公开(公告)号：US06498017B2

公开(公告)日：2002-12-24

申请号：US09508959

申请日：2000-05-22

申请人： Detlev Riesner ,  Karin Post ,  Oliver Schaefer ,  Martin Pitschke ,  Manfred Eigen ,  Jan Bieschke

发明人： Detlev Riesner ,  Karin Post ,  Oliver Schaefer ,  Martin Pitschke ,  Manfred Eigen ,  Jan Bieschke

IPC分类号： C12Q137

CPC分类号： G01N33/6896

摘要： A method for the diagnostic detection of diseases associated with protein depositions (pathological protein depositions) by measuring an association of substructures of the pathological protein depositions, structures forming pathological protein depositions, structures corresponding to pathological protein depositions and/or pathological protein depositions as a probe; to substructures of the pathological protein depositions structures forming pathological protein depositions, structures corresponding to pathological protein deposition and/or pathological protein depositions as targets; characterized in that the target is detected in liquid phase wherein, in the case of detecting Alzheimer's disease, the liquid phase is obtained from body fluids or is itself a body fluid; with the proviso that the association of the probe to the target is measured before self-aggregation of the probe predominates.

摘要翻译： 通过测量病理蛋白沉积的亚结构，形成病理蛋白沉积的结构，对应于病理蛋白沉积的结构和/或病理蛋白沉积作为探针来诊断与蛋白质沉积相关的疾病（病理蛋白沉积）的诊断方法 ;形成病理蛋白沉积的病理蛋白沉积结构的亚结构，对应于病理蛋白沉积和/或病理蛋白沉积的靶标的结构;其特征在于在液相中检测靶，其中在检测阿尔茨海默病的情况下， 液相是从体液中获得的，或者本身是体液;条件是在探针的自身聚集占优势之前测量探针与靶标的结合。

公开(公告)号：US5871908A

公开(公告)日：1999-02-16

申请号：US157195

申请日：1993-12-08

申请人： Karsten Henco ,  Manfred Eigen ,  Detlev Riesner

发明人： Karsten Henco ,  Manfred Eigen ,  Detlev Riesner

IPC分类号： B01L7/00 ,  C12Q1/68 ,  C12P19/34

CPC分类号： B01L7/52 ,  C12Q1/6825 ,  C12Q1/686

摘要： A process for the qualitative and quantitative determination of at least one in vitro amplified nucleic acid in a sealed reaction chamber,wherein during or subsequent to the amplification of the nucleic acid at least one substance (probe) is present which interacts with the nucleic acid to be detected;wherein spectroscopically measurable parameters of said substance (probe) are subject to variation, creating a measurable signal;wherein the sample to be measured is exposed to the action of a gradient capable of at least partially denaturing nucleic acids;with detection of the measurable parameter undergoing variation through the action of the gradient; andthe entire amplification reaction, including qualitative and quantitative determination, may be carried out in a sealed reaction chamber (measuring compartment) without intermittent opening,permitting to automatically operate the diagnostic method of DNA and RNA amplification in qualitative and quantitative fashion on large series of samples.

摘要翻译： PCT No.PCT / EP93 / 00254 Sec。 371日期：1993年12月8日 102（e）日期1993年12月8日PCT提交1993年2月4日PCT公布。 公开号WO93 / 16194 日期1993年8月19日一种在密封反应室中定性和定量测定至少一种体外扩增的核酸的方法，其中在核酸扩增期间或之后存在至少一种物质（探针），其与 与待检测的核酸; 其中所述物质（探针）的光谱可测量参数经历变化，产生可测量的信号; 其中要测量的样品暴露于能够至少部分变性核酸的梯度的作用; 通过梯度的作用检测可测量的参数变化; 并且包括定性和定量测定的整个扩增反应可以在密封的反应室（测量室）中进行，而不间断地打开，允许以定性和定量的方式自动操作DNA和RNA扩增的诊断方法 样品。

公开(公告)号：US20090042211A1

公开(公告)日：2009-02-12

申请号：US11994727

申请日：2006-07-04

申请人： Eva Birkmann ,  Detlev Riesner ,  Nicole Weinmann ,  Oliver Schafer

发明人： Eva Birkmann ,  Detlev Riesner ,  Nicole Weinmann ,  Oliver Schafer

IPC分类号： G01N33/53 ,  G01N33/566

CPC分类号： G01N33/6896 ,  B01D15/3804 ,  B01J2220/54 ,  G01N33/543 ,  G01N33/582 ,  G01N2333/4709 ,  G01N2800/2828

摘要： The invention relates to a method for the selective detection of the presence and/or quantity of pathological protein depositions.

