公开(公告)号：US20180202981A1

公开(公告)日：2018-07-19

申请号：US15743460

申请日：2016-07-14

Applicant: VRIJE UNIVERSITEIT BRUSSEL

Inventor： Wim DE MALSCHE

IPC: G01N30/38 ,  G01N30/32

CPC classification number: G01N30/38 ,  G01N30/32 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/326 ,  G01N2030/387

Abstract: A chromatographic column assembly is described, comprising a channel and a flow inducer. The channel comprises an inlet for receiving a sample liquid and an outlet, and is adapted for separating the sample liquid into components when the sample liquid flows through the channel in an axial direction from the inlet to the outlet. The flow inducer is adapted for controllably inducing, when the sample fluid is flowing through the channel, a motion of the sample liquid in the channel in a plane substantially orthogonal to the axial direction.

公开(公告)号：US20070084727A1

公开(公告)日：2007-04-19

申请号：US11249383

申请日：2005-10-14

Applicant: Eric Cummings

Inventor： Eric Cummings

IPC: C07K1/26 ,  G01N27/00

CPC classification number: G01N30/0005 ,  B82Y15/00 ,  G01N30/6065 ,  G01N2030/0065 ,  G01N2030/387

Abstract: Disclosed herein are methods and devices for coherent nonlinear chromatography. As disclosed, the devices comprise microchannels having at least one perturber which produces a non-uniformity in a field spanning the width of the microchannel. The interaction of the field non-uniformity with a particle produces a secondary flow or particle motion component which competes with a primary flow. Depending on the interaction, the particle may be retained and redirected and therefore separated from other particles.

Abstract translation: 本文公开了用于相干非线性色谱法的方法和装置。 如所公开的，这些装置包括具有至少一个扰流器的微通道，其在跨越微通道宽度的场中产生不均匀性。 场不均匀性与粒子的相互作用产生与主流相竞争的二次流或粒子运动分量。 取决于相互作用，颗粒可被保留并重定向并因此与其它颗粒分离。

公开(公告)号：US20120003676A1

公开(公告)日：2012-01-05

申请号：US13232861

申请日：2011-09-14

Applicant: William F. Haddon ,  Nigel J. Clarke

Inventor： William F. Haddon ,  Nigel J. Clarke

IPC: C12Q1/48 ,  H01J49/26

CPC classification number: B01D59/44 ,  G01N30/7233 ,  G01N33/6848 ,  G01N2030/387 ,  G01N2030/8831

Abstract: Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.

Abstract translation: 描述了用于测量样品中甲基化TPMT酶产物的量的方法。 更具体地，描述了用于在测试样品中利用质谱技术检测和定量6-MMP或同位素标记的6-MMP的质谱法，并且使用这些方法来测定样品中存在的TPMT酶的活性。

公开(公告)号：US20020158022A1

公开(公告)日：2002-10-31

申请号：US10118469

申请日：2002-04-05

Applicant: Fluidigm Corporation

Inventor： Jiang Huang ,  Hou-Pu Chou ,  Marc A. Unger

IPC: B01D015/08

CPC classification number: B01D15/08 ,  G01N30/6065 ,  G01N30/6095 ,  G01N2030/387 ,  G01N2030/521 ,  Y10T137/86863 ,  Y10T436/2575

Abstract: The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

Abstract translation: 本发明涉及包含微流体输送系统和与流体输送系统流体连通的色谱柱的微流体色谱装置及其制造和使用方法。 优选地，色谱柱包括OTLC，PCLC或其组合。

公开(公告)号：US6149816A

公开(公告)日：2000-11-21

申请号：US476257

申请日：2000-01-03

Applicant: Hubert M. Quinn ,  John E. Brann, III

Inventor： Hubert M. Quinn ,  John E. Brann, III

IPC: B01J20/28 ,  B01J20/282 ,  B01J20/287 ,  G01N30/32 ,  G01N30/38 ,  G01N30/52 ,  G01N30/72 ,  G01N30/88 ,  B01D15/08

CPC classification number: B01J20/282 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28078 ,  B01J20/283 ,  B01J20/287 ,  G01N30/38 ,  G01N30/482 ,  B01J2220/54 ,  B01J2220/58 ,  G01N2030/324 ,  G01N2030/387 ,  G01N2030/524 ,  G01N2030/525 ,  G01N2030/8813 ,  G01N2030/884 ,  G01N30/7233

