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    .gamma.-Glutamyl-4-nitroanilide compounds
    2.
    发明授权
    .gamma.-Glutamyl-4-nitroanilide compounds 失效
    {65-氨基-4-硝基苯胺化合物

    公开(公告)号：US4049702A

    公开(公告)日：1977-09-20

    申请号：US589653

    申请日：1975-06-23

    申请人： Erich Bernt ,  Wolfgang Gruber ,  Erich Haid ,  Fritz Stahler ,  August Wilhelm Wahlefeld ,  Gunter Weimann

    发明人： Erich Bernt ,  Wolfgang Gruber ,  Erich Haid ,  Fritz Stahler ,  August Wilhelm Wahlefeld ,  Gunter Weimann

    IPC分类号： C12Q1/48 ,  C07C143/67

    CPC分类号： C12Q1/48

    摘要： .gamma.-Glutamyl-4-nitroanilide compounds of the formula ##STR1## wherein R is a .gamma.-glutamyl radical and X is an acid group, and salts of such compounds, are used to determine .gamma.-glutamyl-transpeptidase by contacting a sample suspected of containing .gamma.-glutamyltranspeptidase with said compound and glycylglycine and determining the liberation of the resulting p-nitro-aniline compound as a measure of the .gamma.-glutamyltranspeptidase activity initially present in the sample.

    摘要翻译： 其中R是γ-谷氨酰基，X是酸基的γ-谷氨酰基-4-硝基苯胺化合物和这些化合物的盐被用于通过使怀疑有γ-谷氨酰转肽酶的样品接触来测定γ-谷氨酰转肽酶 含有γ-谷氨酰基转肽酶与所述化合物和甘氨酰甘氨酸，并确定所得对硝基苯胺化合物的释放作为样品中最初存在的γ-谷氨酰转肽酶活性的量度。

    Method and reagent for the determination of creatine kinase
    3.
    发明授权
    Method and reagent for the determination of creatine kinase 失效
    用于测定肌酸激酶的方法和试剂

    公开(公告)号：US4286057A

    公开(公告)日：1981-08-25

    申请号：US125381

    申请日：1980-02-28

    申请人： Karl Wulff ,  Fritz Stahler ,  Wolfgang Gruber

    发明人： Karl Wulff ,  Fritz Stahler ,  Wolfgang Gruber

    IPC分类号： C12Q1/42 ,  C12Q1/50 ,  C12Q1/66

    CPC分类号： C12Q1/66 ,  C12Q1/50 ,  Y10S435/81

    摘要： Creatine kinase is determined by the reaction of creatine phosphate with adenosine diphosphate with the formation of adenosine triphosphate, reaction of the latter with luciferin and oxygen in the presence of luciferase and diadenosine pentaphosphate with the formation of oxyluciferin and adenosine monophosphate, and measurement of the light thereupon emitted, the reaction being performed with a saturated concentration of adenosine diphosphate and substrate, in the presence of 1 to 10 millimoles per liter of AMP at pH values of 5.8 to 7.5, with at least 50 units of luciferase per test. It is desirable to operate in the presence of diadenosine pentaphosphate or sodium fluoride and a sequestering agent.

    摘要翻译： 肌酸激酶由磷酸肌酸磷酸腺苷与三磷酸腺苷的反应决定，后者与荧光素酶和氧在荧光素酶和二腺苷酸五磷酸的存在下反应，形成荧光素和磷酸腺苷，并测量光 随后发射，反应在饱和浓度的二磷酸腺苷和底物的存在下，在pH值为5.8至7.5的每升AMP存在1至10毫摩尔，每个试验至少50单位荧光素酶。 需要在二膦酸二钙或氟化钠和螯合剂的存在下进行操作。

    Process for carrying out analytical determinations and means for carrying out this process
    7.
    发明授权
    Process for carrying out analytical determinations and means for carrying out this process 失效
    进行分析确定的过程和进行此过程的手段

    公开(公告)号：US4898832A

    公开(公告)日：1990-02-06

    申请号：US147610

    申请日：1988-01-25

    申请人： Sigmar Klose ,  Fritz Stahler

    发明人： Sigmar Klose ,  Fritz Stahler

    IPC分类号： G01N21/78 ,  B04B5/04 ,  C12Q1/00 ,  G01N33/53

    CPC分类号： B01L3/5027 ,  B04B5/0407 ,  C12Q1/00 ,  G01N33/5302 ,  B01L2200/16 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L3/502738 ,  Y10T436/111666

    摘要： A process for carrying out an analytical determination by mixing and incubating a sample solution with at least one reagent and measuring a parameter in the reaction mixture, the sample solution being transported from an application point to a measurement point, wherein a sample solution is first transported to a soluble dry reagent, with at least partial dissolving of the latter, and then further transported to a measurement point, the transport taking place by two different forces, whereby, at least on a part of the transport path, it is brought about by a surface force acting on the solution as a first force, which, for the regulation of the transport velocity or transport direction, is superimposed by a centrifugal force and/or pressure force as a second force which, depending upon which transport state of the fluid is to be adjusted, is made greater or smaller than the first force.

    摘要翻译： 一种用于通过将样品溶液与至少一种试剂混合并培养并测量反应混合物中的参数进行分析测定的方法，所述样品溶液从应用点运送到测量点，其中样品溶液首先运输 到可溶性干试剂，至少部分溶解后者，然后进一步输送到测量点，运输通过两个不同的力进行，由此至少在输送路径的一部分上由 作为第一力的作用于溶液的表面力，其用于调节输送速度或输送方向，通过作为第二力的离心力和/或压力力叠加，这取决于流体的哪个输送状态 要被调整，比第一力更大或更小。