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公开(公告)号：US09297016B2
公开(公告)日：2016-03-29
申请号：US13029558
申请日：2011-02-17
申请人： Dennis Flint , Brian James Paul , Rick W. Ye
发明人： Dennis Flint , Brian James Paul , Rick W. Ye
IPC分类号： C12N15/81 , C07K14/395 , C12N9/88 , C12P7/16 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , G01N33/573
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12N15/81 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , C12Y402/01009 , G01N33/573 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
摘要翻译： 本发明涉及包含二羟基酸脱水酶多肽的重组宿主细胞，特别是酵母细胞。 本发明还涉及由于多肽的表达增加，细胞的Fe-S簇生物合成的调节或其组合而导致的具有增加的二羟基酸脱水酶多肽的比活性的重组宿主细胞。 本发明还包括使用宿主细胞的方法，以及用于鉴定增加宿主细胞中Fe-S簇生物合成途径中的通量的多肽的方法。
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公开(公告)号：US20120064561A1
公开(公告)日：2012-03-15
申请号：US13029558
申请日：2011-02-17
申请人： DENNIS FLINT , Brian James Paul , Rick W. Ye
发明人： DENNIS FLINT , Brian James Paul , Rick W. Ye
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12N15/81 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , C12Y402/01009 , G01N33/573 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
摘要翻译： 本发明涉及包含二羟基酸脱水酶多肽的重组宿主细胞，特别是酵母细胞。 本发明还涉及由于多肽的表达增加，细胞的Fe-S簇生物合成的调节或其组合而导致的具有增加的二羟基酸脱水酶多肽的比活性的重组宿主细胞。 本发明还包括使用宿主细胞的方法，以及用于鉴定增加宿主细胞中Fe-S簇生物合成途径中的通量的多肽的方法。
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3.发明申请FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY EFFECTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES 审中-公开使用高效的乙醇酸还原酶制剂生产ISOBUTMOL公开(公告)号：US20110244536A1
公开(公告)日：2011-10-06
申请号：US12893077
申请日：2010-09-29
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/88 , C12N15/52 , C12P7/42 , C12Y101/01008 , C12Y101/01086 , C12Y401/01001 , C12Y401/01072 , C12Y401/02009 , C12Y402/01009 , Y02E50/10 , Y02P20/52
摘要： Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.
摘要翻译： 已经鉴定了酮醇酸还原异构酶，其在细菌和酵母中作为异丁醇生物合成途径的步骤在体内提供高效力。 这些KARI是通过称为SLSL分子的分子系统发育分析鉴定的进化枝的成员。
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公开(公告)号：US09238828B2
公开(公告)日：2016-01-19
申请号：US13560147
申请日：2012-07-27
申请人： Jessica McElvain , Daniel P O'Keefe , Brian James Paul , Mark S Payne , Steven Cary Rothman , Hongxian He
发明人： Jessica McElvain , Daniel P O'Keefe , Brian James Paul , Mark S Payne , Steven Cary Rothman , Hongxian He
CPC分类号： C12P7/16 , C12N9/0006 , C12P7/24 , Y02E50/10
摘要： Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.
摘要翻译： 本文提供了编码具有酮异戊酸脱羧酶活性的多肽的多肽和多核苷酸。 还提供了包含这种多肽和多核苷酸的重组宿主细胞及其使用方法。
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公开(公告)号：US08455224B2
公开(公告)日：2013-06-04
申请号：US12569136
申请日：2009-09-29
申请人： Brian James Paul
发明人： Brian James Paul
CPC分类号： C12N15/746 , C12N9/0006 , C12P7/16 , C12P7/18 , C12P7/26 , C12Y101/02004 , Y02E50/10
摘要： A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.
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公开(公告)号：US09169499B2
公开(公告)日：2015-10-27
申请号：US12893065
申请日：2010-09-29
申请人： Brian James Paul , Wonchul Suh
发明人： Brian James Paul , Wonchul Suh
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12N9/88 , C12N15/746 , C12Y101/01027 , C12Y203/01054 , C12Y401/01005 , Y02E50/10
摘要： An engineering method was developed to allow genetic modification and isolation of lactic acid bacteria cells that lack lactate dehydrogenase and acetolactate decarboxylase activities. In cells with these modifications and an isobutanol biosynthetic pathway, improved production of isobutanol was observed.
