公开(公告)号：US20140083856A1

公开(公告)日：2014-03-27

申请号：US14115976

申请日：2012-05-04

申请人： Dimitrios Sideris ,  Alex Iles ,  Richard Jackson

发明人： Dimitrios Sideris ,  Alex Iles ,  Richard Jackson

IPC分类号： G01N27/447

CPC分类号： G01N27/44791 ,  B01D57/02 ,  G01N27/447 ,  G01N27/44713 ,  G01N27/44756 ,  Y10T29/49002

摘要： A device comprises an electric field applying assembly adapted to generate an electric field having a discrete electric field profile; a conducting volume and an electrical interface region provided between the conducting volume and the electric field applying assembly such that the discrete electric field is applied to the material by the electric field applying assembly at a location spaced from the conducting volume, wherein the electrical interface region comprises at least an ionically conductive material arranged adjacent to an in contact with the conducting volume; such that the discrete electric field applied by the electric field applying assembly is smoothed by the electrical interface region so that the electric field profile established within the conducting volume is substantially continuous.

摘要翻译： 一种装置包括适于产生具有离散电场分布的电场的电场施加组件; 导电体积和电接口区域，设置在导电体积和电场施加组件之间，使得离散电场通过电场施加组件在与导电体积隔开的位置处施加到材料，其中电接口区域 至少包括邻近与导电体接触的离子导电材料; 使得由电场施加组件施加的离散电场由电接口区域平滑，使得在导电体积内建立的电场分布基本上是连续的。

公开(公告)号：US09719962B2

公开(公告)日：2017-08-01

申请号：US14115976

申请日：2012-05-04

申请人： Dimitrios Sideris ,  Alex Iles ,  Richard Jackson

发明人： Dimitrios Sideris ,  Alex Iles ,  Richard Jackson

IPC分类号： G01N27/447 ,  B01D57/02

CPC分类号： G01N27/44791 ,  B01D57/02 ,  G01N27/447 ,  G01N27/44713 ,  G01N27/44756 ,  Y10T29/49002

摘要： A device comprises an electric field applying assembly adapted to generate an electric field having a discrete electric field profile; a conducting volume and an electrical interface region provided between the conducting volume and the electric field applying assembly such that the discrete electric field is applied to the material by the electric field applying assembly at a location spaced from the conducting volume, wherein the electrical interface region comprises at least an ionically conductive material arranged adjacent to an in contact with the conducting volume; such that the discrete electric field applied by the electric field applying assembly is smoothed by the electrical interface region so that the electric field profile established within the conducting volume is substantially continuous.

公开(公告)号：US08569688B2

公开(公告)日：2013-10-29

申请号：US13382908

申请日：2010-07-06

申请人： Dimitrios Sideris

发明人： Dimitrios Sideris

IPC分类号： H01J49/26

CPC分类号： H01J49/32

摘要： A mass spectrometer is disclosed, comprising: a chamber; an injection device adapted to inject charged particles into the chamber; and field generating apparatus. The field generating apparatus is adapted to establish at least one field acting on the charged particles, the at least one field having an angular trapping component configured to form at least one channel between a rotation axis and the periphery of the chamber, the at least one channel being defined by energy minima of the angular trapping component, the field generating apparatus being further adapted to rotate the angular trapping component about the rotation axis, whereby in use charged particles are angularly constrained along the at least one channel by the angular trapping component to rotate therewith, a centrifugal force thereby acting on the charged particles. The at least one field additionally has a radial balancing component having a magnitude increasing monotonically with increasing radius from the rotation axis, at least in the vicinity of the at least one channel, whereby in use charged particles move along the at least one channel under the combined influence of the centrifugal force and the radial balancing component to form one or more particle orbits according to the charge to mass ratios of the particles. The mass spectrometer further includes a detector configured to detect at least one of the particle orbits. Methods of mass spectrometry are also disclosed.