摘要翻译： 本发明涉及用于选择性检测病理蛋白沉积的存在和/或数量的方法。

公开(公告)号：US20090118470A1

公开(公告)日：2009-05-07

申请号：US12064682

申请日：2006-08-24

申请人： Detlev Riesner ,  Henrik Muller

发明人： Detlev Riesner ,  Henrik Muller

IPC分类号： C07K1/00

CPC分类号： C07K14/47

摘要： Subject of the invention is a process for the inactivation of prions in a sample which comprises prion proteins or is suspected of comprising prion proteins, the process comprising a heat treatment in the presence of a lipophilic substance comprising at least 8 carbon atoms, wherein the heat treatment is performed at a temperature below 100° C. at which the lipophilic substance is in a liquid state and under conditions wherein degradation of less than 90% of the prion proteins occurs.

摘要翻译： 本发明的主题是在包含朊病毒蛋白或怀疑含有朊病毒蛋白的样品中灭活朊病毒的方法，所述方法包括在包含至少8个碳原子的亲脂性物质存在下的热处理，其中所述热 在低于100℃的温度下进行处理，在该温度下亲脂性物质处于液体状态，并且发生少于90％的朊蛋白质降解的条件下进行。

公开(公告)号：US4076420A

公开(公告)日：1978-02-28

申请号：US498816

申请日：1974-08-19

申请人： Leo C. M. De Maeyer ,  Carl-Roland Rabl ,  Thomas M. Jovin ,  Detlev Riesner ,  Rudolf Rigler ,  Lutz Bodo Veil ,  Hans Lehrach

发明人： Leo C. M. De Maeyer ,  Carl-Roland Rabl ,  Thomas M. Jovin ,  Detlev Riesner ,  Rudolf Rigler ,  Lutz Bodo Veil ,  Hans Lehrach

IPC分类号： G01N21/25 ,  G01N21/27 ,  G01N21/00 ,  G01J3/30 ,  G01N1/10

CPC分类号： G01N21/272 ,  G01N21/253

摘要： A reaction kinetics measuring system which provides for the application of great variety of reaction initiating methods, as temperature or pressure or electrical field jump methods, mixing methods, flash photolysis method, and combinations of said methods, and for various optical detection methods, as absorption or fluorescence or scattered light or polarized light detection methods. The system features quickly and easily interchangeable optical elements in a number of optical paths, and a multitude of selectively usable measuring cells to adapt the system to the reaction initiating and detection methods used so that reliable and exactly reproducible results are obtained, which are comparable even if obtained under widely differing conditions.

摘要翻译： 一种反应动力学测量系统，其提供了多种反应引发方法，如温度或压力或电场跃迁方法，混合方法，闪光光解法和所述方法的组合，并且用于各种光学检测方法，如 吸收或荧光或散射光或偏振光检测方法。 该系统在许多光路中具有快速且容易的可互换的光学元件，以及多个可选择使用的测量单元，以使系统适应于所使用的反应起始和检测方法，使得获得可靠且精确可重现的结果，甚至可比较 如果在广泛不同的条件下获得。

公开(公告)号：US20050239808A1

公开(公告)日：2005-10-27

申请号：US10514015

申请日：2003-05-09

申请人： Thomas Schrader ,  Detlev Riesner ,  Petra Rzepecki ,  Luitgard Nagel-Steger ,  Mark Wehner ,  Christian Kirsten ,  Oliver Molt ,  Reza Zadmard ,  Katja Aschermann

发明人： Thomas Schrader ,  Detlev Riesner ,  Petra Rzepecki ,  Luitgard Nagel-Steger ,  Mark Wehner ,  Christian Kirsten ,  Oliver Molt ,  Reza Zadmard ,  Katja Aschermann

IPC分类号： A61P25/28 ,  C07D231/40 ,  C07D401/14 ,  A61K31/522 ,  A61K31/416 ,  A61K31/4196 ,  A61K31/4709 ,  C07D473/02 ,  C07D43/02

