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105 条记录 (耗时 0.028 秒)

    Optimal procedure for isolation of mutant mitochondrial alleles
    1.
    发明授权
    Optimal procedure for isolation of mutant mitochondrial alleles 失效
    分离突变体线粒体等位基因的最佳方法

    公开(公告)号：US6027883A

    公开(公告)日：2000-02-22

    申请号：US757438

    申请日：1996-11-27

    申请人： Corinna Herrnstadt ,  Soumitra Ghosh ,  Eoin D. Fahy ,  Robert E. Davis

    发明人： Corinna Herrnstadt ,  Soumitra Ghosh ,  Eoin D. Fahy ,  Robert E. Davis

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16

    摘要： A sample preparation procedure for mitochondrial (mt) DNA analysis is described. The present method for isolating mtDNA uses sedimentation techniques for separating erythrocytes from lymphocytes and platelets (crude buffy coat fraction), followed by DNA extraction from the crude buffy coat fraction by boiling in water. This approach improves the yield of mutant DNA, enhancing the sensitivity of subsequent mutation interrogation techniques and allowing for meaningful statistical treatment of the degree of heteroplasmy within the mitochondrial DNA.

    摘要翻译： 描述了线粒体（mt）DNA分析的样品制备程序。 目前用于分离mtDNA的方法使用沉淀技术从淋巴细胞和血小板（粗血沉棕黄层级分离）中分离红细胞，然后通过在水中沸腾从粗血沉棕黄层级分提取DNA。 这种方法提高了突变DNA的产量，提高了随后的突变询问技术的敏感性，并允许有意义的统计学处理线粒体DNA内的异质性程度。

    Diagnostic assay for diabetes mellitus based on mutational burden
    5.
    发明授权
    Diagnostic assay for diabetes mellitus based on mutational burden 失效
    基于突变负担的糖尿病诊断测定

    公开(公告)号：US06291172B1

    公开(公告)日：2001-09-18

    申请号：US09302682

    申请日：1999-04-30

    申请人： Robert E. Davis ,  Corinna Herrnstadt

    发明人： Robert E. Davis ,  Corinna Herrnstadt

    IPC分类号： C12P1934

    CPC分类号： C07D219/08 ,  A61K38/00 ,  C07K14/4711 ,  C07K14/4713 ,  C12N9/0053 ,  C12Q1/6806 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2527/101

    摘要： The present invention relates to genetic mutations in mitochondrial genes that segregate with diabetes mellitus. The invention provides methods for detecting such mutations, as a diagnostic for diabetes mellitus, either before or after the onset of clinical symptoms. Examples of specific mutations in the ATP synthase 8/6 sequence and tRNALys sequence are given. The invention also provides treatments for dysfunctions due to genes for mitochondrial functions that segregate with diabetes mellitus. Cybrid cell lines are described which are useful as model systems for the study of the mitochondrial metabolic disorders that are associated with diabetes mellitus, and for identifying therapeutic compounds and treatments for this disease.

    摘要翻译： 本发明涉及与糖尿病分离的线粒体基因中的遗传突变。 本发明提供在临床症状发作之前或之后检测这种突变作为糖尿病诊断的方法。 给出了ATP合酶8/6序列和tRNALys序列中特异性突变的实例。 本发明还提供了由于与糖尿病分离的线粒体功能的基因引起的功能障碍的治疗。 描述了杂交细胞系，其可用作研究与糖尿病相关的线粒体代谢紊乱的模型系统，并且用于鉴定该疾病的治疗化合物和治疗。

    Compounds and methods for treating mitochondria-associated diseases
    9.
    发明授权
    Compounds and methods for treating mitochondria-associated diseases 失效
    用于治疗线粒体相关疾病的化合物和方法

    公开(公告)号：US06268398B1

    公开(公告)日：2001-07-31

    申请号：US09299044

    申请日：1999-04-23

    申请人： Soumitra Ghosh ,  Robert E. Davis

    发明人： Soumitra Ghosh ,  Robert E. Davis

    IPC分类号： A61K31155

    CPC分类号： A61K31/5375 ,  A61K31/155 ,  A61K31/196

    摘要： Compounds, compositions and methods are disclosed for treating mitochondria-associated diseases, such as cancer, psoriasis, stroke, Alzheimer's Disease and diabetes. The compounds of this invention have structure (I) below, including stereoisomers, prodrugs and pharmaceutically acceptable salts thereof, wherein Ar and L are as defined herein. The methods of this invention are directed to treating a mitochondria-associated disease by administering to a warm-blooded animal in need thereof an effective amount of a compound of structure (I), typically in the form of a pharmaceutical composition.

    摘要翻译： 公开了用于治疗线粒体相关疾病如癌症，牛皮癣，中风，阿尔茨海默病和糖尿病的化合物，组合物和方法。 本发明的化合物具有以下结构（I），包括其立体异构体，前药和药学上可接受的盐，其中Ar和L如本文所定义。 本发明的方法涉及通过向有需要的温血动物施用有效量的通常为药物组合物形式的结构（I）的化合物来治疗线粒体相关疾病。

    Method and apparatus to use remote and local control modes to acquire and visually present data
    10.
    发明授权
    Method and apparatus to use remote and local control modes to acquire and visually present data 有权
    使用远程和本地控制模式来获取和可视化呈现数据的方法和装置

    公开(公告)号：US09117321B2

    公开(公告)日：2015-08-25

    申请号：US12913249

    申请日：2010-10-27

    申请人： Robert E. Davis ,  Michel B. Alberry ,  Timothy G. Ruther ,  Anthony J. Cichy

    发明人： Robert E. Davis ,  Michel B. Alberry ,  Timothy G. Ruther ,  Anthony J. Cichy

    IPC分类号： G06F3/00 ,  G01M17/00 ,  A61B5/00 ,  G07C5/08 ,  G07C5/00

    CPC分类号： G07C5/0825 ,  G07C5/008 ,  G07C2205/02

    摘要： A system comprising multiple devices that are operable when servicing a device-under service is described. A data acquisition (DAQ) device and a vehicle scanner device of the system are operable to acquire data from the device-under-service and to transmit the acquired data to a display device of the system. The DAQ device can operate in a local-control mode in which selection of DAQ mode for the DAQ device is carried out at the DAQ device. The DAQ device can operate in a remote-control mode in which selection of a DAQ mode for the DAQ device is carried out at the display device. The multiple devices may communicate with each other via one or more wireless network via one or more air interface protocols. Each device of the system may operate as a stand-alone device or in combination with multiple devices of the system.

    摘要翻译： 描述了包括在服务于正在服务的设备时可操作的多个设备的系统。 系统的数据采集（DAQ）设备和车辆扫描仪设备可操作以从服务中的设备获取数据并将所获取的数据发送到系统的显示设备。 DAQ设备可以在DAQ设备中执行DAQ设备的DAQ模式选择的本地控制模式下工作。 DAQ设备可以在远程控制模式下操作，其中在显示设备上执行DAQ设备的DAQ模式的选择。 多个设备可以经由一个或多个空中接口协议经由一个或多个无线网络彼此通信。 系统的每个设备可以作为独立设备操作，或者与系统的多个设备组合。