Abstract: A system for separating compounds of relatively low molecular weight substantially not greater than about one kilodalton from compounds having relatively high molecular weights substantially an order of magnitude greater or more than the low molecular weight compounds in a liquid mixture. The system includes a chromatographic column packed with uniformly distributed rigid, solid, porous particles having chromatographically active, hydrophobic surfaces, average diameters of not less than about 30 .mu.m, and average pore diameters sufficiently small to substantially exclude introduction of the compounds of relatively high molecular weight into the pores. The mixture is pumped through the interstitial volume between the particles at a reduced velocity greater than about 5,000, until a band of the high molecular weight compounds exits the column. The low molecular weight compounds are then eluted and are recovered separately from the relatively high molecular weight compounds. Spectrographic identification of the recovered low molecular weight compounds can then be made, preferably by ultraviolet absorption or by mass spectrograph.

Abstract translation: 从具有相对较高分子量的化合物分离基本上不大于约1千道尔顿的相对低分子量的化合物的系统，其在液体混合物中基本上比低分子量化合物大一或多数量级。 该系统包括一个色谱柱，填充均匀分布的刚性，固体，多孔的颗粒，具有色谱活性，疏水性表面，平均直径不小于约30微米，平均孔径足够小，基本上不排除相对较高的化合物的引入 分子量进入毛孔。 将混合物以大于约5,000的减小的速度泵送通过颗粒之间的间隙体积，直到高分子量化合物的带离开柱。 然后洗脱低分子量化合物，并从相对高分子量的化合物中单独回收。 然后可以优选通过紫外吸收或通过质谱仪来制备回收的低分子化合物的光谱鉴定。

公开(公告)号：US4515889A

公开(公告)日：1985-05-07

申请号：US323205

申请日：1981-11-20

Applicant: Sigmar Klose ,  Fritz Stahler ,  Hans Lange ,  Wolfgang Kleemann

Inventor： Sigmar Klose ,  Fritz Stahler ,  Hans Lange ,  Wolfgang Kleemann

IPC: G01N33/48 ,  B01F5/06 ,  B01F13/00 ,  B04B5/04 ,  C12Q1/00 ,  G01N21/07 ,  G01N30/02 ,  G01N30/34 ,  G01N30/38 ,  G01N30/74 ,  G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N33/54

CPC classification number: G01N21/07 ,  B01F15/0233 ,  B01F5/064 ,  B01F5/0682 ,  B01F5/0694 ,  C12Q1/00 ,  G01N33/5302 ,  B01F13/0059 ,  G01N2030/347 ,  G01N2030/381 ,  G01N2030/387 ,  G01N30/02 ,  Y10S435/808 ,  Y10S436/805 ,  Y10T436/111666 ,  Y10T436/144444

Abstract: The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent and optically measuring a parameter in the incubated reaction mixture. The mixing, incubating and measuring are carried out during the action of a centrifugal force which forces the solution through a plurality of interconnected small hollow spaces having flow resistance so adapted with regard to one another as to mix the reaction components. Incubation may also take place before the reaction solution passes from the plurality of interconnected small hollow spaces into a measuring chamber in which the measurement is carried out.

