩增反应试剂的双室反应容器和用于处理在反应容器中发生的反应的多个孔。

公开(公告)号：US20130109047A1

公开(公告)日：2013-05-02

申请号：US13808501

申请日：2011-07-08

申请人： Jean-Philippe Charrier ,  Bruno Colin ,  Laurent Drazek ,  Cécile Paris ,  Jean-Claude Raymond ,  Dominique Decaux

发明人： Jean-Philippe Charrier ,  Bruno Colin ,  Laurent Drazek ,  Cécile Paris ,  Jean-Claude Raymond ,  Dominique Decaux

IPC分类号： C12Q1/24

CPC分类号： C12Q1/24 ,  C12M33/04

摘要： The aim of the present invention is a method of sampling all or part of a sample (11) of biological matter (7), which is crude, enriched or cultured through contact with a culture medium (8), such as agar, for example in a Petri dish, using a probe (3) equipped with a terminal end (4), said sampling method comprising the steps of cooling the terminal end (4) of the probe (3), sticking all or part of the sample (11) of biological matter (7) to be sampled through contact, or by applying a pressure exerted by the terminal end (4) onto the sample (11) of biological matter (7), and sampling of all or part of the sample (11) of biological matter (7) so as to separate the sample (11) of biological matter (7) from the culture medium (8), a method of depositing into a container (9) or onto an analysis plate (14) all or part of a sample (11) of biological matter (7) stuck onto a frosted terminal end (4) of a probe, said depositing method including a step of separating the terminal end (4) of the probe (3) from all or part of the sample (11) of biological matter (7), as well as a device (2), a kit and an apparatus (1) implementing these methods.

摘要翻译： 本发明的目的是对生物物质（7）的全部或部分样品（11）进行取样的方法，其通过与诸如琼脂的培养基（8）接触而粗制，富集或培养。 在陪替氏培养皿中，使用配备有终端（4）的探针（3），所述取样方法包括冷却探针（3）的末端（4）的步骤，将全部或部分样品（11 ），或通过将终端（4）施加的压力施加到生物物质（7）的样品（11）上，并对样品（11）的全部或部分进行采样 ）将生物物质（7）的样品（11）与培养基（8）分离，将沉积到容器（9）或分析板（14）中的全部或者 将生物物质（7）的样品（11）的一部分粘贴到探针的磨砂末端（4）上，所述沉积方法包括将所述末端（4） 来自生物物质（7）的全部或部分样品（11）的探针（3）以及实施这些方法的装置（2），试剂盒和装置（1）。

公开(公告)号：US06660472B1

公开(公告)日：2003-12-09

申请号：US09509006

申请日：2000-07-17

申请人： Lise Santoro ,  Sophie Lopez ,  Bruno Colin ,  Corinne Jay ,  Cécile Paris ,  Kathleen Ann Clark Dickey

发明人： Lise Santoro ,  Sophie Lopez ,  Bruno Colin ,  Corinne Jay ,  Cécile Paris ,  Kathleen Ann Clark Dickey

IPC分类号： C12Q168

CPC分类号： C12N1/066 ,  Y10T436/143333

摘要： The invention concerns a lysis method for a biological sample comprising at least one micro-organism of the bacteria type, for releasing a nucleic material of interest belonging to said micro-organism, which consists in: providing in a container a biological sample in liquid medium; providing in said container at least one particulate material, relatively hard, and substantially inert with respect to the nucleic material; submitting the biological sample and particulate material mixture to a movement. The invention is characterised in that in combination, the selected movement is of the vortex type, and satisfies the following conditions: the particulate material consists of beads with diameter between 90 and 150 &mgr;m; and the apparent volume of the beads (Vb) and the volume of the liquid sample (Ve) are linked by the relationship Ve=&agr;. Vb, with &agr; ranging between 1.4 and 10 when the container is tubular in shape, and &agr; is not more than 2.1 when the container is in the shape of a disk; under such conditions, without any addition of reagent and/or additional process, the method consists in releasing directly into the liquid medium the nucleic material in native state and accessible to any reagent in a subsequent process.

