利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

24 条记录 (耗时 0.041 秒)

    Method and Apparatus for Pipette Tip Columns
    1.
    发明申请
    Method and Apparatus for Pipette Tip Columns 有权
    移液管柱的方法和装置

    公开(公告)号：US20110195518A1

    公开(公告)日：2011-08-11

    申请号：US13023519

    申请日：2011-02-08

    申请人： Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    发明人： Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    IPC分类号： G01N1/18 ,  B01L3/02

    CPC分类号： B01L3/02 ,  B01L3/0275 ,  B01L2200/0631 ,  B01L2300/0681 ,  B01L2400/0487 ,  Y10T436/255

    摘要： An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation.

    摘要翻译： 开发了一种使用带有移液管尖端柱的移液器的装置和方法，其中移液管在移液管尖端柱插入微量培养板的孔中进行操作。 在该配置中，移液管是自由站立的，并且基本上垂直于微孔板。 移液管尖端柱的开放的下端大致在板孔内部居中。 柱和板被设计成使得移液管尖端柱的开口下端与板孔中的液体接触，但是柱不会密封在井底，防止流入和流出柱。 移液器包含适当的固件和软件，用于控制移液管尖端柱操作的所有步骤的流量。

    Method and apparatus for pipette tip columns
    4.
    发明授权
    Method and apparatus for pipette tip columns 有权
    吸头柱的方法和设备

    公开(公告)号：US09242244B2

    公开(公告)日：2016-01-26

    申请号：US13023519

    申请日：2011-02-08

    申请人： Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    发明人： Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    IPC分类号： B01L3/02 ,  G01N1/18

    CPC分类号： B01L3/02 ,  B01L3/0275 ,  B01L2200/0631 ,  B01L2300/0681 ,  B01L2400/0487 ,  Y10T436/255

    摘要： An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation.

    摘要翻译： 开发了一种使用带有移液管尖端柱的移液器的装置和方法，其中移液管在移液管尖端柱插入微量培养板的孔中进行操作。 在该配置中，移液管是自由站立的，并且基本上垂直于微孔板。 移液管尖端柱的开放的下端大致在板孔内部居中。 柱和板被设计成使得移液管尖端柱的开口下端与板孔中的液体接触，但是柱不会密封在井底，防止流入和流出柱。 移液器包含适当的固件和软件，用于控制移液管尖端柱操作的所有步骤的流量。

    Method and Apparatus for Pipette Tip Columns
    5.
    发明申请
    Method and Apparatus for Pipette Tip Columns 有权
    移液管柱的方法和装置

    公开(公告)号：US20160061697A1

    公开(公告)日：2016-03-03

    申请号：US14846825

    申请日：2015-09-07

    申请人： Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    发明人： Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    IPC分类号： G01N1/34 ,  B01L3/00 ,  B01L3/02

    CPC分类号： G01N1/34 ,  B01L3/021 ,  B01L3/0237 ,  B01L3/0275 ,  B01L3/5085 ,  B01L2200/025 ,  B01L2200/0631 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0487 ,  G01N1/40 ,  Y10T436/255

    摘要： An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The pipette is hand-held when transferring between positions, e.g., between operation steps that take place in different rows of wells.The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation.In some embodiments, cells are purified from biological samples.

    摘要翻译： 开发了一种使用带有移液管尖端柱的移液器的装置和方法，其中移液管在移液管尖端柱插入微量培养板的孔中进行操作。 在该配置中，移液管是自由站立的，并且基本上垂直于微孔板。 移液器在位置之间转移时是手持式的，例如在不同排井中发生的操作步骤之间。 移液管尖端柱的开放的下端大致在板孔内部居中。 柱和板被设计成使得移液管尖端柱的开口下端与板孔中的液体接触，但是柱不会在井底密封，从而防止流入和流出柱。 移液器包含适当的固件和软件，用于控制移液管尖端柱操作的所有步骤的流量。 在一些实施方案中，细胞从生物样品中纯化。

    Method and Device for Gravity Flow Chromatography
    9.
    发明申请
    Method and Device for Gravity Flow Chromatography 审中-公开
    重力流动色谱法和装置

    公开(公告)号：US20100140173A1

    公开(公告)日：2010-06-10

    申请号：US12709487

    申请日：2010-02-21

    申请人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    发明人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    IPC分类号： B01D15/20 ,  B01D15/22

    CPC分类号： C07K1/16 ,  B01J20/286 ,  B01J20/287 ,  B01J20/3244 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/64 ,  C12N15/1006 ,  G01N1/405 ,  G01N30/02 ,  G01N30/466 ,  G01N30/603 ,  G01N30/6043 ,  G01N30/6065 ,  G01N30/6091 ,  G01N2030/062 ,  G01N2035/103 ,  G01N2035/1053 ,  B01D15/3804 ,  B01D15/34

    摘要： The invention provides gravity chromatographic columns for the automated purification of a material (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the columns employ modified plates or racks as column bodies. In some embodiments, the invention provides methods and devices for gel filtration, desalting, buffer exchange, ion exchange, ion-pairing, normal phase and reverse phase chromatography. In some embodiments, the invention provides multiplexing gravity flow chromatography on a liquid handling robotic system.

    摘要翻译： 本发明提供用于从样品溶液自动纯化材料（例如生物大分子，如肽，蛋白质或核酸）的重力色谱柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于底部玻璃料之上或底部和顶部玻璃料之间的介质床。 在一些实施方案中，柱使用改进的移液管尖作为柱体。 在一些实施例中，列使用改性板或架作为柱体。 在一些实施方案中，本发明提供了用于凝胶过滤，脱盐，缓冲液交换，离子交换，离子配对，正相和反相色谱法的方法和装置。 在一些实施方案中，本发明提供了液体处理机器人系统上的多重重力流色谱法。

    Highly Parallel Gel-Free Cloning Method
    10.
    发明申请
    Highly Parallel Gel-Free Cloning Method 审中-公开
    高度平行的无凝胶克隆方法

    公开(公告)号：US20100129873A1

    公开(公告)日：2010-05-27

    申请号：US12625535

    申请日：2009-11-24

    申请人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde ,  David P. Hornby

    发明人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde ,  David P. Hornby

    IPC分类号： C12P19/34

    CPC分类号： C12N15/64 ,  C12N15/1093 ,  C12N15/66

    摘要： A highly parallel method for gene cloning is presented. PCR products can be isolated using a solid phase and ligated into a positive selection vector. The cloning method has a very high success rate and can be performed entirely by a liquid handling robot with very little human intervention.

    摘要翻译： 提出了一种高度并行的基因克隆方法。 可以使用固相分离PCR产物并连接到阳性选择载体中。 克隆方法具有非常高的成功率，并且可以完全由液体处理机器人完成，人为干预很少。