CPC分类号： C07D231/40 ,  C07D401/14

摘要： The invention on hand refers to diseases associated with abnormal protein structures, including Alzheimer's disease, Creutzfeldt-Jakob's disease, BSE and other prion-associated diseases. It is the task of the invention on hand to provide new active agents preventing the formation of amyloid plaques and to dissolve existing plaques, such agents being convenient for therapy, diagnosis and prophylaxis of diseases that are associated with abnormal protein structures. This task is performed in terms of the current invention by heterocyclic or aromatic agents with a rigid structure and a donor-acceptor-donor pattern (DAD), the latter being formed by donors and acceptors of hydrogen bridge linkages which comply with the β-sheet structure of the peptide or protein and thus fit as binding partners. The heterocyclic or aromatic agents are available at least as dimers. They recognise peptides and proteins with a β-sheet structure, form stable complexes with them and inhibit their aggregation to β-amyloid plaques. Furthermore, the new active agents are able to dissolve β-amyloid plaques that already exist. Another task of the current invention is to provide methods for the synthesis of said heterocyclic or aromatic compounds.

摘要翻译： 手头的发明是指与异常蛋白质结构相关的疾病，包括阿尔茨海默病，克雅氏病，疯牛病和其他朊病毒相关疾病。 本发明的任务是提供防止淀粉样蛋白斑块形成和溶解现有斑块的新的活性剂，所述药剂便于治疗，诊断和预防与异常蛋白质结构相关的疾病。 根据本发明，通过具有刚性结构和供体 - 受体 - 给体模式（DAD）的杂环或芳族化合物来执行该任务，后者由供体和符合β-折叠板的氢桥连接体的受体形成 肽或蛋白质的结构，因此适合作为结合配偶体。 杂环或芳族试剂至少可以作为二聚体获得。 它们识别具有β-折叠结构的肽和蛋白质，与它们形成稳定的复合物并抑制它们与β-淀粉样蛋白斑的聚集。 此外，新的活性剂能够溶解已经存在的β-淀粉样斑块。 本发明的另一个任务是提供合成所述杂环或芳族化合物的方法。

公开(公告)号：US5795720A

公开(公告)日：1998-08-18

申请号：US558594

申请日：1995-10-31

申请人： Karsten Henco ,  Detlev Riesner ,  Gerhard Steger

发明人： Karsten Henco ,  Detlev Riesner ,  Gerhard Steger

IPC分类号： B01D57/02 ,  C12Q1/68 ,  G01N27/447

CPC分类号： B01D57/02 ,  C12Q1/6827 ,  G01N27/44704

摘要： The invention relates to a process for the separation and detection of components of a mixture of materials by temperature gradient gel electrophoresis, wherein either a spatial temperature gradient is built up by spatially separated temperature levels, or a time temperature gradient, or a temperature gradient is built up by combination of spatial and timewise temperature gradient. The temperature levels for building up the spatial temperature gradient are adjusted by controllable heating or cooling devices. To build up the time temperature gradient, the temperature level at each point of the separation path within the separation medium may be optionally adjusted time-dependently by means of controllable heating or cooling devices. There is described a device for performing the process with controllable heating or cooling devices to build up temperature gradients, a hollow body arranged between the temperature levels which contains the medium used for separation, and a thermostat jacket enclosing the hollow body.

摘要翻译： 本发明涉及通过温度梯度凝胶电泳分离和检测材料混合物的组分的方法，其中通过空间分离的温度水平或时间温度梯度或温度梯度来建立空间温度梯度 通过空间和时间温度梯度的组合建立。 通过可控制的加热或冷却装置调节用于建立空间温度梯度的温度水平。 为了建立时间温度梯度，分离介质内的分离路径的每个点处的温度水平可以可选地通过可控制的加热或冷却装置随时调整。 描述了一种用可控制的加热或冷却装置执行该过程的装置，以建立温度梯度，布置在包含用于分离的介质的温度水平之间的空心体和包围中空体的恒温套管。

公开(公告)号：US5066377A

公开(公告)日：1991-11-19

申请号：US545111

申请日：1990-06-29

申请人： Volker Rosenbaum ,  Detlev Riesner

发明人： Volker Rosenbaum ,  Detlev Riesner

IPC分类号： B01D43/00 ,  B01D57/02 ,  B01L7/00 ,  C02F9/00 ,  G01N27/26 ,  G01N27/447 ,  G01N30/30 ,  G01N30/90

CPC分类号： C02F9/005 ,  B01D43/00 ,  B01D57/02 ,  B01L7/54 ,  G01N27/44704 ,  G01N30/90 ,  G01N2030/3015 ,  G01N2030/3023 ,  G01N2030/303