摘要翻译： 公开了一种质谱仪，包括：腔室; 适于将带电粒子注入所述腔室的注射装置; 和场产生装置。 场产生装置适于建立作用在带电粒子上的至少一个场，所述至少一个场具有角度捕获部件，其被配置成在旋转轴线和腔室周边之间形成至少一个通道，所述至少一个 通道由角捕获部件的能量最小值定义，场发生装置还适于围绕旋转轴旋转角捕获部件，由此在使用中，带电粒子通过角捕获部件沿着至少一个通道成角度约束， 与其一起旋转，由此作用在带电粒子上的离心力。 所述至少一个场还具有径向平衡部件，所述径向平衡部件至少在所述至少一个通道附近具有随旋转轴线增加的半径单调增加的幅度，由此在使用中带电粒子沿着至少一个通道移动 离心力和径向平衡组分的组合影响根据颗粒的质荷比形成一个或多个颗粒轨道。 质谱仪还包括被配置为检测至少一个颗粒轨道的检测器。 还公开了质谱法。

公开(公告)号：US06979390B2

公开(公告)日：2005-12-27

申请号：US10450498

申请日：2001-10-23

申请人： Giles Hugo William Sanders ,  John Hassard ,  Stuart Hassard ,  Dimitrios Sideris

发明人： Giles Hugo William Sanders ,  John Hassard ,  Stuart Hassard ,  Dimitrios Sideris

IPC分类号： C07K1/24 ,  C07K1/28 ,  G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44795 ,  C07K1/24 ,  C07K1/28 ,  G01N27/44773

摘要： A system for separating biological molecules includes a chip (10) on which there is an electrophoresis separation microchannel (16). At the start of the channel there is an isoelectric focusing section (14) containing a gel having a pH gradient. Molecules to be separated are placed in the focusing section (14) and move to an equilibrium position under the influence of an electric field. The electrophoretic state of the molecules is then changed, and a high voltage potential applied across the entire length of the focusing and separation sections. The molecules migrate under the influence of the electric field from the focusing section into the separation section where their position and velocity are tracked. From a knowledge of position and velocity, a user can determine both charge/mass ratio and isoelectric equilibrium point for each molecule. The gels can be replicated in the chip to allow parallel analysis, and hence comparisons, to take place.

摘要翻译： 用于分离生物分子的系统包括其上存在电泳分离微通道（16）的芯片（10）。 在通道的开始处，存在含有具有pH梯度的凝胶的等电聚焦部分（14）。 要分离的分子被放置在聚焦部分（14）中并在电场的影响下移动到平衡位置。 然后改变分子的电泳状态，并且在聚焦和分离部分的整个长度上施加高电压电位。 分子在电场的影响下从聚焦部分迁移到其位置和速度被跟踪的分离部分。 根据位置和速度的知识，用户可以确定每个分子的电荷/质量比和等电点平衡点。 凝胶可以在芯片中复制，以便进行并行分析，从而进行比较。

公开(公告)号：US20120181422A1

公开(公告)日：2012-07-19

申请号：US13382908

申请日：2010-07-06

申请人： Dimitrios Sideris

发明人： Dimitrios Sideris

IPC分类号： H01J49/26 ,  H01J49/20 ,  H01J49/04 ,  H01J49/22

CPC分类号： H01J49/32

摘要： A mass spectrometer is disclosed, comprising: a chamber; an injection device adapted to inject charged particles into the chamber; and field generating apparatus. The field generating apparatus is adapted to establish at least one field acting on the charged particles, the at least one field having an angular trapping component configured to form at least one channel between a rotation axis and the periphery of the chamber, the at least one channel being defined by energy minima of the angular trapping component, the field generating apparatus being further adapted to rotate the angular trapping component about the rotation axis, whereby in use charged particles are angularly constrained along the at least one channel by the angular trapping component to rotate therewith, a centrifugal force thereby acting on the charged particles. The at least one field additionally has a radial balancing component having a magnitude increasing monotonically with increasing radius from the rotation axis, at least in the vicinity of the at least one channel, whereby in use charged particles move along the at least one channel under the combined influence of the centrifugal force and the radial balancing component to form one or more particle orbits according to the charge to mass ratios of the particles. The mass spectrometer further includes a detector configured to detect at least one of the particle orbits. Methods of mass spectrometry are also disclosed.

