公开(公告)号：US20150285685A1

公开(公告)日：2015-10-08

申请号：US14683066

申请日：2015-04-09

Applicant: Duke University

Inventor： Adam Wax ,  Howard Levinson ,  William J. Brown ,  Thomas Matthews ,  Manuel Medina

IPC: G01J3/28 ,  G01J3/453 ,  G01N21/25 ,  G01B9/02

CPC classification number: G01J3/2823 ,  A61B5/445 ,  G01B9/02044 ,  G01B9/02091 ,  G01J3/0218 ,  G01J3/453 ,  G01J3/4531 ,  G01N21/45 ,  G01N21/4795

Abstract: Disclosed herein are systems and methods for deep spectroscopic imaging of a biological sample. In an aspect, a system includes a broad bandwidth light source configured to generate an illumination beam, an interferometer, and a spectrometer. The interferometer includes a first beam splitter configured to split the illumination beam into an incident beam and a reference beam; an optical lens directs the incident beam onto a biological sample at a predefined offset from corresponding optical axis, and receive a beam scattered from the biological sample. The beams are configured to intersect with each other within a focal zone of the optical lens. Photons of the incident beam undergo multiple forward scattering within the biological sample. A second beam splitter configured to receive and superimpose the scattered and reference beams, to generate an interference beam. The spectrometer uses a spectral domain detection technique to assess tissue properties of the biological sample.

Abstract translation: 本文公开了用于生物样品的深度光谱成像的系统和方法。 在一方面，系统包括配置成产生照明光束的宽带宽光源，干涉仪和光谱仪。 所述干涉仪包括：第一分束器，被配置为将照明光束分成入射光束和参考光束; 光学透镜将入射光束以预定偏移量从对应的光轴引导到生物样品上，并接收从生物样品散射的光束。 梁被构造成在光学透镜的聚焦区域内彼此相交。 入射光束的光子在生物样品内经历多次前向散射。 第二分束器，被配置为接收和叠加散射参考光束并产生干涉光束。 光谱仪使用光谱域检测技术来评估生物样品的组织性质。

公开(公告)号：US20150062591A1

公开(公告)日：2015-03-05

申请号：US14523174

申请日：2014-10-24

Applicant: Duke University

Inventor： Adam Wax ,  William J. Brown

IPC: G01B9/02

CPC classification number: G01B9/0209 ,  A61B5/0059 ,  G01B9/02004 ,  G01B9/02043 ,  G01B9/02084 ,  G01B9/02088 ,  G01N21/4795

Abstract: Embodiments described herein involve low-coherence interferometry (LCI) techniques which enable acquisition of structural and depth information regarding a sample of interest. In one embodiment, a “swept-source” (SS) light source is used in LCI to obtain structural and depth information about a sample. The swept-source light source can be used to generate a reference signal and a signal directed towards a sample. Light scattered from the sample is returned as a result and mixed with the reference signal to achieve interference and thus provide structural information regarding the sample. Depth information about the sample can be obtained using Fourier domain concepts as well as time domain techniques. Several LCI embodiments employing a swept-source light source are disclosed herein. In another embodiment disclosed herein, an a/LCI system and method is provided that is based on a time domain system and employs a broadband light source. The systems and processes disclosed herein can be used for biomedical applications, including measuring cellular morphology in tissues and in vitro as well as diagnosing intraepithelial neoplasia, and assessing the efficacy of chemopreventive and chemotherapeutic agents.

Abstract translation: 本文描述的实施例涉及低相干干涉测量（LCI）技术，其能够获取关于感兴趣的样本的结构和深度信息。 在一个实施例中，在LCI中使用“扫频源”（SS）光源以获得关于样本的结构和深度信息。 扫描光源可用于产生参考信号和指向样品的信号。 作为结果返回从样品散射的光，并与参考信号混合以实现干扰，从而提供关于样品的结构信息。 可以使用傅里叶域概念以及时域技术获得关于样本的深度信息。 本文公开了采用扫频源光源的几个LCI实施例。 在本文公开的另一实施例中，提供了基于时域系统并采用宽带光源的a / LCI系统和方法。 本文公开的系统和方法可用于生物医学应用，包括测量组织和体外的细胞形态，以及诊断上皮内瘤变，以及评估化学预防和化学治疗剂的功效。