Abstract translation: 本发明提供了一种用于通过将样品溶液与至少一种试剂混合和孵育并光学测量培养的反应混合物中的参数来进行分析测定的方法。 混合，孵育和测量在离心力的作用期间进行，其离心力迫使溶液通过多个互相连接的小空心空间，其具有相对于彼此适应的流动阻力以混合反应组分。 反应溶液也可以在反应溶液从多个相互连接的小中空空间通过进入测量室的测量室之前进行。

公开(公告)号：US3649498A

公开(公告)日：1972-03-14

申请号：US3649498D

申请日：1967-08-02

Applicant: PRETORIUS VICTOR ,  HAHN HANS HELMUT

Inventor： PRETORIUS VICTOR ,  HAHN HANS HELMUT

IPC: B01D11/02 ,  B01D11/04 ,  B01D15/00 ,  B01D15/08 ,  B01D53/18 ,  G01N27/447 ,  G01N27/49 ,  G01N30/02 ,  G01N30/06 ,  G01N30/14 ,  G01N30/34 ,  G01N30/38 ,  G01N30/50 ,  G01N30/56 ,  G01N30/62 ,  G01N30/64 ,  G01N30/84 ,  B01K5/00 ,  B01K3/00

CPC classification number: B01D69/10 ,  B01D11/02 ,  B01D11/04 ,  B01D15/08 ,  B01D53/18 ,  G01N27/404 ,  G01N27/447 ,  G01N30/02 ,  G01N30/06 ,  G01N30/14 ,  G01N30/34 ,  G01N30/38 ,  G01N30/50 ,  G01N30/56 ,  G01N30/64 ,  G01N30/84 ,  G01N2030/387 ,  G01N2030/626 ,  G01N2030/645 ,  G01N2030/0035 ,  B01D15/36 ,  B01D15/361

Abstract: THE PROGRESS OF A CHROMATOGRAPHIC SEPARATION IS MONITORED AT ANY DESIRED LOCALITY OF THE CHROMATOGRAPHIC SYSTEM BY OBSERVING AT A SOLID DETECTION ELECTRODE WITH REFERENCE TO A REFERENCE ELECTRODE ANY DETECTABLE CONSTITUTION AND CONCENTRATION DEPENDENT ELECTRICAL QUANTITY, E.G. CONDUCTIVITY, ELECTROLYTIC CURRENT, CAPACITANCE, ZETA-PHENOMENA OR A COMBINATION OF SUPERIMPOSED QUANTITIES.

公开(公告)号：US3182394A

公开(公告)日：1965-05-11

申请号：US22735062

申请日：1962-10-01

Applicant: BODENSEEWERK PERKIN ELMER CO

Inventor： DIETRICH JENTZSCH

IPC: B01D53/02 ,  G01N30/38 ,  G01N30/50 ,  G01N30/56 ,  G01N30/60

CPC classification number: G01N30/6078 ,  B01D53/025 ,  G01N30/50 ,  G01N30/56 ,  G01N30/6052 ,  G01N30/6073 ,  G01N2030/387 ,  Y10S228/903 ,  Y10T29/49888

公开(公告)号：US08497471B2

公开(公告)日：2013-07-30

申请号：US13232861

申请日：2011-09-14

Applicant: William F. Haddon ,  Nigel J. Clarke

Inventor： William F. Haddon ,  Nigel J. Clarke

IPC: B01D59/44

CPC classification number: B01D59/44 ,  G01N30/7233 ,  G01N33/6848 ,  G01N2030/387 ,  G01N2030/8831

Abstract: Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.

Abstract translation: 描述了用于测量样品中甲基化TPMT酶产物的量的方法。 更具体地，描述了用于在测试样品中利用质谱技术检测和定量6-MMP或同位素标记的6-MMP的质谱法，并且使用这些方法来测定样品中存在的TPMT酶的活性。

公开(公告)号：US08039794B2

公开(公告)日：2011-10-18

申请号：US12336499

申请日：2008-12-16

Applicant: William F. Haddon ,  Nigel J. Clarke

Inventor： William F. Haddon ,  Nigel J. Clarke

IPC: B01D59/44

CPC classification number: B01D59/44 ,  G01N30/7233 ,  G01N33/6848 ,  G01N2030/387 ,  G01N2030/8831

Abstract: Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.

Abstract translation: 描述了用于测量样品中甲基化TPMT酶产物的量的方法。 更具体地，描述了用于在测试样品中利用质谱技术检测和定量6-MMP或同位素标记的6-MMP的质谱法，并且使用这些方法来测定样品中存在的TPMT酶的活性。