摘要翻译： 本发明涉及包含至少一种细菌类微生物的生物样品的裂解方法，用于释放属于所述微生物的感兴趣的核酸，其包括：在容器中提供液体培养基中的生物样品 ; 在所述容器中提供相对于所述核材料相对较硬且基本上惰性的至少一种颗粒材料; 将生物样品和颗粒物质混合物提交到运动中。 本发明的特征在于，所选择的运动是涡流型的，并且满足以下条件：颗粒材料由直径在90至150μm之间的珠粒组成; 并且珠子（Vb）的表观体积和液体样品（Ve）的体积通过关系Ve =α连接。 Vb，当容器为管状形状时，α范围在1.4和10之间，当容器为盘形时，α不大于2.1; 在这种条件下，没有任何添加试剂和/或另外的方法，该方法包括将天然状态的核酸材料直接释放到液体介质中，并在随后的方法中可用于任何试剂。

公开(公告)号：US09650662B2

公开(公告)日：2017-05-16

申请号：US13808501

申请日：2011-07-08

申请人： Jean-Philippe Charrier ,  Bruno Colin ,  Laurent Drazek ,  Cécile Paris ,  Jean-Claude Raymond ,  Dominique Decaux

发明人： Jean-Philippe Charrier ,  Bruno Colin ,  Laurent Drazek ,  Cécile Paris ,  Jean-Claude Raymond ,  Dominique Decaux

IPC分类号： C12M1/26 ,  C12Q1/24

CPC分类号： C12Q1/24 ,  C12M33/04

摘要： The aim of the present invention is a method of sampling all or part of a sample (11) of biological matter (7), which is crude, enriched or cultured through contact with a culture medium (8), such as agar, for example in a Petri dish, using a probe (3) equipped with a terminal end (4), said sampling method comprising the steps of cooling the terminal end (4) of the probe (3), sticking all or part of the sample (11) of biological matter (7) to be sampled through contact, or by applying a pressure exerted by the terminal end (4) onto the sample (11) of biological matter (7), and sampling of all or part of the sample (11) of biological matter (7) so as to separate the sample (11) of biological matter (7) from the culture medium (8), a method of depositing into a container (9) or onto an analysis plate (14) all or part of a sample (11) of biological matter (7) stuck onto a frosted terminal end (4) of a probe, said depositing method including a step of separating the terminal end (4) of the probe (3) from all or part of the sample (11) of biological matter (7), as well as a device (2), a kit and an apparatus (1) implementing these methods.

公开(公告)号：US06632662B1

公开(公告)日：2003-10-14

申请号：US09743723

申请日：2001-03-02

申请人： Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

发明人： Patrick Broyer ,  Philippe Cleuziat ,  Bruno Colin ,  Cécile Paris ,  Lyse Santoro

IPC分类号： C12M133

CPC分类号： C12N1/066 ,  C12M47/06

摘要： The invention concerns a device for lysis (1) of micro-organism to release at least one intracellular biological constituent, comprising a container (2) wherein are present a biological sample, in liquid medium, containing the micro-organism to be lyzed, and a material in the form of particles, relatively hard and inert with respect to the sample. The invention also concerns a grinding method. The material in the form of particles comprises at least two types of grinding means into different dimensions: at least large dimension magnetic means (3) automatically controlled by a magnetic field; and at least small dimension means (4) actuated by the large dimension grinding means (3). The invention is useful for separating biological constituents.

摘要翻译： 本发明涉及用于释放至少一种细胞内生物成分的微生物的裂解装置（ 1 ），其包含容器（ 2 ），其中存在生物样品，在含有要溶解的微生物的液体培养基中，以及相对于样品相对较硬且惰性的颗粒形式的材料。 本发明还涉及研磨方法。 颗粒形式的材料包括至少两种不同尺寸的研磨装置：由磁场自动控制的至少大尺寸磁性装置（ 3 ）; 以及由大尺寸研磨装置（ 3 ）致动的至少小尺寸装置（ 4 ）。 本发明可用于分离生物成分

公开(公告)号：US09353398B2

公开(公告)日：2016-05-31

申请号：US13879887

申请日：2011-10-19

申请人： Bruno Colin ,  Cécile Paris

发明人： Bruno Colin ,  Cécile Paris

IPC分类号： C12Q1/24 ,  B01L3/00

CPC分类号： B01L3/50853 ,  B01L3/502738 ,  B01L2200/0689 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  C12Q1/24

摘要： The invention relates to a method for isolating at least one fluid sample well laid out in a test card for analysis, said test card including a plate (3) having at least one first main face from which is laid out at least one channel communicating with at least said well, this first face being coated with a membrane (17) provided with an adhesive (18) and which will cover said channel with a covering area (171), the method for isolation including a phase for introducing a fluid sample into at least said well and a phase for isolating said well consisting of exerting a force on at least one portion of the covering area (171) of the adhesive membrane (17) for ensuring displacement of the adhesive into an obturation area for the sample well.