公开(公告)号：US20150045677A1

公开(公告)日：2015-02-12

申请号：US14494080

申请日：2014-09-23

Applicant: Duke University

Inventor： Adam Wax ,  John W. Pyhtila

IPC: A61B5/00 ,  A61B1/00 ,  G01B9/02

CPC classification number: A61B5/0084 ,  A61B1/00165 ,  A61B1/00172 ,  A61B5/0066 ,  A61B5/0075 ,  G01B9/02043 ,  G01B9/02044 ,  G01B9/02084 ,  G01B9/02087 ,  G01B9/0209 ,  G01B9/02091 ,  G01J3/45 ,  G01N21/31 ,  G01N21/4795 ,  G01N2021/4704 ,  G01N2021/4709 ,  G01N2021/4735 ,  G01N2201/08

Abstract: A method of assessing tissue health comprises the steps of obtaining depth-resolved spectra of a selected area of in vivo tissue, and assessing the health of the selected area based on the depth-resolved structural information of the scatterers. Obtaining depth-resolved spectra of the selected area comprises directing a sample beam towards the selected area at an angle, and receiving an angle-resolved scattered sample beam. The angle-resolved scattered sample beam is cross-correlated with the reference beam to produce an angle-resolved cross-correlated signal about the selected area, which is spectrally dispersed to yield an angle-resolved, spectrally-resolved cross-correlation profile having depth-resolved information about the selected area. The angle-resolved, spectrally-resolved cross-correlation profile is processed to obtain depth-resolved information about scatterers in the selected area.

Abstract translation: 评估组织健康的方法包括以下步骤：获得体内组织的选定区域的深度分辨光谱，以及基于散射体的深度分辨结构信息来评估所选区域的健康状况。 获得所选区域的深度分辨光谱包括将样品光束以一定角度朝向选定区域引导，并且接收角度分辨的散射样品光束。 角度分辨的散射采样光束与参考光束交叉相关，以产生关于所选区域的角度分辨的交叉相关信号，其被频谱分散以产生具有深度的角度分辨，频谱分辨的互相关分布 解决有关所选区域的信息。 处理角度分辨的，光谱解析的互相关轮廓以获得关于所选区域中的散射体的深度解析信息。

公开(公告)号：US08860945B2

公开(公告)日：2014-10-14

申请号：US13868215

申请日：2013-04-23

Applicant: Duke University

Inventor： Adam Wax ,  John W. Pyhtila

IPC: G01B9/02 ,  G01J3/45 ,  G01N21/31 ,  A61B5/00 ,  G01N21/47

CPC classification number: A61B5/0084 ,  A61B1/00165 ,  A61B1/00172 ,  A61B5/0066 ,  A61B5/0075 ,  G01B9/02043 ,  G01B9/02044 ,  G01B9/02084 ,  G01B9/02087 ,  G01B9/0209 ,  G01B9/02091 ,  G01J3/45 ,  G01N21/31 ,  G01N21/4795 ,  G01N2021/4704 ,  G01N2021/4709 ,  G01N2021/4735 ,  G01N2201/08

Abstract: Fourier domain a/LCI (faLCI) system and method which enables in vivo data acquisition at rapid rates using a single scan. Angle-resolved and depth resolved spectra information is obtained with one scan. The reference arm can remain fixed with respect to the sample due to only one scan required. A reference signal and a reflected sample signal are cross-correlated and dispersed at a multitude of reflected angles off of the sample, thereby representing reflections from a multitude of points on the sample at the same time in parallel. Information about all depths of the sample at each of the multitude of different points on the sample can be obtained with one scan on the order of approximately 40 milliseconds. From the spatial, cross-correlated reference signal, structural (size) information can also be obtained using techniques that allow size information of scatterers to be obtained from angle-resolved data.

