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14 条记录 (耗时 0.023 秒)

    Modified yeasts and uses thereof, in particular for producing steroid derivatives
    1.
    发明授权
    Modified yeasts and uses thereof, in particular for producing steroid derivatives 有权
    改良的酵母及其用途，特别是用于生产类固醇衍生物

    公开(公告)号：US07879592B2

    公开(公告)日：2011-02-01

    申请号：US10343993

    申请日：2001-07-24

    申请人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    发明人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    IPC分类号： C12N1/14 ,  C12N1/20 ,  C12N9/04 ,  C12N15/00 ,  C12Q1/68 ,  C07H21/04 ,  C07H21/02 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/26 ,  C12N9/00

    CPC分类号： C12N9/0087 ,  C12P33/06

    摘要： The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20αHSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.

    摘要翻译： 本发明涉及新型酵母菌株，其制备方法和遗传构建体及其用于合成或修饰甾族化合物的用途。 更具体地，本发明描述了具有降低的20αHSD型活性的菌株，特别是通过修饰GCY1和/或YPR1基因。 本发明的酵母菌株可以提高合成的效率或增加方法的选择性或产率以及最终产品的质量。 本发明的菌株，方法和化合物可用于寻找，开发和生产具有治疗或预防活性的人或动物，特别是甾族衍生物的产品。

    Modified yeasts and uses thereof, in particular for producing steroid derivatives
    4.
    发明授权
    Modified yeasts and uses thereof, in particular for producing steroid derivatives 有权
    改良的酵母及其用途，特别是用于生产类固醇衍生物

    公开(公告)号：US08173402B2

    公开(公告)日：2012-05-08

    申请号：US13006607

    申请日：2011-01-14

    申请人： Bruno Dumas ,  Gilles Cauet ,  Eric Degryse ,  Tilman Achstetter

    发明人： Bruno Dumas ,  Gilles Cauet ,  Eric Degryse ,  Tilman Achstetter

    IPC分类号： C12P7/00 ,  C12N15/00 ,  C12N1/20 ,  C12N1/14 ,  C12N9/00 ,  C12N9/04 ,  C12Q1/34 ,  C12Q1/26 ,  C07H21/04 ,  C07H21/02

    CPC分类号： C12N9/0087 ,  C12P33/06

    摘要： The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20αHSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.

    摘要翻译： 本发明涉及新型酵母菌株，其制备方法和遗传构建体及其用于合成或修饰甾族化合物的用途。 更具体地，本发明描述了具有降低的20αHSD型活性的菌株，特别是通过修饰GCY1和/或YPR1基因。 本发明的酵母菌株可以提高合成的效率或提高方法的选择性或产率以及最终产品的质量。 本发明的菌株，方法和化合物可用于寻找，开发和生产具有治疗或预防活性的人或动物，特别是甾类衍生物的产品。

    MODIFIED YEASTS AND USES THEREOF, IN PARTICULAR FOR PRODUCING STEROID DERIVATIVES
    5.
    发明申请
    MODIFIED YEASTS AND USES THEREOF, IN PARTICULAR FOR PRODUCING STEROID DERIVATIVES 有权
    改良的YEASTS及其用途，特别是生产甾类衍生物

    公开(公告)号：US20110104749A1

    公开(公告)日：2011-05-05

    申请号：US13006607

    申请日：2011-01-14

    申请人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    发明人： Bruno Dumas ,  Gilles Cauet ,  Tilman Achstetter ,  Eric Degryse

    IPC分类号： C12P33/00

    CPC分类号： C12N9/0087 ,  C12P33/06

    摘要： The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20αHSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.

    摘要翻译： 本发明涉及新型酵母菌株，其制备方法和遗传构建体及其用于合成或修饰甾族化合物的用途。 更具体地，本发明描述了具有降低的20αHSD型活性的菌株，特别是通过修饰GCY1和/或YPR1基因。 本发明的酵母菌株可以提高合成的效率或提高方法的选择性或产率以及最终产品的质量。 本发明的菌株，方法和化合物可用于寻找，开发和生产具有治疗或预防活性的人或动物，特别是甾类衍生物的产品。

    Yeast strains for the production of mature heterologous proteins, especially hirudin
    8.
    发明授权
    Yeast strains for the production of mature heterologous proteins, especially hirudin 失效
    用于生产成熟异源蛋白质的酵母菌株，特别是水蛭素

    公开(公告)号：US5879926A

    公开(公告)日：1999-03-09

    申请号：US485515

    申请日：1995-06-07

    申请人： Yves Lemoine ,  Martine Nguyen ,  Tilman Achstetter

    发明人： Yves Lemoine ,  Martine Nguyen ,  Tilman Achstetter

    IPC分类号： C07K14/815 ,  C12N1/19 ,  C12N9/48 ,  C12N15/67 ,  C12N15/81 ,  C07H21/04 ,  C12N15/12 ,  C12N15/15

    CPC分类号： C12R1/865 ,  C07K14/815 ,  C12N15/67 ,  C12N15/81 ,  C12N9/48 ,  C07K2319/02

    摘要： The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.

    摘要翻译： 本发明涉及通过包含含有编码异源蛋白质的序列的表达载体的酵母产生成熟异源蛋白质，特别是水蛭素的改进体系。 该改进的特征在于扩增酵母的KEX2基因，编码内切蛋白酶yscF。 通过将酵母基因组中全部或部分全部或部分KEX2基因的一个或多个拷贝整合进入扩增，或者通过将全部或部分全部或部分KEX2基因的一个或多个拷贝插入载体中来表达异源 蛋白。

    Promoters for expressing proteins of interest in yeast
    9.
    发明授权
    Promoters for expressing proteins of interest in yeast 失效
    在酵母中表达目的蛋白的启动子

    公开(公告)号：US5952195A

    公开(公告)日：1999-09-14

    申请号：US930617

    申请日：1998-03-09

    申请人： Valerie Nacken ,  Tilman Achstetter

    发明人： Valerie Nacken ,  Tilman Achstetter

    IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N15/81 ,  C12P21/02 ,  C12R1/865 ,  C07H21/04 ,  C12N15/63

    CPC分类号： C12N15/81

    摘要： The present invention provides novel nucleic acid fragments isolated from the genomic DNA of yeast and having transcription promoter activity, as well as expression cassettes, vectors, and host cells containing said nucleic acid fragments. The present invention also provides methods for incorporating the nucleic acid fragments of the present invention for producing commercially or therapeutically useful polypeptides.

    摘要翻译： PCT No.PCT / FR96 / 00523 Sec。 371日期1998年3月9日 102（e）1998年3月9日PCT 1996年4月5日PCT PCT。 公开号WO96 / 31611 日本1996年10月10日本发明提供从酵母的基因组DNA分离并具有转录启动子活性的新型核酸片段，以及含有所述核酸片段的表达盒，载体和宿主细胞。 本发明还提供了用于掺入本发明的核酸片段用于生产商业上或治疗上有用的多肽的方法。