摘要翻译： 开发了一种工程方法，允许遗传修饰和分离缺乏乳酸脱氢酶和乙酰乳酸脱羧酶活性的乳酸菌细胞。 在具有这些修饰和异丁醇生物合成途径的细胞中，观察到改善的异丁醇生产。
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7.发明申请KETO-ISOVALERATE DECARBOXYLASE ENZYMES AND METHODS OF USE THEREOF 有权KETO-ISOVALERATE DECARBOXYLASE ENZYMES及其使用方法公开(公告)号：US20130203138A1
公开(公告)日：2013-08-08
申请号：US13560147
申请日：2012-07-27
申请人： Jessica McElvain , Daniel P. Okeefe , Brian James Paul , Mark S. Payne , Steven Cary Rothman , Hongxian He
发明人： Jessica McElvain , Daniel P. Okeefe , Brian James Paul , Mark S. Payne , Steven Cary Rothman , Hongxian He
CPC分类号： C12P7/16 , C12N9/0006 , C12P7/24 , Y02E50/10
摘要： Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.
摘要翻译： 本文提供了编码具有酮异戊酸脱羧酶活性的多肽的多肽和多核苷酸。 还提供了包含这种多肽和多核苷酸的重组宿主细胞及其使用方法。
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公开(公告)号：US08637281B2
公开(公告)日：2014-01-28
申请号：US12569168
申请日：2009-09-29
申请人： Brian James Paul , Wonchul Suh
发明人： Brian James Paul , Wonchul Suh
摘要： Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 μmol min−1 mg−1. LAB cells with even higher activities of 0.2 to 0.6 μmol min−1 mg−1 of DHAD activity were obtained.These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.
摘要翻译： 修饰乳酸菌（LAB）细胞使其具有增加至约0.1mumol / min-1mg-1的二羟基酸脱水酶活性的比活性。 获得具有0.2至0.6mumol min-1mg-1的DHAD活性的更高活性的LAB细胞。 当额外的异丁醇生物合成途径酶被表达时，这些修饰的细胞可用于产生异丁醇。
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公开(公告)号：US20110136192A1
公开(公告)日：2011-06-09
申请号：US12893065
申请日：2010-09-29
申请人： Brian James Paul , Wonchul Suh
发明人： Brian James Paul , Wonchul Suh
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12N9/88 , C12N15/746 , C12Y101/01027 , C12Y203/01054 , C12Y401/01005 , Y02E50/10
摘要： An engineering method was developed to allow genetic modification and isolation of lactic acid bacteria cells that lack lactate dehydrogenase and acetolactate decarboxylase activities. In cells with these modifications and an isobutanol biosynthetic pathway, improved production of isobutanol was observed.
摘要翻译： 开发了一种工程方法，允许遗传修饰和分离缺乏乳酸脱氢酶和乙酰乳酸脱羧酶活性的乳酸菌细胞。 在具有这些修饰和异丁醇生物合成途径的细胞中，观察到改善的异丁醇生产。
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10.发明申请CARBON PATHWAY OPTIMIZED PRODUCTION HOSTS FOR THE PRODUCTION OF ISOBUTANOL 审中-公开碳通道优化生产ISOBUTANOL的生产主体公开(公告)号：US20100120105A1
公开(公告)日：2010-05-13
申请号：US12606410
申请日：2009-10-27
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1205 , C12N9/18 , C12N9/88 , C12N9/92 , C12N15/52 , C12Y101/01044 , C12Y101/01049 , C12Y207/02011 , C12Y401/02014 , C12Y402/02012 , Y02E50/10
摘要： A microbial host cell is provided for the production of isobutanol. Carbon flux in the cell is optimized through the Entner-Doudoroff pathway.
摘要翻译： 提供用于生产异丁醇的微生物宿主细胞。 通过Entner-Doudoroff途径优化细胞中的碳通量。
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