Abstract translation: 傅里叶域a / LCI（faLCI）系统和方法，使用单次扫描能够以快速的速率进行体内数据采集。 通过一次扫描获得角度分辨和深度解析的光谱信息。 由于仅需要一次扫描，参考臂可以相对于样品保持固定。 参考信号和反射的采样信号以相对于样本的多个反射角度相互相关并分散，从而同时并行地表示来自样品上的多个点的反射。 可以通过大约40毫秒量级的一次扫描获得关于样本上多个不同点的每个样本的所有深度的信息。 从空间相互关联的参考信号，也可以使用允许从角度分辨数据获得散射体的尺寸信息的技术来获得结构（尺寸）信息。

公开(公告)号：US20130176560A1

公开(公告)日：2013-07-11

申请号：US13657517

申请日：2012-10-22

Applicant: Duke University

Inventor： Adam Wax ,  Robert N. Graf ,  Francisco E. Robles

IPC: G01J3/45

CPC classification number: G01J3/453 ,  G01B9/02044 ,  G01B9/02084 ,  G01B9/02087 ,  G01B9/02091 ,  G01J3/45 ,  G01J2003/4538 ,  G01N21/49

Abstract: Current apparatuses and methods for analysis of spectroscopic optical coherence tomography (SOCT) signals suffer from an inherent tradeoff between time (depth) and frequency (wavelength) resolution. In one non-limiting embodiment, multiple or dual window (DW) apparatuses and methods for reconstructing time-frequency distributions (TFDs) that applies two windows that independently determine the optical and temporal resolution is provided. For example, optical resolution may relate to scattering information about a sample, and temporal resolution may be related to absorption or depth related information. The effectiveness of the apparatuses and methods is demonstrated in simulations and in processing of measured OCT signals that contain fields which vary in time and frequency. The DW technique may yield TFDs that maintain high spectral and temporal resolution and are free from the artifacts and limitations commonly observed with other processing methods.

公开(公告)号：US09823127B2

公开(公告)日：2017-11-21

申请号：US14683066

申请日：2015-04-09

Applicant: Duke University

Inventor： Adam Wax ,  Howard Levinson ,  William J. Brown ,  Thomas Matthews ,  Manuel Medina

IPC: G01J3/28 ,  G01B9/02 ,  G01J3/453 ,  G01N21/45 ,  G01J3/02 ,  A61B5/00 ,  G01N21/47

CPC classification number: G01J3/2823 ,  A61B5/445 ,  G01B9/02044 ,  G01B9/02091 ,  G01J3/0218 ,  G01J3/453 ,  G01J3/4531 ,  G01N21/45 ,  G01N21/4795

Abstract: Disclosed herein are systems and methods for deep spectroscopic imaging of a biological sample. In an aspect, a system includes a broad bandwidth light source configured to generate an illumination beam, an interferometer, and a spectrometer. The interferometer includes a first beam splitter configured to split the illumination beam into an incident beam and a reference beam; an optical lens directs the incident beam onto a biological sample at a predefined offset from corresponding optical axis, and receive a beam scattered from the biological sample. The beams are configured to intersect with each other within a focal zone of the optical lens. Photons of the incident beam undergo multiple forward scattering within the biological sample. A second beam splitter configured to receive and superimpose the scattered and reference beams, to generate an interference beam. The spectrometer uses a spectral domain detection technique to assess tissue properties of the biological sample.

公开(公告)号：US09689745B2

公开(公告)日：2017-06-27

申请号：US15050368

申请日：2016-02-22

Applicant: Duke University

Inventor： Adam Wax ,  Robert N. Graf ,  Francisco E. Robles

IPC: G01J3/45 ,  G01B9/02 ,  G01J3/453 ,  G01N21/49

CPC classification number: G01J3/453 ,  G01B9/02044 ,  G01B9/02084 ,  G01B9/02087 ,  G01B9/02091 ,  G01J3/45 ,  G01J2003/4538 ,  G01N21/49

Abstract: Current apparatuses and methods for analysis of spectroscopic optical coherence tomography (SOCT) signals suffer from an inherent tradeoff between time (depth) and frequency (wavelength) resolution. In one non-limiting embodiment, multiple or dual window (DW) apparatuses and methods for reconstructing time-frequency distributions (TFDs) that applies two windows that independently determine the optical and temporal resolution is provided. For example, optical resolution is provided. For example, optical resolution may relate to scattering information about a sample, and temporal resolution may be related to absorption or depth related information. The effectiveness of the apparatuses and methods is demonstrated in simulations and in processing of measured OCT signals that contain fields which vary in time and frequency. The DW technique may yield TFDs that maintain high spectral and temporal resolution and are free from the artifacts and limitations commonly observed with other processing methods.