摘要翻译： 公开了一种质谱仪，包括：腔室; 适于将带电粒子注入所述腔室的注射装置; 和场产生装置。 场产生装置适于建立作用在带电粒子上的至少一个场，所述至少一个场具有角度捕获部件，其被配置成在旋转轴线和腔室周边之间形成至少一个通道，所述至少一个 通道由角捕获部件的能量最小值定义，场发生装置还适于围绕旋转轴旋转角捕获部件，由此在使用中，带电粒子通过角捕获部件沿着至少一个通道成角度约束， 与其一起旋转，由此作用在带电粒子上的离心力。 所述至少一个场还具有径向平衡部件，所述径向平衡部件至少在所述至少一个通道附近具有随旋转轴线增加的半径单调增加的幅度，由此在使用中带电粒子沿着至少一个通道移动 离心力和径向平衡组分的组合影响根据颗粒的质荷比形成一个或多个颗粒轨道。 质谱仪还包括被配置为检测至少一个颗粒轨道的检测器。 还公开了质谱法。

公开(公告)号：US07425252B2

公开(公告)日：2008-09-16

申请号：US10344182

申请日：2001-07-20

申请人： Dimitrios Sideris

发明人： Dimitrios Sideris

IPC分类号： G01N27/447 ,  G01N27/453 ,  G01N30/02 ,  G01N33/48

CPC分类号： G01N27/44717

摘要： An analysis system for and method of enabling determination of the velocities of migrating objects, the system comprising an equiphase space-time map generator for generating an equiphase space-time map of equiphase points from each of a plurality of data sets representative of the signals detected at a plurality of spaced positions, with the velocities of the objects being determinable by fitting the sets of equiphase points in the space-time map corresponding to the respective objects.

摘要翻译： 一种能够确定移动物体的速度的分析系统和方法，该系统包括一个同相空间 - 时间映射发生器，用于从表示检测到的信号的多个数据集中的每一个产生等相位点的均相时空映射 在多个间隔位置，物体的速度可以通过在对应于各个物体的时空映射中拟合等位相点的集合来确定。

公开(公告)号：US20080083621A1

公开(公告)日：2008-04-10

申请号：US11813191

申请日：2005-12-22

申请人： Dimitrios Sideris

发明人： Dimitrios Sideris

IPC分类号： B01D57/02

CPC分类号： G01N27/44773 ,  G01N27/44713 ,  G01N27/4473

摘要： An electrophoresis method is provided for separating objects in a fluid contained in a separation channel. The method comprises: applying an electric field along the separation channel, the electric field having a field profile, and thereby causing at least some of the objects to move relative to the fluid; varying the applied electric field so as to adjust the field profile relative to the separation channel, thereby causing the objects to separate into bands under the combined influences of an electric force due to the electric field and a hydrodynamic force due to the fluid.

摘要翻译： 提供了用于分离包含在分离通道中的流体中的物体的电泳方法。 该方法包括：沿着分离通道施加电场，电场具有磁场分布，从而使至少一些物体相对于流体移动; 改变所施加的电场以便相对于分离通道调整磁场分布，从而在由于电场引起的电力和由于流体引起的流体动力的组合影响下使物体分离成带。

公开(公告)号：US06893878B2

公开(公告)日：2005-05-17

申请号：US10074297

申请日：2002-02-13

申请人： Dimitrios Sideris

发明人： Dimitrios Sideris

IPC分类号： G01N1/10 ,  B01D53/24 ,  B01D53/32 ,  B03C5/00 ,  B04B5/00 ,  B04B5/10 ,  C07K1/14 ,  C12N1/02 ,  C12N15/00 ,  G01N1/22 ,  G01N1/28 ,  G01N1/36 ,  G01N15/04 ,  G01N27/447 ,  G01N33/483 ,  G01N27/26