摘要翻译： 本发明涉及一种隔离在测试卡中布置的至少一个流体样本的方法，用于分析，所述测试卡包括板（3），板（3）具有至少一个第一主面，布置有至少一个与 至少所述井，该第一面涂覆有具有粘合剂（18）的膜（17），并且其将覆盖所述通道并具有覆盖区域（171），所述隔离方法包括用于将流体样品引入的相 至少所述井以及用于隔离所述井的相，其包括在所述粘合膜（17）的覆盖区域（171）的至少一部分上施加力，以确保所述粘合剂移动到所述样品井的封闭区域。

公开(公告)号：US20140093428A1

公开(公告)日：2014-04-03

申请号：US14123145

申请日：2012-06-04

申请人： Hélène Briand ,  Bruno Colin ,  Cécile Paris

发明人： Hélène Briand ,  Bruno Colin ,  Cécile Paris

IPC分类号： G01N33/53 ,  B29C43/46

CPC分类号： G01N33/5302 ,  B29C43/46 ,  G01N33/54386 ,  G01N33/558 ,  G01N33/56988

摘要： The present invention relates to a device for performing an immunoassay, the device including a) a support, b) a porous matrix, fixed on the support, the matrix including (i) a liquid sample application area, (ii) a labeling area and (iii) at least one reaction area which includes a test results display area and a verification area, said liquid sample application area labeling area and reaction area being in fluid communication; the device including, at the verification area, at least a part of the porous matrix has pores with dimensions smaller than those of the labeled particles, such that at least a proportion of the labeled particles from the labeling area is stopped at said verification area forming a symbol which is visible or displayable for a user; and a method for manufacturing the device.

摘要翻译： 本发明涉及一种用于进行免疫测定的装置，该装置包括a）支撑体，b）固定在载体上的多孔基质，所述基质包括（i）液体样品施用区域，（ii）标记区域和 （iii）至少一个包括测试结果显示区域和验证区域的反应区域，所述液体样品应用区域标记区域和反应区域处于流体连通状态; 所述装置包括在验证区域处的多孔基质的至少一部分具有尺寸小于被标记颗粒尺寸的孔，使得至少一部分来自标记区域的标记颗粒在所述验证区域形成 对用户可见或可显示的符号; 以及该装置的制造方法。

公开(公告)号：US07537730B2

公开(公告)日：2009-05-26

申请号：US10362873

申请日：2001-08-27

申请人： Bruno Colin ,  Cécile Paris ,  Bernard Limon ,  Patrick Broyer ,  Charles Rogers

发明人： Bruno Colin ,  Cécile Paris ,  Bernard Limon ,  Patrick Broyer ,  Charles Rogers

IPC分类号： F16K35/00 ,  B01L11/00 ,  G01N21/00 ,  F17D1/16

CPC分类号： B01L3/502738 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0638 ,  B01L2400/0655 ,  G01N2035/00247 ,  G01N2035/00257 ,  Y10T137/0396

摘要： A reaction card (1) having a body, a front surface (62) and a rear surface (63) defined by an edge (68), at least one inlet (2, 3, 4 and/or 5) and at least one outlet (7and/or 8), connected by a network of channels (64) constituting at least one reaction path for at least one fluid which is directed via valves (11to 36 and/or 61); each valve includes a flexible film (67) which can be deformed to allow fluid passage, the film being fixed on the card's rear surface at a peripheral indentation of an assembly of channels associated with the valve; the card includes channels flush with at least one of its surfaces, the channels being of two different cross-sections, a small cross-section serving as a reaction compartment; each front or rear surface is delimited by at least one film (48, 49, 65, 66 and/or 67).

摘要翻译： 具有主体的反应卡（1），由边缘（68）限定的前表面（62）和后表面（63），至少一个入口（2,3,4和/或5）和至少一个 出口（7和/或8）通过构成用于至少一个通过阀（11至36和/或61）的流体的至少一个反应路径的通道网络（64）连接; 每个阀包括柔性膜（67），其可以变形以允许流体通过，所述膜被固定在卡的后表面上与阀相关联的通道组件的周边凹陷处; 卡包括与其表面中的至少一个平齐的通道，通道具有两个不同的横截面，小的横截面用作反应室; 每个前表面或后表面由至少一个膜（48,49,65,66和/或67）限定。