公开(公告)号：US20130265582A1

公开(公告)日：2013-10-10

申请号：US13868215

申请日：2013-04-23

Applicant: DUKE UNIVERSITY

Inventor： Adam Wax ,  John W. Pyhtila

IPC: G01J3/45

CPC classification number: A61B5/0084 ,  A61B1/00165 ,  A61B1/00172 ,  A61B5/0066 ,  A61B5/0075 ,  G01B9/02043 ,  G01B9/02044 ,  G01B9/02084 ,  G01B9/02087 ,  G01B9/0209 ,  G01B9/02091 ,  G01J3/45 ,  G01N21/31 ,  G01N21/4795 ,  G01N2021/4704 ,  G01N2021/4709 ,  G01N2021/4735 ,  G01N2201/08

Abstract: Fourier domain a/LCI (faLCI) system and method which enables in vivo data acquisition at rapid rates using a single scan. Angle-resolved and depth resolved spectra information is obtained with one scan. The reference arm can remain fixed with respect to the sample due to only one scan required. A reference signal and a reflected sample signal are cross-correlated and dispersed at a multitude of reflected angles off of the sample, thereby representing reflections from a multitude of points on the sample at the same time in parallel. Information about all depths of the sample at each of the multitude of different points on the sample can be obtained with one scan on the order of approximately 40 milliseconds. From the spatial, cross-correlated reference signal, structural (size) information can also be obtained using techniques that allow size information of scatterers to be obtained from angle-resolved data.

Abstract translation: 傅里叶域a / LCI（faLCI）系统和方法，使用单次扫描能够以快速的速率进行体内数据采集。 通过一次扫描获得角度分辨和深度解析的光谱信息。 由于仅需要一次扫描，参考臂可以相对于样品保持固定。 参考信号和反射的采样信号以相对于样本的多个反射角度相互相关并分散，从而同时并行地表示来自样品上的多个点的反射。 可以通过大约40毫秒量级的一次扫描获得关于样本上多个不同点的每个样本的所有深度的信息。 从空间相互关联的参考信号，也可以使用允许从角度分辨数据获得散射体的尺寸信息的技术来获得结构（尺寸）信息。

公开(公告)号：US12171524B2

公开(公告)日：2024-12-24

申请号：US17589333

申请日：2022-01-31

Applicant: DUKE UNIVERSITY ,  THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

Inventor： Adam Wax ,  Nicholas Shaheen ,  Kengyeh Chu ,  Derek Ho ,  Zachary Steelman

IPC: A61B5/00 ,  A61B1/018 ,  A61B1/05 ,  A61B1/06 ,  A61B1/07 ,  A61B10/04 ,  A61B18/00 ,  A61B18/14 ,  G01B9/02091

Abstract: Systems and methods for multi-modal imaging using an endoscope having an instrument channel, where the imaging is achieved without using the channel, are disclosed. The systems can include a multi-modal imaging paddle housing couple to a distal end of the endoscope. The housing can receive at least two imaging probes. The imaging probes can be an angle-resolved low-coherence interferometry probe (a/LCI) and an optical coherence tomography (OCT) probe. The housing can be scaled and positioned to be visible via the endoscope camera. The system and method can include locating the housing in a region of interest using the endoscope camera, acquiring OCT measurements to identify targets, and then acquiring a/LCI measurements at the identified targets.

公开(公告)号：US11828593B2

公开(公告)日：2023-11-28

申请号：US17867425

申请日：2022-07-18

Applicant: Duke University

Inventor： Adam Wax ,  Michael Crose ,  William Brown

IPC: G01B9/02091 ,  A61B3/12 ,  A61B5/00 ,  G01J3/02 ,  G01J3/45

CPC classification number: G01B9/02091 ,  A61B3/12 ,  A61B5/0035 ,  A61B5/0066 ,  G01J3/0208 ,  G01J3/45

Abstract: An optical coherence tomography (OCT) scan device includes an OCT scan device housing, an interferometer disposed within the OCT scan device housing and including a light source, a fiber optic coupler including an interferometer output, a reference-arm, and a sample-arm. The OCT scan device further includes a power source configured to provide power to the light source and the remaining components of the OCT scan device, and a controller disposed within the OCT scan device housing and configured to adjust lens focusing parameters in the reference-arm and the sample-arm, and control a scanning function of an optical beam emitting from the sample-arm. The OCT scan device is further configured to transmit and receive control instructions and transmit fundus image data.