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    O-phosphoserine sulfhydrylase mutants and method for production of cysteine using the same
    6.
    发明授权
    O-phosphoserine sulfhydrylase mutants and method for production of cysteine using the same 有权
    O-磷酸丝氨酸巯基酸酶突变体及其制备方法

    公开(公告)号：US09127324B2

    公开(公告)日：2015-09-08

    申请号：US13278100

    申请日：2011-10-20

    申请人： Soo An Shin ,  Jin Sook Chang ,  Hye Won Um ,  Jae Hyun Jo ,  Byeong Cheol Song ,  Kyoung Min Lee

    发明人： Soo An Shin ,  Jin Sook Chang ,  Hye Won Um ,  Jae Hyun Jo ,  Byeong Cheol Song ,  Kyoung Min Lee

    IPC分类号： C12N9/10 ,  C12P13/12

    CPC分类号： C12Y205/01065 ,  C12N9/1085 ,  C12P13/12

    摘要： Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a Mycobacterium smegmatis-derived amino acid sequence corresponding to that of SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. Also, a nucleic acid molecule encoding the OPSS mutant, an expression vector carrying the nucleic acid molecule, and a transformant transformed with the expression vector are disclosed. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulfide in the presence of the OPSS mutant. The OPSS mutant has improved enzymatic activity and can be applied to the environmentally friendly production of L-cysteine through a simple enzymatic conversion reaction.

    摘要翻译： 公开了一种O-磷酸丝氨酸二硫氢化酶（OPSS）突变体，其具有对应于SEQ ID NO：1的不含3至7个C末端氨基酸残基的分枝杆菌来源的氨基酸序列。 此外，公开了编码OPSS突变体的核酸分子，携带核酸分子的表达载体和用表达载体转化的转化体。 此外，提供了在OPSS突变体的存在下，使O-磷酸-L-丝氨酸（OPS）与硫化物反应的半胱氨酸的制造方法。 OPSS突变体具有改善的酶活性，可以通过简单的酶转化反应应用于环保生产L-半胱氨酸。