摘要： A method and a device for producing a controllable and reproducible temperature gradient in a predetermined direction of heat-conducting plates for the separation of mixtures of substances in sheet-shaped separating media wherein one edge of a plate is heated by means of one or more controllable heating means and the opposite edge of the plate is cooled by one or more controllable cooling means. In this way, the energy flows through the heating means and of the cooling means are greater than the energy flow across the plate. The method and device are suitable for separating mixtures of substances wherein at least one component undergoes a thermal conversion within the temperature range of the temperature gradient. Therefore, they are suitable for the detection of and differentiation between viroids, satellite RNAs, viruses containing double-strand or circular nucleic as well as for the analysis of protein-nucleic acid complexes and for the analysis of mutations.

摘要翻译： 一种用于在导热板的预定方向上产生可控和可再现的温度梯度的方法和装置，用于分离片状分离介质中物质的混合物，其中板的一个边缘通过一个或多个可控制的 加热装置和板的相对边缘由一个或多个可控冷却装置冷却。 以这种方式，通过加热装置和冷却装置的能量流过跨越板的能量流。 该方法和装置适于分离物质的混合物，其中至少一种成分在温度梯度的温度范围内经历热转化。 因此，它们适用于检测和分化病毒，卫星RNA，含有双链或环状核酸的病毒以及蛋白质 - 核酸复合物的分析和突变分析。

公开(公告)号：US20060281676A1

公开(公告)日：2006-12-14

申请号：US10570233

申请日：2004-08-04

申请人： Dieter Willbold ,  Jorg Smolinski ,  Karen Hanel ,  Michael Wolff ,  Detlev Riesner ,  Carsten Korth

发明人： Dieter Willbold ,  Jorg Smolinski ,  Karen Hanel ,  Michael Wolff ,  Detlev Riesner ,  Carsten Korth

IPC分类号： A61K38/17 ,  C07K14/47 ,  C07H21/04 ,  C12P21/06

CPC分类号： A61K38/05

摘要： The invention relates to an agent and/or pharmaceutical active ingredient and to a method for the treatment, prevention and diagnosis of TSE, and to a method for the production of an agent and/or pharmaceutical active ingredient. According to the invention, peptides and/or nucleotide sequences coding for said peptides are provided as disclosed in the sequence protocols 127 and partial sequences thereof are disclosed. Treatment or prevention of the TSE disease, for example, in humans with Jacob Creutzfeldt syndrome, kuru, Gertmann Sträussler-Scheinker syndrome and FFI (fatal familial insomnia) can be carried out with the aid of the inventive peptides. TSE diseases are also known in animals, such as Scrapie in sheep, BSE in cows and CWD in wild animals.

摘要翻译： 本发明涉及一种药剂和/或药物活性成分以及用于治疗，预防和诊断TSE的方法，以及用于制备药剂和/或药物活性成分的方法。 根据本发明，编码所述肽的肽和/或核苷酸序列如序列方案127中所公开的那样提供，并且公开了其部分序列。 借助于本发明的肽，可以进行治疗或预防例如在Jacob Creutzfeldt综合征，kuru，GertmannSträussler-Scheinker综合征和FFI（致命家族性失眠）的人中的TSE疾病。 TSE疾病在动物中也是已知的，例如羊中的Scrapie，牛中的疯牛病和野生动物中的CWD。

公开(公告)号：US4699717A

公开(公告)日：1987-10-13

申请号：US830708

申请日：1986-02-14

申请人： Detlev Riesner ,  Metin Colpan

发明人： Detlev Riesner ,  Metin Colpan

IPC分类号： B01D15/08 ,  B01J20/28 ,  B01J20/32

CPC分类号： B01D15/08 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28057 ,  B01J20/28085 ,  B01J20/28095 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3257 ,  B01J20/3259 ,  B01J20/3263 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01J2220/54 ,  B01J2220/58

摘要： A process for the chromatographic separation of nucleic acid using a chromatographic carrier material is described in which the surface of the carrier material is specially modified.

摘要翻译： 描述了使用色谱载体材料色谱分离核酸的方法，其中载体材料的表面被特别改性。