CPC分类号： G01N27/44756 ,  B04B5/10 ,  G01N15/042 ,  G01N2015/045 ,  Y10T436/111666 ,  Y10T436/25375

摘要： A centrifugal spectrometer has a solid rotor (10) formed within which there are cavities or blades (12). In use, each blade is filled with a buffer solution, and a sample to be separated is placed in a sample well (32) at the end of a separation channel (24). The rotor is spun at a controlled velocity and, at the same time, a controlled potential difference is applied along the length of the blade. The blade shape causes the resultant electric field to vary as a function of radial distance. The sample separates out into bands, which move along the channel (24) under the combined influence of the centrifugal force and the varying electric field. The bands focus at differing equilibrium points according to their charge/mass ratios. The band positions are determined by a readout head (36). The dynamic range of the device may be controlled by altering the rotational velocity and the voltages that are applied.

摘要翻译： 离心分光计具有形成有固体转子（10），其中有空腔或叶片（12）。 在使用中，每个叶片充满缓冲溶液，待分离的样品置于分离通道（24）末端的样品孔（32）中。 转子以受控的速度旋转，并且同时沿刀片的长度施加受控的电位差。 叶片形状使得所得的电场作为径向距离的函数而变化。 样品分离成带，其在离心力和变化的电场的组合影响下沿着通道（24）移动。 根据其电荷/质量比，带在不同的平衡点聚焦。 带位置由读出头（36）确定。 可以通过改变所施加的旋转速度和电压来控制装置的动态范围。

公开(公告)号：US07425251B2

公开(公告)日：2008-09-16

申请号：US10344070

申请日：2001-07-20

申请人： Dimitrios Sideris

发明人： Dimitrios Sideris

IPC分类号： G01N27/447 ,  G01N27/453 ,  G01N30/02 ,  G01N33/48

CPC分类号： G01N27/44721 ,  G01N27/44717 ,  G01N27/44791

摘要： A system and method of characterizing, in particular sequencing, polymeric samples, the system comprising: a space-time map generator for generating a space-time map of points representative of the signal peaks of signals detected at a plurality of spaced positions as passed by the migrating components of a plurality of separately-provided sample plugs; and a vertex finder for identifying vertices from the space-time map, with a single vertex being identified for the components of each separately-provided sample plug and the velocities of the sample components being determinable from the points in the space-time map.

摘要翻译： 一种表征聚合物样品的系统和方法，特别是对聚合物样品进行排序，所述系统包括：时空映射发生器，用于产生代表通过的多个间隔位置处检测到的信号的信号峰值的点的时空映射， 多个单独提供的样品塞的迁移组分; 以及用于从时空映射识别顶点的顶点查找器，其中为每个单独提供的样本插头的组件标识单个顶点，并且可以从时空映射图中的点确定样本组件的速度。

公开(公告)号：US08262884B2

公开(公告)日：2012-09-11

申请号：US11813191

申请日：2005-12-22

申请人： Dimitrios Sideris

发明人： Dimitrios Sideris

IPC分类号： G01N27/447 ,  G01N27/453 ,  B01D57/02

CPC分类号： G01N27/44773 ,  G01N27/44713 ,  G01N27/4473

摘要： An electrophoresis method is provided for separating objects in a fluid contained in a separation channel. The method comprises: applying an electric field along the separation channel, the electric field having a field profile, and thereby causing at least some of the objects to move relative to the fluid; varying the applied electric field so as to adjust the field profile relative to the separation channel, thereby causing the objects to separate into bands under the combined influences of an electric force due to the electric field and a hydrodynamic force due to the fluid.

摘要翻译： 提供了用于分离包含在分离通道中的流体中的物体的电泳方法。 该方法包括：沿着分离通道施加电场，电场具有磁场分布，从而使至少一些物体相对于流体移动; 改变所施加的电场以便相对于分离通道调整磁场分布，从而在由于电场引起的电力和由于流体引起的流体动力的组合影响下使物体分离成带。