公开(公告)号：US08465942B2

公开(公告)日：2013-06-18

申请号：US13527333

申请日：2012-06-19

申请人： Christopher L. G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

发明人： Christopher L. G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  C12N9/14 ,  C12N1/20 ,  C12N15/00 ,  C12P7/00 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12P7/6454 ,  C12N9/14 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。

公开(公告)号：US08357503B2

公开(公告)日：2013-01-22

申请号：US12550300

申请日：2009-08-28

申请人： Christopher L. G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

发明人： Christopher L. G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  C12N9/14 ,  C12N1/20 ,  C12N15/00 ,  C12P7/00 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12P7/6454 ,  C12N9/14 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

公开(公告)号：US20100129882A1

公开(公告)日：2010-05-27

申请号：US12550300

申请日：2009-08-28

申请人： Christopher L.G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

发明人： Christopher L.G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

IPC分类号： C12P7/64

CPC分类号： C12P7/6454 ,  C12N9/14 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

公开(公告)号：US20120258505A1

公开(公告)日：2012-10-11

申请号：US13527333

申请日：2012-06-19

申请人： Christopher L.G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

发明人： Christopher L.G. Dayton ,  Nelson Barton ,  Analia Bueno ,  Joslin G. Cuenca ,  Tim Hitchman ,  Katie A. Kline ,  Jonathan Lyon ,  Mark L. Miller ,  Mark A. Wall

IPC分类号： C12P7/64

CPC分类号： C12P7/6454 ,  C12N9/14 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。

公开(公告)号：US09238804B2

公开(公告)日：2016-01-19

申请号：US13471206

申请日：2012-05-14

申请人： Tim Hitchman ,  Christopher L. G. Dayton ,  Katie A. Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

发明人： Tim Hitchman ,  Christopher L. G. Dayton ,  Katie A. Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

IPC分类号： C12N9/20 ,  C12N9/16

CPC分类号： C12N9/20 ,  A23L29/06 ,  A23V2002/00 ,  A61K8/64 ,  A61K8/66 ,  A61K2800/10 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/16 ,  C12Y301/01021

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

公开(公告)号：US20100055085A1

公开(公告)日：2010-03-04

申请号：US12202119

申请日：2008-08-29

申请人： Tim HITCHMAN ,  Christopher L. G. Dayton ,  Katie A. Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

发明人： Tim HITCHMAN ,  Christopher L. G. Dayton ,  Katie A. Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

IPC分类号： C12N9/14 ,  C12N15/11 ,  C12N15/13 ,  C12N15/52 ,  C12N15/63 ,  C12N1/20 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  A01H5/00 ,  A01K67/033 ,  C07K14/00 ,  C12N9/20 ,  C12N9/16 ,  C12N11/02 ,  C12N11/14 ,  C12N11/08 ,  C07K16/00 ,  C12P21/06 ,  C12N9/96 ,  C11D3/386 ,  C12P7/24 ,  A61K38/46 ,  C07H21/00 ,  A23L1/30

CPC分类号： C12N9/20 ,  A23L29/06 ,  A23V2002/00 ,  A61K8/64 ,  A61K8/66 ,  A61K2800/10 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/16 ,  C12Y301/01021

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

公开(公告)号：US09045712B2

公开(公告)日：2015-06-02

申请号：US13502105

申请日：2010-10-08

申请人： Christopher L. G. Dayton ,  Flavio Da Silva Galhardo ,  Nelson Barton ,  Tim Hitchman ,  Jonathan Lyon ,  Eileen O'Donoghue ,  Mark A. Wall

发明人： Christopher L. G. Dayton ,  Flavio Da Silva Galhardo ,  Nelson Barton ,  Tim Hitchman ,  Jonathan Lyon ,  Eileen O'Donoghue ,  Mark A. Wall

IPC分类号： C11B3/04 ,  C12P7/18 ,  C12N9/88 ,  C11B3/00 ,  C10L1/02 ,  C12P7/64

CPC分类号： C11B3/003 ,  C10G2300/1011 ,  C10L1/026 ,  C11B3/04 ,  C12N9/88 ,  C12P7/18 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y406/01013 ,  Y02E50/13 ,  Y02P20/52 ,  Y02P30/20

摘要： In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.

摘要翻译： 在替代实施方案中，本发明提供磷脂酰肌醇特异性磷脂酶C（PI-PLC）酶，编码它们的核酸，与其特异性结合的抗体，以及制备和使用它们的方法。 还提供了包括使用这些磷脂酶的工业方法和产品。 在某些实施方案中，本文提供了用于在脂质基质内水合非水合磷脂（NHP）的方法。 这些方法使NHP迁移到油 - 水界面，从而允许NHP从脂质中反应和/或除去。 在某些实施方案中，提供了从植物油中除去NHP，可水合磷脂和卵磷脂以产生可用于食品生产和/或非食品应用的脱胶油或脂肪产品的方法。 在某些实施方案中，本文提供了用于NHP水合的方法，然后进行酶处理和除去各种磷脂和卵磷脂。 本文提供的方法可以在粗脱水油或水脱胶油上实施。

公开(公告)号：US08313918B2

公开(公告)日：2012-11-20

申请号：US13083461

申请日：2011-04-08

申请人： Christopher L. G. Dayton ,  Tim Hitchman ,  Katie Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

发明人： Christopher L. G. Dayton ,  Tim Hitchman ,  Katie Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  C12N9/14 ,  C12N1/20 ,  C12N15/00 ,  C12P7/00 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12P7/6472 ,  A23D7/04 ,  A23D9/04 ,  C11B3/003 ,  C11C1/045 ,  C11C3/08 ,  C11C3/12 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6427 ,  C12P7/6445 ,  C12P7/6454

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。

公开(公告)号：US08349578B2

公开(公告)日：2013-01-08

申请号：US13083484

申请日：2011-04-08

申请人： Christopher L. G. Dayton ,  Tim Hitchman ,  Katie Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

发明人： Christopher L. G. Dayton ,  Tim Hitchman ,  Katie Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  C12N9/14 ,  C12N1/20 ,  C12N15/00 ,  C12P7/00 ,  C12P21/06 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12P7/6472 ,  A23D7/04 ,  A23D9/04 ,  C11B3/003 ,  C11C1/045 ,  C11C3/08 ,  C11C3/12 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6427 ,  C12P7/6445 ,  C12P7/6454

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

摘要翻译： 提供水解酶，包括脂肪酶，饱和酶，棕榈酶和/或硬脂酸酶，以及编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 进一步提供的是具有水解酶活性的多肽，例如脂肪酶，饱和酶，棕榈酸酶和/或硬脂酸酶，以及用于制备低饱和脂肪酸或低反式脂肪油如低饱和度或低反式脂肪动物或植物油的方法， 例如，大豆油或卡诺拉油。

公开(公告)号：US08541191B2

公开(公告)日：2013-09-24

申请号：US13083480

申请日：2011-04-08

申请人： Christopher L. G. Dayton ,  Tim Hitchman ,  Katie Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

发明人： Christopher L. G. Dayton ,  Tim Hitchman ,  Katie Kline ,  Jonathan Lyon ,  Mark A. Wall ,  Nelson R. Barton

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  C21N9/14 ,  C21N1/20 ,  C21N15/00 ,  C12P7/00 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12P7/6472 ,  A23D7/04 ,  A23D9/04 ,  C11B3/003 ,  C11C1/045 ,  C11C3/08 ,  C11C3/12 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6427 ,  C12P7/6445 ,  C12P7/6454

摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.